생체흡착, 탈착 및 회화를 이용한 시안 용액으로부터 금의 회수

배민아(Min A Bae),곽인섭(In-Seob Kwak),원성욱(Sung Wook Won),윤영상(Yeoung-Sang Yun) 한국청정기술학회 2010 청정기술 Vol.16 No.2

본 연구에서는 Au(I) 이온을 함유한 시안 용액으로부터 다른 형태의 금을 회수하기 위하여 생체흡착 후 탈착하는 방법과 생체흡착 후 회화하는 방법을 제안하였다. 생체흡착제로는 아미노산 발효 공정에서 발생하는 균체 폐기물(Corynebacterium glutamicum)을 사용하였다. 바이오매스의 흡착 특성을 알아 보기 위하여 pH edge 실험을 수행하였으며, 그 결과 흡착성능은 pH 2-3 부근에서 우수한 결과를 보였다. 또한, 등온흡착 실험 결과를 Langmuir 모델에 적용한 결과 Au(I)의 최대 흡착량은 pH 2.5에서 35.15 mg/g이었다. 흡착 속도론 실험을 통해 흡착 평형은 60분 이내의 짧은 시간 내에 이루어지는 것을 확인하였다. Au(I)을 회수하기 위하여 바이오매스에 흡착된 Au(I)을 탈이온수를 이용하여 탈착을 시켰으며, 탈착효율은 91%로 평가되었다. 이 결과는 바이오매스를 이용하여 흡착과 탈착을 통해 1가 금을 효과적으로 회수할 수 있음을 보여준다. 영가 형태로 회수하기 위하여, 금을 흡착하고 있는 생체흡착제를 회화하여 환원된 형태의 금을 성공적으로 회수하였으며, 회분 중 금의 순도는 85% 이상이었다. 따라서 본 연구에서 제시한 생체흡착 후 탈착(1가 형태) 공정과 생체흡착 후 회화(영가 형태) 공정은 회수하고자 하는 금의 산화수에 따라 선택하여 사용될 수 있을 것으로 기대된다. In this study, we propose two methods able to recover different type of gold from gold-cyanide solutions: biosorption and desorption process for mono-valent gold recovery and biosorption and incineration process for zero-valent gold recovery. The waste bacterial biomass of Corynebacterium glutamicum generated from amino acid fermentation industry was used as a biosorbent. The pH edge experiments indicated that the optimal pH range was pH 2 - 3. From isothermal experiment and its fitting with Langmuir equation, the maximum uptake capacity of Au(I) at pH 2.5 were determined to be 35.15 mg/g. Kinetic tests evidenced that the process is very fast so that biosorption equilibrium was completed within the 60 min. To recover Au(I), the gold ions were able to be successfully eluted from the Au-loaded biosorbent by changing the pH to pH 7 and the desorption efficiency was 91%. This indicates that the combined process of biosorption and desorption would be effective for the recovery of Au(I). In order to recover zero-valent gold, the Au-loaded biosorbents were incinerated. The content of zero-valent gold in the incineration ash was as high as 85%. Therefore, we claim on the basis of the results that two suggested combined processes could be useful to recover gold from cyanide solutions and chosen according to the type of gold to be recovered.

Effects of Dilute Acid Pretreatment on Enzyme Adsorption and surface Morphology of Liriodendron tulipifera

Byeong Cheol Min,Bon Wook Koo,Ki Seob Gwak,Hwan Myeong Yeo,Joon Weon Choi,In Gyu Choi 한국목재공학회 2011 목재공학 Vol.39 No.2

In this study, dilute acid pretreatment of Liriodendron tulipifera was performed for enzymatic hydrolysis. As the pretreatment temperature was increased, enzymatic hydrolysis and enzyme adsorption yield also increased. The highest enzymatic hydrolysis yield was 57% (gig) and enzyme adsorption was 44% (gig). Enzymatic hydrolysis yield was determined with weight loss of pretreated biomass by enzyme, and enzyme adsorption was a percentage of enzyme weight attaching on pretreated biomass compared with input enzyme weight. When L. tulipifera was pretreated with 1% sulfuric acid at 160℃ for 5 min., hemi-cellulose was significantly removed in pretreatment, but the lignin contents were constant. Other changes in surface morphology were detected on biomass pretreated at 160℃ by a field emission scanning electron microscope (FESEM). A large number of spherical shapes known as lignin droplets were observed over the entire biomass surface after pretreatment. Hemicellulose removal and morphological changes improved enzyme accessibility to cellulose by increasing cellulose exposure to enzyme. It is thus evidence that enzyme adsorption is a significant factor to understand pretreatment effectiveness.

