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- 저자 : Tatyana (검색결과 124 건)
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Tatyana A. Klochkova,곽민석,한종원,Taizo Motomura,Chikako Nagasato,김광훈 한국조류학회I 2013 ALGAE Vol.28 No.2
A new cold-adapted Arctic strain of Haematococcus pluvialis from Blomstrandhalvøya Island (Svalbard) is described. This strain is predominantly always in non-motile palmelloid stage. Transmission electron microscopy showed the presence of very thick cell wall and abundant lipid vesicles in the palmelloids, including red and green cells. The external morphology of the non-motile palmelloid and motile bi-flagellated cells of our strain is similar to H. pluvialis; however it differs from H. pluvialis in physiology. Our strain is adapted to live and produce astaxanthin in the low temperature (4- 10°C), whilst the usual growth temperature for H. pluvialis is between 20-27°C. Phylogenetic analysis based on 18S rRNA gene data showed that our strain nested within the Haematococcus group, forming a sister relationship to H. lacustris and H. pluvialis, which are considered synonymous. Therefore, we identified our Arctic strain as H. pluvialis.
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Tatyana A. Klochkova,김광훈 한국조류학회I 2005 ALGAE Vol.20 No.4
The growth of subaerial microalgae on historic buildings or various cultural properties causes discoloration and physico-chemical deterioration of the surfaces. We collected a subaerial chlorophyte, Chlorella sp., from the stone Standing Buddha statue at Jungwon Miruksazi, which is a national treasure of Korea, and found dormant, thickwalled spores with regular pentagonal ornamentation along with the vegetative Chlorella cells. The morphology of Chlorella resting spores was compared to that of the other green algal resting cells. The ornamented spores and smooth-walled vegetative cells revived in 2 weeks in a liquid freshwater medium and started reproduction by autospores. To our knowledge, the ability of Chlorella to form ornamented dormant spores in drought condition was not previously recorded. The ornamentation of spores would supplement taxonomic characteristics of this genus.
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Tatyana A. Klochkova,Kang-sup Yoon,John A. West,김광훈 한국조류학회I 2005 ALGAE Vol.20 No.3
Some marine coenocytic green algae could form protoplasts from the extruded protoplasm in seawater. The dissociated cell components of the coenocytic protoplasm could be reunited into live cells and, hence, the formation of new species by mixing protoplasms from different coenocytic cells has been predicted. Our results showed that an incompatibility barrier was present during protoplast formation in coenocytic algae to exclude foreign inorganic particles or alien cell components. No inorganic particles or alien cell components were incorporated into protoplast formed spontaneously in seawater. Even when the inorganic particles or alien cell and/or cell component were incorporated into protoplast in some experimental condition, they were expelled from the protoplast or degenerated within several days. A species-specific cytotoxicity was observed during protoplast hybridization between the protoplasms of Bryopsis spp. and Microdictyon umbilicatum. The cell sap of M. umbilicatum could destroy the cell components of Bryopsis spp., but had no effect on Chaetomorpha moniligera. Species C. moniligera and Bryopsis did not affect protoplast generation of either species. The wound-induced protoplast formation in vitro might have evolved in some coenocytic algae as a dispersal method, and the incompatibility barrier to alien particles or cell and/or cell component could serve as a protective mechanism for successful propagation.
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Tatyana A. Belan,Alexander A. Moschenko 한국해양과학기술원 2005 Ocean science journal Vol.40 No.1
in species diversity and abundance of polychaete taxocenes that occurred in 1980-1989 under different contamination levels of bottom sediments were studied in three areas of Peter the Great Bay. The most polluted area was shown to be the Golden Horn Inlet where contaminant contents in the bottom sediments exceed the threshold values of negative biota alterations. Amursky Bay is characterized by a moderate level of contamination, while Ussuriysky Bay has the lowest level of contamination. Pollutant contents vary considerably within the same areas and their separate patches are polluted differently. An integral index characterizing the contamination of bottom sediments is proposed. This index is an average grade of the rank value of contaminant contents in sediments. The index was used to compare the contamination level and data on polychaete species diversity and abundance. The highest species diversity of polychaetes is found in the least affected zones. Monotonous decrease of the species number, as well as decrease in the indices of diversity and evenness, is correlated with pollution level increases. Significant growth of the average polychaete biomass and polychaete density is observed in the case of an increase of contamination from low to moderate levels. Conversely, the biomass and abundance of polychaetes decline following an increase in contamination.
