도토리 추출물의 3T3-L1 세포 분화억제 효과

김지연,이진,이창원,김애정,Kim, Ji-Yeon,Lee, Jin,Lee, Chang-Won,Kim, Ae-Jung 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

본 연구에서는 만성질환의 주요 위험인자인 비만을 예방하기 위한 식품소재로서 3T3-L1세포를 이용하여 탈피한 도토리의 항비만 효과를 알아보고자 하였다. 3T3-L1 세포에서 생존율(MTT assay)을 측정한 결과, AE와 AW 시료 모두 $500{\mu}g/mL$ 농도에서는 다소 생존율의 감소를 보여, $300{\mu}g/mL$를 최종 농도로 정하였다. 3T3-L1 세포의 지질축적 억제 효과를 측정한 결과, 농도 $100{\mu}g/mL$로 처리하였을 때 두 시료 모두 지질축적량의 증가를 보였으나, $200{\mu}g/mL$ 처리농도에서 AE 시료는 82%로, AW 시료는 74%로 감소되다가 $300 {\mu}g/mL$ 농도에서는 두 시료 모두 약 53% 수준까지 지질축적이 억제되었다. 3T3-L1 세포에서 중성지방 억제 효과를 확인한 결과, AE 시료의 경우 $200{\mu}g/mL$ 농도에서 11%의 감소율, $300{\mu}g/mL$ 농도에서 42% 수준의 감소율을 보였다. AW 시료도 $200{\mu}g/mL$ 농도에서 5%의 감소율과 $300{\mu}g/mL$에서 41%의 감소율을 보였다. 3T3-L1 세포의 ROS 생성량을 측정한 결과, 시료 농도 $200{\mu}g/mL$에서 AE는 42%, AW는 33%로 $300{\mu}g/mL$에서는 AE는 58%, AW는 52%로 ROS 생성량의 억제를 보였다. 3T3-L1 세포에서 mRNA 발현에 미치는 영향을 대조군과 비교하였을 때 두 시료(AE와 AW) 모두 $300{\mu}g/mL$ 농도에서 $PPAR-{\gamma}$은 54%와 38%, aP2는 40%와 18% 수준의 발현을 억제시키는 것으로 나타났다. 이상의 결과로 볼 때 탈피한 도토리는 3T3-L1 세포의 분화를 억제함으로써 새로운 항비만 소재로의 가능성이 있는 것으로 판단된다. This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and $500 {\mu}g/mL$) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up $300 {\mu}g/mL$ of extracts, but was suppressed by level $500{\mu}g/mL$ of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations ($300{\mu}g/mL$), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, $PPAR-{\gamma}$ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.

Gerbera(Gerbera hybrida Hort)의 형태적 특징과 유전적 유연관계 분석

김현애,임현희,양원진,이재헌,이병영,이용문,권오창 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1

This study was conducted to analyze the morphological characteristics and genetic relationships among 24 species of Gerbera. Most of flower colors were pink. The numerical order of flower color was pink, orange, red, double-colored, and milk-white. Majority of flower types were single or semidouble flowers. A few species were double flowers. Flower diameters were from 7cm to 12cm, showed significant differences compared to other characteristics. Flower stalks were ranged from 55cm to 65cm. Only one species was the shortest as 55cm. The others were similar size as about 65cm. Main annual production yields were between 190 and 400 blossoms. Fifty seven reproducible polymorphic bands from eighty primers were used for analyses of genetic similarity. The genetic similarity of 24 collected Gerberas was largely classified into five groups. The average similarity coefficient was 0.72 ranged from 0.50 to 0.90. The highest similarity coefficient was shown between 'Sardana' with red/white flower color and double flower type, and 'Tamara' with orange flower color and double flower type as 0.90.

뽕잎추출액코팅농도에 따른 뽕잎쌀밥의 품질에 관한 연구

김애정,노정옥,우경자,최원석 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.5

The purpose of this study was to assess the optimum coating ratio for rice, using various ratios of mulberry leaves extract, 1.0, 1.5, and 2.0%, and to determine the optimum ratio of added water, in proportion to the total weight of mulberry rice. The moisture content of the soaked rice, and the optimum water uptake rate, moisture content of the cooked rice, as well as its blue and color values, mechanical characteristics, internal structure and sensory evaluation, were analyzed. The statistical data analyses were completed using the SAS program. The results are summarized as follows: The moisture content of mulberry rice was less than that of raw rice. The average optimum water uptake of the soaked mulberry rice at the different water temperatures, 10, 20 and 30℃, was 20% of the total weight of the raw mulberry rice. As for the results of the sensory evaluation; 140% water, in proportion to the total weight of raw mulberry rice, was judged to be the optimum. The average moisture content of the cooked mulberry rice was 45~50%, but there was no significant difference in the various coating ratios. The blue value of the cooked mulberry rice awas highest on the first day of cooking. The L- and a-values decreased with increasing coating ratio, but the b-value increased under the same conditions. As for the mechanical characteristics,; the adhesiveness, hardness and springiness decreased during 2 days of storage. The internal structure of the mulberry rice, observed by SEM, showed a close structure on increasing the coating ratios of mulberry leaves extracts. It was concluded that the optimum coating ratio of mulberry rice and ratio of added water for cooking whereas 1.5 and 140%, respectively, in proportion to the total weight of raw mulberry rice.

