알코올 의존과 후보 유전자들간의 연관성 연구

정인원,김헌,홍주봉,지경환,이규영 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.6

연구목적 : 알코올 대사와 중추신경계내의 알코올 작용부위와 관련한 일부 후보유전자들이 알코올 의존과 관련이 있는지를 확인하고자 하였다. 방 법 : DSM-IV로 진단된 알코올 의존 환자 128명과 정상 대조군 128명을 대상으로 후보 유전자들의 다형성을 중합효소 연쇄반응과 제한효소처리법을 이용하여 동정하였다. 여기서 분리된 대립유전자와 유전자형에 따른 빈도의 차이를 유전자별로 각각 비교하였으며, 알코올 의존에 작용하는 서로 다른 유전자들에 의한 영향력을 배제하기 위하여 logistic regression 분석을 적용하였다. 결 과 : 알코올 의존군에서는 ALDH_2 유전자의 NN(wild type)형이 정상대조군에 비하여 유의하게 많았다(chi-square test, p<0.001). logistic regression analysis를 통하여 다른 유전자들에 의한 영향을 보정한 상태에서 ALDH_2가 NN(wild type)인 경우 다른 아형에 비하여 알코올 의존에 대한 odds ratio는 130.312이며 그 신뢰구간은 (17.22, 986.43)이었다. 한편, ASA는 N-glycosylation 부위에 변이형 대립유전자를 갖고 있는 경우(AG or GG)는 wild type에 비하여 알코올 의존에 대한 위험도는 2.344배이며 그 95% 신뢰구간은 (1.128, 4.871)이었다. 결 론 : 알코올 의존과 ALDH_2 유전자간에는 밀접한 연관성이 확인되었다. 또한 다른 유전자들은 개별 연관분석에서 의미있는 연관성이 관찰되지는 않았지만 logistic regression analysis를 통하여 N-glycosylation 부위에 다형성이 있는 ASA 유전자가 알코올 의존과 관련이 있음을 시사하였다. Objectives : This study was to explore the association between alcohol dependence and five candidate genes related to the metabolism of alcohol and the enzymes of the suspected sites in CNS. Methods : The genotype and allele frequencies of five candidate genes in 128 male hospitalized patients who met DSM-IV criteria for alcohol dependence were compared with 128 age-matched healthy male control subjects using polymerase chain reaction and restriction fragment length polymorphism. A logistic regression analysis was applied in order to exclude the reciprocal interactions among five candidate genes. Results : The NN genotype frequency of the ALDH_2 gene was significantly higher in alcoholic patients than in control subjects(chi-square test, p<0.001). No difference in frequency was found in the other four genes. In a logistic regression analysis, the odds ratio for alcohol dependence in the NN genotype of the ALDH_2 gene and AG or GG genotypes of the N glycosylation site on the ASA gene were 130.312(95% confidence interval, 17.22-986.43) and 2.344(95% confidence interval. 1.128-4.871), respectively. Conclusion : The result reiterates the association of the ALDH_2 gene polyporphism and the alcohol dependence. Logistic regression analysis additionally suggested that the N-glycosylation site on the ASA gene was associated with alcohol dependence.

항혈청 투여에 따른 돼지 전염성 위장염 예방효과 : Ⅰ. 혈청학적 결과, RT-PCR 검사, 형광항체검사 Ⅰ. Serological Results, Rt-PCR for Fecal and Small Intestin, FA Test

지영철,한정희,권혁무,한태욱,정현규,박봉균 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against transmissible gastroenteritis virus (TGEV) infection in piglets by administration of the TGEV antiserum orally at 5 hrs, 24 hrs and 36 hrs after birth. five piglets adiminstered the antiserum were experimentally infected with TGEV at four-day-old. Control group were four piglets infected with TGEV only. Serum antibody titers against TGEV were examined by serum neutralization (SN) test, dectection for TGEV or TGEV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the TGEV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of TGEV in feces and small intestines by RT-PCR were 24.5% and 20.0% in TGEV antiserum treated group and 44.0% and 75.0% in control group, respectively. 3. The detection rate of TGEV antigen in the small intestine by IFA were 26.7% in TGEV antiserum treated group and 75.0% in control group, respectively. It was concluded that oral administration of antiserum against TGEV to piglets was effective in preventing TGEV infection.

