DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9

Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.

Association of Polymorphisms in Stress-Related TNFα and NPY Genes with the Metabolic Syndrome in Han and Hui Ethnic Groups

Bu, De-Yun,Ji, Wen-Wu,Bai, Dan,Zhou, Jian,Li, Hai-Xia,Yang, Hui-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Metabolic syndrome (MS) is a cluster of complicated disorders caused by the interactive influencing factors of heredity and environment, which predisposes to many cnacers. Results from epidemic research indicate that stress is tightly related to the pathogenesis of MS and neoplasia. This paper aims to investigate the association between psychological stress and MS with respect to the tumor necrosis factor alpha (TNF${\alpha}$) and neuropeptide Y (NPY) genes in the Han and Hui ethnic groups. Methods: All subjects for this case-control study matched strict enrollment criteria (nationality, gender and age) and lived in the city of Wu Zhong of Ningxia Province in China. The enrolled group contained 102 matched pairs of Hui ethnic individuals and 98 matched pairs of Han ethnic individuals. Enrolled subjects completed the general Symptom Checklist-90 (SCL-90). The TNF${\alpha}$-308G/A variant and NPYrs16147 polymorphism were detected in case (81 males, 119 females) and control (81 males, 119 females) groups by polymerase chain reaction (PCR) amplification. Results: Nine factors of the SCL-90 were found to be statistically different (p<0.05) between case and control groups. The homozygous mutant genotype (AA) and the mutant allele (A) of the TNF${\alpha}$-308G/A gene were less frequently observed in the control population compared to the case group. The odds ratio (95% confidence interval) in "Allele" for MS was 2.28 (1.47-3.53), p=0.0001, while "OR" was 1.11 (0.83-1.47), p=0.15, for the NPYrs16147 gene polymorphism. Conclusions: Psychological stress has been positively associated with MS. A previous study from our group suggested there were differences in the level of psychological stress between Hui and Han ethnic groups. Furthermore, we found that the stress-related TNF${\alpha}$ gene was associated with MS for both Han and Hui ethnic groups. In contrast, NPY may be a possible contributor to MS and associated cancer for the Han ethnic group.

Effect of Global Warming on Chloride Ion Erosion Risks for Offshore RC Bridges in China

Hui-bing Xie,Yuan-Feng Wang,Jian Gong,Ming-hui Liu,Xiao-Yuan Yang 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.9

Chloride ion penetration, which leads to corrosion of steel reinforcement, is the leading cause of offshore reinforced concrete bridge deterioration. Global warming would accelerate the process of chloride ion penetration and then structural degradation. In China, durability design code for concrete structures has been issued in 2008, and some thresholds of design parameters have been specified to mitigate corrosion in concrete bridge. However, influence of global warming on structure durability was not considered in the issued code. This would overestimate the durability of concrete structures. In this paper, a reliability based method was adopted to evaluate the influence of climate change on the durability of offshore RC bridges considering the acceleration of chloride ion penetration caused by temperature rise. Two climate change scenarios according to IPCC report were considered to define the global warming. A baseline scenario, in which the mean average temperature equals to that of the year 2000, were defined. It is found that the amount of chloride ion on the surface of reinforcing steel bar in the global warming scenarios are 6%-15% higher than that of structure in the baseline scenario. Meanwhile, the probability of corrosion initiation and corrosion damage increased also. In 2100, structures in environmental categories III-C and III-F, designed according to Chinese codes, can no longer adapt the impact of climate change on structure. Comparison of reliability of improved and pre-improved structure was conducted to verify the effectiveness of the proposed improvement measures.

Antioxidant Properties of Green Tea, Oolong Tea, Black Tea and Pu-erh Tea

Jian-Hui Ye,Jing Jin,Yue-Rong Liang 한국차학회 2015 한국차학회지 Vol.- No.S

The antioxidant properties of green tea, oolong tea, black tea and pu-erh tea were studied using DPPH, ABTS and ORAC assays. Green tea had the strongest antioxidant capacities: 201.504 mg trolox/g for DPPH, 676.117 mg trolox/g for ABTS and 1215.029 mg trolox/g for ORAC. For DPPH and ABTS assays, the kinetics of different teas fitted well with second-order exponential decay equations, and single electron transfer reaction was the major reaction process. For ORAC assay, the decay of fluorescence intensity was accurately predicted by Boltzmann sigmoidal equation, and hydrogen atom transfer reaction was the principal reaction. The antioxidant capacities measured by DPPH, ABTS and ORAC assays were positively correlated to the total phenolics contents in teas, among which (-)-epigallocatechin gallate (EGCg) and (-)-epicatechin (EC) had the highest correlation coefficients with the antioxidant capacities of teas.

