두경부 편평상피암에서의 MIB-1, p53, bcl-2와 apoptosis의 발현 및 상호 연관성

손용준,한지용,윤형기,장수원,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Squamous cell carcinoma of the head and neck represents approximately 2.0∼4.0% of all malignant tumors, with an etiology closely associated with smoking, drinking and various genetic alterations. The purpose of this study was to investigate the correlation of MIB-1, p53, bcl-2 and apoptosis according to degree of differentitation of squamous cell carcinoma. Methods and Material : forty-eight cases of squamous cell carcinoma of the head and neck were selected for this study. MIB-1, p53, bc1-2 expression rate were studied by iromunogistochemical staining and the number of apoptosis observed by TUNEL method. Results : The expressioins of MIB-1, p53, bcl-2 and apoptosis were found in 56.3%, 50.0%, 14.6%, 85.4%, of 48 cases of squamous cell carcinoma, respectively. The expression of MIB-1, p53 and apoptosis were more higher in poorly differentiated cases than well differentiated cases. MIB-1 expression was related with p53 mutation (p<0.05) and apoptosis rate(p<0.05). Conclusion : From these results. the expressions of MIB-1, bcl-2 and apoptosis were related with degree of differentiation of squamous cell carcinoma and correlation among these parameters.

관광이벤트 연출전략에 관한 연구

원융희,이미혜 용인대학교 산업경영연구소 2003 산업경영논총 Vol.10 No.-

Today, Regions is concern with tourism effects of tourism event. Specially, economic effects is. Because tourism events have for activating their economy and social. Tourism event is important procuctions in order to succeed. Tourism events is to productions, tourists is satisfied and will visit destinations again. Resultly, tourism event has a advantage to enhance the tourism destiantion' image, to attract tourists. This purpose of study is to suggest that is effective production strategies of tourism event. In order to production strategies of tourism event, the major strategies are as follow: to develop a production program formation and production process. Therefore I will research very hard to the dephts of a complex tourist's needs, will try to develop region' traditional and different traits.

관광기업의 전략적 경영연구 : Internal Marketing을 중심으로 focused on Internal Marketing

원융희,이미혜 용인대학교 산업경영연구소 1998 산업경영논총 Vol.3 No.-

Recently, The Strategic Management is very important Tourism companies because the environment of service companies change, The basic premise of the strategic management concept is that strategic planning is the mangerial process of developing and maintaining an optimal ‘fit’ between the deployment of an organization resources and the oppotunities in its changing environment. First all, Internal Marketing of Strategic Management is needed. Internal Marketing is marketing activity for the employees in the organizations. The employee is internal customer and the internal product. From this perspective, The employee have to satifies the needs and motive on a job. The purpose of this study is to provide internal marketing strategies in Tourism Companies and efficient internal marketing are as follows:the subdivision on internal customer' investigation, MBO strategy for different employees, strategic incentive policy, strategic internal support systems, the settle of strategies for tile changes in tourism compaines’environment.

전통문화거리의 체험관광상품 개발전략에 관한 연구

원융희,이미혜 용인대학교 산업경영연구소 2002 산업경영논총 Vol.8 No.-

In those days, Cultural Tourism is emerging touist'need and as alternative tourism Specially, Traditional Cultural Tourism is interesting and is attracting many tourists. The utilizational of Traditional Cultural Tourism resources such as tradtional dress, wedding ceremony, traditional dancing and music, regional festivals and arts contest, traditional life styles have become very popular everyone. Therefore it is important to develop and use cultural assets as experience tourism products. Tourism behavior is a phenomenon as tourists try to find and experience attractions of tourism products. This purpose of study is to suggest that traditional cultural streets as, tourism resources is to develop experience tourist's products. In order to develop experiences tourist's product of traditional cultures are as follows: First, to manage fields of experiences of traditional culture products. and to play in the stage everyday Second, to train experts : guides and interpreters, producers, maketers. Third, to operate puplicity and promotion strateges. In the future study, it is important to develop several experience tourism products of traditional cultural streets. Therefore, I will research very hard to the depths of a complex tourist's needs, will try to develop traditional cultures and will develop several experience tourist's products.

한국전통음식의 새로운 인식과 상품화에 관한 연구

원융희,이미혜 용인대학교 산업경영연구소 2003 산업경영논총 Vol.10 No.-

The purpose of this study is to research the New Recognition and Merchandising of Traditional Foods in Korea. First, this study focused on the relationship between traditional aspects of Korea foods and its meaning in the setting. Second, desire of sight-seeing in increasing sue to extension of sight-seeing experience, so interest about merchandising of food culture necessity of Korean traditional food as sight-seeing goods are being increased. The results of the study as follows : First, Korean foods can be divided in groups by usage, and is distinguished by strong local flavor. Korea regard "traditional foods" as being passed from their ancestors. Second, development of related character in necessary, summarized merchandising way and wrote comment about future studies and these bounds.

