<i>In vitro</i> and <i>in vivo</i> evaluation of a novel polymer-free everolimus-eluting stent by nitrogen-doped titanium dioxide film deposition

Park, Dae Sung,Bae, In-Ho,Jeong, Myung Ho,Lim, Kyung Seob,Sim, Doo Sun,Hong, Young Joon,Lee, So-Youn,Jang, Eun Jae,Shim, Jae-Won,Park, Jun-Kyu,Lim, Han Chul,Kim, Han Byul Elsevier 2018 Materials Science and Engineering C Vol.91 No.-

<P><B>Abstract</B></P> <P>Inflammation and thrombosis are linked to the use of polymer-based drug-eluting stents (DES). The aim of this study was to develop a polymer-free everolimus (EVL)-eluting stent using nitrogen-doped titanium dioxide (N-TiO<SUB>2</SUB>) and verify its efficacy by <I>in vitro</I> and <I>in vivo</I> assessment in a porcine coronary model. Various analytical approaches such as scanning electron microscopy and atomic force microscopy, electron spectroscopy, Fourier transform infrared spectrometry and contact angle measurement were employed for the characterization. As a part of biocompatibility assessment, platelet adhesion and smooth muscle cell (SMC) proliferation were examined. Bare metal stent (BMS), N-TiO<SUB>2</SUB> stent, everolimus-eluting N-TiO<SUB>2</SUB> (N-TiO<SUB>2</SUB>-EVL) stent, and commercialized EVL-eluting stent (EES) were randomly placed in forty coronary arteries in twenty pigs. After four weeks of implantation, the stents were subjected to histological and quantitative analysis. The N-TiO<SUB>2</SUB> film used in this study was well coated without any cracks or peeling. Surface hydrophilicity (88.8% of angle decrement) could be associated with the decrease in surface roughness post N-TiO<SUB>2</SUB> deposition (37.0%). The platelet adhesion on the N-TiO<SUB>2</SUB> surfaces was less than that on the BMS surface. The proliferation of SMC was suppressed in the N-TiO<SUB>2</SUB>-EVL group (30.2%) but not in the BMS group. In the animal study, the percent area restenosis was significantly decreased in the N-TiO<SUB>2</SUB>-EVL group compared to that in the BMS group. The results (BMS; 47.0 ± 11.00%, N-TiO<SUB>2</SUB>-EVL; 31.7 ± 10.50%, and EES; 29.1 ± 11.21%, <I>n</I> = 10, <I>p</I> < 0.05) were almost at par with those of the commercialized EVL-eluting stent. The introduction of N-TiO<SUB>2</SUB> deposition during fabrication of polymer-free DES may be an efficient accessorial process for preventing in-stent restenosis and thrombosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> N-TiO<SUB>2</SUB> surfaces can help to reduce the platelet adhesion. </LI> <LI> In porcine model, N-TiO<SUB>2</SUB> everolimus decreased in-stent restenosis and fibrin deposition. </LI> </UL> </P>

Association of HLA-DRB1 and -DQB1 Alleles with Susceptibility to IgA Nephropathy in Korean Patients

In Ji Won,Jung Kiwook,Shin Sue,Park Kyoung Un,Lee Hajeong,Song Eun Young 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.1

