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Molecular characterization of the human ABO blood group orthologus system in pigs
Nguyen, D. T.,Choi, H.,Jo, H.,Kim, J.x2010,H.,Dirisala, V. R.,Lee, K.x2010,T.,Kim, T.x2010,H.,Park, K.x2010,K.,Seo, K.,Park, C. Blackwell Publishing Ltd 2011 Animal genetics Vol.42 No.3
<P><B>Summary</B></P><P>The selection and use of animals with blood group 0 in the process of transplanting pig organs or tissues into humans can positively contribute to the control of acute immune rejection due to differences in blood groups. Exon‐specific PCRs for the <I>porcine blood group A transferase</I> gene against genomic DNA from either blood group A or 0 animals resulted in the amplification failure of the <I>A0 blood group</I> gene exon 8 from blood group 0 animals. To characterize the genetic abnormality in the genome of blood group 0 animals, we screened bacterial artificial chromosome (BAC) clones from a Korean native pig BAC library which had the blood group 0 allele, and carried out shotgun sequencing. The analysis showed that the 0 allele has a large deletion between exon 7 of the <I>A0</I> blood group gene and the neighbouring <I>SURF6</I>. We also showed that the ABO blood group antigens in humans and the A0 blood group antigens in pigs are coded by mutations within the orthologous <I>glycosyltransferase</I> gene. In addition, we developed a multiplex genotyping method for the porcine <I>A0</I> blood group gene.</P>
Transfer‐Printing of As‐Fabricated Carbon Nanotube Devices onto Various Substrates
Thanh, Quy Nguyen,Jeong, Huiseong,Kim, Jinwoong,Kevek, J. W.,Ahn, Y. H.,Lee, Soonil,Minot, Ethan D.,Park, Jix2010,Yong WILEY‐VCH Verlag 2012 ADVANCED MATERIALS Vol.24 No.33
<P><B>Exact replicas of carbon nanotube devices</B> as fabricated on SiO<SUB>2</SUB>/Si substrates are prepared on various non‐conventional substrates such as nonplanar or soft substrates (see images) by a simple, yet versatile, transfer‐printing “cut‐and‐paste” method. In this way, harsh growth and fabrication processes can be minimized on the target substrates. The electrical characteristics of transfer‐printed devices are compared to those of original devices.</P>
Jo, Jang,Pouliot, Jeanx2010,Ré,mi,Wynands, David,Collins, Samuel D.,Kim, Jin Young,Nguyen, Thanh Luan,Woo, Han Young,Sun, Yanming,Leclerc, Mario,Heeger, Alan J. WILEY‐VCH Verlag 2013 ADVANCED MATERIALS Vol.25 No.34
<P><B>Power conversion efficiency up to 8.6%</B> is achieved for a solution‐processed tandem solar cell based on a diketopyrrolopyrrole‐containing polymer as the low‐bandgap material after using a thin polyelectrolyte layer to modify the electron‐transport ZnO layers, indicating that interfacial engineering is a useful approach to further enhancing the efficiency of tandem organic solar cells.</P>
Numerical study of the indentation formation of a compound droplet in a constriction
Hoe D. Nguyen,Truong V. Vu,Phan H. Nguyen,Binh D. Pham,Nang X. Ho,Cuong T. Nguyen,Vinh T. Nguyen 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.4
A compound droplet deforming in a constricted tube widely appears in drug delivery and microfluidic devices. In such a constriction, an indentation can present at the trailing surface of the droplet. However, this aspect has not been fully investigated and understood so far. This study focuses on the effects of some dimensionless parameters on the negative curvature, i.e., indentation, at the trailing surface of a compound droplet moving through a constricted tube. The presence of the constriction at the middle of the tube length enhances the droplet indentation. Numerical results were obtained for the capillary number Ca (varied in range of 0.1 - 1.0), the inner-to-outer droplet radius ratio R 21 (varied in range of 0.2 - 0.9), the droplet-to-tube radius ratio R 10 (varied in range of 0.2 - 0.9), the inner-to-outer interfacial tension coefficient ratio σ 21 (varied in range of 0.1 - 6.4), and the normalized depth of the constriction d/R (varied in range of 0.0 - 0.8). The results reveal that the most influencing factor is Ca, increasing its value leads to the increment of the maximum indentation at the trailing surface of the inner and outer droplets. The indentation is also increased with increasing the value of R 10and d/R. In contrast, increasing R 21 results in a decrease in the indentation at the trailing surface of the outer droplet. When increasing σ 21 , the indentation at the trailing surface of the inner one is quickly suppressed, while the outer droplet is minorly affected. We also point out the patterns of the trailing surface of the inner and outer droplets and their transitions from one to the other patterns in the diagrams based on these parameters.
