국내 분리 일본뇌염 바이러스 유전자에 대한 항체생성 연구(Ⅰ) : cloning of JEV viral genes

박찬,최우영,이호동,채수림,신은진,김인범,표재환,박근용,조해월 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Activated T Cells Secrete an Alternatively Spliced Form of Common γ-Chain that Inhibits Cytokine Signaling and Exacerbates Inflammation

Hong, C.,Luckey, Megan A.,Ligons, Davinna L.,Waickman, Adam T.,Park, J.Y.,Kim, Grace Y.,Keller, Hilary R.,Etzensperger, R.,Tai, X.,Lazarevic, V.,Feigenbaum, L.,Catalfamo, M.,Walsh, Scott T.R.,Park, J. Cell Press 2014 Immunity Vol.40 No.6

The common γ-chain (γc) plays a central role in signaling by IL-2 and other γc-dependent cytokines. Here we report that activated T cells produce an alternatively spliced form of γc mRNA that results in protein expression and secretion of the γc extracellular domain. The soluble form of γc (sγc) is present in serum and directly binds to IL-2Rβ and IL-7Rα proteins on T cells to inhibit cytokine signaling and promote inflammation. sγc suppressed IL-7 signaling to impair naive T cell survival during homeostasis and exacerbated Th17-cell-mediated inflammation by inhibiting IL-2 signaling upon T cell activation. Reciprocally, the severity of Th17-cell-mediated inflammatory diseases was markedly diminished in mice lacking sγc. Thus, sγc expression is a naturally occurring immunomodulator that regulates γc cytokine signaling and controls T cell activation and differentiation.

Interleukin-18, transforming growth factor-β, and vascular endothelial growth factor gene polymorphisms and susceptibility to primary glomerulonephritis

Choi, H.-J.,Cho, J.-H.,Kim, J.-C.,Seo, H.-J.,Hyun, S.-H.,Kim, G.-H.,Choi, J.-Y.,Choi, H.-J.,Ryu, H.-M.,Cho, J.-H.,Park, S.-H.,Kim, Y.-L.,Han, S.,Kim, C.-D. Blackwell Publishing Ltd 2010 Tissue antigens Vol.76 No.4

<P>Several studies have showed an association of gene polymorphisms with the development of glomerulonephritis (GN). We investigated the effects of gene polymorphisms on the development of GN by analyzing polymorphisms in the interleukin (IL)-18, transforming growth factor (TGF)-β, and vascular endothelial growth factor (VEGF) genes in Korean patients with primary GN. The study included 146 normal subjects (controls) and 100 patients diagnosed with primary GN by kidney biopsy. The gene polymorphisms A-607C and G-137C in <I>IL-18</I>, C-509T and T869C in <I>TGF-</I>β<I>1</I>, and C-2578A and C405G in <I>VEGF</I> were investigated in DNA extracted from peripheral blood. Significant differences were observed between the GN and control groups in the genotype and allele frequencies of A-607C <I>IL-18</I> and C405G <I>VEGF</I>. The frequencies of the <I>IL-18</I>−607CC genotype [<I>P</I> = 0.001, odds ratio (OR) = 2.473] and the <I>VEGF</I> 405GG genotype (<I>P</I> = 0.001, OR = 2.473) were significantly increased in the GN group. The combination of <I>IL-18</I>−607CC+ and <I>VEGF</I> 405GG+ genotypes had a higher risk for developing GN in comparison with the combination of <I>IL-18</I>−607CC− and <I>VEGF</I> 405GG− genotypes (<I>P</I> < 0.001, OR = 8.642). In the haplotype analysis of the <I>IL-18</I> gene, the CG haplotype was significantly more frequent in the GN group than the control group (61.5% <I>vs</I> 46.9%, <I>P</I> = 0.002). These results show that the −607CC genotype of the <I>IL-18</I> gene and the 405GG genotype of the <I>VEGF</I> gene are associated with susceptibility to and the development of primary GN.</P>

C1q/TNF-related protein-9 inhibits cytokine-induced vascular inflammation and leukocyte adhesiveness via AMP-activated protein kinase activation in endothelial cells