A random dispersion Schrodinger equation with nonlinear time-dependent loss/gain

Hui Jian,Bin Liu 대한수학회 2017 대한수학회보 Vol.54 No.4

In this paper, the limit behavior of solution for the Schr\"{o}d\-inger equation with random dispersion and time-dependent nonlinear loss/gain: $idu + \frac{1}{\varepsilon} m(\frac{t}{\varepsilon^2})\partial_{xx}udt + |u|^{2\sigma}u dt + i\varepsilon a(t)|u|^{2\sigma_{0}}udt = 0$ is studied. Combining stochastic Strichartz-type estimates with $L^2$ norm estimates, we first derive the global existence for $L^2$ and $H^1$ solution of the stochastic Schr\"{o}dinger equation with white noise dispersion and time-dependent loss/gain:~$idu + \Delta{u} \circ d\beta + |u|^{2\sigma}u dt + ia(t)|u|^{2\sigma_{0}}udt = 0$. Secondly, we prove rigorously the global diffusion-approximation limit of the solution for the former as $\varepsilon\rightarrow0$ in one-dimensional $L^2$ subcritical and critical cases.

Isolation of L-theanine from Tea Solution by Cation Exchange Resin in Batch and Fixed Bed Column

Jian-Hui Ye,Yi-Wen Luo,Hui-Ling Liang,Jian-Liang Lu,Jing Jin,Yue-Rong Liang,Xin-Qiang Zheng,Xian-Yang Luo 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2

L-Theanine, a bioactive compound in tea, was isolated from tea solution using cation exchange resin no.732. The adsorption of L-theanine by cation exchange resin no.732 fit the Langmuir isotherm model and was a monolayer molecular interaction process. Thermodynamic studies revealed that the adsorption of L-theanine by resin no.732 was an exothermic and spontaneous physically driven process. The adsorption capacity was influenced by temperature, initial concentration, and pH. The L-theanine adsorption capacity under conditions at room temperature,pH 4.73, and initial L-theanine concentration 18 g/L was 241.731 ± 3.679 mg/g. The Thomas model was fit to describe the column adsorption data at different flow rates and initial concentrations. The L-theanine adsorbed by resin no.732 could be desorbed by 0.134 mol/L Na2HPO4aqueous solution with a recovery rate of 84.96%. These findings indicate that resin no.732 was a promising material for isolating L-theanine from tea solution.

Prevalence, Risk Factors, and Psychological Effects of Primary Nocturnal Enuresis in Chinese Young Adults

Hui Jie Hu,Zhen Wei Zhang,Yu Liang,Yan Yan Luo,Qi Feng Dou,Cui Ping Song,Hui Min Zhang,Ying Zhao,Guang Run Tian,Ke Zhang,Qiu Fang Mao,Jing Gui Song,Soren Rittig,Jian Guo Wen 대한배뇨장애요실금학회 2021 International Neurourology Journal Vol.25 No.1

Purpose: This study aimed to investigate the prevalence, risk factors, and effects of primary nocturnal enuresis (PNE) on physical and mental health in young adults in mainland China. Methods: An anonymous questionnaire was used to collect information including the sociodemographic characteristics, history of PNE, family history, daytime voiding symptoms, Pittsburgh Sleep Quality Index (PSQI) scores, Self-Esteem Scale (SES), and Self-Rating Depression Scale (SDS). A total of 22,500 university students from 23 provinces and 368 cities in mainland China were included. Results: In total, 21,082 questionnaires were collected, and 20,345 of them qualified for statistical analysis. The PNE prevalence was 1.17%, and the distribution of monosymptomatic nocturnal enuresis (MNE) and nonmonosymptomatic nocturnal enuresis (NMNE) was 66.1% and 33.9%, respectively. In total, 28% of respondents with PNE reported bedwetting daily, 31.6% between 1 and 7 times weekly, and 40.4% between 1 and 4 times monthly; 80% of PNE cases had no history of treatment. The prevalence of PNE in patients with a family history, frequency, urgency, urinary incontinence, and recurrent urinary tract infections was significantly higher than in those without these conditions (P<0.001). PNE was significantly correlated with the PSQI total score (sleep quality) (P=0.011). The SES score was lower and the SDS was higher (P<0.001) in the PNE group than in those without PNE. Conclusions: In mainland China, the PNE prevalence among young adults was found to be high, and PNE had significant effects on physical and mental health. Risk factors included a family history, daytime voiding symptoms, and lack of treatment.

