Sampled-data H_-/H_~ fault detection and isolation for nonlinear systems in T-S form: An approximate model approach

Jee, S.C.,Lee, H.J. North-Holland 2016 Fuzzy sets and systems Vol.297 No.-

<P>A direct discrete-time design methodology for the sampled-data fault detection and isolation (FDI) problem is proposed for a nonlinear system in Takagi-Sugeno form. The generalized observer scheme is adopted, accompanied by a bank of the sensors' number of observers. A sufficient condition to find observer and residual gains, based on an accessible approximate-rather than unavailable exact-discrete-time model is proposed in terms of matrix inequalities so that it exhibits H-/H-infinity performance and asymptotic stability. An algorithm involving a convex optimization is presented using the cone complementary linearization technique. We show that the FDI observer ensures (modified) H-/H-infinity performance and Lagrange stability, when it is connected to the actual nonlinear system. (C) 2015 Elsevier B. V. All rights reserved.</P>

Pharmacogenetic study of the effects of NK2R G231E G>A and TBX21 H33Q C>G polymorphisms on asthma control with inhaled corticosteroid treatment

Ye, Y-M.,Lee, H-Y.,Kim, S-H.,Jee, Y-K.,Lee, S-K.,Lee, S-H.,Park, H-S. Blackwell Publishing Ltd 2009 Journal of clinical pharmacy and therapeutics Vol.34 No.6

<P>Summary</P><P>Background and Objective: </P><P>Inhaled corticosteroids (ICS) are widely used as maintenance regimens for asthma patients. However, response to ICS shows marked inter-individual variability. Genetic factors have been shown to be potential predictors of responsiveness to ICS. We aimed to evaluate those pharmacogenetic effects on asthma control in further detail.</P><P>Methods: </P><P>Fifty-three mild-to-moderate asthmatics were genotyped for four genetic polymorphisms of four genes: &bgr;2-adrenergic receptor (ADRB2), adenylate cyclase 9 (ADCY9), neurokinin receptor 2 (NK2R) and T-box 21 (TBX21). The principal clinical outcome was the achievement of asthma control, as assessed using the Global Initiative for Asthma (GINA) guidelines. During treatment with ICS, the forced expiratory volume in 1 second (FEV<SUB>1</SUB>), maximal mid-expiratory flow (MMEF) and peak expiratory flow rate (PEFR) were monitored every 4 weeks and twice daily.</P><P>Results: </P><P>Forty-eight of the 53 patients with asthma were in a controlled or partly controlled state after 12 weeks of treatment with ICS, whereas five asthmatics were in an uncontrolled state even after active treatment. Of the four genetic polymorphisms examined, NK2R G231E G>A and TBX21 H33Q C>G were significantly associated with asthma control status (<I>P</I> = 0·041 and <I>P</I> = 0·006). The subjects with wild-type alleles at each polymorphism showed a significant association with the well-controlled or partly controlled state, as compared to those with mutant alleles. At 5–12 weeks after ICS treatment, the NK2R G231E G>A was associated with therapeutic response to ICS, as reflected by improvement in predicted FEV<SUB>1</SUB>%.</P><P>Conclusion: </P><P>Our results suggest that NK2R G231E G>A and TBX21 H33Q C>G are genetic predictors of response to ICS, at least with respect to asthma control status and changes in FEV<SUB>1</SUB>%, in Korean patients with asthma. Further prospective validation of those associations is necessary.</P>

Cloning, characterization and tissue expression of disk abalone (Haliotis discus discus) catalase

Ekanayake, P.M.,De Zoysa, M.,Kang, H.S.,Wan, Q.,Jee, Y.,Lee, Y.H.,Kim, S.J.,Lee, J. Academic Press 2008 FISH AND SHELLFISH IMMUNOLOGY Vol.24 No.3

