Protective Effect of Andrographolide on Alleviating Chronic Alcoholic Liver Disease in Mice by Inhibiting Nuclear Factor Kappa B and Tumor Necrosis Factor Alpha Activation

Yuan Song,Xiangqun Wu,Di Yang,Fang Fang,Lingshi Meng,Ya Liu,Weiwei Cui 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.4

Much research has indicated that alcoholic liver disease (ALD) is associated with oxidative stress and inflammation induced by ethanol, and that numerous antioxidants could effectively alleviate such injuries. Moreover, recent studies have identified andrographolide (AD) as having strong anti-inflammatory and antioxidant activities, which can block oxidative damage associated with nuclear factor kappa B (NF-κB)-mediated inflammation. However, the biological role and potential mechanism of AD in its protection against ALD have not been fully characterized. To observe the possible effect of AD, male C57BL/6J mice received ethanol through intragastrical gavage for 12 weeks in this study. The ethanol group was separated into five subgroups: (1) model group (n = 10); (2) silymarin group (0.1 mg/g body weight [BW], n = 10); (3) AD (0.05 mg/g BW) group (n = 10); (4) AD (0.1 mg/g BW) group (n = 10); and (5) AD (0.2 mg/g BW) group (n = 10). Mice in AD groups were treated orally by gavage once per day. The experimental results show that serum aminotransferase, liver lipids, lipid peroxidation, and antioxidant capacities were significantly changed in the model group after alcohol treatment, and the liver tissue histological findings showed pathological changes. Compared with the model group, treatment with AD improved serum aminotransferase, liver function, lipid accumulation, and hepatic reactive oxygen species levels. And AD decreased the hepatic NF-κB and tumor necrosis factor alpha (TNF-α) protein expression of ALD mice. This research demonstrated that AD can alleviate liver pathological injury and oxidative stress in mice exposed to ethanol by decreasing the expression of NF-κB and TNF-α.

Origin of Improved Photoelectrochemical Water Splitting in Mixed Perovskite Oxides

Li, Weiwei,Jiang, Kai,Li, Zhongguo,Gong, Shijing,Hoye, Robert L. Z.,Hu, Zhigao,Song, Yinglin,Tian, Chuanmu,Kim, Jongkyoung,Zhang, Kelvin H. L.,Cho, Seungho,MacManus-Driscoll, Judith L. Wiley (John WileySons) 2018 ADVANCED ENERGY MATERIALS Vol.8 No.31

Gallium(III) Nitrate Inhibits Pathogenic Vibrio splendidus Vs by Interfering with the Iron Uptake Pathway

( Tongxiang Song ),( Xuelin Zhao ),( Yina Shao ),( Ming Guo ),( Chenghua Li ),( Weiwei Zhang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.6

It is well known that iron is critical for bacterial growth and pathogenic virulence. Due to chemical similarity, Ga³⁺ competes with Fe3+ for binding to compounds that usually bind Fe³⁺, thereby interfering with various essential biological reactions. In our present study, gallium(III) nitrate [Ga(NO₃)₃] could repress the growth of V. splendidus Vs without complete inhibition. In the presence of Ga(NO₃)₃, the secretion of homogentisic acid-melanin (HGAmelanin) in V. splendidus Vs cells could be increased by 4.8-fold, compared to that in the absence of Ga(NO₃)₃. HGA-melanin possessed the ability to reduce Fe³⁺ to Fe²⁺. In addition, HGA-melanin increased the mRNA levels of feoA and feoB, genes coding Fe2+ transport system proteins to 1.86- and 6.1-fold, respectively, and promoted bacterial growth to 139.2%. Similarly, the mRNA expression of feoA and feoB was upregulated 4.11-fold and 2.71-fold in the presence of 640 μM Ga(NO₃)₃, respectively. In conclusion, our study suggested that although Ga(NO₃)₃ could interfere with the growth of V. splendidus Vs, it could also stimulate both the production of Fe³⁺-reducing HGA-melanin and the expression of feoA and feoB , which facilitate Fe²⁺ transport in V. splendidus Vs.

Combinatorial nanococktails via selfassembling lipid prodrugs for synergistically overcoming drug resistance and effective cancer therapy

Tongyu Li,Weiwei Shi,Jie Yao,Jingyun Hu,Qiong Sun,Jing Meng,Jian Wan,Haihan Song,Hangxiang Wang 한국생체재료학회 2022 생체재료학회지 Vol.26 No.1

