가축분뇨를 이용한 SCP 생산 균주의 분리 및 균체 단백질 생산

한석균,고유석,안태영,배동훈 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

질소원으로서 계분을 이용하는 균주를 선별하고 계분배지에서 균체의 생육속도가 다른 균주에 비하여 우수한 균주를 분리하였다. 형태·생리학적 특성을 기초로 하여 yeast의 분류 기준과 비교하여 본 균주를 Candida sp.로 동정하였으며 본 균주를 Candida sp. D116으로 명명하였다. Poultry feces extract medium에서 4% 농도의 glucose 첨가가 균체 생육에 효과적이었다. D116 균주를 액체 발효하여 균체생산능, 요산 그리고 가용성 단백질의 변화를 조사하였다. 그 결과 약 60시간이 경과하면 액체 발효 배지내의 거의 모든 가용성 단백질 및 요산의 감소를 보였으며 균체생육은 약 36시간 배양하였을 때 최고조에 도달하였고 그 후에는 점차 감소하는 경향을 보였다. SCP의 대량생산의 결과 50%의 계분혼합 배지와 30℃의 배양 온도에서 36시간 배양하여 균주의 생육수준이 3.8×10^9 CFU/ml 농도의 균체를 생산하였고 200 L의 배양액중 약 870 g-dw의 균체를 얻었으며 생산된 군체의 조단백질 함량은 67%이었다. Production of Single Cell Protein from Poultry Feces. Suk-kyun Han, You-Suk Go, Tae-Young Ahn and Dong-Hoon Bal^1*. Deparment of Microbioligy, College of Natural Sciences, Dankook Univerity, Cheonan 330-714 and Research Center for Molecular Microbiology, Seoul Nationa University, Seoul 151-742, Korea, ^1Department of Food Engineering. College of Engineering, Dankook University, Cheonan 330-714 and Bioproducts Research Center of Yonsei University, Seoul 120-749, Korea - From the soil collected form provincial area of South Korea, a microorganisms which have been shown good growth in the minimal poultry feces extract medium was isolated. Supplement of glucose to the poultry feces extract medium helped the complete degraded during the microbial growth. Maximum cell growth (3.8×10^9 CFU/ml) obtained at 36 hours of incubation after inoculation. Uric acid was degraded faster in minimal medium that in the glucose complement medium. VFA (volatile fatty acid), which are known as major compounds of poultry feces odor, were almost removed from the minimal poultry feces extract medium. Glucose supplement to the minimal medium enhanced the growth of microbial cells. Addition of 4% of glucose and 4% of neopeptone to the minimal poultry feces extract medium helped the maximal growth of cells.

표면곡률을 이용한 물체의 형상특징추출

조동욱,김지영,박웅규,윤상균,이성석,김태우 西原大學校 1992 西原大學 論文集 Vol.29 No.-

In this paper, a new shape feature extraction method of the objects using surface curvatures is described. First, the values of the H & K are calculated from range data as given input. Then objects are assigned in the form of 8 primitives by the signs of the H & K values. But existing methods have the difficulties with the complex object. Therefore, We propose the method for the complex primitives such as cone ridge and cone valley. Finally, the effectiveness of this paper is demonstrated by several experiment.

全北地域의 人口의 長期 豫測 : A modle of Chonbuk

徐東均,朴正根 全北大學校 1990 論文集 Vol.32 No.-

In this paper the population of Chonbuk province is projected by the survival method. The population projection is the essential factor to establish the long-run developing plan for regional economy. The accuracy of the estimated population is required to accomplish the developing plan efficiently. The estimated population (1990-2001) will be 2,172,015(1990),2,169,948(1995) and 2,247,593(2001) respectively. In the characteristics of population structure, the portions of the young age(0―14) and the old age(60―) to the total population will be over 23% and 13% in 2001. The population structure of Chonbuk will change to the types of the developed country step by step. When the decrease of the young age group and the increase of the old age group are anticipated, the welfare plan for the old age will be gradually required. The rapid decrease of Meon-level population results in the lack of labor forces in rural area. The counterplan to it should be considerated.

