Analysis of Indoleamine 2-3 Dioxygenase (IDO) and EGFR Co-expression in Breast Cancer Tissue by Immunohistochemistry

Bi, Wei-Wei,Zhang, Wei-Hua,Yin, Gui-Hua,Luo, Hong,Wang, Shou-Qin,Wang, Hongran,Li, Chao,Yan, Wei-Qun,Nie, De-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: To determine the amount of co-expression of IDO and EGFR in breast cancer patients. Materials and Methods:In order to obtain the distribution of co-expression of IDO and EGFR in breast cancer, we tested 110 breast cancer paraffin tissue blocks with immunohistochemical methods. Then we investigated the relationship between the diagnostic and pathologic characteristics (tumor size, lymph node status, histologic grade, the gene expression of ER, PR, HER2, p53, Ki67 and PCNA) with the situation of co-expression of IDO and EGFR by reviewing the medical records of 32 breast cancer patients. Results: Among 110 breast cancers, 32 cases demonstrated IDO and EGFR co-expression (29.1%), IDO and EGFR synchronous co-expression being found in 19.1% and asynchronous in 10.0%. Conclusions: IDO and EGFR were co-expressed in breast cancer, including synchronous and asynchronous co-expression. The results suggest that considering IDO and EGFR as two indicators for breast cancer treatment or prognosis analysis provides a potential option of individual treatment for the portion of breast cancer patients with co-expression of IDO and EGFR.

Experimental Study on Sustained-release 5-Fluorouracil Implantation in Canine Peritoneum and Para-aortic Abdominalis

Wei, Guo,Nie, Ming-Ming,Shen, Xiao-Jun,Xue, Xu-Chao,Ma, Li-Ye,Du, Cheng-Hui,Wang, Shi-Liang,Bi, Jian-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Objective: To observe local and systemic toxicity after sustained-release 5-fluorouracil (5-Fu) implantation in canine peritoneum and para-aortic abdominalis and the changes of drug concentration in the local implanted tissue with time. Methods: 300 mg sustained-release 5-Fu was implanted into canine peritoneum and para-aorta abdominalis. Samples were taken 3, 5, 7 and 10 days after implantation for assessment of changes and systemic reactions. High performance liquid chromatography was applied to detect the drug concentrations of peritoneal tissue at different distances from the implanted site, lymphatic tissue of para-aortic abdominalis, peripheral blood and portal venous blood. Results: 10 days after implantation, the drug concentrations in the peritoneum, lymphatic tissue and portal vein remained relatively high within 5 cm of the implanted site. There appeared inflammatory reaction in the local implanted tissue, but no visible pathological changes such as cell degeneration and necrosis, and systemic reaction like anorexia, nausea, vomiting and fever. Conclusions: Sustained-release 5-Fu implantation in canine peritoneum and para-aortic abdominalis can maintain a relatively high tumour-inhibiting concentration for a longer time in the local implanted area and portal vein, and has mild local and systemic reactions. Besides, it is safe and effective to prevent or treat recurrence of gastrointestinal tumours and liver metastasis.

위안화 환율 변화의 중국 수출입에 대한 영향 분석

필위녕(Bi, Wei Ning),임방방(Ren, Fang Fang),남수중(Nam, Soo Joong) 영남대학교 중국연구센터 2014 중국과 중국학 Vol.22 No.-

본 논문은 요한슨 공적분 검정법과 중력모형을 이용하여 위안화 환율변화가 중국 용도별 수출입에 미친 영향을 실증적으로 분석하였다. 1998년부터 2012년까지 중국과 20개 교역대상국의 연도별 패널데이터를 고정효과, 확률효과와 최소자승법(OLS)으로 나누어 분석하였다. 본 논문의 주요 결과는 다음과 같은 의미를 갖는다. 첫째, 환율의 변화와 수출입 사이에는 장기적 균형관계가 존재하는 것으로 나타났다. 환율의 수출 및 수입 수요 탄력성의 절대값 합이 3.09에 달해 1보다 크다는 것을 알 수 있었다. 즉 마샬-러너 조건이 충족되었다. 본 연구는 위안화의 명목환율 변화가 장기적으로 중국 수출입에 영향을 미칠 수 있음을 규명하였다. 위안화 환율변화가 중국의 수출입에 미치는 영향을 추정한 결과에 따르면 수출보다는 수입이 환율변동에 대해 더 민감하게 반응하는 것을 알 수 있었다. 둘째, 중국과 외국의 GDP 변화가 수출입에 미친 영향을 보면, 중국의 수출에 대한 외국의 GDP 영향, 즉 외국의 수입 수요는 모두 통계적으로 1%에서 유의미하였으나, 중간재와 소비재보다 자본재의 수출에 대한 영향이 가장 컸던 것으로 파악되었다. 또한 수입의 경우, 중국 국내수입 수요, 즉 GDP 증가는 자본재, 중간재, 소비재 모두 회귀계수 값이 높지 않은 반면, 중간재와 소비재의 경우 통계적으로 1%에서 유의하였다. 셋째, 실증분석 결과는 실질실효 환율이 절상된다면 자본재, 중간재, 소비재 수출이 모두 증가하는 것으로 나타났다. 모두 통계적으로도 유의하였다. 반면 수입의 경우, 환율 변화의 영향이 제한적인 것으로 추정되었다. 특히 자본재의 수입이 통계적으로 유의미한 값을 제시하지 못하였다. This paper focuses on the long-term and short-term impact of RMB exchange rate on China’s export and import trade to 20 countries from 1980 to 2012. Through the Johansen cointegration test and gravity model, this paper concludes that the export is a positive to the change of exchange rate in the long-term, but the import is negative. The exchange rate elasticity of the import is larger than the export. As a result, Marshall-Lerner condition is valid. It is 3.09, more high than 1. Therefore, the GDP of China and foreign countries are the important factors and possesses a significantly positive impact on China’s exports and imports. The empirical model reveals that the effects of RMB real effective exchange rate are a significantly positive on capital goods, intermediate goods and consumption goods exports. But China’s imports have not any significant results statistically, particularly on capital goods imports. From the empirical result, it is proved that exchange rate flexibility has the obvious influence on China’s export and import trade in the short and long term.

Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

Jiansheng Wei,Liangyan Liu,Xiaolong Yuan,Dong Wang,Xinyue Wang,Wei Bi,Yan Yang,Yi Wang 한국균학회 2023 Mycobiology Vol.51 No.5

Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang. To analyzehispidin biosynthesis in S. sanghuang, the transcriptomes of hispidin-producing and non-producingS. sanghuang were determined by Illumina sequencing. Five PKSs were identifiedusing genome annotation. Comparative analysis with the reference transcriptome showedthat two PKSs (ShPKS3 and ShPKS4) had low expression levels in four types of media. Thegene expression pattern of only ShPKS1 was consistent with the yield variation of hispidin. The combined analyses of gene expression with qPCR and hispidin detection by liquid chromatography-mass spectrometry coupled with ion-trap and time-of-flight technologies(LCMS-IT-TOF) showed that ShPKS1 was involved in hispidin biosynthesis in S. sanghuang. ShPKS1 is a partially reducing PKS gene with extra AMP and ACP domains before the KSdomain. The domain architecture of ShPKS1 was AMP-ACP-KS-AT-DH-KR-ACP-ACP. Phylogenetic analysis shows that ShPKS1 and other PKS genes from Hymenochaetaceaeform a unique monophyletic clade closely related to the clade containing Agaricales hispidinsynthase. Taken together, our data indicate that ShPKS1 is a novel PKS of S. sanghuanginvolved in hispidin biosynthesis.

Naringin and Naringenin Relax Rat Tracheal Smooth by Regulating BKCa Activation

Rui Shi,Jia-Wen Xu,Zi-Ting Xiao,Ruo-Fei Chen,Yi-Lin Zhang,Jia-Bi Lin,Ke-Ling Cheng,Gu-Yi Wei,Pei-Bo Li,Wen-Liang Zhou,Wei-Wei Su 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.9

Naringin and its aglycone, naringenin, occur naturally in our regular diet and traditional Chinese medicines. This study aimed to detect an effective therapeutic approach for cough variant asthma (CVA) through evaluating the relaxant effect of these two bioactive herbal monomers as antitussive and antiasthmatic on rat tracheal smooth muscle. The relaxant effect was determined by measuring muscular tension with a mechanical recording system in rat tracheal rings. Cytosolic Ca2+ concentration was measured using a confocal imaging system in primary cultured tracheal smooth muscle cells. In rat tracheal rings, addition of both naringin and naringenin could concentration dependently relax carbachol (CCh)-evoked tonic contraction. This epithelium-independent relaxation could be suppressed by BaCl2, tetraethylammonium, and iberiotoxin (IbTX), but not by glibenclamide. After stimulating primary cultured tracheal smooth muscle cells by CCh or high KCl, the intracellular Ca2+ increase could be inhibited by both naringin and naringenin, respectively. This reaction was also suppressed by IbTX. These results demonstrate that both naringin and naringenin can relax tracheal smooth muscle through opening big conductance Ca2+-activated K+ channel, which mediates plasma membrane hyperpolarization and reduces Ca2+ influx. Our data indicate a potentially effective therapeutic approach of naringin and naringenin for CVA.