Lactobacillus pentosus 발효에 의한 담쟁이덩굴 줄기 추출물의 항산화 및 세포보호 효과

박소현 ( So Hyun Park ),성준섭 ( Joon Seob Seong ),이건수 ( Keon Soo Lee ),박영민 ( Young Min Park ),현송화 ( Song Hua Xuan ),차미연 ( Mi Yeon Cha ),강희철 ( Hee Cheol Kang ),박수남 ( Soo Nam Park ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.3

본 연구에서는 담쟁이덩굴 줄기 70% 에탄올 추출물과 발효균주 Lactobacillus pentosus를 이용하여 발효시킨 담쟁이덩굴 줄기 발효추출물에 대하여 항산화 및 세포보호 효과를 측정하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH)를 이용한 자유라디칼 소거 활성(FSC₅₀)은 담쟁이덩굴 줄기 추출물 및 발효추출물이 각각 42.3 및 34.5 μ g/mL로 발효 후의 라디칼 소거활성이 약 18.4% 더 높게 나타났다. Lumiol-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂계에서의 총 항산화능(OSC₅₀) 평가에서도 담쟁이덩굴 줄기 추출물과 발효추출물은 각각 2.6 및 2.5 μ g/mL로 발효 후가 약 4.2% 정도 더 높은 총 항산화능을 나타냈다. ¹O₂로 유도된 적혈구 세포손상에 있어서 추출물 및 발효추출물의 세포 보호 효과(τ₅₀)는 50 μ g/mL에서 각각 126.4 및 173.0 min을 나타내어 발효 후 세포 보호 효과가 약 34.0% 더 높게 나타났다. 발효추출물은 지용성 항산화제로 알려진 (+)-α-tocopherol (43.4 min)보다도 3.9배 높은 세포 보호 활성을 보여주었다. 사람 섬유아세포인 Hs68을 대상으로 elastase 저해 활성을 조사하였다. Elastase 저해 활성(IC₅₀)은 담쟁이덩굴 줄기 추출물과 발효추출물에서 각각 873.6 및 687.8 μ g/mL로 발효 후에 elastase 저해 활성이 약 21.3% 더 높은 것으로 나타났다. 이상의 결과들은 담쟁이덩굴 줄기 발효추출물이 항산화 작용과 더불어 주름개선 효과를 가지는 천연 화장품 소재로써 응용 가능성이 있음을 시사한다. In this study, the antioxidant activities, cellular protective effects, and inhibitory effects on elastase of non-fermented and fermented extracts of Parthenocissus tricuspidata (P. tricuspidata) stem using Lactobacillus pentosus were investigated. The free radical scavenging activities (FSC₅₀) of non-fermented and fermented extracts were 42.3 and 34.5 μg/mL, respectively, in which the activity after fermentation was approximately 18.4% higher. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) in Fe³⁺-EDTA/H₂O₂ system of non-fermented and fermented extracts were 2.6 and 2.5 μg/mL, respectively. The activity after fermentation was approximately 4.2% higher. In the ¹O₂-induced cellular damage of erythrocytes, the cellular protective effects (τ₅₀) of non-fermented and fermented extracts were 126.4 and 173.0 min at 50 μg/mL, respectively. The activity after fermentation was approximately 34.0% higher. The effect of fermented extract was 3.9 times higher than (+)-α-tocopherol (τ₅₀ = 43.4 min), known as a lipophilic antioxidant at 50 μg/mL. The inhibitory effect of elastase was investigated to predict the anti-wrinkle efficacy using Hs68 human fibroblasts cells. The elastase inhibitory activities (IC₅₀) of non-fermented and fermented extracts were 873.6 and 687.8 μg/mL, respectively, and the activity after fermentation was approximately 21.3% higher. These results indicated that fermented extract of P. tricuspidata stem has potentials as natural cosmetic ingredients with antioxidant and anti-wrinkle effect.