여가활동의 기능 향상을 위한 연구

김동건,오노균 忠南大學校體育科學硏究所 1993 體育科學硏究誌 Vol.11 No.1

The purpose of this study was to methods improvement of leisure activity functions, sampling participated in the second class of recreation leader's course from august 10 to 16 in 1993 at Taejon recreation association. Following conclusions were obtained. 1. There was inprovement of scale as competence, control, needs and depth after recreation leader's course. So, became influential needs of leisure education. 2. The competence and control become influential more than needs and depth. 3. There was improvement control among the scale more male than female in the distinction of sex 4. There was improvement competence of objects over a university graduate in particular scholastic ability.

Ascorbic acid 가 골조직세포군의 Phosphatase 에 미치는 영향

김상균,김관식,정동균 대한구강생물학회 1987 International Journal of Oral Biology Vol.11 No.2

Several lines of findings suggest that ascorbic acid might influence the function of osteoblasts although no direct evidences were provided. This study was undertaken to investigate the effect of ascorbic acid on bone cells employing 5 fetal rat calvarial cell populations isolated by sequential enzyme digestion. Fetal rat calvaria were treated 5 times consecutively with enzyme mixture of collagenase, trypsin and EDTA for 10, 10, 10, 20 and 20 minutes. Each bone cell population was primarily cultured for 6-7 days and then the effect of ascorbic acid (10 and 100㎍/ml) on the phosphatase level were determined. The observed results were as follows. 1. Population IV and V had characteristics of osteoblast such as high alkaline phosphatase level and low acid phosphatase. 2. Ascorbic acid decreased the acid phosphatase activity of population IV, regardless its concentration while did not affect other cell populations. 3. Alkaline phosphatase activity of population IV was increased significantly by ascorbic acid. 4. Taken together, these results suggest that ascorbic acid may promote the differentiation of osteoblasts and its effect is restricted in osteoblastic population only, not in other type of bone cells.

한국태권도의 시대적 특징과 갑오경장 이후의 발전과정에 관한 고찰

김동건,오노균 忠南大學校體育科學硏究所 1991 體育科學硏究誌 Vol.9 No.1

The purpose of this study was investigate the beginning, the historical characteristics and the developmental process of Tae Kwon Do after Kabokungjang through sundry records. The following conclusions were obtained: 1. Although there was not enough historical materials to complete the study of beginning of Tae Kwon Do, it can be concluded that Tae Kwon Do in dawn age was used as a means of living in the old stone age. 2. Tae Kwon Do in the age of three Kingdoms was used as an instrument of national defence, in ancient Koryu as an instrument of military discipline, and in Chosun it was used as an instrument for actual fighting or a popular activities in later. 3. After Kabokungjang. Tae Kwon Do has been developed as common people-centered activities from a warrior-centered one, but the militaristic discipline of martial arts like Dang-Su of Japan has been accepted without any critism and prevailed widely until liberation without making a definite distinction between Dang-Su and Kong-Su. After that it was given a name Tae Kwon Do in the early 1960th and it was prevailed as a national sport. 4. Tae Kwon Do in Korea after Kabokungjang subjugated the nature as a traditional martial arts. And then it has been developed as one of the world sports in present time with Cha-Gi in Skill and modernization in type of Game.

디지털 멀티미디어 방송을 위한 8-VSB DTTB 최적 ERP와 안테나 HATT에 관한 연구

최동우,박창균 조선대학교 전자정보통신연구소 2001 電子情報通信硏究所論文誌 Vol.4 No.2

In this paper, we make plans for facilities, the 8-VSB DTV transmission system, installed in the near future, based on presently NTSC TV operating it. In the front paper, estimate transmission power for analog NTSC and digital 8-VSB terrestrial broadcasting, effecting condition of optimal receiving power result from it. It is to estimate optimal receiving condition by using Longley-Rice's Field Strength calculating method that is to guess a profile between transmitting station and many receiving points. The 8-VSB DTV Propagation aims to high-speed transmission rates of 19.39Mbps datum to enable HDTV(High Definition Television) at NTSC 6MHz bandwidth. First of all, an optimalization of field strength between transmission station and receiver must deal with considerable. Because of these reasons, 8-VSB DTV transmission needs effective extension for NTSC TV service coverage. As a result, Transition NTSC VHF(CH4) to DTV UHF(CH34) is expected to much four times transmitting output and then transition NTSC UHF(CH24) to DTV UHF(CH36) is expected to less seven times transmitting output to maintain that equal to NTSC TV is 8-VSB DTV service coverage.

