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Neupane, Lok Nath,Mehta, Pramod Kumar,Kwon, Joon-Uk,Park, See-Hyoung,Lee, Keun-Hyeung The Royal Society of Chemistry 2019 Organic & Biomolecular Chemistry Vol.17 No.14
<P>The selective ratiometric red-emission detection of Hg<SUP>2+</SUP> ions in aqueous buffered solutions and live cells is still a significant challenge. In the present study, we synthesized a fluorescent probe (1) based on an unnatural peptide receptor containing sulfonamide groups with an aggregation-induced emission and twisted internal charge transfer (TICT)-active fluorophore, cyanostilbene. 1 exhibited a highly selective ratiometric response to Hg<SUP>2+</SUP> among 14 metal ions tested by ratiometric red-emission at 600 nm, with a clear isoemissive point in purely aqueous solution containing 1% DMSO. The ratiometric response for Hg<SUP>2+</SUP> ions was complete within 3 min and the ratiometric responses induced by Hg<SUP>2+</SUP> ions did not suffer considerable interference from the other metal ions. The ratiometric response was complete for less than 7 μM Hg<SUP>2+</SUP> and 1 had a potent binding affinity (7.42 × 10<SUP>−6</SUP> M, <I>R</I><SUP>2</SUP> = 0.98) for Hg<SUP>2+</SUP> and a nanomolar detection limit. 1 detected Hg<SUP>2+</SUP> ions by ratiometric responses in aqueous buffered solutions over a wide range of pH (5.5-11.5). Binding mode studies using TEM, NMR, IR, and a mass spectrometer revealed that the sulfonamide groups of the unnatural peptide receptor played an important role in the complexation of Hg<SUP>2+</SUP> and in the complexation-induced nano-sized aggregates, which resulted in a significant increase in emissions at 600 nm and a decrease in emissions at 535 nm. 1 quantified micro-molar concentrations (0-6 μM) of Hg<SUP>2+</SUP> in tap water and groundwater by ratiometric detection. Furthermore, 1 passed through the lipid membranes of live cells and detected intracellular Hg<SUP>2+</SUP> ions at 2 μM by a ratiometric red-emission change.</P>
Neupane, Lok Nath,Mehta, Pramod Kumar,Oh, Semin,Park, See-Hyoung,Lee, Keun-Hyeung The Royal Society of Chemistry 2018 The Analyst Vol.143 No.21
<P>The development of a fluorescence method for the selective ratiometric detection of Al<SUP>3+</SUP> ions in pure aqueous solutions and live cells is still a significant challenge. In the present study, we synthesized a new type of fluorescent probe using an Al<SUP>3+</SUP>-triggered self-assembly based on the dipeptide receptor and an aggregation-induced emission fluorophore. The fluorescent probe (1) bearing cyanostilbene with excitation by visible light detected Al<SUP>3+</SUP> ions sensitively in pure aqueous buffered solution by ratiometric red-emission at 600 nm. 1 provided a highly selective ratiometric detection of Al<SUP>3+</SUP> among 16 metal ions in aqueous solution. 1 exhibited sensitive ratiometric response to Al<SUP>3+</SUP> in aqueous buffered solutions at pH ranging from 5 to 7.4. The detection limit (145 nM, <I>R</I><SUP>2</SUP> = 0.999) for Al<SUP>3+</SUP> ions in pure aqueous solution was much lower than the maximum allowable level of Al<SUP>3+</SUP> in drinking water demanded by the Environmental Protection Agency (EPA). The probe provided an efficient approach to detect low concentrations of Al<SUP>3+</SUP> in ground water, tap water, and live cells by ratiometric red-emissions at 600 nm. The binding study using dynamic light scattering, NMR, IR, and TEM revealed that the complex between 1 and Al<SUP>3+</SUP> self-assembled to form nanoparticles, resulting in the enhancement of the emission at 600 nm and a concomitant decrease in the emission at 535 nm.</P>
서정탁,이정섭,노형록,최성종 대한슬관절학회 2003 대한슬관절학회지 Vol.15 No.1
목적 : 감염된 인공 슬관절 치환술이 치료에 항생제를 혼합한 시멘트를 삽입하는 2단계 재치환술의 결과를 분석하고자 하였다. 대상 및 방법 : 1988년 3월부터 2001년 3월까지 감염된 인공 슬관절 치환술의 치료로 2단계 재치환술을 받고 최소 2년 이상 추시 관찰이 가능했던 11예를 대상으로 하였으며, 추시 기간은 평균 39.4 (28~60)개월, 수술 당시의 평균 연령은 64.0세였다. 감염의 진단을 위하여 국소 염증 소견, 백혈구 수치, 감별 혈구 계산, 적혈구 침강 속도, C-반응성 단백, 관절 천자 및 술중 동결 절편 (×400) 검사를 시행하였다. 결과의 평가를 위해 Hospital for Special Surgery (HSS)의 슬관절 평가 지수를 사용하였다. 결과 : 술 전 슬관절 운동 범위는 평균 73.4도에서 최종 추시시 평균 90.2도로 슬관절 운동 범위의 증가를 가져왔으며, HSS 기능 평가 점수는 술 전 평균 50.8점에서 최종 추시시 평균 79.1점으로 증가하였다. 최종 추시시에 임상, 검사 소견 및 방사선 소견상 재발은 없었다. 결론 : 감염된 인공 슬관절에서 항생제를 혼합한 시멘트를 삽입하는 2단계 재치환술을 시행하여 최소 2년 이상의 추시 관찰 결과는 좋았다. Purpose: To evaluate the result of treatment of the infected total knee arthroplasty by two-stage revision using antibiotics-impregnated cement. Meterials & Materials : We observed 2 staged revision cases of 11 infected total knee arthroplasty from March 1988 and March 2001. Average follow-up was 39.4(28~60) months. At the time of infection management, the average age was 64.0 years. Local inflammatory finding, WBC with differential count, erythrocyte sedimentation ratio, C-reactive protein, joint aspiration and intraoperative frozen section(×400) were used to diagnose infection. We assessed knee function according to the knee rating system of the Hospital for Special Surgery (HSS). Results : Prior to revision, the average HSS score was 50.8 points, and the average range of motion was 73.4 degrees. At final follow-up, the average HSS score was 79.1 points, and the average range of motion was 90.2 degrees. At the last follow up, there were no sign of recurrence of infection clinically and radiologically. Conclusion : At least 2 years follow up, the results of two-stage revision using antibiotics-impregnated cement were good.