Cloning and Prokaryotic Expression of the Mature Fragment of the Chinese Yellow Bovine Myostatin Gene

Lu, Wenfa,Zhao, Jing,Wei, Guojian,Shan, Wuesong Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.6

Myostatin is a member of the transforming growth factor-${\beta}$(TGF-${\beta}$ super-family. It acts as a negative regulator for skeletal muscle growth. Myostatin mutations are characterized by a visible, generalized increase in muscle mass in double muscled cattle breeds. To understand the biochemistry and physiology of the Chinese Yellow bovine myostatin gene, we report here for the first time expression of the gene in Escherichia coli (E. coli). Primers of the myostatin gene of Chinese Yellow Cattle were designed on the basis of the reported bovine myostatin mRNA sequence (Gen-Bank Accession No. NM005259) and optimized for E. coli codon usage. XhoI and EcoRI restriction enzyme sites were incorporated in the primers, and then cloning vector and expression vector were constructed in a different host bacterium. The expressed protein had a molecule mass of about 16 kDa as determined by SDS-PAGE under reducing conditions. The expressed protein reacted specifically with myostatin monoclonal antibody on immunoblots. Our studies should lead to the investigation of the differences in myostatin genes of various cattle and could benefit human health and food animal agriculture.

Angle-stable top-emitting white organic light-emitting devices employing a down-conversion layer

Shihao Liu,Xuemei Wen,Wenbo Liu,Wei Zhang,Jing-Yuan Wang,Wenfa Xie,Letian Zhang 한국물리학회 2014 Current Applied Physics Vol.14 No.11

Angle-stable white top-emitting organic light-emitting devices (WTEOLEDs) by the combination of a blue TEOLED with a down-conversion (DC) layer are demonstrated. The DC layer is composed of red fluorescent dye doped in a host of tris (8-hydroxy-quinolinato) aluminum. The TEWOLED shows a CIE coordinates of (0.240, 0.332) and a maximum current efficiency of 3.95 cd/A. Furthermore, theWTEOLED shows excellently angle-stable characteristic, the CIE coordinates at 20 mA/cm2 only shift by (0.023, 0.007) from 0 to 60. It can be attributed to the angle-stable blue TEOLED based on the Ag/Ge/Ag transparent cathode.

SSR analysis reveals molecular variation and genetic diversity in Malus sieversii with different stress resistance levels in China

Shaoshuai Yu,Wenxia Zhao,Yanxia Yao,Wenxia Huai,Yuan Cheng,Zhongfu Zhou,Wenfa Xiao,Weiquan Qin,Wei Yan,Weiwei Song 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.6

Malus sieversii, a valuable crop in Xinjiang, China, is an important natural resource for researching the genetic diversity and phylogenetic evolution of the genus Malus worldwide. Samples from 152 M. sieversii individuals with different symptom grades of deadwood were collected from Gongliu and Xinyuan and analysed with 21 pairs of simple sequence repeat (SSR) primers, revealing molecular genetic characteristics and phylogeny of different groups and individuals. The samples showed high genetic diversity at the species level (Na = 10.76, Ne = 4.37, H = 0.73, I = 1.65, Ho = 0.71, and He = 0.73). Variation was mainly found within groups with lower genetic differentiation (Fst = 0.092) and higher gene flow (Nm = 2.67). A total of 226 alleles were obtained, of which 25 exclusive alleles were from samples with < 40% deadwood rate, and 23 exclusive alleles were from samples with > 40% deadwood rate. Specific bands relating to individuals with deadwood rate < 10% (10 exclusive alleles) or > 60% (7 exclusive alleles) were amplified with 11 pairs of SSR primers. The number of exclusive alleles from M. sieversii plants collected in Gongliu was 52 and from those collected in Xinyuan was 24. Using unweighted pair-group method with arithmetic cluster analysis, the groups with different symptom grades from different sampling sites were shown to be clearly differentiated and formed several discrete subclades. Significantly, the six groups from Gongliu were further classified into two subclusters: Gongliu 1, including three groups with < 40% deadwood rate, and Gongliu 2, including three groups with > 40% deadwood rate.

Effect of gold nanoparticles on the performances of the phosphorescent organic light-emitting devices

Kai Xu,Yang Li,Wei Zhang,Letian Zhang,Wenfa Xie 한국물리학회 2014 Current Applied Physics Vol.14 No.1

Gold nanoparticles (GNPs) on the performance of the phosphorescent organic light-emitting devices (OLEDs) were investigated. The green phosphorescent OLEDs with GNPs incorporated in hole transporting layer (HTL) or hole blocking layer (HBL) were fabricated using thermal evaporation technique. The results indicated that the performance of the OLEDs with GNPs were dependent on the position of the GNPs. The optimized device with GNPs in HBL shows enhanced current efficiency and reduced efficiency roll-off. However, the efficiency of the device with GNPs in HTL was decreased. The detailed physical mechanism is investigated in order to unveil such difference.