Background: Associations between IgA nephropathy (IgAN) and HLA-DRB1 and -DQB1 alleles have been reported in several ethnic groups. We investigated the association of HLA-DRB1 and -DQB1 alleles with the predisposition for IgAN and disease progression to end-stage kidney disease (ESKD) in Korean patients. Methods: We analyzed HLA-DRB1 and -DQB1 genotypes in 399 IgAN patients between January 2000 and January 2019 using a LIFECODES sequence-specific oligonucleotide (SSO) typing kit (Immucor, Stamford, CT, USA) or a LABType SSO Typing Test (One Lambda, Canoga Park, CA, USA). Alleles with a significant difference in two-digit resolution were further analyzed using in-house sequence-based typing and sequence-specific primer PCR. As controls, 613 healthy hematopoietic stem cell donors were included. Kidney survival was analyzed in 281 IgAN patients with available clinical and laboratory data using Cox regression analysis. Where needed, P-values were adjusted using Bonferroni correction. Results: The allele frequencies of HLA-DRB1*04:05 (corrected P [Pc]<0.001), -DQB1 *04:01 (Pc=0.048), and -DQB1*03:02 (Pc=0.021) were significantly higher in IgAN patients than in controls, whereas those of HLA-DRB1*07:01, -DRB1*15:01, -DQB1*02:02, and -DQB1*06:02 (Pc<0.001 for all) were significantly lower in IgAN patients than in controls. The allele frequency of HLA-DQB1*05:03 (Pc=0.016) was significantly lower in the ESKD group than in the non-ESKD group; however, there was no significant difference for ESKD progression between these groups. Conclusions: We report novel associations of HLA-DRB1*15:01, DQB1*02:02, -DQB1*03:02, and -DQB1*04:01 with IgAN. Further studies of HLA alleles associated with IgAN progression in a larger cohort and in various ethnic groups are needed.

대전지역 성인의 혈청콜레스테롤 및 중성지방치 수준

윤은영,여인섭 대전대학교 기초과학연구소 1998 自然科學 Vol.9 No.1

본 연구는 대전지역에 거주하는 성인의 혈청지질수준과 생활습관을 조사하기 위하여 대전지역에 거주하며, 건강검진센타를 방문한 성인 250명을 대상으로 1997년 5월-6월에 걸쳐 혈청 콜레스테롤과 중성지방, 사회환경적 요인과 식습관 등을 조사하였다. 조사 결과 Broca 지수의 평균치는 110.2±15.1% 였으며 영양소섭취량은 에너지를 제외하고 모두 권장량의 90% 이상을 섭취하고 있었다. 혈청 총 콜레스테롤 농도는 남자는 203.1±39.4mg/dl, 여자는 202.5±41.3mg/dl로 남녀간 유의적 차이가 없었다. 혈청 LDL-콜레스테롤 농도는 남자 47.2±11.0mg/dl, 여자 53.6±14.1mg/dl 이었다. 중성지방(TG)은 남자는 177.0±131.0mg/dl, 여자는 135.5±83.9mg/dl 이었다. HDL-콜레스테롤은 비만군에서 통계적으로 유의하게 낮았고(p<0.05) 여자의 수치가 남자에 비해 유의하게 (p<0.001) 높은 경향을 보였다. 혈청지질농도간의 상관관계는 TG농도가 높을수록 총콜레스테롤 농도가 증가하였으며, HDL-콜레스테롤농도는 감소하는 경향을 나타내었다. 또한 LPH와 Al는 비만도가 증가할수록 유의적으로 높아졌다(p<0.05, p<0.01). 식습관이 나쁠수록 통계적으로 유의하게 높았다(p<0.05). 대체로 연령이 증가할수록, 수입과 교육수준이 높을수록, 혈청지질 농도가 상승하는 경향을 보였다. The purpose of this study was to investigate serum cholesterol and triglyceride levels of adult in Taejon. This study was conducted with 250 subjects (male 130, female 120) who visited health center in a general hospital from May to June in 1997. The relationship between serum cholesterol and triglyceride levels and some factors were analyzed. Results were summarized as follow ; By Broca index, 26% of the subjects were obese. Mean±SD of Broca index was 110.0±13.7% in male, 10.6±16.6% in female. Serum total cholesterol, LDL-cholesterol, and HDL-cholesterol and Triglyceride levels were 203.1±39.4, 120.5±36.3, 4702±11.0, 177.0±131.0 mg/이 in male3 and 202.5±41.3, 121.7±34.8, 53.6±14.1, 135.5±83.9 mg/이 in female respectively. Total cholesterol, LDL-cholesterol and Triglyceride levels were significantly higher in obese group than non obese groups. But HDL-cholesterol was significantly lower in obese group than non obese groups. And also, the value of LPH and AI in obese group were significantly different among groups. In serum lipid levels by age, total cholesterol, LDL-cholesterol and triglyceride were higher as the subjects were older. And atherogenic index was significantly higher (p<0.05) in groups in the forties, fifties and sixties than group in the twenties. There were significant correlation between serum lipid levels. Above all as the triglyceride level went up, total cholesterol level increased, but HDL-cholesterol level decreased.