Jung, C.H.,Lee, M.J.,Kang, Y.M.,Lee, Y.L.,Seol, S.M.,Yoon, H.K.,Kang, S.W.,Lee, W.J.,Park, J.Y. North-Holland 2016 Molecular and cellular endocrinology Vol.419 No.-

<P>Although recent studies have reported cardioprotective effects of C1q/TNF-related protein 9 (CTRP9), the closet adiponectin paralog, its role on cytokine-induced endothelial inflammation is unknown. We investigated whether CTRP9 prevented inflammatory cytokine-induced nuclear factor-kappa B (NF-kappa B) activation and inhibited the expression of adhesion molecules and a chemokine in the vascular endothelial cell. We used human aortic endothelial cells (HAECs) to examine the effects of CTRP9 on NF-kappa B activation and the expression of NF-kappa B-mediated genes, including intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemoattractant protein-1 (MCP-1). Tumor necrosis factor alpha (TNF alpha) was used as a representative proinflammatory cytokine. In an adhesion assay using THP-1 cells, CTRP9 reduced TNF alpha-induced adhesion of monocytes to HAECs. Treatment with CTRP9 significantly decreased TNF alpha-induced activation of NF-kappa B, as well as the expression of ICAM-1, VCAM-1, and MCP-1. In addition, treatment with CTRP9 significantly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), the downstream target of AMPK. The inhibitory effect of CTRP9 on the expression of ICAM-1, VCAM-1, and MCP-1 and monocyte adhesion to HAECs was abolished after transfection with an AMPK alpha 1-specific siRNA. Our study is the first to demonstrate that CTRP9 attenuates cytokine-induced vascular inflammation in endothelial cells mediated by AMPK activation. (C) 2015 Elsevier Ireland Ltd. All rights reserved.</P>

Sensitivity of surface characteristics on the simulation of wind-blown-dust source in North America

Park, S.H.,Gong, S.L.,Gong, W.,Makar, P.A.,Moran, M.D.,Stroud, C.A.,Zhang, J. Pergamon Press ; Elsevier [distribution] 2009 Atmospheric environment Vol.43 No.19

Recently, a wind-blown-dust-emission module has been built based on a state-of-the-art wind erosion theory and evaluated in a regional air-quality model to simulate a North American dust storm episode in April 2001 (see Park, S.H., Gong, S.L., Zhao, T.L., Vet, R.J., Bouchet, V.S., Gong, W., Makar, P.A., Moran, M.D., Stroud, C., Zhang, J. 2007. Simulation of entrainment and transport of dust particles within North America in April 2001 (''Red Dust episode''). J. Geophys. Res. 112, D20209, doi:10.1029/2007JD008443). A satisfactorily detailed assessment of that module, however, was not possible because of a lack of information on some module inputs, especially soil moisture content. In this paper, the wind-blown-dust emission was evaluated for two additional dust storms using improved soil moisture inputs. The surface characteristics of the wind-blown-dust source areas in southwestern North America were also investigated, focusing on their implications for wind-blown-dust emissions. The improved soil moisture inputs enabled the sensitivity of other important surface characteristics, the soil grain size distribution and the land-cover, to dust emission to be investigated with more confidence. Simulations of the two 2003 dust storm episodes suggested that wind-blown-dust emissions from the desert areas in southwestern North America are dominated by emissions from dry playas covered with accumulated alluvial deposits whose particle size is much smaller than usual desert sands. As well, the source areas in the northwestern Texas region were indicated to be not desert but rather agricultural lands that were ''activated'' as a wind-blown-dust sources after harvest. This finding calls for revisions to the current wind-blown-dust-emission module, in which ''desert'' is designated to be the only land-cover category that can emit wind-blown dust.

Development and evaluation of triplex PCR for direct detection of mycobacteria in respiratory specimens

Park, H.,Kim, C.,Park, K.H.,Chang, C.L. Blackwell Science Ltd 2006 Journal of applied microbiology Vol.100 No.1

