RNA sequencing reveals that Prx II gene knockout can down-regulate the allograft rejection of dermal mesenchymal stem cells

Han Ying-Hao,Mao Ying-Ying,Yu Nan-Nan,Jin Mei-Hua,Jin Ying-Hua,Wang Ai-Guo,Zhang Yong-Qing,Shen Gui-Nan,Cui Yu-Dong,Yu Li-Yun,Lee Dong-Seok,Jo Yu-Jin,Sun Hu-Nan,Kwon Jeongwoo,권태호 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3

In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p ≤ 0.05) between the PrxII+/+ (WT) and PrxII−/− sample groups. When highly regulated genes were categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII−/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII−/− vs. PrxII+/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.

Anti-inflammatory effect of hispidin on LPS induced macrophage inflammation through MAPK and JAK1/STAT3 signaling pathways

Han Ying-Hao,Chen Dong-Qin,Jin Mei-Hua,Jin Ying-Hua,Li Jing,Shen Gui-Nan,Li Wei-Long,Gong Yi-Xi,Mao Ying-Ying,Xie Dan-Ping,Lee Dong-Seok,Yu Li-Yun,Kim Sun-Uk,김지수,권태호,Cui Yu-Dong,Sun Hu-Nan 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3

Severe inflammatory reactions caused by macrophage activation can trigger a systemic immune response. In the present study, we observed the anti-inflammatory properties of hispidin on LPS induced RAW264.7 macrophage cells. Our results showed that hispidin treatment significantly reduced the production of cellular NO, IL-6 and reactive oxygen species (ROS) while has not inhibitory effect on TNF-α productions. Excitingly, hispidin treatment retains the phagocytosis ability of macrophages which enabling them to perform the function of removing foreign invaders. Signaling studies showed, hispidin treatment dramatic suppressed the LPS induced mitogen activated protein kinases (MAPK) and JAK/STAT activations. In conclusion, our findings suggest that hispidin may be a new therapeutic target for clinical treatment of macrophages-mediated inflammatory responses.

Extraction of optical constants using multiple reflections in the terahertz emitter-sample hybrid structure.

Han, J W,Hamh, S Y,Kim, T H,Lee, K S,Yu, Nan Ei,Ko, Do-Kyeong,Lee, J S Optical Society of America 2014 Optics letters Vol.39 No.19

<P>To extract optical constants of nontransmitted samples in the terahertz (THz) spectral region, we employ a THz emitter-sample hybrid structure where THz pulses are generated at the emitter surface and multiply reflected at the interface between the THz emitter and the sample. Since each THz electric field profile appears well separated in a time domain, we could obtain the amplitude and phase spectra for each pulse from the Fourier transform, and determine the optical constants of the sample numerically based on the Fresnel equations. We applied this technique for doped semiconductors, and found that obtained optical constants are in good agreement with the values determined by using other conventional THz techniques.</P>

Highly directional waveguide grating antenna for optical phased array

Han, Kyunghun,Yurlov, Victor,Yu, Nan Ei ELSEVIER 2018 CURRENT APPLIED PHYSICS Vol.18 No.7

<P>A highly directional waveguide grating antenna by patterning top cladding has been proposed. Spatial separation of the grating structure from the waveguide reduces the strength of perturbation to achieve a millimeter-scale emission. The optimized grating structure offers above 70% directionality with 800 nm waveguide width. The vertical field-of-view of 15 degrees is achieved with 100 nm wavelength bandwidth. Thanks to the its simple structure and large critical dimension, the proposed structure is suitable for manufacturing in CMOS foundries with DUV lithography.</P>

Speckle noise reduction on a laser projection display via a broadband green light source

Yu, Nan Ei,Choi, Ju Won,Kang, Heejong,Ko, Do-Kyeong,Fu, Shih-Hao,Liou, Jiun-Wei,Kung, Andy H.,Choi, Hee Joo,Kim, Byoung Joo,Cha, Myoungsik,Peng, Lung-Han The Optical Society 2014 Optics express Vol.22 No.3

The Protective Role of TLR3 and TLR9 Ligands in Human Pharyngeal Epithelial Cells Infected with Influenza A Virus

Han, Yan,Bo, Zhi-Jian,Xu, Ming-Yu,Sun, Nan,Liu, Dan-Hong The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.3

In this study we aim to extensively investigate the anti-influenza virus immune responses in human pharyngeal epithelial cell line (Hep-2) and evaluate the protective role of Toll-like receptor (TLR) ligands in seasonal influenza A H1N1 (sH1N1) infections in vitro. We first investigated the expression of the TLRs and cytokines genes in resting and sH1N1 infected Hep-2 cells. Clear expressions of TLR3, TLR9, interleukin (IL)-6, tumour necrosis factor (TNF)-${\alpha}$ and interferon (IFN)-${\beta}$ were detected in resting Hep-2 cells. After sH1N1 infection, a ten-fold of TLR3 and TLR9 were elicited. Concomitant with the TLRs activation, transcriptional expression of IL-6, TNF-${\alpha}$ and IFN-${\beta}$ were significantly induced in sH1N1-infected cells. Pre-treatment of cells with poly I:C (an analog of viral double-stranded RNA) and CpG-ODN (a CpG-motif containing oligodeoxydinucleotide) resulted in a strong reduction of viral and cytokines mRNA expression. The results presented indicated the innate immune response activation in Hep-2 cells and affirm the antiviral role of Poly I:C and CpG-ODN in the protection against seasonal influenza A viruses.

