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An Xueying,Wang Rongliang,Lv Zhongyang,Wu Wenshu,Sun Ziying,Wu Rui,Yan Wenjin,Jiang Qing,Xu Xingquan 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-
Osteoarthritis (OA) is the most common form of arthritis. However, the exact pathogenesis remains unclear. Emerging evidence shows that N6-methyladenosine (m6A) modification may have an important role in OA pathogenesis. This study aimed to investigate the role of m6A writers and the underlying mechanisms in osteoarthritic cartilage. Among m6A methyltransferases, Wilms tumor 1-associated protein (WTAP) expression most significantly differed in clinical osteoarthritic cartilage. WTAP regulated extracellular matrix (ECM) degradation, inflammation and antioxidation in human chondrocytes. Mechanistically, the m6A modification and relative downstream targets in osteoarthritic cartilage were assessed by methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing, which indicated that the expression of frizzled-related protein (FRZB), a secreted Wnt antagonist, was abnormally decreased and accompanied by high m6A modification in osteoarthritic cartilage. In vitro dysregulated WTAP had positive effects on β-catenin expression by targeting FRZB, which finally contributed to the cartilage injury phenotype in chondrocytes. Intra-articular injection of adeno-associated virus-WTAP alleviated OA progression in a mouse model, while this protective effect could be reversed by the application of a Wnt/β-catenin activator. In summary, this study revealed that WTAP-dependent RNA m6A modification contributed to Wnt/β-catenin pathway activation and OA progression through post-transcriptional regulation of FRZB mRNA, thus providing a potentially effective therapeutic strategy for OA treatment.
Xueying Chai,Zhilu Ai,Jun Liu,Ting Guo,Jingyan Wu,Jie Bai,Qinlu Lin 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.7
The effects of the secondary metabolite biosynthesison the metabolism and morphology of the Monascuspurpureus were investigated in this study. Hypha andseptum length became longer after deletion of genes pigRand pksCT in M. purpureus LQ-6 by Agrobacteriumtumefaciens-mediated transformation technology, highlybranched hyphae, much smaller and freely dispersedmycelial pellets were observed in M. purpureus. Comparedwith that in the wild-type, the level of intracellular NADHand NADPH was almost constant in M. purpureus DpigRat 4 days, but the NADH and NADPH levels decreased by1.58-fold and 3.71-fold in M. purpureus DpksCT. Thepresent study can not only provide a kind of strategy toimprove the Monascus pigments production, but also providetheoretical support for the further study of relationshipbetween the secondary metabolites, metabolism and morphologicalchange.
Xueying Tao,Jong Yul Roh,Jae Young Choi,Yong Wang,Qin Liu,Hee Jin Shim,Yeon Ho Je 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
The baculovirus Autographa californica nucleopolyhedrovirus (AcMNPV), a large circular double-stranded DNA virus whose genome encodes at least 155 open reading frames (ORFs), is highly pathogenic to a number of lepidopteran insects and widely used to transduce various cells for exogenous gene expression. Although many genes of AcMNPV have been identified, the genome-wide study related to viral replication has not been well announced. In this study, to elucidate DNA replication cascade of AcMNPV, we firstly developed a novel baculovirus genome that can be maintained in Escherichia coli as a plasmid and can infect susceptible lepidopteran insect cells. This genome, named bAc-MK, contains a mini-F replicon and a kanamycin resistance marker. Using a convenient Tn7 transposon-based system, pPCS-S, which contains an ampicillin resistance gene, ORF knock-out mutants were generated by random insertion into bAc-MK genome. These mutants will be suffered DNA microarray to elucidate AcMNPV replication cascade.
Xueying Chen,Byenghee chang 한국콘텐츠학회 2020 International Journal of Contents Vol.16 No.2
In this study, an investigation was conducted into the influencing factors for the learning motivation of players in the game, including experience, vicarious experience, the need of achievement, the need of power, and mastery motivation. Then, a discussion was conducted regarding the role played by learning motivation, learning performance, and satisfaction with continuous use. A survey was conducted with 519 players, most at the intermediate gaming level in 〈King of Glory〉. As demonstrated by the results of this study, experience, vicarious experience, the need of power, and the mastery of motivation have significant positive association with the players’ motivation of learning the game. Learning performance and satisfaction have a positive impact on the continuity of use. Additionally, the correlation between the need of achievement and learning motivation is insignificant. Overall, the research results confirm the significance of the social-cognitive theory relative to the learning motivation. Players began to transform, satisfied with their achievements in the game, as well as gradually evolving toward self-improvement to achieve satisfaction. It offers a new explanation and crucial reference for mastering the gaming trend among the contemporary players.