Catalase is an antioxidant enzyme that plays a significant role in protection against oxidative stress by detoxification of hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>). A gene coding for a putative catalase was isolated from the disk abalone (Haliotis discus discus) cDNA library and denoted as Ab-catalase. The full-length (2864bp) Ab-catalase cDNA contained 1,503bp open reading frame (ORF), encoding 501 amino acid residues with 56kDa predicted molecular weight. The deduced amino acid sequence of Ab-catalase has characteristic features of catalase family such as catalytic site motif (<SUP>61</SUP>FNRERIPERVVHAKGAG<SUP>77</SUP>), heme-ligand signature motif (<SUP>351</SUP>RLYSYSDT<SUP>358</SUP>), NADPH and heme binding residues. Phylogenetic and pairwise identity results indicated that Ab-catalase is more similar to scallop (Chlamys farreri) catalase with 80% amino acid identity except for other reported disk abalone catalase sequences. Constitutive Ab-catalase expression was detected in gill, mantle, gonad, hemocytes, abductor muscle and digestive tract in tissue specific manner. Ab-catalase mRNA was up-regulated in gill and digestive tract tissues for the first 3h post injection of H<SUB>2</SUB>O<SUB>2</SUB>, showing the inducible ability of abalone catalase against oxidative stress generated by H<SUB>2</SUB>O<SUB>2</SUB>. The purified recombinant catalase showed 30,000U/mg enzymatic activity against H<SUB>2</SUB>O<SUB>2</SUB> and biochemical properties of higher thermal stability and broad spectrum of pH. Our results suggest that abalone catalase may play an important role in regulating oxidative stress by scavenging H<SUB>2</SUB>O<SUB>2</SUB>.

Hydrogen sulfide, a gaseous signaling molecule, elongates primary cilia on kidney tubular epithelial cells by activating extracellular signal-regulated kinase

Sang Jun Han,Jee In Kim,Joshua H. Lipschutz,Kwon Moo Park 대한생리학회-대한약리학회 2021 The Korean Journal of Physiology & Pharmacology Vol.25 No.6

Primary cilia on kidney tubular cells play crucial roles in maintaining structure and physiological function. Emerging evidence indicates that the absence of primary cilia, and their length, are associated with kidney diseases. The length of primary cilia in kidney tubular epithelial cells depends, at least in part, on oxidative stress and extracellular signal-regulated kinase 1/2 (ERK) activation. Hydrogen sulfide (H2S) is involved in antioxidant systems and the ERK signaling pathway. Therefore, in this study, we investigated the role of H2S in primary cilia elongation and the downstream pathway. In cultured Madin-Darby Canine Kidney cells, the length of primary cilia gradually increased up to 4 days after the cells were grown to confluent monolayers. In addition, the expression of H2S-producing enzyme increased concomitantly with primary cilia length. Treatment with NaHS, an exogenous H2S donor, accelerated the elongation of primary cilia whereas DL-propargylglycine (a cystathionine γ-lyase inhibitor) and hydroxylamine (a cystathionine-β-synthase inhibitor) delayed their elongation. NaHS treatment increased ERK activation and Sec10 and Arl13b protein expression, both of which are involved in cilia formation and elongation. Treatment with U0126, an ERK inhibitor, delayed elongation of primary cilia and blocked the effect of NaHS-mediated primary cilia elongation and Sec10 and Arl13b upregulation. Finally, we also found that H2S accelerated primary cilia elongation after ischemic kidney injury. These results indicate that H2S lengthens primary cilia through ERK activation and a consequent increase in Sec10 and Arl13b expression, suggesting that H2S and its downstream targets could be novel molecular targets for regulating primary cilia

Transformation of dissolved organic matter by oxidative polymerization with horseradish peroxidase

Jee, S. H.,Kim, Y. J.,Ko, S. O. IWA Publishing 2010 Water Science & Technology Vol.62 No.2