인공타액 오염이 수종 상아질접착제와 상아질간의 결합에 미치는 영향

오원만,양규호,류미애 大韓齒科保存學會 1992 Restorative Dentistry & Endodontics Vol.17 No.2

The purpose of this study was to evaluate the influence of artificial saliva contamination on bonding of several dintin adhesives to dentin. Sixty-three human molar teeth extracted within a month were used. Each tooth was sectioned longitudinally in a buccolingual direction to obtain 126 wpecimens. These specimens were randomly divided into three froups and were treated by Scotchbond 2, Gluam and All vond. Each group was subdivided into three subgroups; normal group not contaminated with artificial saliva, contaminated with artificial saliva and dried group, and contaminated with artificial saliva and washed and dried group. Enamel/dentin bonding agent (Dental Adhesive of Scotchbond 2) was applied light cured on the treated dentin surfaces. Threrafter P-50 were cured on them, and specimens were storde in 37℃ artificial saliva for 24 hours before measuring shear bond strength. Shear bond strengths were determined using an universal testing machine with cross head speed 1mm/min and SEM examinations were conducted to evaluate the resin-dintin interface and degree of penetrating resin string into the dentinal tubules. The following results were obtained. 1. Normal gropqs ont contaminated with artificial saliva showed greater shear bond streagth than any other group contaminated with artificial saliva)P<0.01). 2. The shear bond strengths showed no significant difference between washed groups with distilled water and ont washed groups after contamination with artificial saliva(P>0.05). 3. In normal groups, the shear bond strength of A group was significantly greater than in any other group(P<0.01). 4. In S and G groups, fractrues after shear bond strength tests occured adhesively on resintooth interface in all specimens. But in A groups, fractrue of the normal group occured cohesively in dentin and fractrue of the contaminated groups coourde adhesively and cohesively. 5. On SEM examination, the number of resin strings penotrated into dentinal tubules were the greatest in normal groups, followed by, in descending oredr, wasghed groups and not washed groups after contamination with artificial saliva.

여대생의 흡연에 대한 태도 및 요인 분석 : 김천대학 여대생 중심

송애랑 ; 박지원 김천대학교 2004 김천대학교 논문집 Vol.25 No.-

The purpose of this study was to explore the attitudes about smoking and factor analysis among college women. The subject were 347 female students selected by random sampling from Gimcheon College in Korea. The data were collected by survey methods a period of 7days. The data were analyzed by SPSS 10.0 version, T-test. F-test and factor analysis. Analysis of the data resulted in identification of 8 factors in general group and 5 factors in smoking group. Major factor were involved in curiosity, viewpoint of smoking, health belief, friend-relationship, image, body weight loss, fault, and viewpoint of opposite sex The characteristics of smoking behavior: 1. The meaning of smoking behavior in college women is justifiable orregretable. 2. Smoking behavior is related to the perception of harmfulness to health, influence of 2nd generation. 3. Smoking in college women is not caused by antagonism toward male smokers. 4. The first smoking time was middle school students It is suggested that the results of this study may contribute to the development of strategies for the purpose of decreasing smoking behav-ior among female college students.

종교언어(宗敎言語)로서의 공안(話頭)