Xueying Li,He Huang,Bing Xu,Hongqiang Guo,Yingcheng Lin,Sheng Ye,Jiqun Yi,Wenyu Li,Xiangyuan Wu,Wei Wang,Hongyu Zhang,Derong Xie,Jiewen Peng,Yabing Cao,Xingxiang Pu,Chengcheng Guo,Huangming Hong,Zhao 대한암학회 2019 Cancer Research and Treatment Vol.51 No.3
Purpose Rituximab with cyclophosphamide, doxorubicin, vincristine, and prednisone administered every 3 weeks (R-CHOP-21) is the standard care for diffuse large B-cell lymphoma (DLBCL). It is unknown whether the dose-dense R-CHOP (R-CHOP-14) could improve the outcome of the disease in Asian population. Materials and Methods Newly diagnosed DLBCL patients were centrally, randomly assigned (1:1) to receive R-CHOP- 14 or R-CHOP-21. R-CHOP-14 was administered every 2 weeks, and R-CHOP-21 was administered every 3 weeks. Primary end point was disease-free survival (DFS). Secondary end points included overall survival (OS), progression-free survival (PFS), response rate and toxicities. Results Seven hundred and two patients were randomly assigned to receive R-CHOP-14 (n=349) or R-CHOP-21 (n=353). With a median follow-up of 45.6 months, the two groups did not differ significantly in 3-year DFS (79.6% for R-CHOP-14 vs. 83.2% for R-CHOP-21, p=0.311), 3-year OS (77.5% for R-CHOP-14 vs. 77.6% for R-CHOP-21, p=0.903), or 3-year PFS (63.2% for R-CHOP-14 vs. 66.1% for R-CHOP-21, p=0.447). Patients with an International Prognostic Index (IPI) score ! 2 had a poorer prognosis compared to those with an IPI score < 2. Grade 3/4 hematologic and non-hematologic toxicities were manageable and similar between R-CHOP-14 and R-CHOP-21. Conclusion R-CHOP-14 did not improve the outcome of DLBCL compared to R-CHOP-21 in Asian population. With manageable and similar toxicities, both of the two regimens were suitable for Asian DLBCL patients. For high-risk patients with IPI ! 2, new combination regimens based on R-CHOP deserve further investigation to improve efficacy.
Xueying Tian 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.2
The economy in Suzhou, China is in the late stages of its industrialisation, during which, the completeness of non-profit organizations (NPOs) has been an important indicator for measuring the all-round socio-economic development of Suzhou. Currently, the NPOs of Suzhou make up for deficiencies in the government and the market, and thus make a positive contribution to the political and economic development of Suzhou. However, it is easy to find that NPOs still suffer disadvantages such as slow development, rarity, and low capacity: these inhibit their active benefits. This research investigated the classification and development status of the NPOs in Suzhou; on this basis, it analyzed the main problems facing them. Moreover, aiming at these problems, countermeasures and suggestions were proposed so as to allow them to play a more important in the socio-economic system of Suzhou.
Adsorption of dyes from water by Prunella vulgaris stem and subsequent fungal decolorization
Xueying Zhang,Jun Zhou,Yuben Fan,Jiayang Liu 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.9
The residue of herbaceous Prunella vulgaris stem (PVS) was evaluated as a potential adsorbent for dye removal, followed by fungal cultivation to achieve dye degradation on solid waste. PVS was analyzed in terms of nutritional composition such as fiber, ash, protein, and fat, which not only played a role in dye adsorption but also provided solid matrix for fungal growth. Five dyes, namely, crystal violet (CV), methylene blue (MB), reactive black 5 (RB), indigo carmine (IC), and direct red 80 (DR), were tested as adsorbates but only CV and MB were effectively adsorbed. Effect of sorbent dose, contact time, dye concentration, and NaCl on adsorption was investigated individually. Langmuir model was suitable for fitting MB adsorption, while adsorption of CV adopted the Freundlich model. The adsorption capacity was calculated to be 625 mg/g for CV and 303 mg/g for MB, respectively. The adsorption process of both dyes was spontaneous and endothermic, and the adsorption followed pseudo 2nd order kinetic model and film diffusion model. The dyed PVS was finally cultivated with fungus Pycnoporus sp., wherein efficient dye decolorization was attained under solid state fermentation. As such, PVS coupled with subsequent fungal degradation might serve as novel alternative for dye effluent treatment.