<P>Dissolved organic matter (DOM) has significant influence on the transport and fate of contaminants in multiple phases and it has potential hazard by acting as a precursor of disinfection by-products in water supply. The changes in DOM characteristics, especially by oxidative polymerization might result in different behaviour in the interaction with many contaminants. The aim of this work was to verify the catalytic effects of peroxidase on oxidative polymerization of humic and fulvic substances by examination of the structural characteristics. Transformation of humic acid (HA) and fulvic acid (FA) by oxidative polymerization catalyzed by horseradish peroxidase and hydrogen peroxide were investigated. Size exclusion chromatography, excitation-emission matrices spectra (EEMs), synchronous fluorescence spectra, and infrared spectroscopy was used to evaluate the structural transformation of HA and FA. Molecular weight of HA and FA was continuously changed and their weight-average molecular weight (MWw) reached maximum after 8 h. The MWw of HA and FA were proportionally increased with a dosage of horseradish peroxidase and hydrogen peroxide, indicating that HA and FA was transformed into larger and complex molecules. Spectroscopic results indicated that HA and FA structure contains strong polycyclic aromatic structures with more aromatic rings and a higher degree of conjugation.</P>

바이러스성 설사질환의 역학조사 및 원인체 분석을 통한 예방대책 수립에 관한 연구

지영미,김기순,안정배,천두성,강영화,정윤석,이선화,김운호,윤재득 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Dimeric Ni(II)₂ and polymeric Ni(II)₄Fe(II) type complexes bridged with Cl^- and CN^- ligands: X-ray structures and magnetic properties of a dimeric complex of [(tren)Ni(μ-Cl)₂Ni(tren)](ClO<sub>4</sub

Jee, J.E.,Kwak, C.H. Elsevier 2013 INORGANIC CHEMISTRY COMMUNICATIONS Vol.33 No.-

A dimeric complex of [(tren)Ni(μ-Cl)<SUB>2</SUB>Ni(tren)](ClO<SUB>4</SUB>)<SUB>2</SUB> (1) and a polymeric complex of {[Fe(CN)<SUB>6</SUB>][Ni(tren)]<SUB>2</SUB>[Ni(tren)(H<SUB>2</SUB>O)]<SUB>2</SUB>}Cl<SUB>2</SUB>(ClO<SUB>4</SUB>)<SUB>2</SUB>.4H<SUB>2</SUB>O (2), are isolated from the reaction of potassium ferricyanide, nickel chloride and tren and their X-ray structures and magnetic properties were characterized. Two [Ni(tren)]<SUP>2+</SUP> units are linked through bis μ-Cl ligands forming dimeric structure of 1. In complex 2, there are two kinds of Ni(II) unit, terminal ([Ni(tren)(H<SUB>2</SUB>O)]<SUP>2+</SUP>) and bridged ([Ni(tren)]<SUP>2+</SUP>), and this bridged [Ni(tren)]<SUP>2+</SUP> units are linked with two different Fe(II) ions through μ-NC ligands forming one-dimensional polymeric rectangular chain. 1 shows a weak ferromagnetic coupling between two Ni(II) ions and 2 shows spin crossover in Fe(II) ion.

재생 불량성 빈혈 및 골수 이형성군 환자의 요중 Erythropoietin 활성도의 Bioassay

이봉휘,정문성,지영구,남홍우,송형운,한창순,한치화,박종원,김춘추,--,김창규 대한내과학회 1990 대한내과학회지 Vol.38 No.5

Erythropoietin, a glycoprotein hormone, has an important and well-established role in regulating the developement of RBCs from committed erythropoietic precussor. Its activity can be measured by bioassay or RIA. Recently, we investigated urinary erythropoietin activity in A. A. patients and MDS patients by bioassay and RIA. In bioassay, we used as 3H-thymidine incorperation method to BACB-C mouse spleen cell. Thereafter we compared urinary Epo. acitivity in each disease group and like to find out prognostic significance to be influenced. The results were summarized as follows; 1. The urinary Epo. activities of the A.A. and MDS groups were higher than those of the normal control gorup. and the urinary Epo. activities of the A.A. group showed a marked increase over those of the MDS group (105.5±81.6 vs 1.7±1.5). But the after immune modulation therapyu, urinary Epo. acitivities of the A.A. group markedly decreased to the normal value (105.5±81.6 vs 1.8±1.7, p<0.001). 2. Age and sex differences were not related with urinary Epo. activities in each goup. 3. The hematocrits of the A.A. group did not correlate with any the urinary Epo. acitivities, but an inverse correlation was found in the MDS group. 4. The urinary Epo. activities measured by RIA were higher than those measured by bioassay, but there was a close corrlation between the two methods(R=0. 8, p< 0.001).