Encapsulation of Various Guests by an Anionic In-Metal–Organic Framework Containing Tritopic BTB Ligand: Crystal Structure of Reichardt’s Dye Captured in an In-Metal–Organic Framework

Cho, Eun-Young,Gu, Ja-Min,Choi, In-Hwan,Kim, Wan-Seok,Hwang, Yong-Kyung,Huh, Seong,Kim, Sung-Jin,Kim, Youngmee The American Chemical Society 2014 CRYSTAL GROWTH AND DESIGN Vol.14 No.10

<P>The reaction between 1,3,5-benzenetribenzoic acid (H<SUB>3</SUB>BTB) and In(NO<SUB>3</SUB>)<SUB>3</SUB> hydrate in diethylformamide yielded a new In<SUP>III</SUP>-metal-organic framework, [(CH<SUB>3</SUB>CH<SUB>2</SUB>)<SUB>2</SUB>NH<SUB>2</SUB>]<SUB>3</SUB>[In<SUB>3</SUB>(BTB)<SUB>4</SUB>]·10DEF·14H<SUB>2</SUB>O (<B>I</B>). The countercation and solvent-free doubly interpenetrated <B>I</B> potentially contains 71.0% of solvent accessible void. Although the framework of <B>I</B> was not stable enough to maintain its original structure when the solvent molecules were removed, the as-prepared <B>I</B> was found to be a very good sorbent for acridine orange hydrochloride, a large Reichardt’s dye, and hydrophobic iodine molecule in solution. The as-prepared <B>I</B> exhibited increased uptake amount in the order of Reichardt’s dye > acridine orange hydrochloride > iodine. The largest uptake of the bulky Reichardt’s dye by <B>I</B> could be attributed to the optimized structural fitting of Reichardt’s dye into the large three-dimensional void space of <B>I</B>. The structure of Reichardt’s dye-encapsulated <B>I_RD</B> was unambiguously revealed by X-ray crystallography for the first time.</P><P>Thermal reaction between the tritopic ligand, 1,3,5-benzenetribenzoic acid (H<SUB>3</SUB>BTB), and In(NO<SUB>3</SUB>)<SUB>3</SUB> hydrate in diethylformamide yielded colorless crystals formulated as [(CH<SUB>3</SUB>CH<SUB>2</SUB>)<SUB>2</SUB>NH<SUB>2</SUB>]<SUB>3</SUB>[In<SUB>3</SUB>(BTB)<SUB>4</SUB>]·10DEF·14H<SUB>2</SUB>O (<B>I</B>). The as-prepared <B>I</B> was found to be a good sorbent material for positively charged acridine orange, a large Reichardt’s dye, and hydrophobic iodine molecule in solution.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cgdefu/2014/cgdefu.2014.14.issue-10/cg5005837/production/images/medium/cg-2014-005837_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cg5005837'>ACS Electronic Supporting Info</A></P>

