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      • 염료제거능 곰팡이균주의 동정 및 염료 제거 특성에 관한 연구

        이제혁,황규대,전억한 경희대학교 환경연구소 1995 環境硏究 論文集 Vol.6 No.-

        F-2 isolates cultured on the agar plate containing the dyestuff has formed the clear zone around the strain. The morphology of F-2 was observed with microscope and identified as Penicillium janthinellum(subgenes Furcatum var. Divaricatum). The effects of pH on the absorbace of dyestuff almost not shown except above pH 12 and so the decrease of dye concentration during the incubation of Penjanthinellum is caused by the ability of Penjanthinellum that can remove the dyestuff. The pH and dry cell weight in the incubation of Penjanthinellum have changed after 18 hours and the decrease of the dye concentration in the medium has occured simultaneously. The rates of dye removal were good under the conditions of almost pH ranges except above pH 10 and 0.01g/100㎖ of dye was almost removed in over 3.0g/ℓ of starch concentration. In the case of l%(v/v) inoculum of Penjanthinellum in the 100㎖medium, 0.01g/100㎖ of dyestuff was almost removed in the 20 hours.

      • PEG 분해균주의 분리와 PEG film의 상용성에 관한 연구

        이제혁,정성제,이준열,전억한 경희대학교 부설 식량자원개발연구소 1993 硏究論文集 Vol.14 No.-

        PEG를 sole carbon과 energy source로서 이용하는 미생물을 자연계에서 분리하였고, PEG의 분자량이 높아질수록 그 분해 미생물의 수가 감소하는 것을 확인하였다. 또한, liquid culture로서 PEG농도를 감소시키는 미생물을 선별하였고, 분해율은 PEG 8000이 약 18.8%였으며 PEG 10000은 약 25.4%인 것으로 조사되었다. PEG film의 제조를 위해 EMAA 및 EAA와의 상용성을 적외선 분광(IR) 스펙트럼을 사용하여 조사한 결과, EMAA와 EAA의 카르보닐기와 PEG의 에테르기와의 강한 수소결합이 형성으로 blend film제조시 상용성이 있는 것으로 확인되었다. Several strains capable of degrading PEGs(Polyethylene Glycols)were isolated and investigated for their biodgradation ability of PEGs. Microorganisms screened for the biodegradation studies were those grown on the PEG used as a sole carbon and energy source. It was known that the number of microorganisms decreased when grown on the high molecular weight of PEG(e.g. 20,000). A liquid culture was carried out with such microorgaisms and resulted in the decrease in PEG concentration meaning that PEG was degraded in the reactor. The biodegradability was found to be about 18.8% for PEG-8000 and 25.4% for PEG-10000, respectively. For the manufacture of biodegradable PEG film, EMAA/PEG and EAA/PEG blending ability was investigated with IR spectrum and showed that it was possible to produce blending film.

      • Penicillium janthinellum의 사멸균체를 이용한 염료의 제거

        이제혁,전억한 경희대학교 생명자원과학연구원 1997 硏究論文集 Vol.18 No.-

        사멸균체를 사용한 염료의 흡착율은 배지환경의 pH 감소에 따라 증가하여 pH 2의 경우 염료 Appolocion H-E7B의 흡착ㄹ은 pH6에서 보다 약 60배의 초기 흡착율이 증가하였고, 온도는 높을수록 염료의 흡착율이 증가하여 실험 조건중 가장 높은 온도인 40℃에서 흡착ㄹ이 가장 양호하였다. 종래의 염색폐수 처리 방법으로 처리가 어려운 reactive염료의 흡착율이 우수하였고, 또한 혼합 염료도 흡착율이 양호하여 성상이 불균질한 염색 폐수의 처리에 적용 가능성이 긍정적이었다. 사멸균체에 detergent를 첨가할 경우 염료의 흡착이 저해되는데, 이것은 detergent가 균체의 염료의 결합 부위와 경쟁적으로 결합하여 흡착능을 저하시키는 것으로 사료되며, 각종 금속 ion의 존재시에도 사멸균체에 의한 염료의 흡착에는 거의 영향을 미치지 않아 각종 이온들이 복합적으로 함유된 염색폐수의 처리에도 Pen. janthinellum의 사멸균체가 적합한 것으로 사료된다. It is difficult to remove dyes which are major pollutants in the texile industries. The biosorption was carried out with dead biomass which were prepared by drying and grinding process of harvested microbial cells. The maximum biosorption was obtained with dead cells at pH 2.0 and 40 C. The initial biosorption rates were found to be 0.06, 0.086, 0.079(mg/gㆍmin) for each Apollocion Red H-E7B, Apollofix Red SF-3B, and Apollocion Red H-E3B, respectively. The effects of detergents and ions on the initial biosorption rates were also investigated to gain higher efficiency of dye removal since wastewater contains some ions and detergents. However, detergents added to the modified cells reduced dye biosorption rate and ions used also had no influence on the dye biosorption rates.

