Tristetraprolin Overexpression in Gastric Cancer Cells Suppresses PD-L1 Expression and Inhibits Tumor Progression by Enhancing Antitumor Immunity

Jian Guo,Huiheng Qu,Ting Shan,Yigang Chen,Ye Chen,Jiazeng Xia 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.7

The RNA-binding protein tristetraprolin (TTP) binds to adenosine- uridine AU-rich elements in the 3’-untranslated region of messenger RNAs and facilitates rapid degradation of the target mRNAs. Therefore, it regulates the expression of multiple cancer and immunity-associated transcripts. Furthermore, a lack of TTP in cancer cells influences cancer progression and predicts poor survival. Although the functions of TTP on cancer cells have previously been researched, the mechanism of TTP on the interaction between cancer cells with their microenvironment remains undiscovered. In this study, we admed to determine the role of cancer cell TTP during the interaction between tumor and immune cells, specifically regulatory T cells (Tregs). We evaluate the capability of TTP to modulate the antitumor immunity of GC and explored the underlying mechanism. The overexpression of TTP in GC cells dramatically increased peripheral blood mononuclear lymphocyte (PBML) -mediated cytotoxicity against GC cells. Increased cytotoxicity against TTP-overexpressed GC cells by PBMLs was determined by Treg development and infiltration. Surprisingly, we found the stabilization of programmed death-ligand 1 (PD-L1) mRNA was declining while TTP was elevated. The PDL1 protein level was reduced in TTP-abundant GC cells. PD-L1 gas been found to play a pivotal role in Treg development and functional maintenance in immune system. Taken together, our results suggest the overexpression of TTP in GC cells not only affects cell survival and apoptosis but also increases PBMLs -mediated cytotoxicity against GC cells to decelerate tumor progression. Moreover, we identified PD-L1 as a critical TTP-regulated factor that contributes to inhibiting antitumor immunity.

Applications of carbon-based materials in solid phase micro-extraction: a review

Jian Guo,Soo-Jin Park,Long-Yue Meng,Xinghua Jin 한국탄소학회 2017 Carbon Letters Vol.24 No.-

Contradiction

Jian Guo,Lu Fu 한국콘텐츠학회 2013 ICCC International Digital Design Invitation Exhib Vol.2013 No.-

Static aerodynamic force coefficients for an arch bridge girder with two cross section

Jian Guo,Minjun Zhua 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.31 No.3

Aiming at the wind-resistant design of a sea-crossing arch bridge, the static aerodynamic coefficients of its girder (composed of stretches of π-shaped cross-section and box cross-section) were studied by using computational fluid dynamics (CFD) numerical simulation and wind tunnel test. Based on the comparison between numerical simulation, wind tunnel test and specification recommendation, a combined calculation method for the horizontal force coefficient of intermediate and small span bridges is proposed. The results show that the two-dimensional CFD numerical simulations of the individual cross sections are sufficient to meet the accuracy requirements of engineering practice.

Use of multi-hybrid machine learning and deep artificial intelligence in the prediction of compressive strength of concrete containing admixtures

Jian, Guo,Wen, Sun,Wei, Li Techno-Press 2022 Advances in concrete construction Vol.13 No.1

Conventional concrete needs some improvement in the mechanical properties, which can be obtained by different admixtures. However, making concrete samples costume always time and money. In this paper, different types of hybrid algorithms are applied to develop predictive models for forecasting compressive strength (CS) of concretes containing metakaolin (MK) and fly ash (FA). In this regard, three different algorithms have been used, namely multilayer perceptron (MLP), radial basis function (RBF), and support vector machine (SVR), to predict CS of concretes by considering most influencers input variables. These algorithms integrated with the grey wolf optimization (GWO) algorithm to increase the model's accuracy in predicting (GWMLP, GWRBF, and GWSVR). The proposed MLP models were implemented and evaluated in three different layers, wherein each layer, GWO, fitted the best neuron number of the hidden layer. Correspondingly, the key parameters of the SVR model are identified using the GWO method. Also, the optimization algorithm determines the hidden neurons' number and the spread value to set the RBF structure. The results show that the developed models all provide accurate predictions of the CS of concrete incorporating MK and FA with R² larger than 0.9972 and 0.9976 in the learning and testing stage, respectively. Regarding GWMLP models, the GWMLP1 model outperforms other GWMLP networks. All in all, GWSVR has the worst performance with the lowest indices, while the highest score belongs to GWRBF.

