Characterization of Antihypertensive Angiotensin I-Converting Enzyme Inhibitor from Recombinant E. coli

Kim, Jae-Ho,Jeong, Seung-Chan,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

안지오텐신(ACE) 저해제는 항고혈 효과를 갖고 있으므로 오랫동안 고혈압의 예방이나 치료에 이용되어 왔다. 본 연구는 재조합 대장균으로부터 새로운 ACE 저해제를 생산하고 정제하며 나아가 이들이 구조-기능 관P를 규명하기 위해 수행되었다. Saccharomyces cerevisiase의 ACE 저해 펩타이드 유전자를 함유하고 있는 재조합 pGEX-4T-3을 대장균 BL21(DE3)로 형질전환 시켰다. 재조합 pGEX-4T-3을 갖고 있는 대장균 BL21(DE3)로부터 생산된 Glutathione-s 전이효소 (GST) 융합 단백질을 얻어서 그중 ACE저해 펩타이드를 Sephadex G-25 컬럼 크로마토그래피로 정제하였다. 정제된 ACE 저해 펩타이드는 타이로신-아스파틱엑시드-그리신-글리신-발린-패닐알라린-아르기닌-발린-타이로신-트레오닌의 서열을 가진 새로운 decapeptide이었고 ACE에 대하여 경쟁적으로 저해하였다. The angiotensin I-converting enzyme (ACE) inhibitor has anti-hypertensive effects and has long been used as prevention or remedy of hypertension. This study were carried out to produce and purify a new ACE inhibitor from recombinant E. coli and further elucidate its structure-function relationship. Recombinant pGEX-4T-3 containing ACE inhibitory peptide gene of Saccharomyces cerevisiae was transformed into E. coli BL21(DE3). Glutathione-S transferase (GST) fusion protein from E. coli BL21(DE3) harboring the recombinant pGEX-4T-3 was obtained and the ACE inhibitory peptide was purified with Sephadex G-25 column chromatography. The purified ACE inhibitory peptide was a novel decapeptide with sequence Tyr-Asp-Gly-Gly-Val-Phe -Arg-Val-Tyr-Thr which shows very low similarity to the other ACE inhibitory peptide sequence. The purified ACE inhibitor competitively inhibited ACE.

급성 근골격질환에 있어서 피록시캄 근육주사제의 임상적 연구 : An Open, Non-Comparative Trial

윤승호,이광진,김학영,문형석,조성일 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

The authors have conducted a clinical study to evaluate the efficacy and toleration with newly developed piroxicam injectable (I. M.) to thirty-one cases of the patients of musculoskeletal disorders during five months from Oct. 1986 to Feb. 1987. Treatment for the patients was initiated with two amples of piroxicam 20mg I. M. daily for first two days and followed administration one ample of 20mg piroxicam daily for three to seven days. Results were as follows: 1. Out of 31 cases, male were 16 (50.5%), female 15(49.5%), and predominant age group was 20-29 years old 11(35.4%). 2. Major indications were sprained ankle and sprained PIP joint disorders 11(35.7%), 12(38.7 %), respectively. 3. Overall efficacy, more than good was 83.8% by patients and 96.8% by physician. 4. Overall tolerance, more than good was 90.3% by patients and 90.3% by physician (excluded four cases). 5. Side effects were seen in 4 patients such as I case of constipation, 2 cases of abdominal pain and 1 case of vomiting. 6. Conclusion: The piroxicam I. M. was very effective and well tolerated for the treatment of musculoskeletal disorders and side effects were few and generally mild.

