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이주용,조준,박호권,장상근,문창택,한혜승,이경룡 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-
The authors in this manuscript aimed to provide a practical importance of the differential diagnosis of intracranial brain tumor with emphasis on non-neoplastic abnormalities such as cytotoxic edema of the penumbra zone in strokes. The well known SOLs are cerebral ischemic, inflammatory and dysplastic lesions, which intraaxially imitate glioma and other neurogenic tumors. Brain tumor is the one of common intracranial space-occupying lesions (SOLs) on the diagnostic images. However, many non-neoplastic lesions may be indistinguishable from tumors. Especially, early mortality used to be caused by space-occupying hyperacute ischemic stroke, brain edema and herniations. For the retrospective analysis, the authors reviewed the patient charts, the emergency brain Computerized Tomography (CT), magnetic resonance imaging (MRI), clinical stroke history. From Jan. 2003 to Dec. 2003, we managed total 61 acute strokes in ICU. Even though we operated six decompressive craniotomies, nine stereotactic craniotomies including two procedures of brain biopsy and three extraventricular drainage procedures, three patients (4.91%) were finally expired out from the induced herniation and medullary failure by those aggressive strokes. 43 patients (70.5%) were recovered good from stroke attack. 15 patients (24.6%) were improved with residual neurologic deficits. In conclusion, aside from growing efforts of the radiologist to make the early diagnostic impression, the authors suggest that urgent neurosurgical explorations with the pathologic diagnostic decision should be requested to decrease the fatality.
단계중합법에 의한 Core-Shell 구조의 Acrylic Composite Particle 제조에 관한 연구
이선룡,윤주섭,강돈오,설수덕 동아대학교 생산기술연구소 2001 生産技術硏究所硏究論文集 Vol.6 No.2
Core-shell composite latex has the both properties of core and shell components. This unique behavior of core-shell composite latex can be used in many industrial fields. However, in preparation of core-shell composite latex, several unexpected are observed, such as, particle coagulation, low degree of polymerization, and formation of new particles during shell polymerization. To solve the disadvantages, we study the effect of initiator concentrations, surfactant concentrations, and reaction temperature on the core-shell structure of Polymethyl methacrylate/polystyrene and polystyren/polymethyl methacrylate. Particle size and particle size distribution were measured by using Particle Size Analyzer, and the morphology of the core-shell composite latex was determined by using Transmission Electron Microscope. Glass temperature was also measured by using Differential Scanning Calorimeter. To identify the core-shell structure, pH of the two composite latex solutions were measured.
Jeong Byeong Ryong,Chung Su-Mi,Baek Nam Joo,Koo Kwang Bon,Baik Hyung Suk,Joo Han-Seung,Chang Chung-Soon,Choi Jang Won The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.1
Ferritin is a major eukaryotic protein and in humans is the protein of iron storage. A partial gene fragment of ferritin (255 bp) taken from the total RNA of Periserrula leucophryna, was amplified by RT-PCR using oligonucleotide primers designed from the conserved metal binding domain of eukaryotic ferritin and confirmed by DNA sequencing. Using the $^{32}P-labeled$ partial ferritin cDNA fragment, 28 different clones were obtained by the screening of the P. leucophryna cDNA library prepared in the Uni-ZAP XR vector, sequenced and characterized. The longest clone was named the PLF (Periserrula leucophryna ferritin) gene and the nucleotide and amino acid sequences of this novel gene were deposited in the GenBank databases with accession numbers DQ207752 and ABA55730, respectively. The entire cDNA of PLF clone was 1109 bp (CDS: 129-653), including a coding nucleotide sequence of 525 bp, a 5' -untranslated region of 128 bp, and a 3'-noncoding region of 456 bp. The 5'-UTR contains a putative iron responsive element (IRE) sequence. Ferritin has an open reading frame encoding a polypeptide of 174 amino acids including a hydrophobic signal peptide of 17 amino acids. The predicted molecular weights of the immature and mature ferritin were calculated to be 20.3 kDa and 18.2 kDa, respectively. The region encoding the mature ferritin was subcloned into the pT7-7 expression vector after PCR amplification using the designed primers and included the initiation and termination codons; the recombinant clones were expressed in E. coli BL21(DE3) or E. coli BL21(DE3)pLysE. SDS-PAGE and western blot analysis showed that a ferritin of approximately 18 kDa (mature form) was produced and that by iron staining in native PAGE, it is likely that the recombinant ferritin is correctly folded and assembled into a homopolymer composed of a single subunit.