      • 염료 분해능 곰팡이의 분리 및 분해능 조사

        이제혁,황규대,전억한 경희대학교 환경연구소 1994 環境硏究 論文集 Vol.5 No.-

        Dyes released into the environment from textile waste water are considered to be a serious polution problem because of the wide spread into environment with a variety of colorants. 12 strains of the dye-degradable fungi were isolated from the soil. Among 12 stains, F-2 showed the highest degradability if dye, was selected for the further dye-degradation study. As a result of the submerged culture of fungi, F-2, in the media containing dye stuff, the specific peak(542㎚) of dye in media was diminishd as cultibation time goes by. The optimum pH and temperature of F-2 were 7-8 and 30℃, respectively. More than 80% of dye in the reactor was removed within 48hts and the agatation speed in the reactor was shown to be optimal at this experoment. It is considered that after waste treatment of the textile waste water by F-2, drained water will not effect th pH of an aquatic condition.

      • Pen.janthinullum에 의한 염료의 흡착 제거 특성

        이제혁,황규대,전억한 慶熙大學校 1996 論文集 Vol.25 No.-

        Dyes released from dyestuff industry and textile industry causes various pollutional problems and poisonous to the organisms. The dye-adsorbable fungus has been isolated and identified as Pen.janthinellum. The optimal temperature and initial pH in the growth of Pen.janthinellum was 30℃ and 7, respectively. It is showed that dye (0.01g/100㎖) is removed completely in the culture broth. When starch as the carbon source is used, the removal of dye was 89% and so starch is available as carbon source for dye removal. And 3g/ℓ above of starch concentration is necessary for remonal of dye(0.01g/100㎖). Apollo Nylon Fast Red S2B., Nylon Fast Red M-E7B., Apollocion Red H-E7B., Apollocion Red H-E3B. was removed about 90.2, 89.3, 87.6, 85.6%, respectively. In the case of detergent addition, the impovement of adsorbability was not shown. It is identified that dead cells have the adsorbability of dye and assumed applications in the waste water treatment.

      • 곰팡이균의 액내배양법에 의한 염료 분해

        이제혁,황규대,전억한 慶熙大學校 1994 論文集 Vol.23 No.-

        Dyes released into the environment from the textile industries are considered to be serious to cause polution problem. 12 strains of the dye-degradable fungi were isolated from the soil and strain showing high activity for biodegradation of dyes used for investigation was selected from 12 strains and coded as F-2. F-2 strain was grown in the medium containing dye as a submerged culture at pH 7∼8 and 30℃ and a dye investigated was degraded at high level(80%) within 48hrs. Comparatively mild agitation speed of impeller(150rpm) was used for the cultivation of fungi F-2.