<P>Abstract</P><P>Aims: </P><P>To develop a method for the simultaneous detection of <I>Mycobacterium tuberculosis</I>, <I>Mycobacterium avium-intracellulare</I>complex (MAC), and other mycobacteria in clinical specimens using triplex PCR.</P><P>Methods and Results: </P><P>The target of amplification was the internal transcribed spacer region between the l6S and 23S rDNA genes. Twenty-two mycobacterial type strains, 118 <I>M. tuberculosis</I>, 87 other mycobacteria, 75 nonmycobacterial pathogens, 115 respiratory specimens from confirmed cases of tuberculous or other mycobacterial diseases, and sputa from 50 patients with nonmycobacterial respiratory diseases were tested. In <I>M. tuberculosis</I>, 322 bp pan-mycobacterial and 133 bp species-specific bands were observed. In MAC, the respective bands were 322 and 84 bp. The other mycobacteria showed single pan-mycobacterial bands of approx. 300–350 bp. Nonspecific amplicons were not found in any of the nonmycobacterial pathogens. In the tuberculosis specimens, 96·4% of smear-positive specimens and 70·2% of smear-negative specimens showed positive reactions. Specimens from two patients with MAC infection were MAC positive. Only 1 of 50 specimens from nonmycobacterial diseases was positive (2·0%).</P><P>Conclusion, Significance and Impact of the Study: </P><P>Triplex PCR enables accurate and rapid diagnosis of tuberculosis and probably is useful for the detection of MAC and other mycobacteria in respiratory specimens.</P>

Reduced water vapor transmission rates of low-temperature-processed and sol-gel-derived titanium oxide thin films on flexible substrates

Park, S.,Kim, L.H.,Jeong, Y.J.,Kim, K.,Park, M.,Baek, Y.,An, T.K.,Nam, S.,Jang, J.,Park, C.E. Elsevier Science 2016 Organic electronics Vol.36 No.-

<P>Sol-gel-derived, crack-free, and condensed TiOx thin films with improved barrier properties were successfully fabricated on polymeric substrates with a simple two-step heat treatment at low temperatures. To assess the barrier properties of the TiOx thin films, Ca corrosion tests were conducted and their water vapor transmission rates (WVTRs) were measured. We found that the two-step heat treatment (at 45 degrees C for 90 min and 110 degrees C for 60 min) produces a close-packed TiOx structure that substantially reduces the WVTRs of the coated polymeric substrates. The WVTRs of 86 nm thick TiOx thin films on polyethylene naphthalate (PEN) substrates at a relative humidity (RH) of 90% were found to be 0.133 g m(-2) day(-1) at 38 degrees C and 0.0387 g m(-2) day(-1) at 25 degrees C. In addition, the WVTR value of the TiOx thin films on PEN substrates are stable with respect to bending: it was found to increase by only similar to 13% after 100 repetitions of bending with a 20 mm radius. (C) 2016 Elsevier B.V. All rights reserved.</P>

Comparison of the Haas and the Oxford classifications for prediction of renal outcome in patients with IgA nephropathy

Park, K.S.,Han, S.H.,Kie, J.H.,Nam, K.H.,Lee, M.J.,Lim, B.J.,Kwon, Y.E.,Kim, Y.L.,An, S.Y.,Kim, C.H.,Doh, F.M.,Koo, H.M.,Oh, H.J.,Kang, S.W.,Choi, K.H.,Jeong, H.J.,Yoo, T.H. W. B. Saunders Co ; Centrum Philadelphia 2014 Human pathology Vol.45 No.2

Pathologic features can provide valuable information for determining prognosis in IgA nephropathy (IgAN). However, it is uncertain whether the Oxford classification, a new classification of IgAN, can predict renal outcome better than previous ones. We conducted a retrospective cohort study in 500 patients with biopsy-proven IgAN between January 2002 and December 2010 to compare the ability of the Haas and the Oxford classifications to predict renal outcome. Primary outcome was a doubling of the baseline serum creatinine concentration (D-SCr). During a mean follow-up of 68months, 52 (10.4%) and 35 (7.0%) developed D-SCr and end-stage renal disease, respectively. There were graded increases in the development of D-SCr in the higher Haas classes. In addition, the primary endpoint of D-SCr occurred more in patients with the Oxford M and T lesions than those without such lesions. In multivariate Cox regression analyses, the Haas class V (HR, 12.19; P=.002) and the Oxford T1 (hazard ratio [HR], 6.68; P<.001) and T2 (HR, 12.16; P<.001) lesions were independently associated with an increased risk of reaching D-SCr. Harrell's C index of each multivariate model with the Haas and the Oxford classification was 0.867 (P=.015) and 0.881 (P=.004), respectively. This was significantly higher than that of model with clinical factors only (C=0.819). However, there was no difference in C-statistics between the 2 models with the Haas and the Oxford classifications (P=.348). This study suggests that the Haas and the Oxford classifications are comparable in predicting progression of IgAN.