Asp97 is a Crucial Residue Involved in the Ligation of the [Fe4S4] Cluster of IscA from Acidithiobacillus ferrooxidans

( Hui Dan Jiang ),( Xiao Jian Zhang ),( Chen Bing Ai ),( Yuan Dong Liu ),( Jian She Liu ),( Guan Zhou Qiu ),( Jia Zeng ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.6

Simultaneous Single-Position Lateral Lumbar Interbody Fusion Surgery and Unilateral Percutaneous Pedicle Screw Fixation for Spondylolisthesis

Hui Lv,Yu Sheng Yang,Jian Hong Zhou,Yuan Guo,Hui Chen,Fei Luo,Jian Zhong Xu,Zhong Rong Zhang,Ze Hua Zhang 대한척추신경외과학회 2023 Neurospine Vol.20 No.3

Objective: To evaluate the clinical and radiological efficacy of a combine of lateral single screw-rod and unilateral percutaneous pedicle screw fixation (LSUP) for lateral lumbar interbody fusion (LLIF) in the treatment of spondylolisthesis. Methods: Sixty-two consecutive patients with lumbar spondylolisthesis who underwent minimally invasive (MIS)-TLIF with bilateral pedicle screw (BPS) or LLIF-LSUP were retrospectively studied. Segmental lordosis angle (SLA), lumbar lordosis angle (LLA), disc height (DH), slipping percentage, the cross-sectional areas (CSA) of the thecal sac, screw placement accuracy, fusion rate and foraminal height (FH) were used to evaluate radiographic changes postoperatively. Visual analogue scale (VAS) and Oswestry Disability Index (ODI) were used to evaluate the clinical efficacy. Results: Patients who underwent LLIF-LSUP showed shorter operating time, less length of hospital stay and lower blood loss than MIS-TLIF. No statistical difference was found between the 2 groups in screw placement accuracy, overall complications, VAS, and ODI. Compared with MIS-TLIF-BPS, LLIF-LSUP had a significant improvement in sagittal parameters including DH, FH, LLA, and SLA. The CSA of MIS-TLIF-BPS was significantly increased than that of LLIF-LSUP. The fusion rate of LLIF-LSUP was significantly higher than that of MIS-TLIF-BPS at the follow-up of 3 months postoperatively, but there was no statistical difference between the 2 groups at the follow-up of 6 months, 9 months, and 12 months. Conclusion: The overall clinical outcomes and complications of LLIF-LSUP were comparable to that of MIS-TLIF-BPS in this series. Compared with MIS-TLIF-BPS, LLIF-LSUP for lumbar spondylolisthesis represents a significantly shorter operating time, hospital stay and lower blood loss, and demonstrates better radiological outcomes to maintain lumbar lordosis, and reveal an overwhelming superiority in the early fusion rate.

Filters Modeling of Real-Time Electromagnetic Transient Simulation in the Converter Station

Hui, Hui,Liu, Sige,Su, Jian 대한전기학회 2012 The Journal of International Council on Electrical Vol.2 No.4

In this paper, companion model method is applied to establish a basic filter model for electromagnetic transient simulation. Based on the requirements of real-time simulation in the converter station, this paper proposes a simplified filter model and analyzes the speed-up ratio for model optimization. Simulation result shows that under the presupposition of ensuring calculation accuracy, the simplified model can evidently improve the calculation efficiency and play an important role in the realization of real-time electromagnetic transient simulation in the converter station.