각종 갑상선질환에서의 항Thyroglobulin 항체 및 항Microsome 항체의 출현빈도에 관한 연구

이문호,고창순,이홍규,이권전,김병국,최강원,박성회,지제근,이상국 대한핵의학회 1979 핵의학 분자영상 Vol.13 No.1

저자들은 각종 갑상선질환에서 갑상선자가항체의 출현빈도를 조사하여 이들 항체가 갖는 진단적 가치를 관찰하고저, 1978년 4월부터 1979년 4월사이에 서울대학교병원 내과에 내원하였던 각종 갑상선질환환자 108명과 정상인 15명을 대상으로 하여, 감작혈구응집반응을 이용하여 항 thyroglobulin 항체와 microsome 항체를 측정하여 다음과 같은 결론을 얻었다. 1) 정상인 15명에서의 항 thyroglobulin 항체는 모두 음성이었는데, 항 microsome 항체는 1명(6.7%)에서 양성이었다. 각종 갑상선질환환자 108명에서의 결과는 각각 34명(31.5%) 및 40명(37.0%)이 양성이었다. 2) Graves병 환자 25명에서는 항 thyroglobulin 항체는 7명(28.0%)이 양성이었으나 항 microsome 항체는 9명(36.0%)에서 양성이었고, 음성인 군과 양성군 사이에 임상소견 및 검사소견이 특별한 차이를 볼 수 없었다. 3) Hashimoto병 환자는 18명으로 이들은 모두 침생검소견으로 확진된 예들로서, 두가지 항체가 각각 16명(88.9%)과 17명(94.4%)에서 양성이었다. 4) Familial goiter 환자는 7명이었는데 각각 4명(57%) 및 6(85%)명에서 양성이었다. 5) 양성갑성선종 35명에서는 각각 2명(5.7%) 및 3명(8.6%)이 양성의 소견을 보였으며, 갑상선암종 16명에서는 각각 3명(18.8%)이 양성이었다 6) 2명의 아급성갑상선염환자는 두가지가 모두 음성이었던데 반하여, 원발성점액수종환자 1명은 수검검사에서 양성이었다. 7) 1:802 이상의 고력가를 보인 예는 항 thyroglobulin 항체의 경우는 16명(14.8%)이었고, 이중 10명(62.5%)이 Hashimoto병이었으며, 항 microsome항체의 경우는 20명중 13명(65.0%)이 Hashimoto병이었다. 8) Tg Ab와 Mc Ab의 상관계수는 0.76으로 상관관계가 비교적 높으며 Mc Ab의 양성빈도가 약간 높았다. 이상의 결과를 종합하면 감작혈구응집반응은 간편하고 예민하며 신뢰도 및 재현성이 높은 검사로 생각되며 이 방법으로 측정한 항 thyroglobulin 항체 및 항 microsome 항체는 Hashimoto병에서 그 검출빈도가 높고 그 역가도 높아 Hashimoto병의 진단에 유용하다고 사료된다. The authors investigated the incidence of antithyroglobulin antibodies and antibodies and antimicrosomal antibodies measured by tanned red cell hemagglutination method in subjects suffering from various thyroid disorders. 1) In 15 normal patients, neither suffering from any thyroid diseases nor from any other autoimmune disorders, the antithyroglobulin antibodies were all negative, but the antimicrosomal antibody was positive only in one patient(6.7%). 2) The antithyroglobulin antibodies were positive in 31.5%(34 patients) of 108 patients with various thyroid diseases, and the antimicrosomal antibodies were positive in 37.0%(40 patients). 3) of the 25 patients with Graves' diseases, 7 patients(28.0%) showed positive for the antithyroglobulin antibodies, and 9(36.0%) for the antimicrosomal antibodies. There was no definite differences in clinical and thyroid functions between the groups with positive and negative results. 4) Both antibodies were positive in 16(88.9%) and 17(94.4%) patients respectively among 18 patients with Hashimoto's thyroiditis, all of them were diagnosed histologically. 5) Three out of 33 patients with thyroid adenoma showed positive antibodies, and 3 of 16 patients with thyroid carcinoma revealed positive antibodies. 6) TRCH antibodies demonstrated negative results in 2 patients with subacute thyroiditis, but positive in one patient with idiopathic primary myxedema. 7) The number of patiencs with high titers($gt;l:802) was 16 for antithyroglobulin antibody, and 62.5%(10 patients) of which was Hashimoto's thyroiditis. Thirteen(65.0) of 20 patients with high titers($gt;l:802) for antimicrosomal antibody was Hashimoto's thyroiditis. TRCH test is a simple, sensitive method, and has high reliablity and reproducibility. The incidences and titers of antithyroglobulin antibody and antimicrosomal antibody are especially high in Hashimoto's thyroiditis.