한국 성인의 측모두부 수평기준선에 관한 연구

김경호,백형선,김진갑 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.5

교정이나 악교정 수술 환자에서 치료계획 수립과 치료결과의 평가를 위해서 지금까지 많은 수평기준선이 사용되고 있으나 정확성과 재현성 등의 문제가 존재하고 있으며, 더욱 객관적인 수평기준선이 설정될 수 있다면 교정학 분야에서 많은 발전이 기대될 수 있을 것이다. 이에 본 연구에서는 한국 성인의 부정교합군과 성별에 따른 Sella-Nasion(SN) 평면과 Frankfort-Horizontal(FH)평면이 이루는 각도 및 FH평면과 다른 수평면 간의 상관 관계를 조사하기 위하여 먼저 임상검사를 시행하여 총 600여명을 대상으로 측모두부규격 방사선사진을 촬영하였다. 계측결과를 바탕으로 연구대상을 골격성 Ⅰ급 부정교합군 또는 정상교합군(남 50, 여 50), 골격성 Ⅱ급 부정교합군(남 50, 여 65), 골격성 Ⅲ급 부정교합군(남 50, 여50)으로 분류한 후 10개의 항목을 평가하여 다음과 같은 결론을 얻었다. 1. SN 평면과 FH평면이 이루는 각도는 부정교합에 따른 차이는 없었으나 성별 간에는 세 부정교합군 모두에서 차이를 보여 남자는 7.47±2.40˚, 여자는 8.93±2.72˚ 였다. 2. SN 평면 또는 FH 평면과 Mandibular Plane이 이루는 각은 모든 부정교합군에서 여자가 남자보다 컸으며, 골격성 Ⅱ급 부정교합군과 Ⅲ급 부정교합군이 Ⅰ급 부정교합군보다 큰 각도를 나타내었다. 3. FH 평면과 Palatal Plane이 이루는 각은 부정교합군 및 성별에 따른 차이를 보이지 않고 비교적 일정하였다. 4. Gonial angle은 남녀 모두 Ⅲ급 부정교합군이 다른 두 부정교합군보다 큰 각도를 보였다. The purpose of this study was to investigate the angle formed by the Sella-Nasion(SN) plane and Frankort-Horizontal(FH) plane and evaluate the correlation and difference of the FH plane to other horizonatal reference plane. Through this study we hope to present a basis for selecting a horizontal reference plane which can be implemented in cephalometric studies and in surgical orthodontic treatment planning. 600 subjects were chosen following a clinical examination and lateral cephlometric X-rays were taken. According to cephalometric analysis the subjects were classified into 3 groups; Skeletal Class I malocclusion or normal occlusion group(male 50, female 50), Skeletal Class Ⅱ malocclusion group(male 50, female 65) and Skeletal Class Ⅲ malocclusion group(male 50, female 50). The results were as follows. 1. The angle formed by the SN plane and FH plane showed no difference among the malocclusion groups, but there was a significant sex difference. For males the angle measured was 7.47˚±2.40˚ whereas for females it was 8.93˚±2.72˚. 2. The angle formed by the SN plane or FH plane and Mandibular plane was higher in females for all malocclusion groups. This angle in the Skeletal Class I malocclusion group was lower than in the other two groups. 3. There was no difference among the sexes or malocclusion groups considering the angle formed by the FH plane and Palatal plane. 4. The gonial angle in the Skeletal Class Ⅲ malocclusion group was higher than in the Skeletal Class I and Class Ⅱ malocclusion groups in both sexes.

분기관내 혈액의 박동유동특성에 관한 연구

서상호,유상신,노형운 崇實大學校 生産技術硏究所 1995 論文集 Vol.25 No.1

Physiologcal flows of blood and Newtonian fluid in the bifurcated tube are numerically simulated. Velocity and pressure distributions along the axis in the bifurcated tube are obtained for three-dimensional, steady and physiological flows. For the given Reynolds number physiological flow characteristics of blood in the bifurcated tubes are quite different from those of steady flow. Velocity profiles along the bifurcated tube for physiological flows of blood are little higher than those of a Newtonian fluid having the same infinite-shear-rate viscosity as blood at positive inlet velocity, but little lower than those of a Newtonian fluid at negative inlet velocity.