DEVELOPMENT OF CHINA LIGHT-DUTY VEHICLE TEST CYCLE

Yu Liu,Zhi Xin Wu,Hua Zhou,Han Zheng Nan Yu,Xiao Pan An,Jing Yuan Li,Meng Liang Li 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.5

Driving cycles provide a basis for vehicle development and calibration and also serves as the foundation for energy consumption and emissions certification of vehicles. This paper presents the China Light-Duty Vehicle Test Cycle (CLTC) developed by the China Automotive Technology & Research Center (CATARC). First, the important steps and technical routes toward the CLTC development process are summarized. Second, the specific CLTC development process is presented in detail, including the data acquisition and data analysis procedures, weighting factor development and driving cycle construction. Then, the main driving characteristics of the New European Driving Cycle (NEDC), the Worldwide Harmonized Light-Duty Vehicles Test Cycle (WLTC), the Federal Test Procedure (FTP-75), the CLTC and the actual collected data are compared. The CLTC has low average speed, a high idle speed ratio and more frequent acceleration and deceleration characteristics. Finally, 70 vehicles are t ested based on the NEDC, WLTC, and CLTC according to their legislative procedures in the vehicle emission laboratories of the CATARC and the manufacturers. The results show that the CLTC’s fuel consumption is much higher than that of the NEDC and WLTC, and CLTC can effectively reflect the actual fuel consumption of users.

차기 교육과정을 대비한 고등학교 가정교과(군) 선택과목의 구조화

유난숙(Yu, Nan Sook),백민경(Baek, Min Kyung),주수언(Ju, Sueun),한주(Han, Ju),박미정(Park, Mi Jeong) 한국가정과교육학회 2021 한국가정과교육학회지 Vol.33 No.1

본 연구는 대학의 가정 관련 전공 개설 현황과 중등학교 가정과교육과정에 요구되는 변화를 분야별로 살펴보고, 차기 고등학교교육과정에서 편성할 수 있는 가정교과(군)의 선택과목을 구조화하는 데 목적이 있다. 이를 위해 관련 문헌과 자료를 분석하고,가정교과 전문가를 대상으로 설문 조사와 FGI를 실시하였으며, 연구 결과는 다음과 같다. 첫째, 가정교과는 자연계열의 생활과학영역뿐만 아니라 사회계열, 교육계열, 공학계열, 예체능계열 등과 연관성이 높았다. 2019학년도 가정교과와 관련된 학과수는전문대학교 1,405개, 4년제 대학교 961개였으며, 총 입학생수를 기준으로 가정교과 관련 학과 입학생수는 전문대학교는 약12%, 4년제 대학교는 약 7.3%를 차지하여 고등학교 가정교과(군) 선택과목을 개설할 기반이 충분함을 확인하였다. 둘째, 중등학교가정과교육과정에서는 분야별로 달라진 식⋅의⋅주⋅가족⋅소비생활 패턴과 삶의 가치관에 따라 문화, 관계, 자립, 지속가능성등의 개념을 강조하였으며, 단순 지식적인 측면의 내용은 줄이고, 깊이 있고 고차원적인 사고와 문화를 향유할 수 있는 방식으로가정교과 내용이 구성되기를 희망하였다. 이러한 요구는 가정교과(군)의 선택과목을 다양화, 전문화하여 구조화함으로써 반영할수 있을 것이다. 셋째, 고등학교 가정교과(군) 선택과목을 아동⋅가족, 식생활, 의생활, 주생활, 소비⋅가정관리, 가정교과 통합분야에서 총 18개의 선택과목명과 과목 개요를 구조화하였다. 본 연구 결과는 차기 가정과교육과정 편성을 위한 기초 자료를제공하고, 고등학생들에게 가정교과(군)의 과목 선택권을 확대함으로써 고교학점제의 안착에도 기여할 것이다. The purposes of this study were to examine the current status of the establishment of home economics-related departmentsin colleges and universities and the changes required in the home economics curriculum of secondary schools, and to structurethe elective courses of home economics subject(group) that can be organized in the next high school curriculum. To achievethese purposes, related literature and data were analyzed, and a questionnaire survey and FGI were conducted by home economicsexperts. The research results are as follows. First, home economics was considered to be highly related not only to the humanecology but also to social sciences, education, engineering, and arts and physical education. The numbers of technical collegesand 4-year universities with departments related to home economics were 1,405 and 961 respectively in 2019. Therefore, itwas confirmed that there is a sufficient basis for opening home economics subject(group) elective courses in high school. Second,in the secondary school home economics curriculum, the concepts of culture, relations, independence, and sustainability wereemphasized based on the changing life patterns and values. It was proposed that the contents of the home economics coursewould be structured in a way that allows deep and high-level thinking and helps students to enjoy culture. This demand canbe implemented by diversifying, specializing, and structuring the elective courses of the home economics subject(group). Third,a total of 18 elective subjects and subject outlines were structured in the fields of child/family, food/nutrition, clothing, housing,consumption/family management, and home economics integration. This study results will contribute to the establishment ofthe high school credit system by providing basic information for organizing the next home economics curriculum, and expandingthe options for home economics subject(group) to high school students