Background: Circular RNAs (circRNAs) have important functions in many fields of cancer biology. In particular, we previously reported that the oncogenic circRNA, circPRMT5, has a major role in bladder cancer progression. Therapy based on circRNAs have good prospects as anticancer strategies. While anti-circRNAs are emerging as therapeutics, the specific in vivo delivery of anti-circRNAs into cancer cells has not been reported and remains challenging. Methods: Synthesized chrysotile nanotubes (SCNTs) with a relatively uniform length (~ 200 nm) have been designed to deliver an siRNA against the oncogenic circPRMT5 (si-circPRMT5) inhibit circPRMT5. In addition, the antitumor effects and safety evaluation of SCNTs/si-circPRMT5 was assessed with a series of in vitro and in vivo assays. Results: The results showed that SCNTs/si-circPRMT5 nanomaterials prolong si-circPRMT5’s half-life in circulation, enhance its specific uptake by tumor cells, and maximize the silencing efficiency of circPRMT5. In vitro, SCNTs encapsulating si-circPRMT5 could inhibit bladder cancer cell growth and progression. In vivo, SCNTs/si-circPRMT5 inhibited growth and metastasis in three bladder tumor models (a subcutaneous model, a tail vein injection lung metastatic model, and an in situ model) without obvious toxicities. Mechanistic study showed that SCNTs/sicircPRMT5 regulated the miR-30c/SNAIL1/E-cadherin axis, inhibiting bladder cancer growth and progression. Conclusion: The results highlight the potential therapeutic utility of SCNTs/si-circPRMT5 to deliver si-circPRMT5 to treat bladder cancer. Keywords: Synthesized chrysotile nanomaterials, Gene therapy, Targeted delivery, CircPRMT5, SiRNA, Bladder cancer

Density measurement and equal density temperature of CO2+brine from Dagang - formation from 313 to 363 K

Yi Zhang,Weiwei Jian,Yangchun Zhan,Yongchen Song,Mingjun Yang,Jiafei Zhao,Yu Liu,Weiguo Liu,Yong Shen 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.1

Densities of CO2+Dagang - formation brine solution were measured by a magnetic suspension balance(MSB) in the pressure range from (10 to 18) MPa, at the temperatures from (313.15 to 363.15) K and CO2 mass frac-tions at 0, 0.0101, 0.0198 and 0.0299. The experimental results revealed that the solution densities increased linearlywith the increasing pressure and CO2 concentration, while decreasing with the increasing temperatures in the experi-mental range. When the temperature increased from (313.15 to 363.15) K, the slopes of the densities versus (vs.) CO2mass fractions decreased from (0.193 to 0.106) g·cm−3. A correlation equation was developed based on thermody-namic theory and experimental data. The absolute average deviation between the correlation equation and the experi-mental data was 0.05%, and the maximum deviation was 0.37% for the density of CO2+water/brine solution in com-mon geological storage conditions. According to the density of CO2 - free brine and apparent molar volume of CO2 inbrine, the equal density temperature (Te) of CO2+Dagang brine solution was obtained at 464.67 K when pressure is10MPa, which means that the density of brine dissolved with CO2 will be less than that of CO2-free brine when thetemperature is higher than 464.67 K at 10MPa. In this work the formation temperature of the Dagang oilfield reser-voir is from 313.15 K to 363.15 K, which is lower than the equal density temperature. Therefore, the safety of CO2 stor-age in Dagang oilfield reservoir can be guaranteed.

Synthesis of CdS, ZnS, and CdS/ZnS Core/Shell Nanocrystals Using Dodecanethiol

Jinzhong Niu,Weiwei Xu,Huaibin Shen,Sen Li,Hongzhe Wang,Lin Song Li 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

We report a new route to synthesize high quality zinc blende CdS and ZnS nanocrystals in noncoordinating solvent 1-octadecene, using dodecanethiol (DDT) molecules as both the sulfur source and surface capping ligands. Different reaction temperatures and Cd(Zn)/DDT molar ratios were tested to optimize the synthesis conditions. Absorption photoluminescence (PL) spectroscopy, Fourier transform infrared (FTIR) spectroscopy,X-ray diffraction (XRD) pattern, and transmission electron microscopy (TEM) were used to characterize assynthesized nanocrystals. The narrow half width at the half-maximum on the long wavelength side of the firstexcitonic absorption peak and TEM images demonstrated nearly monodisperse size distributions of asprepared CdS, ZnS, and CdS/ZnS core/shell nanocrystals. Only trap emissions of the nanocrystals were detected when the amount of DDT was excessive, this came from the strong quenching effect of thiol groups on the nanocrystal surfaces. After overcoating with ZnS shells, band-gap emissions of CdS nanocrystals were partially recovered.

The applications of flower-shaped ZnO-UHMWPE fibers in photocatalysis and composites

Huixin Liu,Weiwei Li,Zhong Wu,Yilong Zhou,Kaiyuan Pei,Wenhu Song,Shiting Li,Jinfeng Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

Ultra-high molecular weight polyethylene (UHMWPE) fiber is considered as one of the high-performancefibers. However, the inert surface of UHMWPE fiber limits its application in many fields. In order to optimizethe application of UHMWPE fibers in functional and composite fields, this work introduced a specialsurface structure of the UHMWPE fibers. The designed surface was formed by the flower-shaped ZnO (FZnO)crystals, which endowed the UHMWPE fibers with photocatalytic property. Moreover, F-ZnO on theUHMWPE fiber surface enhanced the roughness of the fiber, which improves the interfacial bondingstrength of fiber-reinforced composites. The experimental results showed that the surface wettabilityof the F-ZnO-UHMWPE fibers was improved significantly. Moreover, F-ZnO-UHMWPE fibers were usedas immobilized photocatalyst, and the degradation rate of rodamine B reached 53.3 %. F-ZnOUHMWPEfiber also could be used in fiber-reinforced composites fields. Compared with the pure rigidpolyurethane (RPU) and UHMWPE fibers/RPU composites, the tensile strengths of F-ZnO-UHMWPEfibers/RPU composites were improved by 130.3 % and 44.2 % respectively.