Effects of Chungkookjang Extract on Growth Hormone Secretion from GH3 Mouse Pituitary Cell and Growth Hormone Receptor Signaling Pathway

Sun Il Choi(최선일),Ji Eun Kim(김지은),In Sik Hwang(황인식),Hye Ryun Lee(이혜련),Young Ju Lee(이영주),Hong Joo Son(손홍주),Dong Seob Kim(김동섭),Kyu Min Park(박규민),Dae Youn Hwang(황대연) 한국생명과학회 2012 생명과학회지 Vol.22 No.9

뇌하수체 전엽에서 성장호르몬의 생산과 분비는 세포의 분열과 분화 그리고 이동을 조절하는 몇 가지 천연물질에 의해 유도된다. 따라서 발효과정을 통해 제조된 청국장이 성장호르몬의 대사에 미치는 영향을 평가하기 위하여 성장호르몬 분비능과 반응성을 뇌하수체 세포와 성장호르몬 표적세포에서 관찰하였다. 6가지 종류의 콩 품종으로 제조된 청국장 추출물 중에서, 대원, 대풍, 태광의 3종류 청국장 추출물은 5 mg/ml 농도에서 GH3 세포로부터 성장호르몬의 분비를 촉진하였다. 비록 세포 생존능은 이러한 추출물에 의해 유의적인 변화가 유도되지 않았으나, 성장호르몬의 분비량은 청국장 추출물의 농도에 의존적으로 증가하였다. 또한 성장호르몬의 표적 기관으로부터 유래된 MG63과 HepG2 세포는 GH3로부터 수집된 조건적 배양액에 의해 유의적으로 활성화되었다. 또한 이러한 세포에서 STAT5 발현은 대원 청국장 추출물을 처리 후, 15분 혹은 30분부터 세포질에서 유의적으로 증가하였으며, p-STAT5는 핵에서 30분 혹은 60분부터 증가하였다. 따라서 이러한 결과는 3가지 종류의 청국장 추출물은 성장호르몬의 분비를 촉진시키며, 청국장의 조건적 배양액은 성장호르몬 표적세포에서 신호전달을 유도함을 제시하고 있다. The production and secretion of growth hormone (GH) in the anterior pituitary gland can be induced by several natural products to control cell proliferation, differentiation, and migration. To investigate whether Chungkookjang (CKJ) produced by the fermentation process affects GH-related metabolism, the secretion and the response of GH were observed in pituitary cells and GH target cells. Among six CKJs manufactured by different strains of glycine max, only three CKJs, including Daewon (DW), Daepung (DP), and Taegwang (TG), induced GH secretion from GH3 cells at 5.0 mg/ml concentration. There were no significant changes detected in the viability of any of the cells treated with these CKJs. In addition, the increase in GH secretion from the GH3 cells was dependent on the concentration of the three types of CKJs. The proliferation of cell lines, including MG63 and HepG2 cells, that originated from those derived from the GH target organs was significantly activated by treatment with the GH-containing conditional medium (GCM) harvested from the three CKJ-treated GH3 cells, although their induction rate was different from each other. In these cells, p-STAT5 was maximally translocated into the nucleus of MG63 cells 30 min after DW treatment, while it was translocated in HepG2 cells at 60 min. These results suggest that these three types of CKJ could enhance the secretion of GH, as well as the GCM-derived response, in the two target organs.