치료시기에 따른 상악골 전방견인효과 : 사춘기전과 사춘기의 비교 A comparison of prepubertal and pubertal patients

김경호,최광철,백형선,이지현 대한치과교정학회 2002 대한치과교정학회지 Vol.32 No.5

상악골 전방견인 장치를 이용한 골격성 Ⅲ급 부정교합 환자의 악정형적 치료에서 치료시기는 치료효과에 지대한 영향을 미칠 수 있으며 이상적인 치료시기는 연구자에 따라 다소의 차이는 있으나 다른 형태의 부정교합보다는 다소 이른 사춘기 이전의 어린 나이라는 것이 선학들의 공통된 의견이었다. 하지만 통계적 자료들을 바탕으로 한 최근의 논문들은 과거 선학들의 의견을 뒷받침 해주는 논문과 그렇지 않은 논문들로 나뉘고 있어 이상적 치료시기에 대한 정립된 의견이 없는 실정이다. 이에 본 연구에서는 119명의 환자들의 수완부골 방사선 사진을 평가하여 골성숙도에 따라 사춘기전군과 사춘기군으로 분류하고 치료전후 측모두부방사선 사진을 분석하여 상악골 전방견인에 대한 치료효과를 비교하였다. 평균 치료기간은 사춘기전군은 6.5개월, 사춘기군은 6.1개월이었으며 치료전후의 측모두부방사선 사진을 분석한 결과 다음과 같은 결론을 얻었다. 1. 상악골의 전방이동양은 사춘기전군과 사춘기군간에 차이에 없었다. 2. 상악골의 하방이동양은 사춘기전군과 사춘기군간에 차이가 없었다. 3. 하악골의 하방이동양과 후하방회전양은 사춘기전군과 사춘기군간에 차이가 없었다. 4. 치성이동양은 사춘기전군과 사춘기군간에 차이가 없었다. 이상의 결과로 사춘기전 뿐 아니라 사춘기에서도 상악골 전방견인에 의해 상악골 성장촉진과 함께 성공적인 악정형 치료가 가능함을 알 수 있었다. Treatment timing is a very important factor to consider in treating skeletal Class Ⅲ patients by means of maxillary protraction. According to the literature maxillary protraction should be carried out at an early age before puberty. However, no scientific data were presented to support this statement and recent studies supported by statistical data showed disagreement concerning the optimum treatment time of maxillary protraction. Therefore, there is no agreement about the optimum treatment time of maxillary protraction. In this study hand-wrist X-rays were taken from 119 patients and subjects were grouped into prepubertal and pubertal group according to their skeletal maturity . Preagreement and posttreatmrnt lateral cephalometric X-rays were taken and treatment effects were compared. The average treatment time was 6.5 months for the prepubertal group and 6.1 months for the pubertal group. The obtained results were as follows : 1. The amount of maxillary forward movement was the same for prepubertal and pubertal group. 2. The amount of maxillary downward movement was the same for prepubertal and pubertal group. 3. The amount of mandibular downward and backward rotational movement was the same for prepubertal and pubertal group. 4. Dental effects were the same for prepubertal and pubertal group.

대퇴골 경부골절에서 인공골두 치환술에 대한 임상적 연구

윤승호,문형석 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

In the treatment of femur neck fracture is very difficult, and many techniques have been known for the trertments. In 1950, Moor designed a Vitallium Prosthesis to replacement for the proximal femur, and Thompson introduces a Vitallium prosthesis in 1954, after then, according to many reports the usual management is hemiarthroplasty using a Moor or Thompson Endoprosthesis, also many reports have apeared of the late results of Endpprosthesis replacements. Fourty cases with fracture of the neck of femur were treated by replacemnet with Endoprosthesis at the Depactment of Orthopedic Surgery, Chungnam National University Hospital, from January 1978 to December of 1982. The results were as follows: 1. The average age of the patients wes 69.4 old, and female was most common. 2. The most common casued of the femur neck fracture were follen-down, or slip-down, an in classification of the femur neck fracture, the transcervical fracture was most common, and by the Garden's classification the type 3 and 4 was most common. 3. We performed Endoprosthesis repalacement frequently for the femur neck fracture with delayed union, avascular necrosis of the head due to fracture of the femur neck, late management of femur neck fracture with delayed and nonunion. 4. We expereneced not so many cases with cemented prosthesis but we expect the further study for these cases. 5. Complications are recognizable to those in old patients. 6. The late results at follow-up with 12 survivers were 75% which had a good excellent results. 7. The motarity ratewhen we follow-up time, it was a little high, it might be due to more old age of patients who treated by Endoprosthesis Replacement.