유치원의 부모교육 실태에 관한 조사연구 : 통합 진주시를 중심으로

강인언,황은미 진주여자전문대학 1995 論文集 Vol.18 No.-

The purpose of this study is to investigate the current situations of parent education of kindergarten in Chinju Area. The specific problems of the study are as follows. 1. How is the parent education executed by kindergartens? 2. What is the goal of parent education in kindergarten? 3. What is the content of parent education in kinergarten? 4. What are the patterns of parent education in kinergarten? 5. What is the administration of the parent education in kindergarten? 6. What are the effects of parent education in kindergarten? 7. What are the problems of parent education in kindergarten? 8. How is the degree of parents paticipation? 9. Is there any difference between public kindergartens and private ones in the above problems? For this study, 68 kindergartens in Chinju Area were sampled. The total subjects selected in the study were 47 kindergartens. (28 kindergartens attached to public elementary schools and 19 private kindergartens) The testing instrument used for the study was a questionaire developed by the researchers. The data were analyzed by percentage(%) and Chisquare(χ²) verification. The results of the study are as follows. There were significant differences between public kindergartens and private ones in the execution of parent education, the name of parents meeting, the person determining the membership fee, the inspecting method of kindergarten class, the type and content of interview with parents, the types of correspondence with students home, the existence of home visite programs, the areas and types participating as a assistant teacher in kindergarten, the effects of parent education and the relatioship between kindergarten and students' home.

출력도플러를 이용한 유방 고형 종괴의 검색

김은경,오기근,최병인 대한초음파의학회 1998 ULTRASONOGRAPHY Vol.17 No.1

幼兒文學敎育 實態에 관한 基礎硏究 : 慶南地域 幼兒敎育機關을 中心으로

姜仁彦,金英淑 진주여자전문대학 1988 論文集 Vol.10 No.-

The purpose of this study is to examine the actual condition of the literature education of young children in Gyeong Sang Nam Do, The specific problems of this study are as follows : 1. What is the goal of young childrens's literature education? 2. What is the criteria for the selection of young children's literature books? 3. What is trends of use and interest for media of young children's literature? 1) What is trends of use and interest for fairy tales and creative tales? 2) What is trends of use and interest for picture-tales? 3) What is trends of use and interest for kinds of puppets? 4) Have you ever taken a chance of a puppet-show? 5) Have you ever taken a chance of children's drama? 6) Have you ever used the children's verse in a classroom? 4. How many books of young children's literature do they stock in kindergarten and what is the teacher s viewpoint for these? 5. How is YES or NO of the teacher s experience for a creative activity of young children's literature? 6. For fairy tales : 1) What is the title of fairy tale most used in kindergarten? 2) What is the title of fairy tale which kindergarten-boys are most interested in? 3) What is the title of fairy tale which kindergarten-girls are most interested in? 4) What is the title of fairy tale which kindergarten-teachers are most interested in? 7. What do you want in the class allocated for young children's literature in college? 8. What is the problem in young cildren's literature education? For this study 27 nursery schools, 20 kindergarten attached to elementary schools and 78 private kindergarten in Kyeong Sang Nam Do were randomly sampled. The total subject of the study were 125 teachers. The instrument used for this study was a questionnaire. It was distributed to the teachers and filled out by them. Chi-square was used to analyze by the data obtained from the questionnaire. The results of this study are as follows : 1. For the goal of young children's literature education : 'to be helped creative and expressive activities to literature in various ways' was regarded to be the most important in general. 2. For the criteria for selection of young children's literature books ; 'the contents related a unit was regarded to be the most important in general. 3. For the trends of use interest fot the media of young children s literature ; 'picture-tale' and 'picture-books' are the descending order responded. 4. For books of young children's literature and teacher s viewpoint for those ; '100 books and below them' and 101-200 books' are the descending order responded and answered 'proper' for these. 5. For the YES or No of the teacher's experience for a creative activity of youg children's literature ; 'NO' was regarded to be the most in general. 6. For fairy tales and the title of fairy tale most used in kindergartens ; 'Heungbu-Jean', 'Hokburi-younggam' and 'Hae wa Dali Deun O-nui' are the descending order responded by boys. 'Kongzui and patzui' and 'Baek-seol Princess' are the descending order responded by girls. And 'Hae wa dali Deun O-nui' and 'Hokburi-younggam' are by teachers. 7. For the request to the class allocated for young children's literature in college ; 'the manufacture technique for media of young children's literature and communiate method 'and' the guidance of the creative education to the genre of young children's literature' are the descending order responded by teachers. 8. For the problem in young children's literature education ; 'the problem of manufacture technique for media', 'The difficulty of selection for a literary work and the poor of a subject matter' and 'the poor of teaching materials' are the descending order responded by teachers.

