Problems in Chinese Chorus Art and Countermeasures from a Cultural Perspective

Chen Xiao(Chen Xiao) 한국공공가치학회 2022 공공가치연구 Vol.4 No.1

Gene-metabolite network analysis in different nonalcoholic fatty liver disease phenotypes

Xiao-Lin Liu,Ya-Nan Ming,Jing-Yi Zhang,Xiao-Yu Chen,Min-De Zeng,Yi-Min Mao 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

We sought to identify common key regulators and build a gene-metabolite network in different nonalcoholic fatty liver disease (NAFLD) phenotypes. We used a high-fat diet (HFD), a methionine-choline-deficient diet (MCDD) and streptozocin (STZ) to establish nonalcoholic fatty liver (NAFL), nonalcoholic steatohepatitis (NASH) and NAFL+type 2 diabetes mellitus (T2DM) in rat models, respectively. Transcriptomics and metabolomics analyses were performed in rat livers and serum. A functional network-based regulation model was constructed using Cytoscape with information derived from transcriptomics and metabolomics. The results revealed that 96 genes, 17 liver metabolites and 4 serum metabolites consistently changed in different NAFLD phenotypes (42-fold, Po0.05). Gene-metabolite network analysis identified ccl2 and jun as hubs with the largest connections to other genes, which were mainly involved in tumor necrosis factor, P53, nuclear factor-kappa B, chemokine, peroxisome proliferator activated receptor and Toll-like receptor signaling pathways. The specifically regulated genes and metabolites in different NAFLD phenotypes constructed their own networks, which were mainly involved in the lipid and fatty acid metabolism in HFD models, the inflammatory and immune response in MCDD models, and the AMPK signaling pathway and response to insulin in HFD+STZ models. Our study identified networks showing the general and specific characteristics in different NAFLD phenotypes, complementing the genetic and metabolic features in NAFLD with hepatic and extra-hepatic manifestations.

A Case Study on City Park’s Stormwater Management of Sponge City in China

Chen, Xiao Hui(진효훼),Pei, Fang Ming(배방명),Kim, Shin Won(김신원) 한국디자인문화학회 2018 한국디자인문화학회지 Vol.24 No.4

최근 중국에서 유행하는 경관생태 개념인 스펀지 도시(해면도시)는 점진적으로 중시되고 있다. 본 연구는 스펀지 도시의 이론을 파악해 중국 도시의 선진적인 우수관리 시스템 건설을 위한 기초 개념을 제공하고, 건설 방법을 제시하는 한편 이론적 결과를 바탕으로 하여 실천에 참고가 되는 자료를 제시하는데 연구의 목적을 둔다. 본 논문은 스펀지 도시 이론을 바탕으로 하여 건설된 예시를 연구의 대상으로 정하였다. 연구 방법은 스펀지 도시 이론의 우수관리를 분석하며, 우수관리의 구역적 특성, 조경 디자인의 생태성, 서비스의 다양성 등 3가지 특징을 나타낸다. 그 특징에 의해 지정된 3가지 부족한 면모는 천진 Qiaoyuan(橋園) 습지공원, 첸안 Sanlihe(三里河) 생태행랑과 하얼빈 Qunli(群力) 우수공원을 비교분석하였다. 예시 분석을 통하여 우수관리의 장점과 단점을 발견하여 스펀지 도시의 건설을 위한 지침을 제시하고자 하였다. 연구 결과, 해면체로서의 도시공원은 주민에게 휴식과 오락 장소를 제공함과 동시에 동식물에게 서식지를 제공하기도 하였다. 도시 홍수의 감소 및 수자원 재활용의 측면에서 도시 전체를 여전히 적용하지 않았는데, 지방의 환경은 발전과 개선의 측면에서 적극적으로 발휘되었다. 앞선 연구의 내용으로 보아 이론과 실천 경험의 부족함으로 인해 건설된 해면체로서의 도시공원은 수질 정화 및 지하수 보급의 측면에서 피할 수 없는 결점이 존재한다는 것을 확인하였다. 문화와 역사를 내포한 스펀지 도시를 건설하는 것은 인간의 과거와 미래 문명 발전을 지지하기도 한다. 본 연구는 미래 스펀지 도시의 발전 및 관련 연구에 가치 있는 자료로 활용되기를 기대한다. China has been aware of the popularity of an ecological concept, the Sponge City. This is a study of the theory for the Sponge City which points out an excellent direction of the stormwater management for Chinese cities. Meanwhile, the research of the construction of the Sponge City would provide references for theory and practice studies. This research takes examples of Sponge City as objects for study. The research methodology is infestation and analysis for stormwater management under the Sponge City theory, to summarize the three features of regional characteristics, ecology in landscape design, and diversity of services. By comparing these three features in Tianjin Qiaoyuan Wetland Park, Qian’an Sanlihe Ecological Corridor, and Harbin Qunli Stormwater Park, this paper discovered the advantages and disadvantages of stormwater management, provding inspirational suggestions for the construction of the Sponge City. Comprehensively, the Sponge City can provide residents not only a place to take a rest and entertain but an environment for plants and animals to live as an urban park. Although the problems of city stormwater management and the replenishment of water resources still exist, the Sponge City plays a vital role in the development and improvement of the zonule environment. Due to the lack of theoretical and practical experience, the established Sponge City park has certain defects in water purification and groundwater recharge. As a carrier of culture and history, the construction of the Sponge City also bears the development of human civilization in the past and the future. As a textual material, this research may be used as a reference for the future development of the Sponge City.

SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/β-Catenin Pathway

Chen, Liang-Liang,Gao, Ge-Xin,Shen, Fei-Xia,Chen, Xiong,Gong, Xiao-Hua,Wu, Wen-Jun Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.9

As the most common type of endocrine malignancy, papillary thyroid cancer (PTC) accounts for 85-90% of all thyroid cancers. In this study, we presented the hypothesis that SDC4 gene silencing could effectively attenuate epithelial mesenchymal transition (EMT), and promote cell apoptosis via the $Wnt/{\beta}-catenin$ signaling pathway in human PTC cells. Bioinformatics methods were employed to screen the determined differential expression levels of SDC4 in PTC and adjacent normal samples. PTC tissues and adjacent normal tissues were prepared and their respective levels of SDC4 protein positive expression, in addition to the mRNA and protein levels of SDC4, $Wnt/{\beta}-catenin$ signaling pathway, EMT and apoptosis related genes were all detected accordingly. Flow cytometry was applied in order to detect cell cycle entry and apoptosis. Finally, analyses of PTC migration and invasion abilities were assessed by using a Transwell assay and scratch test. In PTC tissues, activated $Wnt/{\beta}-catenin$ signaling pathway, increased EMT and repressed cell apoptosis were determined. Moreover, the PTC K1 and TPC-1 cell lines exhibiting the highest SDC4 expression were selected for further experiments. In vitro experiments revealed that SDC4 gene silencing could suppress cell migration, invasion and EMT, while acting to promote the apoptosis of PTC cells by inhibiting the activation of the $Wnt/{\beta}-catenin$ signaling pathway. Besides, $si-{\beta}-catenin$ was observed to inhibit the promotion of PTC cell migration and invasion caused by SDC4 overexpression. Our study revealed that SDC4 gene silencing represses EMT, and enhances cell apoptosis by suppressing the activation of the $Wnt/{\beta}-catenin$ signaling pathway in human PTC.

Expression Analysis of miRNAs in Porcine Fetal Skeletal Muscle on Days 65 and 90 of Gestation

Chen, Jian-hai,Wei, Wen-Juan,Xiao, Xiao,Zhu, Meng-Jin,Fan, Bin,Zhao, Shu-Hong Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.7

MiRNAs (microRNAs) are a class of small non-coding RNA molecules of ~21 nucleotides that down- regulate the expression of target genes at post-transcriptional level. In this study, we first accomplished a preliminary scan of miRNA expression using 65 and 90 day fetal pig skeletal muscle samples by microarray hybridization, and 34 miRNAs showed strong positive signals. Five of these miRNAs were selected for further investigation by real-time RT-PCR. The statistical analyses indicated that three miRNAs exhibited significant differential expression (p<0.05) during porcine muscle development from 65 to 90 days of gestation, e.g., miR-24 and miR-424 were down-regulated while miR-133a was up-regulated. Multi-tissue RT-PCR was performed to detect the expression patterns of the five miRNA precursors. The results showed that most of these precursor miRNAs were ubiquitously expressed in different porcine tissues.

Numerical and Experimental Study on Strain Rate Effect of Ordinary Concrete under Low Strain Rate

Xiao Chen,Chong Shi,Yu-Long Zhang,Jun-Xiong Yang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

The strain rate effect of ordinary concrete is researched through the particle discrete element method. A random construction method of three-dimensional aggregate is used, and the numerical sample consistent with the experimental specimen is constructed using particle flow code. Moreover, the simulation method that can properly reflect the strain rate effect with the parallel bond model is proposed. The meso-parameters are calibrated by triaxial compression test. Based on the proposed simulation method and calibrated meso-parameters, the numerical tests of direct tension, uniaxial compression, and cyclic loading under strain rates from 10−5 s−1 to 10−1 s−1 are carried out. The results show that the strain rate effect of concrete can be simulated with particle flow code by assuming that the micromechanical properties of materials vary with the strain rate, and the strength and failure characteristics of numerical samples under different strain rates are described well by the proposed method. In addition, the different mechanical responses of the samples to the strain rate in the compression test and the tensile test are obtained, and the changes of mechanical parameters and damage degree with strain rates in the cyclic loading test are also successfully simulated. This study can provide a feasible numerical method for the follow-up research of dynamic mechanical behavior of concrete and offer theoretical guidance for the stability assessment of concrete engineering.