The Protective Role of TLR3 and TLR9 Ligands in Human Pharyngeal Epithelial Cells Infected with Influenza A Virus

Yan Han,Ming-yu Xu,Nan Sun,Dan-hong Liu,Zhi-jian Bo 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.3

In this study we aim to extensively investigate the anti-influenza virus immune responses in humanpharyngeal epithelial cell line (Hep-2) and evaluate the protective role of Toll-like receptor (TLR)ligands in seasonal influenza A H1N1 (sH1N1) infections in vitro. We first investigated the expressionof the TLRs and cytokines genes in resting and sH1N1 infected Hep-2 cells. Clear expressions of TLR3,TLR9, interleukin (IL)-6, tumour necrosis factor (TNF)-α and interferon (IFN)-β were detected inresting Hep-2 cells. After sH1N1 infection, a ten-fold of TLR3 and TLR9 were elicited. Concomitantwith the TLRs activation, transcriptional expression of IL-6, TNF-α and IFN-β were significantlyinduced in sH1N1-infected cells. Pre-treatment of cells with poly I:C (an analog of viral double-strandedRNA) and CpG-ODN (a CpG-motif containing oligodeoxydinucleotide) resulted in a strong reductionof viral and cytokines mRNA expression. The results presented indicated the innate immune responseactivation in Hep-2 cells and affirm the antiviral role of Poly I:C and CpG-ODN in the protectionagainst seasonal influenza A viruses.

Risk of the Development of Diabetes and Cardiovascular Disease in Metabolically Healthy Obese People : The Korean Genome and Epidemiology Study

Kim, Nan Hee,Seo, Ji A,Cho, Hyunjoo,Seo, Ji Hye,Yu, Ji Hee,Yoo, Hye Jin,Kim, Sin Gon,Choi, Kyung Mook,Baik, Sei Hyun,Choi, Dong Seop,Shin, Chol,Cho, Nam Han Williams & Wilkins Co 2016 Medicine Vol.95 No.15

<P><B>Abstract</B></P><P>The reported effects of a metabolically healthy obese (MHO) phenotype on diabetes and cardiovascular disease (CVD) risk are contradictory. Within the context of a population-based cohort study, we aimed to investigate the long-term risk of an MHO status for the development of diabetes and CVD, and whether consistency of this phenotype or age affected cardiometabolic outcomes.</P><P>We recruited 7588 subjects without diabetes or CVD, aged 40 to 69 years at baseline examination, from the Korean Genome and Epidemiology Study, and followed-up these subjects for 10 years biennially. Participants were divided into 4 groups based on the body mass index and the presence of metabolic syndrome: metabolically healthy normal weight (MHNW), MHO, metabolically unhealthy normal weight (MUNW), and metabolically unhealthy obese (MUO). We defined persistent phenotypes if subjects maintained the same phenotype at every visit from baseline to their last visit. Incident diabetes and CVD morbidity or mortality were identified during 10 years of follow-up.</P><P>Compared to MHNW controls, MUNW and MUO groups had increased risk for development of diabetes (hazard ratio [HR] 3.0 [95% CI: 2.5–3.6], and 4.0 [3.4–4.7], respectively) and CVD (HR 1.6 [1.3–2.0], and 1.9 [1.5–2.4], respectively). However, the MHO group showed only a marginal increase in risk for diabetes and CVD (HR 1.2 [0.99–1.6], 1.4 [0.99–1.8], respectively). The impact of MHO on the development of diabetes was more prominent in younger individuals (HR 1.9 [1.2–3.1] vs 1.1 [0.8–1.4], <45 years vs ≥45 years at baseline). Only 15.8% of MHO subjects maintained the MHO phenotype at every visit from baseline to the 5th biennial examination (persistent MHO). In subjects with persistent MHO, the risk for diabetes and CVD was significantly higher than those with persistent MHNW (1.9 [1.2–3.1], 2.1 [1.2–3.7], respectively).</P><P>MHO phenotype, even if maintained for a long time, was associated with a significantly higher risk for the development of diabetes and CVD in Korean subjects.</P>