Multilevel memory characteristics by light-assisted programming in floating-gate organic thin-film transistor nonvolatile memory

Jun Ying,Jinhua Han,Lanyi Xiang,Wei Wang,Wenfa Xie 한국물리학회 2015 Current Applied Physics Vol.15 No.7

In this work, we study charge trapping in floating-gate organic thin-film transistor nonvolatile memories (FG-OTFT-NVMs) fabricated by a simple method. The inner discrete distribution aluminum nanoparticles (Al-Nps) and the continuous compact thin alumina film were formed to act as the floating-gate and the tunneling dielectric layer, respectively by thermally evaporated Al at a slow rate and then heat annealed in dry air. The devices exhibited remarkable photoresponse and memory effect. Compared with the unidirectional threshold voltage (VT) shifts of memories by programming/erasing (P/E) in dark, larger bidirectional VT shifts were obtained by light-assisted programming, and therefore the memory performances were enhanced. A multilevel memory behavior was observed in our memories, which depended on programming conditions. The charge trapping mechanisms of memories operated in dark and under illumination are discussed, respectively. The results indicate that optimal memory performance requires charge carriers of both polarities, because it is a very efficient method to enlarge the memory window and to lower the P/E voltage by overwriting trapped charges by injected charges of opposite polarity.

Effect of kisspeptin on the proliferation and apoptosis of bovine granulosa cells

Hongyu Liu,Myeong-Don Joo,Ji-Yoon Hwang,Ayman Mesalam,Jun Wang,Wenfa Lu,Il-Keun Kong 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

Previous studies have shown that kisspeptin (Kp-10) is expressed in mammalian ovaries; however, the expression and role of Kp-10 in bovine ovarian granulosa cells are still unclear. In this study, we assessed the expression of Kp-10 and its effects on the proliferation and apoptosis of bovine granulosa cells. Immunohistochemical analysis showed that Kp-10 was expressed in the cytoplasm of bovine ovarian granulosa cells. Moreover, MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2- H-tetrazolium bromide) assays showed that 100 nM Kp-10 significantly inhibited the viability of granulosa cells (P<0.01). Flow cytometry analysis showed that Kp-10 could significantly increase accumulation of cells in the G1 phase, decrease accumulation of cells in the S phase, and promote apoptosis in bovine granulosa cells (P<0.05). Additionally, Kp-10 decreased the mRNA levels of Bcl-2, an anti-apoptotic gene; increased the mRNA levels of caspase-3, a pro-apoptotic gene; and increased the mRNA levels of Fas and Fasl, two membrane surface molecule genes (P<0.05). Thus, our findings demonstrated for the first time that Kp-10 inhibited proliferation and promoted apoptosis in bovine ovarian granulosa cells. These findings provide insights into our understanding of the role of Kp-10 in mediating the proliferation of bovine granulosa cells.

Efficient fluorescent white organic light-emitting devices with a reduced efficiency roll-off based on a blue ambipolar fluorescent emitter

Tianyu Zhang,Tong Li,Wei Hu,Weiqi Wang,Letian Zhang,Wenfa Xie 한국물리학회 2014 Current Applied Physics Vol.14 No.5

White organic light-emitting devices (WOLEDs) with fluorescent donor-acceptor-substituted spirobifluorene compounds (red 2-diphenylamino-7-(2,2-dicyanovinyl)-9,90-spirobifluorene and blue 2- diphenylamino-7-(2,2-diphenylvinyl)-9,90-spirobifluorene) have been fabricated. The optimized WOLEDs shows a maximum current efficiency 5.9 cd/A and very low efficiency roll-off. From the brightness at maximum current efficiency to high brightness of 10000 cd/m2, the current efficiency rolloff is only 0.4%. It can be attributed to the ambipolar blue fluorescent emitter with voltage-independnet mobility which makes the device having a broader charge recombination zone and balance of carrier transport.

High efficiency top-emitting white organic light-emitting devices with a (metal/organic)_2 cathode

Wenyu Ji,Letian Zhang,Mo Liu,Jing Wang,Guoqiang Liu,Wenfa Xie,Hanzhuang Zhang 한국물리학회 2011 Current Applied Physics Vol.11 No.6

White top-emitting organic light-emitting devices (TEOLEDs) were fabricated on a glass substrate with metal/organic multilayer of (Ag/Alq_3)_2 (Alq3 is tris-(8-hydroxyquinoline) aluminum) as cathode. White TEOLEDs with high efficiencywere obtained due to the microcavity effects.Andthe (Ag/Alq_3)_2 cathode,which adjusted the optical characteristics of the devices, played an important role. In addition, Alq_3―Ag―Alq_3multilayer could work as a buffer layer, which would simplify the process of encapsulation for devices.We also calculated the electroluminescence spectrum of devices encapsulated with Al_2O_3 (150 nm) and Al_2O_3(75 nm)/ZrO_2(75 nm). And the results indicated that the CIE coordinates is almost the same between with and without encapsulating.