病原性 大腸菌이 생성하는 耐熱性 腸毒素의 抗體 생성조건 및 中和效果

金敎昌,都大洪,鄭埈永 충북대학교 농업과학기술연구소 1993 農業科學硏究 Vol.11 No.1

The heat-stable enterotoxin(ST) producing Escherichia coli strains were isolated from the swine. The purified ST was immunized with ST-BSA conjugate and obtained antiserum from a rabbit (New Zealand Whit). A pathogenic toidity was neutralized with anti-rabbit serum. When antiserum obtained from rabbits immunized with ST- BSA antigens. It was production the most of antiserum at the fourth immunization (21 days). Obatined antisera neutralized the toxic activity of purified 4 mouse units to 1 : 32 of antisera dilution folds.

항생제 다제내성균 Staphylococcus aureus SA2로부터 분리한 테트라사이클린 내성 플라스미드 pKH6의 염기서열

이대운,윤성준,김우구,신철교,임성환,이백락,문경호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

한국에서 임산균주로부터 분리한 테트라사이클린(Tc) 내성 플라스미드 pKH6의 전체 염기서열을 결정하여 가장 대표적인 Tc 내성 플라스미드인 pT181과 비교하였다. pKH6의 전체 길이는 4439bp로 pT181과 동일하게 나타났으며 염기서열은 7개의 염기에서 차이를 보였다. 전부 다 염기쌍이 치환된 형태로 나타났으며 그 중 3개는 coding 부위에서 일어났다. coding 부위에서 일어난 경우에도 1개는 동일한 아미노산을 coding 하였기 때문에 pT181과 차이가 없었으며 RepC와 Pre 단백질에 있어 각각 1개의 아미노산에서 차이를 나타내었다. The complete nucleotide sequence of pKH6, a tetracycline-resistance (Tc^r) plasmid isolated from multi-drug resistant Staphlococcus aureus SA2, has been determined and compared with that of the staphylococcal Tc^r plasmid pT181. The nucleotide sequences of the two plasmids are in agreement except for 7 nucleotides. All differences are caused by base pair substitutions. Among 6 substitutions, 3 occurred in coding regions. However, only tow base substitutions in coding regions resulted in changes of amino acid sequences in two different ORFs of repC and Pre proteins.

우리나라 政府出捐 硏究機關의 外國技術 導入 經路選擇의 決定要因에 관한 硏究

金敎祥,趙大宇 韓國國際經營學會 1992 國際經營硏究 Vol.3 No.1

우리나라는 `90년대 산업의 질적 고도화와 지속적인 경제성장을 뒷받침하기 위하여 科學技術의 중요성이 요구되고 있다. 이를 실질적으로 뒷받침하기 위한 방법의 하나로서 政府出捐 硏究機關들이 외국으로부터 산업기술 및 공공기술에 필요한 기술도입계약과 함께 정부 특정연구과제로서 國際共同硏究와 아울러 國際技術協力 등을 중심으로 한 公共技術協力을 추진하고 있다. 민간제조기업의 기술도입경로가 海外直, 合作投資 및 技術導入契約 등인 데 비해 정부출연연구기관의 기술도입경로는 기술도입계약외에 公共技術協力 (이는 국제공동연구, 국제기술협력 등으로 대별됨)이라는 상이한 경로를 밟고 있다. 그러나 국제간 기술이전에 대한 연구의 대부분이 민간제조기업에 의해 수행되는 현상을 연구해온 데 대해 본 연구에서는 이미 중요한 기술도입주체로 부각되어 향후 우리나라를 기술대국으로서의 기반을 짊어져야 하는 주체로서의 정부출연연구기관을 연구대상으로 하여 기술도입경로선택에 영향을 미치는 결정요인을 규명해 보자는 데 그 의의가 있다. 이를 위해서는 연구기관이 갖는 特有性(uniqueness)을 smoothe시키고 普遍性(universality)을 전제로 하기 위해 새로이 구성하였다. 한편 기술도입경로도 민간기업과는 달리 기술도입계약, 공공기술협력(국제공동연구, 국제기술협력)등으로 재편하였다. 보다 보편적인 決定要因을 추출하기 위해 독립변수군을 기술특성군, 기술제공특성군, 기술도입자특성군, 정부특성군 등으로 4대분류하여, 기술도입경로선택의 결정요인을 檢證하였다. 결론적으로 정부출연연구기관의 外國技術導入經路의 選澤 및 說明變數로는 다수 (예: 기술수명 주기상의 위치, 기술연령, 기술도입담당부서, 국제협정지원 등) 가 선정될 수 있으나 그 중에서 技術의 垂直的 位階分類가 가장 중요한 결정요인임을 밝혔다. 즉 정부출연연구기관의 개별성 및 특유성에 관계없이 도입기술이 기술위계상 基礎基盤技術일 경우에는 國際共同硏究, 應用技術일 경우에는 國際技術協力, 常用化技術일 경우에는 技術導入契約이라는 經路를 선택하는 성향을 검증했다는 점이다. 이 연구결과는 정부출연연구기관의 경우 기술위계상 특성에 따라 외국으로부터 기술도입경로를 선택할 수 있는 기준을 제시할 수 있다는 데 그 의의가 있다고 본다.