윤원철,강은애 서울대학교 종교문제연구소 2001 종교와 문화 Vol.0 No.7

계획되지 않은 아동의 입원시 정보제공이 어머니 스트레스 대처에 미치는 효과

최명애,박승현,권원경,김선구,안혜영,김윤경 서울대학교 간호대학 간호과학연구소 1999 간호학 논문집 Vol.13 No.1

The purpose of this study was to explore the effect of informational intervention on coping of mother's stress in unplanned childhood hospitalization. Thirty four mothers of the hospitalized children, 24 months to 60 months old, were randomly assigned to experimental and control group. Child behavioral information and parental role information was given to experimental group by booklet and cassette tape. The state-trait anxiety was measured within 12 to 24 hours following hospitalization, the state anxiety was measured during from 24 hours to 48 hours, and the parent participation with care of children, and the parent support during invasive procedures was measured during 48 hours to 72 hours after hospitalization. The data was collected from May, 1997 to October, 1998 at B hospital in Seoul. Data was analyzed with X²-test, non paired t-test, ANCOVA test and Wilcoxon test using SAS program. Results were obtained as follows: 1. There were no difference in the state anxiety during the period of hospitalization and after discharging from hospital between experimental and control group. 2. Experimental group showed significantly higher scores on the parent participation with care of children than control group(P=0.03). 3. The parent support during invasive procedures of experimental group was significantly greater than that of control group(P=0.0167). The results suggest that information intervention regarding child behavioral information and parental role information may enhance both the parent participation with care of children and the parent support during invasive procedures.

성별의 차이에 따른 셀프 서비스 테크놀러지 수용에 관한 연구

조원애 서울여자대학교 2010 여성연구논총 Vol.25 No.-

Customer participation in the service process becomes more prevalent across the industries. The advancement of technology enables firms to accelerate customer participation. Self-service technologies (SST) are replacing employees to provide customers the service they want, wherever and whenever. The purpose of this study is to propose a model that illustrates how gender moderates the relationship between the antecedent variables (i.e. usefulness, ease of use, need for interaction, and risk) and the attitude of prospective users toward SST. The model proposes that women will be more strongly influenced by such variables as ease of use and risk, while men will be more compelled by variables such as usefulness and risk 기업은 테크놀로지의 급속한 발전에 따라서 다양한 고객 서비스 형태를 제공하고 있다. 특별히 기업과 소비자의 관심이 제품 중심에서 서비스 중심으로 변화되면서 서비스를 통하여 경쟁우위를 창출하려는 노력은 더욱 치열해지고 있다(Vargo & Lusch, 2004). 다양한 서비스의 하나로 부상하는 것이 고객의 직접 참여를 통한 서비스의 향상이며, 이러한 고객의 직접적인 참여는 향후 기업의 경쟁력을 향상시키는 방안이 될 수 있다(Bendapudi & Leone, 2003; Prahalad & Ramaswamy, 2000). 서비스 과정에 고객이 직접적으로 참여하는 것은 고객의 입장에서는 자신의 제품과 서비스의 과정에 직접 참여하여 통제력을 얻게되고, 궁극적으로 자신이 원하는 바를 이루는 것에 매력을 느끼는 경우가 많다. 또한 기업의 입장에서는 고객의 참여가 종업원의 노동력을 대체하는 수단이 될 수 있기 때문에 생산성 향상에 긍정적인 효과를 가져온다 (Fitzsimmons, 1985). 급격히 발전하고 있는 테크놀러지도 이러한 고객 참여의 주요한 원동력이 되고 있다. 특별히 테크놀로지를 통해서 고객을 직접 서비스 과정에 참여시키는 사례는 다양한 산업에서 전개되고 있다. 이렇게 서비스 과정에 테크놀러지를 도입하여 서비스 접점에서 고객과 종업원의 교류없이 스스로 서비스를 생산할 수 있도록 하는 것을 셀프서비스 테크놀러지(Self-Service Technology:이하 SST라고 함)라고 한다 (Meuter, et al., 2000). 1980년대 이후 금융고객들에게 흔하게 사용되고 있는 ATM을 효시로 등장한 셀프서비스테크놀로지는 항공사의 체크인 키오스크에서 부터 수퍼마켓의 자동 체크아웃 시스템으로 발전하였으며, 또한 인터넷을 기반으로 다양한 SST가 우리 일상에 정착되어 가고 있다. SST는 기업에게 인건비 절감 및 효율성 증대를 가져오고, 고객의 입장에서도 서비스를 받기 위하여 오랫동안 기다리지 않아도 되며, 능력없는 직원과의 대면을 피할 수 있는 장점이 있다 (Meuter et al 2000). 또한, 고객 자신의 거래를 직접 통제하기 때문에 안전하게 느끼는 점도 SST의 장점이라고 할 수 있다(Dabhokar, 1996). 하지만 기업 경쟁우위 창출의 수단으로 SST가 항상 성공을 기약하는 것은 아니다. 기업은 SST를 도입할 때 좀 더 적극적이고 올바른 방법으로 소비자가 사용할 수 있도록 하는 것이 중요하다. 따라서, 소비자의 SST에 대한 긍정적인 태도와 활발한 사용을 위하여 영향을 미치는 요인들이 무엇인지 연구 할 필요가 있다. SST의 성공적 도입에 영향을 주는 변수에 관한 기존의 연구들이 다소 있으나, 소비자의 성별, 나이, 교육 등 개인별 특성을 고려한 연구는 찾아 볼 수 없다. 실제로 새로운 IT를 도입할 때 성별, 나이, 문화 등의 변수에 따라 사람들의 새로운 기술의 수용태도나 활용수준이 다르다. 특별히, 다수의 IT관련 연구가 밝히고 있는 것 처럼 IT에 대한 남녀의 차이는 매우 크므로 이러한 성별의 차이가 SST 도입에도 예외는 아닐 것이다. 따라서, 본 연구의 목적은 SST의 성공적인 도입을 위하여 선행되어야 할 요인들을 밝혀내고, 성별의 차이가 어떤 역할을 하는 지를 제안하고자 한다. 연구는 Davis et al., (1989)의 기술 수용 모델을 근거로 성별의 차이에 따른 SST 수용 모델을 제안하고자 한다.