관광기업의 무차입경영전략에 관한 연구

원융희,이미혜 용인대학교 산업경영연구소 2005 산업경영논총 Vol.12 No.-

Today, Tourism Enterprises have faced with difficult environment. Therefore tourism enterprises have exerted all possible efforts and strategies of management to raise the real value of tourism enterprises. The purpose of this study was to propose the improvement plan of financial management about the unborrowing management of tourism enterprises. To achieve the purpose of his study, literature research and cases collects, and objective and scientific analysis were theories review and case studies. The unborrowing management strategies of Tourism Enterprises are five strategies : individualization, new business investment, the inner management, a superior financial structure, tourism market control force. The financial management of tourism enterprises have to unborrowing managements and enlarging net worth to enterpirses.

구강 편평세포암종의 Matrix Metalloproteinase(MMP)-1, 2, 3, 9와 Tissue Inhibitors of MMP(TIMP)-1, 2의 발현 양상의 비교 및 상호 연관성

노영서,한지용,김용환,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : nvasiveness of squamous cell carcinoma in oral cavity is associated with poor prognosis, and MMPs have an important role in degradation of extracellular matrix and bawement membrane of vessil walls. Increased expressions of MMPs is related to infiltrative growth and lymph node metastases in human cancers. In normal tissue, there is balanced secretions of MMPs and TIMPs, but impaired balance of MMPs and TIMPs was noted in cancer tissue. The aim of this study is to evaluate the expression rates of MMP-1, 2, 3 and TIMP 1 and 2 in squamous cell carcinoma of the oral cavity, and relationship between MMPs and TIMPs expression and histologic grade and invasion pattern. Methods and Material : Pathologic review of fifty cases of squamous cell carcinoma during a period of Mar. 1994 to Feb. 2000 based on histologic grade and invasion pattim and immunohistochemical stains for MMP-1, 2, 3 and 9 and TIMP-1, 2 were done. Statistical analysis between MMPs and TIMP s expression rate and pathologic parameters and relationship between MMPs and TIMPs were performed. Results : Expression rates of MMP-1, 2, 3 and 9were 32.0%, 38.0%, 22.0% and 20.0%, respectively. MMP-2 expression rate was higher in well differentiated carcinomas and in cases with less-infiltrative pattern. Other MMPs show slightly higher expression rate in well differentiated carcinomas, but there were no statistical significances. There were no significant differences of MMP-1, 2, 3 and 9 expression rates according to invasion pattern. Expression rates of TIMP-1 and TIM-2 were 22.2% and 48.0%, respectively, and there were no significant differences according to histologic grade and invasion pattern. Significant correlations of MMP-1, 2, 3 and 9 expressions except between MMP-2 and MMP-3 were noted, but there were no significant correlations between MMPs and TIMPs expression. Conclusion : Conclusions: In well differentiated and less infiltrative squamous cell carcinomas of the oral cavity, expression rates of MMPs, especially MMP-2, were high. And there were positive correlation between MMPs, but no correlation between MMPs and TIMPs. These findings suggest that MMPs may have a role in tumor developent of a subset of squamous cell carcinoma with less aggressive behavior and MMPs may involve in early stage of tumor progression.

법랑아세포종에서의 p53 및 MIB-1 발현 : 임상 및 병리학적 요인들간의 연관성 Correlation Between Clincopathologic Parameters

이수운,한지용,권창석,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Thirty five patinets' paraffin embedded biops block , which was previously diagnosed ameloblastoma and obtained from oral and maxillofacial surgery dept. of pusan paik hospital and OMS dept of collage dentistry, pusan national university hospital, from January 1994 to march 1999, were performed immunohistochemistry with p-53 and MIB-1. On investigating the expression, correlation between clinical and histologic factor and correlation between prognosis and moleculopathologic property were examined. And we obtained the following results; 1. Age over forty, recurrence rate was 58.5%. 91.4% of the tumor was developed on mandibule, recurrence rate of maxilla area tumor was 66.7% On radiologic and histologic feature, multilocular and follicular type has high recurrence rate but no significance statistical difference was found 2. Expression of p-53 was 25.7%, When underage of forty. location on mandibule, multilocular and plexiform type, the expression of p-53 was somewhat higher and recurred cases of tumor, expression rate was 12.5%. 3. MIB-1 expression rate was 77.1%. When location on mandibule, unilocular, plexiform type tumor and previously recurred case, MIB-1 expression was 87.5% which was somewhat higher than no recurrence case of 74.1%. 4. 33.3.% of positive on MIB-1 have expression of p-53 and all p-53 positive case express MIB-1 positive. All p-53 positive case have high cell proliferation rate. From this result, age and location have closely related with the prognosis of tumor, histologic feature and MIB-1 expression has related with the prognosis of tumor. But p-53 has highly expressed on cell proliferation area, not closely related with the prognosis of tumor.