돼지에서 由來한 耐熱性 腸毒素生産 大陽菌의 分離 및 判定

都大洪,金敎昌,金道榮 충북대학교 농업과학기술연구소 1987 農業科學硏究 Vol.5 No.2

Enterotoxigenic E.coli is the one of major causative occured to the infantile swine diarrhea. A total of 189 isolated idem assumed E. coli, Salmonella and Shigella was isolated from swine in Kyong-Gi and Chung-Cheong province. When enterotoxigenic E. coli was infected to infant swine, these adhered to the mucosa of small intestine with pili and excrete enterotoxin, which caused the diarrhea. The detection of a heat-stable enterotoxin(ST) produced of isolated E.coli was porformed by infant mouse assay, and was identified pili by the aggutination test of rbc suspention. The plasmid DNA profile was investigated by the agarose gel electrophoresis. The results of assay to enterotoxigenic E.cofi summarized as follow. Isolation ratio of enterotoxigenic E.coli among total of 45 isolated strains was taken 56.52% during summer. Four strains(KS-4, KM-7, KM-12, CM-7) of high produced ST were showed the G/B ratio above 1.10 by infant mouse assay. Four strains of high produced enterotoxigenic E.coli was isolated from swine with diarrhea, and 3 strains (KS-4, KM-7, KM-12) were proved to possess K88 and K99 antigens, or K88 antigen. The patters of plasmid DNA was contained 4.5kb plasmids encoded for ST. The antibiotics sensitivity patters of cultured enterotoxigenic E.coli were showed a tendency to be resistant against tetracycline and streptomycin.

분꽃씨의 抗알레르기 效果에 대한 硏究

鄭大琪,宋昊埈,辛民敎 대한본초학회 1997 大韓本草學會誌 Vol.12 No.1

This study was investigated the inhibitory activity of Semen Mirabilis (SM) (Nyctaginaceae) on type 1 immediate hypersensitivity of the anaphylactic type. 1 used a mouse model of passive cutaneous anaphylaxis, an IgE-mediated, mast cell-dependent reaction. Administered orally at 250, 500 ㎎/㎏ body weight 1 h before the challenge, SM was potently inhibited PCA in mouses in which disodium cromoglycate showed poor inhibitory activity. SM inhibited compound 48/80-induced anaphylaxis 94.21% with a dose of 0.5g/㎏ body weight at 1 h before or 5 and 10 min after injection of compound 48/80. SM (0.03-2.0 ㎎/㎖) also exhibited the dose-dependent inhibitory effect on compound 48/80-induced histamine release from rat peritoneal mast cells. Moreover, it was clearly demonstrated that SM and disodium cromoglycate disodium cromoglycate potently inhibited such type I allergic reactions as anaphylactic shocks, suggesting that these drugs, at least in part, share the same mechanism of action. It is suggested that SM may exert a stronger inhibition on the mast cell degranulation process. When I examined the effect of SM on the release of IgE in mouse spleen whole cells and human mycloma U266B1, the release of IgE is inhibited by Mirabilis jalapa L. These results suggest the possibility that the inhibition of allergic reaction by SM is regulated by histamine and IgE.

數種 木材腐朽菌의 菌絲 發育에 미치는 防腐劑의 效果

鄭大敎 건국대학교 1967 學術誌 Vol.8 No.1

(1) The sterilizing effects of NaF, CuSO4, CuCO3, Hgcl2, ZnSO4, and Coaltar Creosote on the mycelial growth of Irpex Censors Berk Polystictus Versicolor L. Fr, Polystictus Versicolor L. Var Nigricans, and Schigophyllum Commune Fr. were studied. (2) The range of minimum density of the fungicides to check. The growth of four fungi mentioned above was as follow : NaF 0.15∼0.25(%) CuCO3 0.40∼0.50(%) ZnSO4 0.40∼0.45(%) CuSO4 0.20∼0.35(%) Hgcl2 0.05(%) Creosote 0.10∼0.15(5) of the fungicides tested, Hgcl2 was most effective in fungicidal effects, and Creosote, NaF, CuSO4, ZnSO4, CuCO3, followed. The order of resistance of the fungi to the fungicides was as follows: S, Commune Fr, P, Versicolor L. Var, nigricans I, Censors Berk P, Versicolor L. Fr (3) The fungicides were added to the Pepton-ager culture medium at the concentration between 0.01 and 0.5%, and the medium was filled into 9cm Petridishes, two square millimeter ager blocks prepaered separately from the fungi contained ager were placed in the middle of the Petri-dishes, incubated six days at 26℃. Diameter of biggest colonies were measured.