대학 교양과목 수업에서 음악테크놀로지 기반학습 (Music Technology-Based Learning:MTBL)이 감성의 활성화에 미치는 효과

김은진 ( Eun Jin Kim ),강인애 ( In Ae Kang ) 한국감성과학회 2011 감성과학 Vol.14 No.4

지식과 정보가 급변하는 새로운 시대에 디지털 테크놀로지의 활용이 사회, 문화, 교육 전 영역에서 활발히 사용되고 있으며, 예술교육에도 테크놀로지의 활용이라는 새로운 시도를 요구하고 있다. 본 연구는 새로운 교수-학습 방식으로써 "음악 테크놀로지 기반 학습(Music Technology-Based Learning, 이후로 MTBL)"을 제안하고, 이를 수업에 적용하여 학습자들의 감성의 활성화에 대한 효과를 알아보고자 하였다. 본 연구는 2차례의 마인드맵(사전·사후), 매 수업 후 성찰저널 작성, 수업 후 남학생 5명, 여학생 5명의 자발적 참여에 의해 본 연구자를 포함하여 교육공학 전문가 1인과 문화예술교육 전문가 1인, 총 3명과의 심층 인터뷰를 실시하였다. 이와 같은 방법으로 자료를 수집 분석하여 MTBL의 수업 결과 학습자들의 감성의 활성화에 있어 긍정적인 변화를 볼 수 있었다. 사전·사후 마인드맵을 분석에서 보이듯 변화된 단어들의 목록을 통해 학습자들의 감성의 변화를 살펴보았다. 또한 음악 테크놀로지의 활용을 통해 학습자들은 문화예술에 대한 관심과 흥미를 나타내었다. 학습자들은 본 수업에서 음악과 영상을 창작하며 다양한 감성 표현을 보여주었다. 이러한 연구 결과를 바탕으로 MTBL이 예술교육의 새로운 교수-학습 방법으로써 가능성이 있음을 확인 할 수 있었다. Digital Technology actively utilized in all spheres by rapid changes in information and knowledge demands transformations not only in the sociocultural and educational spheres but also specifically in the field of arts education. Digital Technology becomes a challenging factor for the field of arts education. In this study purposed a new teaching-learning approach method in arts education, called "Music Technology-Based Learning"(hereafter, MTBL), which is to, first, take interdisciplinary approaches combining various subjects in the field of arts education. In the case study was conducted to examine the educational effects of the MTBL approach to the liberal arts course in university: mind maps derived from 2 sessions (pre-class and post-class), evaluation sheets regarding self-directed learning and in-depth interviews with ten voluntary learners after the class were used as methods fordata collection. The result of case study shows positive changes in the terms of the degrees of emotional sensibilities of the learners. Moreover, the research confirmed the potential of MTBL as a new teaching and learning methodology in art education.

오차드그래스(Dactylis glomerata L.)의 품종에 따른 종자유래의 캘러스 형성률과, 캘러스 크기 및 식물체 재분화 효율의 비교

배은경,이인애,김기용,이병현,손대영,이효신,정민섭,조진기 Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