Effect of Outdated Channel Estimates on Multiple Antennas Multiple Relaying Networks

( Lei Wang ),( Yueming Cai ),( Weiwei Yang ),( Wei Yan ),( Jialei Song ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.5

In this paper, we propose an intergraded unified imperfect CSI model and investigate the joined effects of feedback delay and channel estimation errors (CEE) for two-hop relaying systems with transmit beamforming and relay selection. We derived closed-form expressions for important performance measures including the exact analysis and lower bounds of outage probability as well as error performance. The ergodic capacity is also included with closed-form results. Furthermore, diversity and coding gains based on the asymptotic analysis at high SNRs are also presented, which are simple and concise and provide new analytical insights into the corresponding power allocation scheme. The analysis indicates that delay effect results in the coding gain loss and the diversity order loss, while CEE will merely cause the coding gain loss. Numerical results verify the theoretical analysis and illustrate the system is more sensitive to transmit beamforming delay compared with relay selection delay and also verify the superiority of optimum power allocation. We further investigate the outage loss due to the CEE and feedback delays, which indicates that the effect of the CEE is more influential at low-to-medium SNR, and then it will hand over the dominate role to the feedback delay.

Synthesis of CdS, ZnS, and CdS/ZnS Core/Shell Nanocrystals Using Dodecanethiol

Niu, Jinzhong,Xu, Weiwei,Shen, Huaibin,Li, Sen,Wang, Hongzhe,Li, Lin Song Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

We report a new route to synthesize high quality zinc blende CdS and ZnS nanocrystals in noncoordinating solvent 1-octadecene, using dodecanethiol (DDT) molecules as both the sulfur source and surface capping ligands. Different reaction temperatures and Cd(Zn)/DDT molar ratios were tested to optimize the synthesis conditions. Absorption photoluminescence (PL) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) pattern, and transmission electron microscopy (TEM) were used to characterize assynthesized nanocrystals. The narrow half width at the half-maximum on the long wavelength side of the firstexcitonic absorption peak and TEM images demonstrated nearly monodisperse size distributions of asprepared CdS, ZnS, and CdS/ZnS core/shell nanocrystals. Only trap emissions of the nanocrystals were detected when the amount of DDT was excessive, this came from the strong quenching effect of thiol groups on the nanocrystal surfaces. After overcoating with ZnS shells, band-gap emissions of CdS nanocrystals were partially recovered.

lncRNA Epigenetic Landscape Analysis Identifies <i>EPIC1</i> as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer

Wang, Zehua,Yang, Bo,Zhang, Min,Guo, Weiwei,Wu, Zhiyuan,Wang, Yue,Jia, Lin,Li, Song,Caesar-Johnson, Samantha J.,Demchok, John A.,Felau, Ina,Kasapi, Melpomeni,Ferguson, Martin L.,Hutter, Carolyn M.,Sof Cell Press 2018 Cancer Cell Vol. No.

<P><B>Summary</B></P> <P>We characterized the epigenetic landscape of genes encoding long noncoding RNAs (lncRNAs) across 6,475 tumors and 455 cancer cell lines. In stark contrast to the CpG island hypermethylation phenotype in cancer, we observed a recurrent hypomethylation of 1,006 lncRNA genes in cancer, including <I>EPIC1</I> (epigenetically-induced lncRNA1). Overexpression of <I>EPIC1</I> is associated with poor prognosis in luminal B breast cancer patients and enhances tumor growth <I>in vitro</I> and <I>in vivo.</I> Mechanistically, <I>EPIC1</I> promotes cell-cycle progression by interacting with MYC through <I>EPIC1</I>'s 129–283 nt region. <I>EPIC1</I> knockdown reduces the occupancy of MYC to its target genes (e.g., <I>CDKN1A</I>, <I>CCNA2</I>, <I>CDC20</I>, and <I>CDC45</I>). MYC depletion abolishes <I>EPIC1</I>'s regulation of MYC target and luminal breast cancer tumorigenesis <I>in vitro</I> and <I>in vivo</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LncRNAs show a hypomethylation phenotype, in contrast to a CIMP phenotype in cancer </LI> <LI> <I>EPIC1</I> promotes breast tumorigenesis through regulating cancer cell-cycle progression </LI> <LI> <I>EPIC1</I> directly interacts with MYC protein through <I>EPIC1</I>'s 129–283 nt region </LI> <LI> <I>EPIC1</I> regulates MYC targets by enhancing MYC occupancy on its target promoters </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>