핵저지능이 열자극전류에 미치는 영향

邊大鉉,趙昌熙,韓斗敎 弘益大學校 1987 弘大論叢 Vol.19 No.2

In this study induced thermally stimulated current by the TSC was measured by the open circuit method after implanting Na+ ion into a PET film. With the result, using a new theory, activated energy, escape frequency, relaxation time and ion density was evaluated. (-) peak appeared at 363 K and 390 K when temperature increasing rate was 0.1 K/sec. and a (+) peak in the range 310~350 K. As a result of analysis, the peak at 363 K was by Na^(+) ion trapped in the surface trap and the activated energy 1. 14eV. 390 K peak had 1.66 eV activated energy and was by Na+ ion trapped in a vacance. The TSC current of 390 K peak was proportional to nuclear stopping power.

韓國産 가지과 植物에 관한 本草學的 硏究

정대기,변종호,신민교,송호준 대한본초학회 1998 大韓本草學會誌 Vol.13 No.1

For the purpose of developing Korean Herbalogy with the plants of Solanceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been throughly investigative and the results obtained were as follows: 1. There were totaled to 13 genera and 26 species in Korea and among them medicinal plants are 11 genera, 19 species, some 73% in total but, the number of species may be added because of the similiar plants. 2. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Solanaceae family were classified as Radix 8, Fructus 8, Herba 6 ,Foilum 5, Semen 3, Lignum and Flos 2. 3. According to sum of 26 species in Solanaceae family, they were classified into Solanum genera 8, Physalis and Capsicum genera 3 each, Datura and Nicotiana genera 2 each. Thus it was noticed that Solanum genera was the main kind, some 30% in total. 4. According to the number of species of the origin plants about each chinese materia medicals, they were calssified into HERBA SOLANI LYRATI 3, FRUCTUS CAPSICI and FLOS DATURAE 2 each. 5. According to nature and flavour of medicinal plants, they were classified into cold, cool; 17 each,warmth,heat; 8 each, balance 5 and unidentified 6. Thus it was noticed that cold and cool is the main in nature and flavour of medicinal plants. 6. According to the Properties and Principal curative action, they were classified into Drugs for treating febrifugal and detoxicant 14, Drugs for relieving pain 7, Antitussives . Diuretics . Drugs for invigorating blood circulation. Tonics 4 each, Anthelmintics 3. 7. Comparing to whole medicinal plants 19 kinds, toxic drugs include minor toxin were 3 kinds, 16% of the whole. From this result, It was revealed that the plants for medical purpose in Solanaceae was 73% of the whole, in which RADIX and FRUCTUS were abundunt. It is considered that many experiments and clinical approaches must be continued to use Solanaceae plants widely.