Hydrodynamics and simulation of air–water homogeneous bubble column under elevated pressure
Tran, Bay V.,Nguyen, Dan D.,Ngo, Son I.,Lim, Youngx2010,Il,Kim, Bongjun,Lee, Dong Hyun,Go, Kangx2010,Seok,Nho, Namx2010,Sun American Institute of Chemical Engineers 2019 AIChE Journal Vol.65 No.10
<P><B>Abstract</B></P><P>A gas–liquid Eulerian computational fluid dynamics (CFD) model coupled with a population balance equation (PBE) was presented to investigate hydrodynamics of an air–water bubble column (1.8 m in height and 0.1 m in inner diameter) under elevated pressure in terms of pressure drop, gas holdup, mean bubble size, and bubble surface area. The CFD‐PBE model was modified with three pressure correction factors to predict both the total gas holdup and the mean bubble size in the homogeneous bubbly flow regime. The three correction factors were optimized compared to experimental data. Increasing the pressure led to increasing the density, reducing the bubble size, and increasing the gas holdup. The bubble size distribution moved toward a smaller bubble size, as the pressure increased. The modified CFD‐PBE model validated with experimental data and empirical models represented well hydrodynamics of the bubble column at <I>P</I> = 0.1, 1.5, and 3.5 MPa.</P>
Physciosporin suppresses the proliferation, motility and tumourigenesis of colorectal cancer cells
Tax15f,, x130,sa,Han, Jin,Park, So-Yeon,Yang, Yi,Zhou, Rui,Gamage, Chathurika D.B.,Van Nguyen, Tru,Lee, Ji-Yoon,Choi, Yong Jae,Yu, Young Hyun,Moon, Kyung-Sub,Kim, Kyung Keun,Ha, Hyung-Ho,Kim, Sang Elsevier 2019 Phytomedicine Vol.56 No.-
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Lichens, which represent symbiotic associations of fungi and algae, are potential sources of numerous natural products. Physciosporin (PHY) is a potent secondary metabolite found in lichens and was recently reported to inhibit the motility of lung cancer cells via novel mechanisms.</P> <P><B>Purpose</B></P> <P>The present study investigated the anticancer potential of PHY on colorectal cancer (CRC) cells.</P> <P><B>Methods</B></P> <P>PHY was isolated from lichen extract by preparative TLC. The effect of PHY on cell viability, motility and tumourigenicity was elucidated by MTT assay, hoechst staining, flow cytometric analysis, transwell invasion and migration assay, soft agar colony formation assay, Western blotting, qRT-PCR and PCR array <I>in vitro</I> as well as tumorigenicity study <I>in vivo</I>.</P> <P><B>Results</B></P> <P>PHY decreased the viability of various CRC cell lines (Caco2, CT26, DLD1, HCT116 and SW620). Moreover, PHY elicited cytotoxic effects by inducing apoptosis at toxic concentrations. At non-toxic concentrations, PHY dose-dependently suppressed the invasion, migration and colony formation of CRC cells. PHY inhibited the motility of CRC cells by suppressing epithelial-mesenchymal transition and downregulating actin-based motility markers. In addition, PHY downregulated β-catenin and its downstream target genes cyclin-D1 and c-Myc. Moreover, PHY modulated KAI1 C-terminal-interacting tetraspanin and KAI1 expression, and downregulated the downstream transcription factors c-jun and c-fos. Finally, PHY administration showed considerable bioavailability and effectively decreased the growth of CRC xenografts in mice without causing toxicity.</P> <P><B>Conclusion</B></P> <P>PHY suppresses the growth and motility of CRC cells via novel mechanisms.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture
Pax15f,ca, Anca M,Sloan, Steven A,Clarke, Laura E,Tian, Yuan,Makinson, Christopher D,Huber, Nina,Kim, Chul Hoon,Park, Jin-Young,O'Rourke, Nancy A,Nguyen, Khoa D,Smith, Stephen J,Huguenard, John R,Ge Nature Publishing Group 2015 NATURE METHODS Vol. No.