국내 발전소별 바텀애시를 잔골재 대체재로 사용한 모르터의 특성에 대한 실험적 연구

송민섭,장재봉,조봉석,김재환,김용로,김무한 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

With the advance in industrial development and modern living comfort, the demand for electricity throughout the country is ever increasing. Also the annual consumption of coal by coal-fired power plants is increasing. The amount of ash produced in 2000 is estimated to be about 4.5 million tons. The disposal of this huge amount of ash introduces serious environmental problems. Fly ash among the ash produced is used in building industries as a substitute to cement in concrete and the concrete thus produced is said to be lighter and stronger. But bottom ash among the ash produced is not used because of its poor properties. But encouraging the use of bottom ash as a construction material is a sensible method of utilization as it avoids the problems and costs associated with disposal and provides an alternative aggregate source. This study is aimed at using bottom ash as an alternative fine aggregate source to provide a solution to disposal and insufficient fine aggregate for the production of concrete. So properties of bottom ash produced in power plants were estimated due to the difference of bottom ash produced in each plant. And mortar strength was estimated as basic data to use bottom ash in building industries.

정상 분만 후 발생한 대동맥 박리증

심민섭,송형곤,정연권,송근정 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Hypertension is a risk factor for developing fatal aortic dissection. Aortic dissection occurs 2~3 times as often in men than in women between ages of 50~70 years old. Among other risk factors, aortic dissection occurs especially in women below the age of 40 years old in pregnancy-related cases. In pregnancy-related cases, aortic dissection occurs n the third trimester and in puerperium, as well as in multiparous women. The writers experienced an aortic dissection related pregnancy. The patient was 32 years old, diagnosis of dissection occurred in the postpartum period. Although, aortic dissection is a rare complication of pregnancy, the knowledge of this may assist the emergency physician save the lives of patients.

급성기 가와사끼병 환아의 혈청 Vascular Endothelial Growth Factor 및 Endostatin에 대한 연구

송민섭,이승익 인제대학교 2008 仁濟醫學 Vol.29 No.-

Objective : Vascular Endothelial Growth Factor(VEGF) is an angiogenic growth factor that has been implicated in both physiological and pathological angiogenesis. Endostatin(ES) is a potent inhibitor of angiogenesis and exhibits antiangiogenic activity by inhibiting the proliferation and migration of endothelial cells(ECs) in addition to inducing endothelial cell apoptosis. Imbalance between angiogenic and antiangiogenic factor exists in subacute phase of KD, as pathologic angiogenesis occurs in subacute phase of Kawasaki disease(KD). The purpose of this study is to investigate whether an imbalance exists in the production between angiogenic and antiangiogenic growth factors in patients with acute phase of Kawasaki disease(KD), we measured the serum levels of VEGF and ES. Methods and Materials : Serum VEGF, ES levels and VEGF/ES ratio were measured in 30 KD patients, 10 incomplete KD patients and 12 febrile illness controls. Blood samples were drawn from each study group during acute phase before intravenous immunoglobulin(IVIG) therapy. Results : KD patients had higher VEGF levels(426.70 ± 479.63 pg/mL vs 297.71 ± 251.32 pg/mL, P=0.52) and VEGF/ES ratio(3.67 ± 5.69 vs 2.16 ±1.763, P=0.55 ) in the acute phase of KD than controls, but the difference was not significant. ES levels were within normal range and there was no difference of ES levels(132.65 ± 33.98 ng/mL vs 135.54 ± 25.95 ng/mL, P=0.97) between Kawasaki disease and controls in the acute phase. Conclusion : The findings in this present study indicate that an imbalance does not exist in the production between VEGF and ES in patients with acute phase of KD.

발기부전 검사에서 천연색 복합초음파촬영술과 야간음경발기검사의 일치도

송윤섭,구자현,김민의,이혜경,김두상,박영호,이남규 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

We intended to know that the result of color duplex ultrasonography corresponded to that nocturnal penile tumescence rigidity in the diagnosis of erctile dysfunction. We performed both color duplex ultrasonography and nocturnal penile tumescence rigidity in 15 erectile dysfunction men. Colr duplex ultrasonography was done with a 7 MHz. color Doppler unit after intracorporeal pharmacological injection and measured maximal arterail diameter, peak systolic velocity, end-diastolic velocity and the results were compared with rigidity and tumescence in nocturnal penile tumescence rigidity using RigiScan. The positive predictive value, negative predictive value, sensitivity, specificity, overall accuracy of color duplex ultrasonography was reasonable when the result of color duplex ultrasonography was compared with the that of nocturnal penile tumescence rigidity. According to this results, color duplex ultrasonography could not only predict but also correspond to nocturnal penile tumescence. Therefore, we suggest that color duplex ultrasonography can relpace nocturnal penile tumescence rigidity if psychogenic and neurogenic erectile dysfunction are distinguished by history and neurologic examination.