小續命湯 抽出物이 全腦虛血 및 局所腦虛血에 미치는 影響

李慶愛,辛吉祚,李源哲 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

이 실험의 목적은 小續命湯에 대하여 KCN(potassium cyanide)으로 유발된 혼수시간과 생존시간을 측정하여 全腦虛血에 미치는 영향을 관찰하고, 또한 MCA occlusion 유발시 腦浮腫率과 腦虛血面積을 측정하여 局所腦虛血에 미치는 영향을 실험연구하였다. 1. 전뇌허혈 유발실험에서 유의성있는 KCN 유발 혼수시간의 단축, 치사량의 KCN에 대한 생존시간의 연장 효과가 나타났다. 2. 국소 뇌허혈 유발실험(MCA occlusion)에 미치는 효과에서는 뇌허혈면적과 뇌부종율이 대조군에 비하여 유의성있게 감소하였다. The effects of Sosokmyung-tang(小續命湯) on global cerebral ischemia and cerebral in farction by MCA(middle cerebral artery) occlusion were evaluated in this study. This study was performed to investigate that Sosokmyung-tang would be useful for cerebrovascular diseases. In the case of global cerebral ischemia, ICR mice were used and divided into three group at random. Control group was treated after oral administration of normal saline, experimental group was treated after oral administration of 10,4㎎/20g/day of Sosokmyung-tang extract. The multiple parameter of global cerebral ischemia included the duration of coma of KCN(potassium cyanide)-injected(1.2㎎/㎏,i.v) group and the survival time of KCN-injected(3.0㎎/㎏,i.v) group. In the case of cerebral infarction by MCA occlusion, Sprague-Dawley rats were used and divided into three group at random. Control group was given nothing before MCA occlusion, experimental group was given 157.2㎎/250g/day of Sosokmyung-tang extract before MCA occlusion. We investigated edema and ischemic ratio in 8 slices of rats' brain after MCA occlusion. The results were obtained as follows : 1. Sosokmyung-tang significantly shortened the duration of coma of KCN-injected (1.2㎎/㎏,i.v) group and lengthened the survival time of KCN-injected(3.0㎎/㎏,i.v) group. 2. Sosokmyung-tang significantly decreased cerebral edema and ischemic ratio in rats after MCA occlusion. From the above results, it was concluded that Sosokmyung-tang can be effectively applied to cerebrovascular diseases.

오디 추출액 첨가비율에 따른 오디편의 품질특성에 관한 연구

김애정,김미원,우나리야,김명희,임영희 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.6

Oddi is a mulberry (Morus alba L) fruit which has antiinflammatory and antioxidative effects. This study was carried out to investigate the quality characteristics of Oddi-Pyun which was manufactured with various addition levels (0, 0.5, 1, 2, 4%) of Oddi extract according to the traditional Korean Kwaypun (a kind of jelly) methodology. Oddi-Pyun was made with various levels of Oddi extract, mungbean starch (9%) and sugar (30%). To establish the additional amount of mulberry fruit extract, sensory evaluation and physical test were carried out. From the results of sensory evaluation, the 2% of Oddi-Pyun was judged as the best in terms of color, flavor, hardness and elasticity. According to texture characteristics (hardness, adhesiveness, springiness, gumminess and chewiness), 1.0% and 2.0% of Oddi-Pyun were judged as the best. The contents of Ca (69.50, 74.75, 84.25, 100.60, 183.55ppm), Mg(27.37, 29.38, 34.20, 46.73, 97.45ppm) and Fe (320.23, 572.45, 680.50, 725.95, 906.50ppb) increased with increasing Oddi extract. Therefore, the optimal added amount of extract for the manufacture of Oddi-Pyun was proposed as 2% to the total weight. It was concluded that this Oddi-Pyun would be helpful to improve the health status of rheumatics and patients with similar diseases.