Structural Characterization of Microwave-Assisted Solution-Synthesized Strontium-Substituted Hydroxyapatite

Jianguo Liao,Yanqun Li,Xiali Guan,Jingxian Liu,Yongxiang Zhang,Yufen Xie,Zhengpeng Yang,Xingze Duan,Aiguo Zhou,Jiangnan Zhu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.10

Hydroxyapatite and strontium-substituted hydroxyapatite nanoparticles (n-HA and Sr-HA) were prepared by microwave-assisted solution synthesis with aqueous solutions of various Sr/(Sr+Ca) molar ratios ranging from 0% to 15%. The structural properties of the hydroxyapatite powders were investigated by X-ray powder diffraction, Fourier transform infrared spectroscopy and Raman spectra, field emission scanning electron microscopy coupled with energy dispersive X-ray spectrometry and transmission electron microscopy. The results confirmed that strontium ions had been incorporated into the hydroxyapatite lattice. The synthetic n-HA and Sr-HA nanocrystalline consisted of hydroxyapatite crystalline phase with hexagonal structure, and the particle size was 30–40 x 60–70 nm and 40–50 x 70–80 nm, respectively. The calcined HA particle size ranged from about 120 nm to 150 nm, the calcined Sr-HA products were composed of spherical aggregates with a size of about 70–100 nm. The incorporation of Sr ions lead to the formation of vacancies in the crystal structure of the HA. The results indicated that the strontium substitution did not change the crystal structures. More Sr resulted in less calcined crystallites and formed agglomerates owing to the size effect.

Modified Wettability of Micro-structured Steel Surfaces Fabricated by Elliptical Vibration Diamond Cutting

Jianguo Zhang,Andreas Rosenkranz,Junjie Zhang,Jiang Guo,Xiaoqing Li,Xiao Chen,Junfeng Xiao,Jianfeng Xu 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.5

Hardened steel is an important material related to the development of modern industry branches. In order to satisfy the adhesion strength as well as the corrosion and wear resistance under extreme conditions, the modification of the wettability of hardened steel has become an important scientific topic. As micro/nano-structured surfaces play an essential role to induce advanced functional surfaces with wettability control, this contribution aims at presenting the feasibility of micro/nano sculpturing of hardened steels by elliptical vibration diamond cutting. The influence of the fabricated micro/nano structures on the resulting wettability is discussed and related to the contact line density as well as asperity heights. In this regard, it has been verified that a pitch value of 12 μm and structural height of 500 nm are the preferred structural parameters to increase the hydrophobicity of the textured steel specimens.

Structural evaluation of a foldable cable-strut structure for kinematic roofs

Jianguo Cai,Qian Zhang,Yiqun Zhang,Daniel Sang-hoon Lee,Jianfeng Zhao 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.29 No.5

The rapidly decreasing natural resources and the global variation of the climate push us to find intelligent and efficient structural systems to provide more people with fewer resources. This paper proposed a kinematic cable-strut system to realize sustainable structures in responding to changing environmental conditions. At first, the concept of the kinematic system based on crystal-cell pyramid (CP) cable-strut unit was given. Then the deployment of the structure was studied experimentally. After that, the static behaviors in the fully deployed state under the symmetric and asymmetric load cases were investigated. Moreover, the effects of thermal loading and the initial prestress distribution were also discussed. Comparative studies between the proposed structure and other deployable cable-strut system under three times of design load cases were carried out. Finally, the robustness of the system was studied by removal of one passive cable at one time.

Facile Synthesis of Zn1-xCuxO Nanorods with a Very Broad Visible Band

Jianguo Lv,Changlong Liu,Wanbing Gong,Zhenfa Zi,Xiaoshuang Chen,Kai Huang,Feng Liu,Tao Wang,Gang He,Xueping Song,Zhaoqi Sun 대한금속·재료학회 2012 ELECTRONIC MATERIALS LETTERS Vol.8 No.5

Zn1-xCuxO nanorods with different Cu concentrations are prepared by a hydrothermal method. Bent and aggregated nanorods are obtained, which is attributed to centripetal surface tension of the evaporation and coagulation processes of the water film on the ZnO nanorods. The broad visible band consists of one violet, three blue,and one green emission. The violet emission is due to the transition of electrons from zinc interstitial (Zni)levels to the valance band. The three blue emissions may be attributed to the transition from extended Zni levels, which are slightly below the simple Zni level, to the valance band. The change of the green emission may be the result of competition between oxygen vacancies (VO) and zinc vacancies (VZn).

Static analysis of a radially retractable hybrid grid shell in the closed position

Jianguo Cai,Chao Jiang,Xiaowei Deng,Jianfeng Zhao,Yixiang Xu 국제구조공학회 2015 Steel and Composite Structures, An International J Vol.18 No.6

A radially retractable roof structure based on the concept of the hybrid grid shell is proposed in this paper. The single-layer steel trusses of the radially foldable bar structure are diagonally stiffened by cables, which leads to a single-layer lattice shell with triangular mesh. Then comparison between the static behavior between the retractable hybrid grid shell and the corresponding foldable bar shell with quadrangular mesh is discussed. Moreover, the effects of different structural parameters, such as the rise-to-span ratio, the bar cross section area and the pre-stress of the cables, on the structural behaviors are investigated. The results show that prestressed cables can strengthen the foldable bar shell with quadrangular mesh. Higher structural stiffness is anticipated by introducing cables into the hybrid system. When the rise-span ratio is equal to 0.2, where the joint displacement reaches the minimal value, the structure shape of the hyrbid grid shell approaches the reasonable arch axis. The increase of the section of steel bars contributes a lot to the integrity stiffness of the structure. Increasing cable sections would enhance the structure stiffness, but it contributes little to axial forces in structural members. And the level of cable prestress has slight influence on the joint displacements and member forces.