허혈성 신손상후 c - fos 및 Epidermal Growth Factor 유전자 발현양상
차대룡(Dae Ryong Cha),이영호(Young Ho Lee),장미경(Mi Kyung Jang),김난희(Nan Hee Kim),구자룡(Ja Ryong Koo),권영주(Young Joo Kwon),조원용(Won Yong Cho),김형규(Hyoung Kyu Kim),김창수(Chang Soo Kim) 대한내과학회 1994 대한내과학회지 Vol.47 No.6
N/A Objectives: Acute renal failure (ARF) is a syndrome that can he broadly defined as rapid deterioration of renal function resulting in the accumulation of nitrogenous wastes such as urea and creatinine. The incidence of ARF will most likely increase in the future as a predictable by-product of the continuous advances in surgical techniques and pharmacotherapy. The severity of renal dysfunction after ARF depends on the exent of the initial renal damage as well as the pace of repair process, and the regeneration of tubular epithelial cell is essential in the recovery of ARF. Recently several studies reveal the importance of specific growth factor such as epidermal growth factor (EGF), transforming growth factor-alpha (TGFα) and the expression of these genes is increased during the recovery stage of ARF. To evaluate the expression of c-fos and EGF genes involved in cellular proliferation during acute ischemic renal failure, author performed the northern and dot hybridization of renal tissue at different reperfusion time in the ischemic ARF rats. Methods: The experimental animals were divided into three groups. Group I (n=3) was control without any procedure, group II (n=3) was sham operation group (bilateral flank incision and decapsulation were made without renal artery clamping), group III (n=15) was ischemic ARF model by right nephrectomy and left renal artery clamping for 40 minutes. In ischemic group (Group III), rats were divided into three subgroups according to reperfusion time such as 1, 24, 72 hours (IIIa, IIIb, IIIc in each). For these studies, the non-ischemic right kidney removed at the time of initial surgery served as a paired control. Becaus of inter-animal variation in mRNA content for specific genes, five rats were included for each time period. In all cases, whole blood were collected for measurement of serum creatinine at each reperfusion time. Total renal RNA was purified from intact whole kidneys by deproteinization with guanidinium isothiocyanate and phenol/chloroform/isoamyl alcohol. After the isolation of RNA, electrophoresis were done in a 1% agarose gel containing 20 mM MOPS, 1 mM EDTA, 5 mM Na acetate PH 7.0 and 2.2M formaldehyde and confirmed the intact RNA by the 18 S and 28 S ribosomal RNA. RNA was transferred to nylon membrane via vaccum transfer and then hybridization were performed at 65℃ with isotope labelled probes for 24hours, Autoradiographs were obtained and quantitated by computer-assisted dual- wave length flying spot scanner (CS-9000) at 530 nm. Results: In the control group, the serum creatinine was 0.9±0.2 mg/dl; in the sham operation group 0.8±0.3 mg/dl; in the ischemic group after 1 hour reperfusion, the serum creatinine was 0.9±0.3 mg/dl; In the ischemic group after 24hous and 72 hours reperfusion, the serum creatinine was 1.9±0.5 mg/dl and 3.6±1.4 mg/dl respectively. The mean serum creatinine level was statistically significant between control and post-ischemic 24, 72 hours reperfusion group (p<0.05). c-fos gene was rapidly induced by renal ischemia with peak after 60 minutes of reflow and 24 hours later c-fos message was markedly decreased. The expression of c-fos gene was. more markedly increased in the more severe ischemic injury. EGF gene expression was markedly decreased after 1 hour of reflow and substantially increased in activity ?2 hours after ischemia. Conclusion: From the above findings, c-fos gene expression is rapidly induced by ischemia and this gene expression is essential in the recovery phase of acute ischemic renal failure. EGF gene expression substantially increased in activity is probablely associated with renal epithelial cell proliferation. Although the specific roles of c-fos and EGF genes in tissue recovery are not known, the expression of these genes may be play an important role in the recovery phase of acute ischemic renal failure.
Unexpected Difficult Intubation Caused by Idiopathic Subglottic Stenosis
( Joo Hyun Jun ),( Mi Hyun Lee ),( Eun Mi Choi ),( Eun Mi Kim ),( Young Ryong Choi ),( Pil Hyun Jeon ),( Seung Hwa Baek ),( Mi Hwa Chung ),( Sung Wook Park ) 경희대학교 경희의료원 2017 慶熙醫學 Vol.32 No.1
A 44-year-old woman was scheduled to undergo an emergency laparoscopic operation for acute appendicitis. Her past medical history included chronic asthma (2 years prior) that was not treated with medication or inhaler. After inducing general anesthesia, a 6.5-mm internal diameter (ID) endotracheal tube (ETT) could not be advanced below the level of the vocal cords because of resistance, and a reattempt with a 6.0-mm ID ETT also failed. Although a laryngeal mask airway was inserted, ventilation was inadequate. The appendectomy was cancelled. Because of the unexpectedly difficult intubation, an otolaryngologist was consulted to examine her larynx, and subglottic stenosis (SGS) of unknown origin was suggested. We present a case of unexpected difficult intubation caused by idiopathic SGS after induction of anesthesia.
인삼의 근 , 엽 및 경(莖)의 사포닌 추출과정중 지용성 용매류의 정제효과
주현규,최강주,김석창,고성용 한국농화학회 1987 Applied Biological Chemistry (Appl Biol Chem) Vol.30 No.4
This study was carried out to investigate effects of fat-soluble solvents on the purification against nan-saponin substances such as chlorophylls and other pigments and on the yields of saponins in separating saponins from ginseng root, leaf and stem. Ginseng root saponins were effectively purified by various fat-soluble solvents while ginseng leaf stem saponins were by chloroform. And alternative extractions of ethyl acetate, ethyl ether, chloroform and benzene there more effective for ginseng leaf stem saponins than that by any single solvent. Contents of crude saponin fractions and total ginsenosides in ginseng leaf were 18.5∼19.5% and 10.8∼1.4%, which were very high compared with 4.6∼5.1% and 2.0∼2.6% in ginseng root or 2.2∼2.5% and 0.63∼0.67% in ginseng stem. Therefore, ginseng leaf is good resources for total saponin or ginsenosides-Rg₁, -Re, -Rc, -Rd, -Rb₂ and -Rf.