CTRP1 protects against diet-induced hyperglycemia by enhancing glycolysis and fatty acid oxidation

Han, S.,Park, J.S.,Lee, S.,Jeong, A.L.,Oh, K.S.,Ka, H.I.,Choi, H.J.,Son, W.C.,Lee, W.Y.,Oh, S.J.,Lim, J.S.,Lee, M.S.,Yang, Y. Butterworths ; Elsevier Science Ltd 2016 The Journal of nutritional biochemistry Vol.27 No.-

<P>Complement-C1q/tumor necrosis factor-alpha related protein 1 (CTRP1) is a 35-kDa glycoprotein that is secreted from various tissues. Although CTRP1 is highly increased in patients with type II diabetes and obesity, the metabolic roles of CTRP1 remain largely unknown. To unveil the physiological roles of CTRP1 in vivo, CTRP1 transgenic (TG) mice were challenged by a high-fat diet (HFD) and a high-sucrose drink (HS). Homeostatic model assessment-estimated insulin resistance values were decreased in HFD- or HS-fed CTRP1 TG mice compared with wild-type control mice. In this context, CTRP1 stimulated glucose uptake through the glucose transporter GLUT4 translocation to the plasma membrane and also increased glucose consumption by stimulating glycolysis. To analyze the roles of CTRP1 in lipid metabolism, acetyl-CoA carboxylase (ACC) and hormone-sensitive lipase levels were determined in CTRP1 TG mice, and the effect of CTRP1 on fatty acid oxidation was assessed in C2C12 myotubes. CTRP1 was found to inhibit ACC by phosphorylation and to stimulate fatty acid oxidation in C2C12 myotubes. Taken together, CTRP1 performs active catabolic roles in vivo. Therefore, CTRP1 seems to perform a defensive function against nutritional challenges. (C) 2015 Elsevier Inc. All rights reserved.</P>

서울시 지하철 구내의 공기중 분진 농도에 관한 연구

백남원(N.W. Paik),박두용(D.Y. Park),장익선(I.S. Chang),신용철(Y.C. Shin),이정인(J. l. Lee) 한국환경보건학회 1988 한국환경보건학회지 Vol.14 No.2

Airborne dust and asbestos fiber concentrations were determined in subway stations located in Seoul area. Two stations, such as Eulchiro 4.Ka Station of Une #2, constructed during a period of 1980-1984 and Hyehwa Station of Une #4, opened in 1985, were selected. The results of the study are as follows. 1. Daily tirne.weighted average (1W A) concentrations of airborne dusts from 07:00 to 20:00 hours in Line #2 and μne #4 were 0.43:t0.08 mg/m3 and 0.37:t0.12 mg/m3 , respectively. Thus, the dust levels in Líne #2 were significant1y higher than the levels in Line #4( p < 0.05). 2. Dust levels in the morning (07:00-11:00 hours), noon (1 1:00-16:00 hours) and in the evening (1 6:00-20:00 hours) in Líne #2 were 0.47:t0.17 mgjm3 , 0.37:t0.08 mg/m3 , and 0.46土0.07 mg/m3 respectively. Thus, dust levels in the morning and evening (i.e., during rush hours) were significantly higher than levels in the noon ( p < 0.02). However, there was no such difference in dust levels by time in Líne #4. 3. Airborne total dust concentrations were well below the occupational health standard of 10 mg/m3 , however, the levels were exceeding the ambient air standard recommended by the Korean Environment Administration. 4. All of airborne asbestos fiber concentrations were equal to or below 0.005 fibers/cc. The levels are within both occupational health standards and U .S. EPA criterìa. 5. A consideration should be given to the improvements of c1eaning methods (such as use of vacuum c1eaning instead of brushing) and the existing ventilation (such as more aìr change and filtration of supply air) for a reduction of dust levels. 6. It is recommended that routine monitoring of aìrborne dusts and asbestos fibers be conducted because more dusts and asbestos fibers can be produced in the aìr due to the deterioration of facilities by age and water damage in future.