고효율 충전탑에서 유해가스처리

김장호,김형호,김종수 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.2

In control technology of green house gas CO₂ exhausted, CO₂recovery by chemical absorption technology is recommended. In application to a power station and industrial field with using the absorbent MEA and AMF for carbon dioxide removal. we used packing tower(D=0.l5m, H=1.05m) for experimental apparatus on the chemical absorbtion being used extensively as recovery technique of CO₂. The pressure drop of structured packing Mellapak N. 250Y and random packing 25mm Pall nng was 5.2, 8.5 [mm H₂O/m in the gas capacity factor [kg^(1/2) m^(-1/2) s^(-1)]. Metallic Ralu-Pack 250YC, however, structured packing. has recently being used in petrochemical production plant in general. The decision of tower height depends on the number of transfer unit(NTU) and HTU influence on liquid distribute. If liquid flow is not good material separation, it is bad and usually occurs in the foolding point. In the normal condition, new systematic understanding is given on metal Ralu-Pack 250 YC.

부정교합 분류에 따른 초경시기와 골성숙도에 관한 연구

김경호,백형선,손은수 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.4

부정교합 환자에서 사춘기 성장 가속화시기의 치료는 악안면 골격 부조화의 교정과 안모의 개선에 상당한 영향을 미치므로 사춘기의 성장을 예측하고 성장 잠재력을 평가하는 것은 매우 중요하다. 따라서 교정학에 있어서 개개인의 성장 가속화시기와 연관해서 신체의 성장 발육상태를 평가하는 것은 매우 중요하며 교정치료시 반드시 고려되어야 한다. 이에 본 연구에서는 부정교합 분류에 따라 초경시기에 차이가 있는지와 초경시 골성숙도를 조사하기 위해, I급 부정교합자 64명, II급 부정교합자 51명, III급 부정교합자 38명의 초경 전후 3개월 이내의 수완부골 방사선 사진을 이용하여 골성숙도를 평가한 결과 다음과 같은 결론을 얻었다. 1. 초기 평균 연령은 12.50±1.01세 였다. 2. 각 군별 초경연령은 I급 부정교합군이 12.36±1.04세, II급 부정교합군이 12.81±1.03세, III급 부정교합군이 12.32±0.82세로서, II급 부정교합군이 I급 부정교합군 과 III급 부정교합군에 비해 늦은 초경연령을 보였다. 3. 초경시 수완부 골성숙도는 부정교합에 따른 차이가 없었다. 4. 초경시 수완부 골성숙도는 SMI 7이 45.10%, SMI 8이 27.45%, SMI 9가 10.46%, SMI 6이 7.84%, SMI 10이 7.84%, SMI 5가 1.31% 였다. 5. 초경연령과 수완부 골성숙도는 통계적으로 유의한 상관관계를 보였다 (p<0.05, r=0.25430). In order to correct a maxillofacial-skeletal disharmony sucessfully and achieve a favorable facial profile, orthodontic treatment must begin at pubertal growth spurt. Therefore predicting the pubertal growth pattern and evaluating the growth potential is very important. For an orthodontist, estimating skeletal maturity in relation to one`s personal growth spurt is essential and it must be considered into the treatment. The objective of this study was to find out whether there was a difference in menacheal age among different malocclusion groups and to evaluate the skeletal maturity at menarche. The subjects were 64 Class I malocclusion patients, 51 Class II patients and 38 Class III patients. Skeletal maturity was estimated from handwrist radiographs of these patients. Handwirst radiographs were taken between 3 months before and after the menarche. The results were as follows. 1. The mean chronologic age of menarche was 12.50±1.01 years. 2. For the Class I malocclusion group the mean age of menarche was 12.36±1.04 years, for Class II 12.81±1.03 years and for Class III 12.32±0.82 years. According to these results Class II malocclusion patients started mensturation later than Class I and Class III malocclusion patients. 3. No difference was found considering the skeletal maturity at menarche among the malocclusion groups. 4. The skeletal maturity index at menarche was SMI 7 for 45.10%, SMI 8 for 27.25%, SMI 9 for 10.465, SMI 6 for 7.84%, SMI 10 for 7.84% and SMI 5 for 1.31% patients. 5. Statistically there was a significant correlation between skeletal maturity estimated by handwrist radiographs and menacheal age (p<0.05, r=0.25430).