牧丹皮가 Collagen 誘發 關節炎에 미치는 影響

金東赫,宋峰根,金炯均 한국전통의학연구소 1997 한국전통의학지 Vol.7 No.2

류마티스관절염은 활막관절 부위의 만성 염증을 특징으로하며 병이 진행될에 따라 이환된 관절의 연골 및 골과 관절주위 연체조직의 파괴를 초래하는 전신성 자가면역질환이다. 전세계에 걸쳐서 주로 젊은층에서 발병하며 전 인구의 약 2-4%가 이환되는 것으로 알려진 류마티스관절염의 병인에 대하여는 아직도 완전히 규명되지 못하고 있으며,이 질병을 완치 시키거나 질병의 질행을 막을 수 있은 치료법이 정리되지 않은 실정이다. 牧丹皮는 淸熱凉血 活血祛瘀의 효능이 있어 임상적으로 발열,도한,월경불순등 이나 급성충수염 또는 跌打瘀血 등에 응용되어 왔으며 최근에는 진정,최면,진통작용과 항염 및 항알레르기 작용 등이 보고되고 있고 adjuvant 관절염에서 염증반응을 억제하는 것으로 보고되고 있다. 목단피가 콜라겐유발관절염(CIA)에 미치는 영향을 알아보기 위하여 bovine type II collagen을 랫트의 꼬리에 주사하고 목단피를 투여하여 CIA의 유발과정중의 면역학적 변화에 미치는 영향에 대하여 관찰하였다. 그 결과 목단피는 생체내와 생체외에서 담식세포에 의한 탐식능을 증가시켰으며 활막세포에 의한 반응산소중간물질 생성능을 감소시켰다. 또한 목단피는 림프구의 증식을 억제하였고,CD4+세포의 비율을 증가시키고 CD8+세포의 비율을 감소시켰다. 아울러 목단피는 요증 미tric oxide(NO)의 분비를 억제시켰고, 활막세포에서의 NO생성을 약간 증가시키는 효과를 보였으나 고농도의 목단피를 투여할 경우 다시 감소하는 결과를 보였다. 이상의 결과로 볼 때 collagen에 의한 관절염의 진행시 목단피의 투여는 면역반응을 조절하여 ROI와 NO의 생성을 억제하고 탐식능을 증가시켜 외부 항원에 대한 탐식을 효과적으로 하여 관절염의 진행을 완화시킨 것으로 생각되며,따라서 목단피는 류마티스관절염의 치료에 임상적으로 활용할 수 있을 것으로 사료된다. Rheumatoid arthritis(RA) is an autoimmune disorder characterized by a chronic inflammatory, proliferative polyarthritis with joint destruction. The disease occurs throughout the world. Most cases are common in young adult age, and 2-4% of the population are affected with female predominance. However the curative therapy of RA is not yet available, so the development of successful management is required. Moutan Cortex is an herb drug applied for such disease as fever, sweating, menstrual irregularity, acute appendicitis and trauma. Recently it is reported that Moutan Cortex has the antiinflammatory, antimicrobial and antihypertensive actions. It also has analgesic, sedative, and anticonvulsant effects. And it is reported to inhibil swelling of rat's paw and reduce arthritic joint swelling. So as to investigate the effects of Moutan Cortex on collagen-induced arthritis, its water extract was administered in the rat immunized by collagen and the immune responses were measured. The phagocytosis was increased in vivo and in vitro after administration of Moutan Cortex, which was strikingly increased after secondary immunization in vivo. Moutan Cortex decreased lhe reactive oxygen intermediate(ROI) formation and nitric oxide(NO) secretion in urine. While formation of reactive nitrogen intermediate was slightly increased after administration, it showed tendency of decrement in high dose. Moutan Cortex also inhibit lymphocytes proliferation. The ratio of CD4+ T cell was decreased and CD8+ T cell was increased. These results suggest that Moutan Cortex might inhibit arthritic changes by regulating immune response, inhibiting ROI and NO formation and increasing phagocytosis. So Moutan Cortex could be applied for the treatment of RA.