국내 주요기업의 웹사이트 관리에 관한 탐색적 연구

서창교,권대윤 慶北大學校 出版部 2001 經商論集 Vol.29 No.1

자생활잡목의 用送開發을 위한 比較耐朽性에 관한 연구

鄭大敎 建國大學校附設 農業資源開發硏究所 1980 論文集 Vol.5 No.-

本 試驗은 木材腐朽菌 Poria vaporaria(per) Fr. Merlius lacrymans (Jacq) Fr. Schigophyllum commune Fr. Trametes pini Fr. Paxillus pannoides Fr. Daedalla quercina (L) Pers. 등 6株와 한국自生 활엽수인 신갈나무·졸참나무·자작나무·오리나무·느릅나무·벚나무·물푸레나무·다릅나무·버드나무·느티나무·층층나무 등 11種에 대한 比較耐朽性을 試驗하였던 바 다음과 같은 結果를 얻었다. 1) Poria vaponania 에 대한 樹種別 耐朽度는 물푸레나무의 邊材가 5.57%로 가장 높았으며 다음이 느티나무 心材 8.53%, 벚나무 心材 8.54%의 順位였으며 가장 약한 樹種은 버드나무 35.07이며, 다음이 신갈나무 24.99%, 졸참나무 24.25%이며, 기타는 中位였다. 2) Merliuo lacrymans에 대한 樹種別 耐朽度는 느티나무 心材가 9.38%로 가장 높았으며 다음이 느릅나무 邊材 10.18%, 자작나무 心材 12.23%의 順位였으며 가장 약한 樹種은 층층나무 邊材 27.14%였고 다음은 버드나무 邊材 24.58%, 다릅나무 17.72%의 順이었고 기타는 中位였다. 3) Schizophyllun commmune에 대한 耐朽度는 느티나무 心材 11.75%로서 가장 강하였고 다음이 느릅나무 邊材 12.19%, 자작나무 心材 12.88%의 順位였고 가장 약한 樹種은 층층나무 邊材 32.88%였으며, 다음은 버드나무 24.63%, 졸참나무 18.91%의 順이었다. 기타는 中位였다. 4) Trametes pini에 대한 耐朽度는 느티나무 心材 23.4%로 가장 강하였으며, 다음이 다릅나무 心材 5.33%, 오리나무 10.84%의 順이었고, 가장 약한 樹種은 층층나무 邊材 33.19%이며, 다음이 물푸레나무의 邊材 19.25%, 졸참나무 心材 19.21%였고, 기타는 中位였다. 5) Paxillus pannoides에 대한 耐朽度는 느티나무 心材 1.73%로 가장 강하였고, 다음이 버드나무 邊材 3.75%, 느릅나무 邊材 6.08%였으며, 가장 약한 樹種은 다릅나무 心材 39.12%, 층층나무 邊材 37.89%, 신갈나무 23.65%였고 기타는 中位였다. 6) Daeualea quercina에 대한 耐朽度는 느티나무 心材 1.15%로 가장 높았으며, 다음이 벗나무 心材 3.04%, 느릅나무 邊材 3.15%의 順이었으며, 가장 약한 수종은 층층나무 邊材 32.00%, 버드나무 邊材 26.24%, 졸참나무 心材 22.36%의 順이었고 기타는 中位였다. 7) 苗種別로는 Paxillus pannoides가 腐朽力이 가장 강하였으며 다음이 Poria vaporaria Merlius lacrymans의 順이었고 Daedalea quercina가 가장 약한 편이었고 기타는 中位였다. This investigation was taken on the relative durability of 11 species of Korean indigenous hard wood such as Quercus mongolica Fischen ex. Turczanizow to the 6 strains of wood destroying fungi such as Poria vaporica (Per) Fr. The results obtained are as follow: 1. The relation durability of each species to Poria vaporica (Per) Fr. was varied. Fraxinus synchophylla Hance had highest durability (5.57%)and next orders were Zelkowa serrata makino (8.53%) and Prunus serrata Lindl (8.54%) in durable order. The lowest species was Salix horeensis Anderson (35.07%)and next were Quercus mongolica F. ex T. (24.99%) and Quercus serrata Thunbergi (24.25%). And other species were mediem. 2. The relative durability of each species to Merlius lacrymans was varied too. Zelkowa ressata M. had heighest durability (8.85%) and nest were Ulmus Davidiana Planchon var.japonica Nakai (10.18%) and Betula platyphylla Sukatchew var.latifolia nakai (12.23%) in durable order. The lowest species was cornus controverse Hemsley (27.14%) and next were Salix koreensis A. (24.58%) and Maackia amusensis Pruprecht et. Maximowiczia (17.72%) and others were middle degree. 3. The relative durability of each species to Schizophyllum commune was different from each other. Zelkowa serrata M. had highest durability (11.75%) and next orders were Ulmus Davidiana P.var japonica N. (12.19%) and Betula platyphylla S.var latifolia N. (12.88%)in durable order. The lowest species was Cosnus controversa H. (32.85%) and next were Salix koreensis A. (24.63%) and Quercus serrata T. (18.91%). And others were mediun 4. The relative durability of each species to Trametes Pini was varied. Zelkowa ressata M. had highest durability (2.34%) and next were Maackia amurensis R. et. M (5.33%) and alnus japonica Steudel (10.84%) in durable order. The lowest species was Cornus controverssa H. (33.19%) and next were Fraxinus rynchophylla H.(19.25%) and Quercus serrata T (19.21%). Other species were middle degree. 5. The relative durability of each species to Paxillus Pannoides was differ from each other. Zelkowa serrata M. had highest durability (1.93%) and next orders were Salix koreensis A (3.95%) and Ulmus Davidiana P.var japonica N. (6.08%) in durable order. The lowest species was cosnus controversa H. (37.84%) and next were Maackia amusensis R. et. M. (39.12%) and Quercus mongolica F. ex. T. (23.65%). Others were nedium. 6. The relative durability of each species to Daedalea quercine was various. Zelkowa serrata M. had highest durability (1.15%) and next were prunus serrata L. (3.04%) and Ulmus Davidiana P. (3.15%)in durable order. The lowest species was cornus controversa H. (32.00%) and next were Salix koreensis A. (26.24%) and Quercus serrata T. (22.36%). Others were medium. 7. Among the fungi, Paxibus Dannoides had most powerful decaying potency and next were Merlius lacrymans and Daedalea in decaying potency order. Other strains were middle degree. 8. Heart wood had relatively higher durability than sap wood and hard species was more durable than soft species. This investigation showed that there were many difference among tree species or fungi strains in themselves.