Comparison results of callus formation ratio from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency [calculated by following formular; callus formation ratio(%) × regeneration ratio(%)/100]for 27 ochardgrass (Dactylis glomerata L.)varieties imported and Hapsung 2 developed in Korea are as follows; 1. Among ochardgrass varieties showing more than 50% callus formation ratios, the descending order of callus formation ratio after bedding the seed explants for 4 weeks was 93>Sparta>Pizza>Condor>Lidaglo>Glorus>Hapsung2>Frode. 2. The callus sizes after bedding for 4 weeks were in the range of φ 0.43cm~4.2cm in which there was 10 times size difference between the largest one and the smallest one but most of them were between φ2.5cm~4cm. 3. The regeneration ratio from callus among varieties were in the range of 0~36% and descending order of the upper 6 varieties was Plano>Akimidori>Justus>Lidacta>Currie>Hall mark. 4. The regeneration efficiency which is calculated by the ratios of regeneration from seed explant numbers was between 0 to 17.4% among which Justus showed the highest value in the 4-week treatment. 5. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration efficiency were r=0.5765, r=0.6365 and r=0.6246, respectively in 4-week callus and all the correlations were significant on the 1% level. 6. In 6-week callus, the descending order callus formation ratios from seed sxplants for the best 6 varieties was Condor>Sparta>93M>Justus>Potomac>Lidaglo>Frode. 7. The callus sizes formed were between φ1.5~5.7cm in which Sparta, the largest one of φ5.7cm was five times larger than the smallest one. The callus size of the control variety. Hapsung 2 was φ3.8cm, which belonged to larger size. 8. Regeneration ratio showed a great deviation among varieties from 6-week old calli by showing from 0% to 100% in which all the calli were regenerated in Plano while no callus was regenerated in Juno, 9. The range of regeneration effciency was between 0~28% among varieties in which the values from 6-week callus treatment were larger than those from 4-week callus treatment. Especially, the value of Potomac in 6-week was 3 times larger than that in 4-week. 10. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration effciency were r=0.8369, r=0.6683 and r=0.5937, respectively in 6-week callus, and all the correlations were significant on the 1% level.

도시지역 일부 고등학생들의 우울과 불안에 관한 조사 연구

곽은주,송인순,정용준,조영채 韓國保健敎育.健康增進學會 2003 보건교육건강증진학회지 Vol.20 No.2

This study has examined the degree of depression and anxiety among high school students in an urban area and then explored the factors influencing their depression and/or anxiety status. Self-administered questionnaires were offered to 2,381 students from ten high schools in Daejon Metropolitan City. The items for investigation included such factors as various characteristics of school and family life, daily life style, and the degree of depression and anxiety. Based on the study results, the following conclusions were made; 1. The distribution according to the depression degree has shown that 61.9% of students were normal, 32.9% of students had mild depression, 5.0% moderate depression, and 0.3% severe depression. As for anxiety, 40.2% of students were normal, while 19.1% were classified as having anxiety and 40.7% borderline anxiety. 2. As regards to the degree of depression and anxiety, it was greater in girls than boys, in 3rd graders than 1st and 2nd graders, and in the groups who have recieved lower grades in studies, who had poor relations with their friends and a low feeling of satisfaction with school life. In particular, concerning various characteristics of family life, the scores of depression and anxiety were higher in the groups whose financial conditions were poor, whose parents' interests were lower, whose degree of satisfaction with their family and school life was lower, than their counterparts. 3. According to their life styles higher scores of depression and anxiety were found in the groups whose sleeping time was inappropriate, whose breakfasts were skipped, who ate daily snacks, who didn't take regular exercises, and who had poor health habits, compared to their counterparts, respectively. 4. As for the correlation between the degree of depression and its associated variables, the higher scores of depression were in positive correlation with the groups who had lower grades in studies, poor relation with their friends, low feeling of satisfaction with school and home life, low parents' interest, poor subjective condition of health, breakfast skipping, lack of regular exercises, and lower indices of health habits. 5. As for anxiety, the higher scores of anxiety were in the positive correlation with the groups with poor relation with their friends, low feeling of satisfaction with school and family life, poor subjective condition of health, lack of regular exercises, and poor health habits. 6. The influential factors on the depression of students were selected such as subjective condition of health, sex, feeling of satisfaction with family and school life, grades in studies, relation with their friends, presence of regular exercises, degree of interest of parents, sleeping time, cigarette smoking and eating breakfast. 7. The influential factors on the anxiety state of students were selected such as subjective condition of health, feeling of satisfaction with family and school life, sex, sleeping time, regular exercises, cigarette smoking, snacking and relations with their friends. The present study results suggest that girls rather than boys, higher graders than lower ones are more vulnerable to anxiety and depression state, and besides, various characteristics of school and family life, and daily life style can be influential on students' emotional states. Therefore, better management of psychological status of students is thought to require a wide variety of measures to modify the influencing factors and to encourage social support.