      • 울진원자력발전소 주변 소나무림의 종조성과 군락 특성

        방제용,전영문,변두원,류병혁,이호준 建國大學校基礎科學硏究所 1995 理學論集 Vol.20 No.-

        울진원자력발전소 주변 소나무림의 종조성과 군락특성을 1994년 3월부터 95년 2월까지 조사한 결과는 다음과 같다. 소나무군락은 상수리나무하위군락과 산철쭉하위군락으로 분류되었으며, 전조사방형구 내에서 출현한 종수는 총 116종으로 상수리나무하위군락과 산철쭉하위군락에서 방형구당 출현종수는 각각 28종, 23종으로 나타났다. 소나무군락의 계층별 평균 수고와 식피율은 상수리나무하위군락에서 교목층이 각각 13.5m와 78.8%, 아교목층이 6m와 37.5%, 관목층이 1.9m와 53.6%, 초본층이 0.4m와 64.4%, 산철쭉하위군락에서는 교목층이 13m와 83.8%, 아교목층이 5.5m와 52.5%, 관목층이 1.9m와 57.5%, 초본층이 0.6m와 69.4%로 나타났다. 피복지수에 의한 계층별 우점순위는 교목층과 아교목층에서 각각 소나무가 8437.5, 1111.3으로 나타났고 관목층에서 진달래(2638.5), 초본층에서 큰기름새(1048.2)가 가장 높게 나타났다. 소나무의 DBH별 분포를 보면 11-15cm급이 상수리나무하위군락과 산철쭉하위군락에서 각각 29.1%(4.2 individuals/a), 26.7%(4.5 individual/a)로 가장 높게 나타났다. 현존식생도에 따른 각 식생형의 분포면적은 소나무림이 133.1k㎡(80.5%), 경작지와 주거지 28.7k㎡(16.2%), 곰솔림 1.8k㎡(1.1%) 순으로 나타났다. 녹지자연도의 등급별 분포는 7등급(이차림)이 70.4%, 2등급(경작지와 주거지) 17.4%, 8등급(이차림) 11.6%, 4등급(벌목지) 0.2%, 6등급(인공조림지) 0.1% 순으로 나타났다. Montreal model에 의한 소나무림의 식물현존량은 3,077,327.8ton으로 산정되었다. The floristic composition and community characteristics of Pinus densiflora forest in vicinity of Ulchin Atomic Power Plant were surveyed from March, 1994 through February, 1995. The results obtained are summarized as follows: The Pinus densiflora community was classified into the subcommunity of Quercus acutissima and Rhododendron yedoence var. poukhanense. The total number of species appearing in the entire surveyed quadrats was 116 sepcies, then 28 and 23 species per quadrat in Quercus acutissima subcommunity and Rhododendron yedoence var. poukhanense subcommunity, respectively. Tree height and vegetation cover by the layer of the Pinus densiflora community were 5m and 78.8% in tree layer, in subtree layer(6m, 37.5%), in shrub layer(1.9m, 53.8%) and in herb layer(0.4m, 64.4%) in Quercus acutissima subcommunity, and in tree layer(13m, 83.8%), in subtree layer(5.5m, 52.5%), in shrub layer(1.9m, 57.5%) and in herb layer(0.6m, 69.4%) in Rhododendron yedoence var. poukhanense subcommunity. The dominance sequences on each stratum determined by the coverage index showed that Pinus densiflora was most dominant in tree layer with 8437.5 and in subtree layer with 1111.3, Rhododendron yedoence var. poukhanense in shrub layer with 2638.5, and Spodiopogon sibiricus in herb layer with 1048.2 value. The highest value of diameter breast height(DBH) distritued in the range of 11~15cm in Quercus acutissima subcommunity(29.1%, 4.2 individuals/a) and Rhododendron yedoence var. poukhanense subcommunity(26.7%, 4.5 individuals/a), respectively. The distribution area of each vegetation type in the actual vegetation map was 80.5% in Pinus densiflora forest, 16.2% in cultivated land and residential section, and 1.1% in Pinus thunbergii forest. The distribution of the degree of green naturality(DGN) was 70.4% in the 7th grade(second forest), 17.4% in the 2nd grade(cultivated land and residential section), 11.6% in the 7th grade(second forest), 0.2% in the 4th grade(cutted area), and 0.1% in the 6th grade(afforestation). It was estimated that phytomass of Pinus densiflora in the study sites, which was calculated by Montreal model, was 3,077,327.8ton.

      • KCI등재
      • 만성 C형 간염 환자에서 페그인터페론 알파2a와 리바비린 병합 치료중 발생한 벨마비 1예

        김일환,장제혁,유충헌,최규남,고정해,김윤정,서광원,김지현,박성재,박은택,이연재,이상혁,설상영 인제대학교 2008 仁濟醫學 Vol.29 No.-

        페그인터페론과 리바비린 병합요법은 만성 C형 간염의 일차 치료법이다. 저자들은 만성 C형 간염 환자에서 페그인터페론 과 리바비린 병합 요법 중에 발생한 벨마비 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 5년 전부터 만성 C형 간염을 앓아온 48세 남자이며, PEG-IFN α-2a 135μgm 피하주사 주1회와 하루 1200㎎의 리바비린을 투여하였다. 치료시작 후 9개월째 환자는 오른쪽 안면의 근력약화를 호소하였으며 벨마비로 진단되었다. 페그인터페론과 리바비린 병합요법을 지속하면서 관찰하였다. 환자의 벨마비는 페그인터페론 치료를 중단하지 않았음에도 3개월후 증상이 회복되고 이후 벨마비 재발 없이 현재 경과관찰 중이다. 만성 C형 간염에서 페그인터페론과 리바비린 병합 요법시 벨마비의 발생 가능성을 염두에 두어야 하겠다. A Case of Bell's Palsy Associated with Combination Therapy of Pegylated Interferon Alfa-2a (PEG-IFN) and Ribavirin for Chronic Hepatitis C Virus Infection Pegylated interferon alfa(PEG-IFN α) and ribavirin therapy is the first line treatment for chronic hepatitis C. Mild complications of the therapy are common, but more serious complications are rare. We report here a case of Bell's palsy that occurred in a patient with chronic hepatitis C virus infection during combination therapy of PEG-IFN α-2a and ribavirin. The patient was 49-year-old man with chronic hepatitis C (genotype 1b) for 8 years. He had compensated liver cirrhosis with splenomegaly. Therapy with PEG-IFN α- 2a 135mcg/week and ribavirin 1200mg/day was initiated. After 9 months of the therapy, the patient showed a loss of muscular tone on the right side of his face. A diagnosis of Bell's palsy was made. The Bell's palsy resolved over 3 months despite continuation of the combination therapy.

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