치성 낭종의 임상-병리학적 소견과 TGF-α 및 TGF-β 발현

한경민,김용환,박진혁,윤형기,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Periapical cyst is a relatively common disease but heterogenous in histogenesis, and inflammatory reaction and fibrosis of varying degree are associated in the cystic wall. The aim of thes study is to evaluate the clinico-pathologic characteristics such as patient's age, ses, location, and the degree of inflammatory reaction and fibrosis of periapical cyst and the role of TGF-α and TGF-β cytokines in the inflammed periapical cyst. Methods and Material : The materials were 43 cases of periapical cyst and clinical information with age, sex, location(maxilla vs mandible, right vs left, region involved) were recorded. Histopathological examination on the degree of inflammatory reaction and fibrosis and immunohistochemical stining for TGF-α and TGF-β were perfomed. Satistical analyses between clinical parameters, the degree of inflammation and fibrosis and expressions for TGF-α and TGF-β were followed. Results : 1. Thirt(69.8%) of 43 cases were older than 20 year-old. and no significant differences of incidence according to patient's sex was noted. In 25(58.1%) out of 43 case. 20(54.1%) out of 37 cases, and 20(48.8%) of 41cases, the cyst was located on the maxilla. in right side and in premolar region respectively. 2. The cysts of developmental and inflammatory origin were 24(55.8%) and 19(44.2%) of 43 cases, respectively and there were no siginificant differences of patient's age, sex, and location of cysts according to the origin of cysts. 3. Fibrosis was more conspicuous in cases older than 20 year-old. (p=0.0179), but no significant difference in the degree of inflammatory reaction according to patient's age and in the degree of inflammatory reaction and fibrosis according to sex and location of the cysts were seen. 4. Inflammatory reaction was slightly more in cysts of inflammatory origin, but no significant defferences in degree of inflammation and fibrosis according to origin of the cysts. 5. The degree of inflammatory reaction and TGF-α and TGF-β expressions showed a tendency of positive correlation(p=0.1563), but there were no statistically significant relationships between the degree of inflammation and fibrosis and TGF-α and TGF-β expressions. 6. In the cysts of developmental origin, TGF-α expression in the epithelial cells was significantly increased than inflammatory origin, but TGF-α and TGF-β expressions in the inflammatory cells and stromal cells showed no significant difference according to the origin Conclusion : The degree of inflammatory reaction and fibrosis of periapical cysts was different according to patient's age and location of the cyst. TGF-α and TGF-β cystokines seemed to be involved in the inflammatory reaction and fibrosis of periapical cyst, however, TGF-α and TGF-β expressions was not parallel to the degree of inflammatory reaction and fibrosis. These results suggest that other cytokines in addition to TGF-α and TGF-β may also be involved in the inflammatory reaction and fibrosis of periapical cyst. Increased TGF-expression in the epithelial cells of the cysts of developmental origin could be a helpful finding to defferentiate the origin of the periapical cyst.

하악 매복 지치의 외과적 발거시 Methylprednisolone의 안면 부종 감소 효과에 대한 임상적 연구

신지훈,박진배,한지용,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : The third molars, expecially mandibular wisdom teeth, are commonly obliquely impacted, and surgical procedure with odontomy and osteotomy is necessary for their extraction. The surgical extracion of impacted wisdom teeth occasionally in duces variable discomfort. Especially, postoperative swelling, pain and limitation of mouth opening determine the degree of discomfort. For the reduction of these complicaions, drug therapy(antihistamine, enzyme, steroid), cole pack and pressure technique have been studied. It is known that the steroids are the most effective. Methods and Meterial : We selected fifty patients in their twenties, who visited Department of Dentistry, Pusan Paik Hospital from March to August in 1998. All of 50 patients has no other medical problems. Total 50 patients were randomly divided to 30 of experimental group and 20 of control group. The experimental group had orally administration of methyprednisolone 5㎎ every 8 hours 2 times preoperatively and 7 times postoperatively. Five points were marked on the patients' face, and the distances between the points were measured just before operation and 48 hours after operation. Results : Methyprednisolone reduces swelling about 50% after surgical extraction of obliquely impacted mandibular third molars with osteotomy and odontomy. The difference in the amount of tissue edema between experimental and control group was significant(p<0.01). However, there was no significant difference according to the operation time. Conclusion : It seems that the use of methylprednisolone is effective to reduce postoperative swelling following surgical extraction of third molars.