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( Tatyana Kunts ),( Ksenia Karpukhina ),( Dmitry Morozov ),( Maria Pustovetova ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Several immunohistochemical markers are known to predict the biological behavior of breast cancer and assess tumor aggressiveness. In particular, ER and PR receptors concern the occurrence of autocrine and paracrine processes that respond to estrogen and progesterone. Ki67 is a nuclear protein, marker proliferative activity of the tumor, high Ki67 usually correlates with a poor prognosis in breast cancer. There has been interest to observe the expression of immunohistochemical markers in comparison to histopathological features of infi ltrative ductal breast carcinoma. Methods: The study was carried out on the samples from 34 patients with infi ltrative ductal carcinoma. Scheme was elaborated to describe histopathological picture including following points: presence of tumor cells within blood vessels, cell infi ltration (lymphocytes, macrophages and granulocytes), the number of mitoses, the ratio of cell elements at various differentiation stage, vascularization, histological type of tumor, the number of affected lymph nodes. Steroid receptors and Ki67 expression were analyzed by immunohistochemical means. Statistical analysis was performed using Spearman`s nonparametric rank correlation coeffi cient test. Results: Proliferation marker Ki67 was directly connected with the presence of tumor cells within blood vessels (r=0.343, p=0.05), granulocytic cell infi ltration of the tumor (r=0,363, p=0.03), aberrant mitoses (r=0,423, p=0.01), relative immature cells content in the tumor (r=0.543, p=0.001). An inverse correlation between Ki67 and relative content of highly differentiated cells in the tumor (r= -0.388, p=0.02) was revealed. ER and PR indexes demonstrated direct correlation with the number of lymph nodes affected by metastases (r=0.468, p=0.005 and r=0.521, p=0.002, respectively). Conclusions: findings suggest expression of estrogen receptor, progesterone receptor and Ki67 are related to histopathological pattern of infi ltrative ductal breast carcinoma. Consequently, results of immunohistochemical analysis conducted on samples obtained by core biopsy should be used to evaluate tumor severity before the surgery.
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Tatyana A. Klochkova,Nina G. Klochkova,Norishige Yotsukura,김광훈 한국조류학회I 2017 ALGAE Vol.32 No.1
Morphological, molecular and chromosomal studies were carried out on Tauya basicrassa, an endemic kelp species distributed on the northern continental coast of the Sea of Okhotsk in Russia. The sporophytes of T. basicrassa grow up to 3–6 m long, 1.8–2.2 m wide, and 6.5–7 kg wet weight. The thallus has a blade with very thick narrow basal portion and thinner and much broader upper portion, which usually splits into 3 bullated lobes. A dwarf laminariacean alga, which did not show any morphological similarity to the other species of the order Laminariales, was found from the same locality. The blade of this alga is thin and soft, reached 26–34 cm long and 6–6.5 cm wide and had 4 longitudinal rows of bullations that covered the entire blade. Molecular analysis showed that the dwarf alga has 100% sequence identity in plastid-encoded RuBisCo spacer, mitochondrial cytochrome c oxidase subunit 1 and nuclear-encoded rDNA genes with normal sporophytes of T. basicrassa, indicating that they are different life forms of the same species. Fluorescent DAPI staining showed that the nucleus in the normal sporophyte was 50–65% larger than those of the dwarf ones. Chromosome count using acetocarmine staining showed n = ca. 20 for the normal sporophytes of T. basicrassa and n = ca. 10 for the dwarf one. These results suggest that the dwarf thallus is a haploid parthenosporophyte of T. basicrassa, which developed in nature. This is the first evidence of parthenosporophytes of the laminariacean algae occurring naturally in the field.
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Tatyana A. Klochkova,김광훈,Mihail N. Belij,Nina G. Klochkova 한국조류학회I 2012 ALGAE Vol.27 No.3
The re-examination of morphological and anatomical characters of Laminaria appressirhiza and L. inclinatorhiza collected from different localities in the Sea of Okhotsk was performed. Despite their commercial and ecological importance to the region they have not been comprehensively reviewed since their first description in 1970. Our results show that some original diagnostic key characters (e.g., shape of holdfast, shape of sporangial sori, and dissection of blade) are not stable and have deviations from the type morphology when plants grow in different environments. In L. inclinatorhiza, the sporangial sori development occurred differently to the pattern indicated in original species description as they did not develop simultaneously on both sides of the blade. Instead, the sporangial sori outlines on both sides of the blade did not coincide at first and only became coincident later. Also, a deep-water population of L. inclinatorhiza with an unusual and interesting morphology, growing at depths of 15-25 m on opened rocky coasts in Taujskaya Bay (northern part of the Sea of Okhotsk) was found. The stable diagnostic key characters to distinguish these two species are the cone-like, multilayered, very thick and massive holdfast (in L. inclinatorhiza) and rolled margins of blades, lamellar rosette-like part of thallus, and sporangial sori developing only on one side of the blade (in L. appressirhiza). The ecological characteristics, distribution, and abundance of both species in the Sea of Okhotsk are discussed. Both species are perennial and widely distributed in the region. L. appressirhiza is more often found as a subdominant species among other kelps, forming maximum biomass and density of 7-9 kg and 8-25 plants per 1 m2, respectively. L. inclinatorhiza sometimes forms local mono-species communities with maximum biomass and density of 10-12 kg and 10-15 plants per 1 m2, respectively.
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