β-mercaptoethanol, hyaluronic acid, EGF가 돼지난포란의 체외수정과 체외발생에 미치는 영향에 관한 연구

김용섭,김민수,김상근 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

The study was carried out to evaluate the effects of β-mercaptoethanol(β-ME), hyaluronic acid(HA) and epidemal growth factor(EGF) supplemented to the TCM-199 culture media on in vitro fertilization and in vitro developmental rates of porcine oocytes. The porcine follicular oocytes and embryos were cultured in TCM-199 media containing hormones, β-ME(10, 50, 100 μM), HA (0.5, 1.0, 1.5 ㎎/㎖), EGF(5,8,10%) and PSA(10%) for 40-48 hrs and 8-9 days in a incubator with 5% CO_2 in air at 38.5℃. In vitro developmental rates were defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro feertilization and in vitro developmental rates of porcine oocytes cultured in TCM-199 media supplemented with 10, 50, 100 μM β-ME were 85/113(75.22%), 89/111(80.18%), 92/108(85.19%) and 28/113(24.78%), 36/111(32.43%), 30/108(27.78%), respectively. 2. The in vitro feertilization and in vitro developmental rates of porcine oocytes cultured in TCM-199 media supplemented with 0.5, 1.0, 1.5 ㎎/㎖ HA were 80/103(77.67%), 84/100(84.00%), 82/105(78.09%) and 27/103(26.21%), 35/100(35.00%), 30/105(28.57%), respectively. 3. The in vitro feertilization and in vitro developmental rates of porcine oocytes cultured in TCM-199 media supplemented with 5, 8, 10% EGF were 79/101(78.22%), 86/105(81.90%), 85/103 (82.52%) and 23/101(22.77%), 30/105(28.57%), 32/103(31.07%), respectively. 4. The in vitro feertilization and in vitro developmental rates of porcine oocytes cultured in TCM-199 media supplemented with 10% PSA and 50 u β-ME, 1.0 ㎎/㎖ HA, 8% EGF were 73/85(85.88), 70/80(87.50%), 71/82(86.59%) and 29/85(34.12%), 30/80(37.50%), 25/82(30.49%), respectively. The in vitro fertilization and developmental rates were significantly higher than those of non-supplemented PSA and β-ME, HA, EGF.

만성전립선염 환자에 있어서 항정자항체의 존재와 정자의 운동성 감소

송윤섭,김민의,박영호 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Chronic prostatitis may be related to the decrease of semen quality affecting fertility due to the existence of antisperm antibody. So we evaluated the characteristics of antisperm antibody in the control group and patients with chronic prostatitis. Statistical significance was noted in average path velocity(VAP), straight line velocity(VSL), curvilinear velocity(VCL), beat frequency(BCF), elongation of sperm motility parameters between the control group and patients with chronic prostatitis. Detection of antisperm antobody with direct and indirect immunobead test were 0%(0/11), 9.1%(1/11) in the control group and 6.3%(1/16), 37.5%(6/16) in the patients with chronic prostatitis. Titers of antisperm antibodies according to isotype (% binding, control group/ patients with chronic prostatitis) were 0/0.63±2.5(IgG), 0/0(IgA), 0.13±0.5/0(IgM) with direct immunobead test and 0.18±0.60/1.19±1.80(IgG), 0/0.06±0.25(IgG), 0/1.25±2.32(IgM) with indirect immunobead test. Statistical significance was noted in VAP, VCL, BCF between chronic prostatitis patients with and without antisperm antibody. The correlation between IgG and VAP, IgG and VCL existed. In conclusion, decreased sperm motility and the antispermantibody were found in chronic prostatitis. The antisperm antibody showed sharp contrast between the control group and the patients by indirect immunobead test, rather than by direct immunobead test. The existence of antisperm antibody correlated with sperm motility in chronic prostatitis.