針葉樹材의 南洋闊葉樹 문틀材 代替 可能性 調査

姜琥陽,張相埴,李華珩 충남대학교 농업과학연구소 1994 농업과학연구 Vol.21 No.2

Since the supply of the Southeast Asian tropical timber decreases and its price steeply increases in the international lumber market, door and window frame manufacturers in this country are eagerly searching for their substitutes. This is the first step to replace Southeast Asian tropical timber by low grade North American softwoods. whose reliable and steady supply is expected, but their appearances are not as good as the tropical timber. In this study, knot distribution on the softwood boards and their warp were examined. The yield and production cost were calculated. The average number of knots on a door frame were 7.2 for Douglas-fir and 3.4 for Western hemlock. Only 11% of the knots of Douglas-fir door frames are larger than 5cm in diameter, while are 35% of the knots of Western hemlock. The deformations of door frames, after 2 months air-drying, were measured with a warp table. The deformation, of Western hemlock door frames were mostly less than 6mm, while those of Douglas-fir distributed in the range of 0.5 and 9.5mm. The yield of Western hemlock is higher than that of Douglas-fir. It can be concluded that the door frames made of the softwoods are compatible with those made of the tropical timber in most aspects except appearance.

빈곤정책과 노인빈곤의 사회적 권리의 비현실화

김상호,김형수 한국사회복지정책학회 2004 사회복지정책 Vol.19 No.-

지난 외환위기 이후 빈곤층이 급증하면서 빈곤문제 해결을 위한 국가의 다양한 개입이 있어 왔다. 하지만 그럼에도 불구하고 노인빈곤이 여전히 폭넓게 존재하고 있으며 인구의 고령화에 따른 빈곤의 노인화에 대한 우려도 적지 않다. 이런 점에서 국가와 빈곤간의 관계, 빈곤의 재생산 등 빈곤의 본질적 측면에 관한 이해가 요구되고 있지만 아쉽게도 이에 대한 연구와 논의는 미흡한 편이다. 본 연구는 국민기초생활보장제도와 국민연금제도를 대상으로 빈곤노인의 사회적 권리의 비현실화 메커니즘과 그 함의를 분석하고 정책적 과제를 모색하고자 하였다. 이를 통해 본 연구는 빈곤정책이 노인빈곤문제 해결에 효과적이지 못한 것은 정책 프로그램이 제대로 작동하지 못 해서라기보다는 광범위한 사회구조적 변화를 충분히 고려차지 못한 곁과로써 그에 따른 사회적 갈등의 심화와 사회통합적 측면이 저해되고 있음을 밝히고 노인빈곤문제 해결과 사회통합을 위한 단기적, 중장기적 방안을 제시하였다. Despite earnest efforts of the government since the economic crisis at the late 1990s, poverty is still prevalent in this country. Poverty problem of the elderly people, particularly, has become worsened to make it a matter of grave concern, as the population is increasingly aging. Although it's true that we have much studied and discussed about the seriousness of the poverty problem of the elderly people, there are still much to be desired in the understanding of such aspects of the problem as the relationship between the state and poverty, reproduction of poverty and true nature of poverty problem. This study is intended to analyze how the Non-realization of poor elderly's social rights are handled in government's policy and identify social implications and political suggestions involved in it. This study found that the perception of the public that government's policy has not been so effective in solving poverty problem of the elderly persons has resulted from its failure to sufficiently reflect wide-ranged changes of social structure rather than improper working of the policy. This study also pointed out the worsening social conflicts and impediments of social integration that have been caused by the failure, and suggested short-term and long-term solutions for the poverty problem of the aged persons.