발열체를 가진 2중 수평채널에서의 전열특성에 관한 연구

김동균,박용진,배석태,김시범 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.1

In this study was performed on the characteristics of heat transfer analysis by numerical method in a double horizontal channel with internal heat sources, up and down side walls are adiabatic condition. Pseudo PCB(Printed Circuit Board) with cooling hole its located around heat source. This channel have two heat sources that its condition is uniform heat flux maintenance 5W, upper channel and lower channel inlet velocity 0.2m/sec and outlet open condition. Parameter is cooling hole's location on the plate(PCB) and increasing heat flux(10W,15W) for one case.

골흡수 기전에 관한 연구 : 파골세포의 활성화 기전 MECHANISM OF OSTEOCLAST ACTIVATION

정동균,고재승,김관식,김각균,민병무,김세원 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.1

Although the osteoclast has long been recognized to be the cell responsible for bone resorption, little is known of the mechanisms by which its activity is controlled. Recently, it has been suggested that osteoblasts ─ the bone-forming cells ─ seem to be the target cells of PTH, the bone-resorbing hormone, and mediate osteoclastic bone resorption by producing the coupling factor(s). Because bone tissue consists of several types of cells, isolation of distinct bone cell populations is prerequisite for studying the mechanism of bone resorption in cellular level. This experiment was performed ⅰ) to isolate the metabolically distinct bone cell populations from fetal rat calvaria by sequential enzyme digestion and biochemical characterization and ⅱ) to identify the factor(s) produced by osteoblast that stimulate resorption employing organ culture of bone. Calvaria from rat fetus at 19 day of gestation, were sequentially digested by enzyme solution consisted of collagenase, trypsin and EDTA for 10 (population I), 10(II), 10(III), 20(IV) and 20 minutes (V). Each bone cell population was primarily cultured for 6-7 days and effects of PTH, calcitonin and PGE_2 on acid and alkaline phosphatase activity and cAMP level were determined. Basal level of acid phosphatase in populations released early were higher than late population. In contrast, basal level of alkaline phosphatase was reversed. PTH(0.4 unit/ml) increased the acid phosphatase activity only in population I with no effect on alkaline phosphatase. Calcitonin(150ng/ml) had no effect on acid and alkaline phosphatase activity in all bone cell populations. cAMP level of population IV and V were increased by PTH significantly while CT had no effect in all bone cell populations at all. PGE_2 increased cAMP in all populations, the acid phosphatase activity in population I and alkaline phosphatase activity in population IV and V. Taken together, these results indicate that population IV and V express typical osteoblastic phenotype while population I revealed some characteristics of osteoclast. Bone cell population IV and V were incubated with fresh MEM or MEM containing 0.4U/ml PTH for 2 hours. After 2 hour-incubation, both the control-conditioned media(control-CM) or PTH-conditioned media(PTH-CM) were collected. Both conditioned media were lyophyllized and redissolved as 2 fold concentrate. Ulnae and radii were removed from 19-day old fetal rats, prelabelled by subcutaneous injection of 200μCi ^45CaCl_2 into their mothers on the 17th day of gestation. After 24 hours, media was changed with fresh BGJb media or BGJb media containing 300μl of control-CM or PTH-CM and cultured for 5 days. Effects of control-CM or PTH-CM were observed by the ratios of %-release of ^45Ca between paired control and experimental group. Control-CM obtained from population IV and V had no or very little effect on bone resorption but PTH-CM obtained from population IV and V increased the ^45Ca release significantly after 3 and 5 days of culture. This result provides the evidence indicating that osteoblastic cells mediate osteoclastic bone resorption stimulated by PTH.