GiemsaC-分染에 의한 리기다소나무, 소나문 및 곰솔의 核型

池充源,徐奉甫 慶北大學校 自然科學大學 1986 自然科學論文集 Vol.4 No.-

C-banding karyotypes of Pinus rigida, P. densiflora and P. thunbergii were investigated. Somatic chromosome number of three species was found to be 2n=24, and twelve pairs of homologous chromosome were classified on the basis of the number and size of bands, secondary constriction, total length and arm ratio. The results obtained under this study are summarized as follows; chromosome complements in three species were all metacentric, and the interspecific difference was difficult to identify in the chromosome morphology. The bands on chromosomes were generally located in the centromere, secondary constriction, and intercalary regions of the long and short arms. The bands were revealed in the all centromeric regions except for a pair of E chromosome in P. rigida. The number of bands revealed on each chromosome was one or two.

항혈청 투여에 따른 돼지 전염성 설사병 예방효과 : Ⅰ.혈청학적 결과, RT-PCR 검사, 형광항체검사 Ⅱ.Serological Results, Rt-PCR for Fecal and Small Intestin, FA Test

지영철,한정희,권혁무,한태욱,정현규,박봉균 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (REDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Serum antibody titers aginst PEDV were examined by serum neutralization (SN) test, dectection for PEDV or PEDV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the PEDV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of PEDV in feces and small intestines by RT-PCR were 26.2% and 16.7% in PEDV antiserum treated group and 48.1% and 75.0% in control group, respectively. 3. The detection rate of PEDV antigen in the small intestine by IFA were 0% in PEDV antiserum treated group and 50.0% in control group, respectively. It was concluded that oral administration of antiserum against PEDV to piglets was effective in preventing PEDV infection.

[논문]베이스플레이트의 비선형 거동에 관한 연구

김재봉,이치형,윤성기 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

본 연구에서는 편심하중이 작용하는 철골 주각부의 모멘트-회전거동에 대한 실험적인 결과와 ANSYS 프로그램을 이용하여 베이스플레이트의 비선형 거동특성을 파악하는데 목적이 있다. 또한,베이스플레이트의 비선형 거동에 영향을 미치는 여러 변수들을 고려하였으며, 실험과 유한요소 해석을 통하여 비교하였다. 그 결과 주각부의 회전거동은 반강접성을 나타내며 베이스플레이트의 설계와 골조의 거동해석에 중요한 영향을 미치는 것으로 판단된다.

南北交流·協力附屬合意書와 GATT와의 國際法上 問題點

池奉道 명지대학교 사회과학연구소 1994 사회과학논총 Vol.9 No.-

日本의 獨島領有權主張에 관한 法的 批判과 韓日間 排他的 經濟水域의 境界劃定

池奉道 明知大學校 附設 日本問題硏究所 1997 日本硏究 Vol.5 No.-

CDMA 시스템의 전송력 조절 문제의 해와 알고리즘

정치봉 순천향대학교 기초과학연구소 1998 순천향자연과학연구 논문집 Vol.4 No.2

In Cellular wireless communication systems, transmitted power is regulated to provide each user an acceptable connection while limiting the interference seen by other users. Previous work has focused on maximizing the minimum carrier to interference ratio (CIR) or attaining a common CIR over all ratio links. However, previous work has assumed the assignment of mobiles to base stations is known and fixed. In the work, we integrate power control and base station assignment. In the context of a CDMA system, we consider the minimization of the total transmitted uplink power subject to maintaining an individual target CIR for each mobile. This minimization occurs over the set of power vectors and base station assignments. We show that this problem has special structure and identify synchronous and asynchronous distributed algorithms that find the optimal power vector and base station assignment.

deBruijn 그래프의 블럭 분해에 관하여

정치봉 순천향대학교 1993 논문집 Vol.16 No.3

The deBrujin graph ?? is the state diagram for an n-stage binary shift register. It has 2ⁿvertices and ?? edges. In this paper, it is shown that ?? can be bulit by wiring together many isomorphic copies of a fixed block for ??. We discuss the efficiencu of such a building block as the fraction of the edges of ?? which are present in the copies of the building block. We present the problems to find most efficient building block. There results are used to construct a very large Viterbi decoder whose floor plan is the graph ??.

복합재료 샌드위치 판의 고유 진동수에 대한 탄성보의 영향

이봉학,원치문,이정호,김성환 江原大學校 産業技術硏究所 1997 産業技術硏究 Vol.17 No.-

For each construction material used, there is certain theoretical limit in sizes. For tall building construction, the reduction in slab weight is the first step to take in order to break such size limits. In this paper, the feasibility of such objective is proven and given by numerical analysis result. For a typical building slab, both concrete and advanced composite sandwich panels are considered. The concrete slab is treated as special orthotropic plate to obtain more accurate result. Any method may be used to obtain the deflection influence surfaces needed for this vibration analysis. Finite difference method is used for this purpose, in this paper. The influence of the modulus of the foundation on the natural frequency is thoroughly studied.