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( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.
Dynamic Guaranteed Cost Compression for Time Series Big Data
Miao Bei-bei,Jin Xue-bo 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.4
Most time series big data is with noise and uncertain. To abstract the key information effectively and quickly, the estimation is one of the feasible methods for the uncertain big data. The Kalman filter with adaptive method by part of samples can give the high dimensional characteristics, reduce the computing cost and data uncertainty, but encounter the irregular estimation. The number of sample and the performance of the abstracted information have the tradeoff, which means we can use the suitable number of sample to abstract the key information of the series data. This paper discusses how to find the suitable sampling points for the time series data and the simulations show that the key dynamic information of time series big data can be guaranteed with the compression amount number of sample data.
Syntheses and Characterizations of Two New Cadmium Complexes Based on Oxalate
Ming-San Miao,Bo-Lin Cheng,Zhen Liang,Huai-Xia Yang,Xiang-Ru Meng 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.9
Two new Cd(II) complexes, {[Cd(COO)2]2·2H2O}n (1) and {[CdCl(COO)2(Hbmi)]·H2O} n (2), were obtained through the reactions of oxalic acid (H2ox) with CdCl2 ·2.5H2O in the presence of 1-[(benzoimidazol-yl)methyl]-1H-tetrazole (bmt) or 1-[(benzoimidazol-yl)methyl]-1H-imidazol (bmi). Single-crystal X-ray diffraction shows that complex 1 has a 3-D structure with the topological notation of (48 · 62)(46 · 66 · 83)(42 · 84), in which the oxalate displays two kinds of coordination modes: formation of the layers (μ6-ox) and linking the layers (μ4-ox). In complex 2, oxalates bridge Cd(II) ions, forming a 1-D chain, and (Hbmi)+ cations coordinate to the Cd(II) ions in monodentate mode and hang at two sides of the main chain. This indicates that subtle modification of the N-donor ligands can result in complexes with different compositions and architectures. Moreover, their IR spectra, PXRD (powder X-ray diffraction) patterns, thermogravimetric analyses, and fluorescence properties are also investigated.
Dynamic Characteristics of Saturated Soft Clays under Cyclic Loading in Drained Condition
Yong-hong Miao,Ruo-Yu Sheng,Jie Yin,Fan-Bo Zhou,Jian-fei Lu 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.2
This study presents an experimental investigation on the effects of cyclic loading waveform and cyclic stress ratio (CSR) on dynamic behaviors through laboratory tests on a source of soft clay under cyclic loading in drained condition. Test results showed that under different cyclic loading waveforms at a given frequency (f = 1 Hz) and CSR = 0.6, the axial strain increases significantly at a relatively lower number of cycles (N) and then became stable, while the axial strain consistently decreases with the increasing N and tend to be stable. At a given N, specimen under sine wave cyclic loads shows a higher axial strain rate than that under triangle wave or square cyclic loads. Test results also indicated that at different CSRs, the axial strain increases rapidly at a relatively lower N and then gradually tends to be stable with the increasing N at lower CSR yet there is no stable trend for specimens at higher CSR, which indicates there is a critical value of CSR. It is recommended to apply the cyclic loading in sine waveform with CSR close to and less than the critical value to get a better treatment effect.
Ling‑Hong Miao,Wen‑Jing Pan,Yan Lin,Bo Liu,Ming‑Chun Ren,Qun‑Lan Zhou,Xian‑Ping Ge 한국유전학회 2017 Genes & Genomics Vol.39 No.12
The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.