The human cerebral cortex develops through an elaborate succession of cellular events that, when disrupted, can lead to neuropsychiatric disease. The ability to reprogram somatic cells into pluripotent cells that can be differentiated in vitro provides a unique opportunity to study normal and abnormal corticogenesis. Here, we present a simple and reproducible 3D culture approach for generating a laminated cerebral cortex–like structure, named human cortical spheroids (hCSs), from pluripotent stem cells. hCSs contain neurons from both deep and superficial cortical layers and map transcriptionally to in vivo fetal development. These neurons are electrophysiologically mature, display spontaneous activity, are surrounded by nonreactive astrocytes and form functional synapses. Experiments in acute hCS slices demonstrate that cortical neurons participate in network activity and produce complex synaptic events. These 3D cultures should allow a detailed interrogation of human cortical development, function and disease, and may prove a versatile platform for generating other neuronal and glial subtypes in vitro.
Le, M. T.,Choi, H.,Choi, M.x2010,K.,Nguyen, D. T.,Kim, J.x2010,H.,Seo, H. G.,Cha, S.x2010,Y.,Seo, K.,Chun, T.,Schook, L. B.,Park, C. Munksgaard 2012 Tissue antigens Vol.80 No.6
<P><B>Abstract</B></P><P>We previously reported the development of genomic‐DNA‐based high‐resolution genotyping methods for SLA‐DQB1 and DRB1. Here, we report the successful typing of SLA‐DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA‐DQA using intronic sequence information of SLA‐DQA exon 2 that we had obtained from 12 animals with different SLA‐DQB1 genotypes. We expanded our typing to 76 selected animals with diverse DQB1 and DRB1 genotypes, 140 random animals from 7 pig breeds, and 3 wild boars. This resulted in the identification of 17 DQA alleles with 49 genotypes. Two new alleles were identified from wild boars. Combine with SLA‐DQB1, and DRB1 typing results, we identified 34 SLA class II haplotypes including 25 that were previously unreported.</P>
Pham, Locx2010,Duyen D.,Hayakawa, Kazuhide,Seo, Ji Hae,Nguyen, Minhx2010,Nguyet,Som, Angel T.,Lee, Brian J.,Guo, Shuzhen,Kim, Kyux2010,Won,Lo, Eng H.,Arai, Ken Wiley Subscription Services, Inc., A Wiley Company 2012 GLIA Vol.60 No.6
<P><B>Abstract</B></P><P>After stroke and brain injury, cortical gray matter recovery involves mechanisms of neurovascular matrix remodeling. In white matter, however, the mechanisms of recovery remain unclear. In this study, we demonstrate that oligodendrocytes secrete matrix metalloproteinase‐9 (MMP‐9), which accelerates the angiogenic response after white matter injury. In primary oligodendrocyte cultures, treatment with the proinflammatory cytokine interleukin‐1β (IL‐1β) induced an upregulation and secretion of MMP‐9. Conditioned media from IL‐1β‐stimulated oligodendrocytes significantly amplified matrigel tube formation in brain endothelial cells, indicating that MMP‐9 from oligodendrocytes can promote angiogenesis <I>in vitro</I>. Next, we asked whether similar signals and substrates operate after white matter injury <I>in vivo</I>. Focal white matter injury and demyelination was induced in mice via stereotactic injection of lysophosphatidylcholine into corpus callosum. Western blot analysis showed that IL‐1β expression was increased in damaged white matter. Immunostaining demonstrated MMP‐9 signals in myelin‐associated oligodendrocytic basic protein‐positive oligodendrocytes. Treatment with an IL‐1β‐neutralizing antibody suppressed the MMP‐9 response in oligodendrocytes. Finally, we confirmed that the broad spectrum MMP inhibitor GM6001 inhibited angiogenesis around the injury area in this white matter injury model. In gray matter, a neurovascular niche promotes cortical recovery after brain injury. Our study suggests that an analogous oligovascular niche may mediate recovery in white matter. © 2012 Wiley Periodicals, Inc.</P>