Adsorption of Nucleotides on ${\beta}$-Cyclodextrin Derivative Grafted Chitosan

Xiao Jian-Bo,Yu Hong-Zhu,Xu Ming,Chen Xiao-Qing The Polymer Society of Korea 2006 Macromolecular Research Vol.14 No.4

A novel ${\beta}$-cyclodextrin derivative (CCD-C) was synthesized with chitosan and carboxymethyl-${\beta}$-cyclodextrin. Its structure was characterized by elemental analysis, infrared spectra analysis, and X-ray diffraction analysis. The adsorption properties for guanosine 5'-monophosphate, cytidine 5'-monophosphate and uridine 5'-monophosphate were studied. Experimental results demonstrated that CCD-C had higher adsorption capability for guanosine 5'-monophosphate, and that the adsorption capacity for guanosine 5'-monophosphate was 74.20mg/g. The adsorption capacity was greatly influenced by pH, time and temperature. The introduction of chitosan enhanced the adsorption ability and adsorption selectivity of ${\beta}$-cyclodextrin for guanosine 5'-monophosphate. This novel derivative of chitosan is expected to have wide applications in the separation, concentration and analysis of nucleotides in biological samples.

Low-Charge Electrotherapy in Geriatric Major Depressive Disorder Patients: A Case Series

Xiao-ming Kong,Xin-hui Xie,Shu-xian Xu,Yang Chen,Chen Wang,Hong Hong,Yan Sun 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.6

To examine the feasibility of low-charge electrotherapy (LCE) in treating geriatric major depressive disorder (MDD) patients. Bi-temporal LCEs (approximately 25 mC) were performed with an electroconvulsive therapy (ECT) instrument three times per week. We used the Hamilton Depression Scale 17 (HAMD-17) and the Hamilton Anxiety Scale (HAMA) to assess the effects of LCE and the Mini- Mental State Examination (MMSE) to evaluate the cognitive function change before and after LCE. Six visits occurred at the baseline, after LCE sessions 3, 6, and 9, after the last session, and at the end of the one-month follow-up period. Four patients were enrolled in the study. Two patients completed all LCE sessions. Two patients withdrew during the trial, one due to the adverse event of uroschesis potentially caused by atropine and the other due to her own will. All four patients completed the follow-up sessions. The HAMD-17 and HAMA scores were reduced significantly at the last LCE session and the end of the follow-up period compared with the scores at the baseline. As measured by the MMSE, cognitive impairment showed no significant changes at the last LCE session and the end of the follow- up period compared with that at the baseline. In this case series, LCE showed potential as an alternative current-based treatment for treating geriatric MDD patients. Further research is needed to assess the efficiency and safety of LCE.

Differences in Physicochemical and Nutritional Properties of Breast and Thigh Meat from Crossbred Chickens, Commercial Broilers, and Spent Hens

Chen, Yulian,Qiao, Yan,Xiao, Yu,Chen, Haochun,Zhao, Liang,Huang, Ming,Zhou, Guanghong Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.6

The objective of this study was to compare the physicochemical and nutritional properties of breast and thigh meat from commercial Chinese crossbred chickens (817 Crossbred chicken, 817C), imported commercial broilers (Arbor Acres broiler, AAB), and commercial spent hens (Hyline Brown, HLB). The crossbred chickens, commercial broilers and spent hens were slaughtered at their typical market ages of 45 d, 40 d, and 560 d, respectively. The results revealed that several different characteristic features for the three breeds. The meat of the 817C was darker than that of the other two genotypes. The 817C were also characterized by higher protein, lower intramuscular fat, and better texture attributes (cooking loss, pressing loss and Warner-Bratzler shear force [WBSF]) compared with AAB and HLB. The meat of the spent hens (i.e. HLB) was higher in WBSF and total collagen content than meat of the crossbred chickens and imported broilers. Furthermore, correlation analysis and principal component analysis revealed that there was a clear relationship among physicochemical properties of chicken meats. With regard to nutritional properties, it was found that 817C and HLB exhibited higher contents of essential amino acids and essential/non-essential amino acid ratios. In addition, 817C were noted to have highest content of microelements whereas AAB have highest content of potassium. Besides, 817C birds had particularly higher proportions of desirable fatty acids, essential fatty acids, polyunsaturated/saturated and (18:0+18:1)/16:0 ratios. The present study also revealed that there were significant differences on breast meat and thigh meat for the physicochemical and nutritional properties, regardless of chicken breeds. In conclusion, meat of crossbred chickens has some unique features and exhibited more advantages over commercial broilers and spent hens. Therefore, the current investigation would provide valuable information for the chicken meat product processing, and influence the consumption of different chicken meat.

Algebraic Dynamics Investigation for a Trigonal Heterotrinuclear Spin Cluster in a Rotating Magnetic Field

Chen Xiao-Xu,Xue Jun 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.2

We investigate the heterotrinuclear trigonal spin cluster (each particle with S = 1/2) driven by a time-dependent magnetic field. Using an algebraic dynamics method, we solve the time-dependent Schrodinger equation of the spin-cluster system and give an alternative expression for the non-adiabatic geometric phase or the Berry phase when the magnetic field rotates around the $Z$ axis. Based on the exact analytical solutions, we show how the Berry phase depends on the external magnetic field parameters $\omega$ (the angular velocity of the rotating magnetic field) and $\theta$ (the angle between the magnetic field and the $Z$ axis), and we discuss the changing diagram of the Berry phase of the system in the ground state.