High resolution metabolomics to discriminate compounds in serum of male lung cancer patients in South Korea

Pamungkas, Aryo D.,Park, Changyoung,Lee, Sungyong,Jee, Sun Ha,Park, Youngja H. Springer Nature 2016 Respiratory research Vol.17 No.-

<P><B>Background</B></P><P>The cancer death rate escalated during 20th century. In South Korea, lung cancer is expected to contribute 12,736 deaths in men, the highest amount among all cancers. Several risk factors may increase the chance to acquiring lung cancer, with mostly related to exogenous compounds found in cigarette smoke and synthetic manufacturing materials. As the mortality rate of lung cancer increases, deeper understanding is necessary to explore risk factors that may lead to this malignancy. In this regard, this study aims to apply high resolution metabolomics (HRM) using LC-MS to detect significant compounds that might contribute in inducing lung cancer and find the correlation of these compounds to the subjects’ smoking habit.</P><P><B>Methods</B></P><P>The comparison was made between healthy control and lung cancer groups for metabolic differences. Further analyses to determine if these differences are related to tobacco-induced lung cancer (past-smoker control vs. past-smoker lung cancer patients (LCPs) and non-smoker control vs. current-smoker LCPs) were selected. The univariate analysis was performed, including a false discovery rate (FDR) of <I>q</I> = 0.05, to determine the significant metabolites between the analyses. Hierarchical clustering analysis (HCA) was done to discriminate metabolites between the control and case subjects. Selected compounds based on significant <I>m/</I>z features of human serum then experienced MS/MS examination, showing that for many <I>m/z</I>, the patterns of ion dissociation matched with standards. Then, the significant metabolites were identified using Metlin database and features were mapped on the human metabolic pathway mapping tool of the Kyoto Encyclopedia of Genes and Genomes (KEGG).</P><P><B>Results</B></P><P>Using metabolomics-wide association studies, metabolic changes were observed among control group and lung cancer patients. Bisphenol A (211.11, [M + H-H<SUB>2</SUB>O]<SUP>+</SUP>), retinol (287.23, [M + H]<SUP>+</SUP>) and L-proline (116.07, [M + H]<SUP>+</SUP>) were among the significant compounds found to have contributed in the discrimination between these groups, suggesting that these compounds might be related in the development of lung cancer. Retinol has been seen to have a correlation with smoking while both bisphenol A and L-proline were found to be unrelated.</P><P><B>Conclusions</B></P><P>Two potential biomarkers, retinol and L-proline, were identified and these findings may create opportunities for the development of new lung cancer diagnostic tools.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12931-016-0419-3) contains supplementary material, which is available to authorized users.</P>