Crystal Structure and Tautomerism Study of the Mono-protonated Metformin Salt

Wei, Xiaodan,Fan, Yuhua,Bi, Caifeng,Yan, Xingchen,Zhang, Xia,Li, Xin Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.12

A novel crystal, the mono-protonated metformin acetate (1), was obtained and characterized by elemental analysis, IR spectroscopy and X-ray crystallography. It was found that one of the imino group in the metformin cation was protonated along with the proton transfer from the secondary amino group to the other imino group. Its crystal structure was then compared with the previously reported diprotonated metformin oxalate (2). The difference between them is that the mono-protonated metformin cations can be linked by hydrogen bonding to form dimers while the diprotonated metformin cations cannot. Both of them are stabilized by intermolecular hydrogen bonds to assemble a 3-D supermolecular structure. The four potential tautomer of the mono-protonated metformin cation (tautomers 1a, 1b, 1c and 1d) were optimized and their single point energies were calculated by Density Functional Theory (DFT) B3LYP method based on the Polarized Continuum Model (PCM) in water, which shows that the most likely existed tautomer in human cells is the same in the crystal structure. Based on the optimized structure, their Wiberg bond orders, Natural Population Analysis (NPA) atomic charges, molecular electrostatic potential (MEP) maps were calculated to analyze their electronic structures, which were then compared with the corresponding values of the diprotonated metformin cation (cation 2) and the neutral metformin (compound 3). Finally, the possible tautomeric mechanism of the mono-protonated metformin cation was discussed based on the observed phenomena.

Performance and Applicability Analysis of Recognizing Microseismic Waveforms Using Neural Networks in Tunnel Engineering

Wei Zhang,Xin Bi,Lei Hu,Pengxiang Li,Zhibin Yao 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

The sudden and harmful nature of rockbursts in tunnels necessitates an accurate and applicable method for automatically recognizing rock fracture signals during rockburst microseismic (MS) monitoring. In this paper, the performance and applicability of recognizing MS waveforms using an artificial neural network (ANN) and a deep neural network (DNN) were studied in tunnels excavated by different methods. The results show that ANN performs very well in recognizing rock fracturing waveforms with a signal-to-noise ratio (SNR) ≥ 3 but has a low accuracy for those with an SNR < 3. The DNN also performs well for waveforms with SNR ≥3, and has a relatively high accuracy for waveforms with SNR < 3. The ANN model can be used in tunnels excavated by drilling and blasting (D&B) since there are fewer “small” rock fracturing events. The DNN model is applicable in tunnels excavated by the tunnel boring machine (TBM), recognizing more “small” events. In addition, the ANN model is a better choice, with fewer training samples at the initial stage of monitoring working. With continuous monitoring, the DNN model can be used to ensure and improve the accuracy. These results lay a foundation for automatic rockburst MS monitoring techniques in tunnel engineering.

Characterization and evolutionary analysis of Brassica species-diverged sequences containing simple repeat units

Lijuan Wei,Meili Xiao,Annaliese S. Mason,Bi Ma,Kun Lu,Jia Na Li,Link Katrin,Donghui Fu 한국유전학회 2013 Genes & Genomics Vol.35 No.2

Brassica species, B. napus (canola), B. rapa and B. oleracea, are important sources of nutritionally valuable vegetable oil and protein-rich meal for animals and humans. Sequencing of the model plant Arabidopsis thaliana, has opened the way for investigations into the complex structure of the Brassica genomes, offering important insight into their evolution and composition. We use this sequence information for the characterization and functional analysis of SSR sequences that have diverged between the Brassica species. A total of 56 species-diverged sequences containing simple repeat units (SDS-SSR) of B. napus and its diploid progenitor species B. rapa and B. oleracea were isolated and characterized. Of these, 40 sequences showed homology with other Brassica sequences. Using the SSR Locator software, only 23 sequences were found to have SSRs,possibly due to the loss of SSR units in the process of species divergence. Sequence alignments with A. thaliana revealed that these species-diverged SSR sequences were responsible for Brassica divergence for differences between Brassica species in several genomic regions. Six active genes related to transferase, protein, transcription factor and retroelements were found in the SDS-SSRs. These results will further improve our understanding of the characteristics of species-diverged SSR fragments and their contribution to genome differentiation.

Proliferated Leydig Cells for Engineered Testis-like Tissue Regeneration with Testosterone-Secreting Ability

Hongda Bi,Xiaoyun Wang,Wei Liu,Yilin Cao,Guangdong Zhou,Xin Xing 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.5

Tissue engineering approach provides a hopeful strategy for reconstructing testis testosterone-secreting functions. However, limited source and low proliferative activity in vitro of Leydig cells (LCs, the main testosteroneproducing cells) makes testis-like tissue regeneration difficult to be achieved. This study explored the feasibility of in vitro expanding LCs and their potential application in testis-like tissue regeneration. LC lineage cells were isolated from Sprague-Dawley (SD) rats by differential adhesion method and cell composition was identified by expressions of 3β-HSD, LHR, LIFR, and c-kit. A modified expansion medium (EM) system was used to test the feasibility of in vitro expanding LC lineage. The results showed that the attached cells reached a high purification of LC lineage (>90%, indicated by positive expression of 3β-HSD) and that EM significantly enhanced proliferation of LC lineage compared to regular medium, which was testified to be related to the presence of stem LCs that was implied by positive expressions of LIFR and c-kit as well as the transition of 3β-HSD expression from negative to positive in partial cells. Importantly, the proliferated LCs showed relatively sustained testosterone-secreting ability in vitro and these cells combined with biodegradable scaffolds successfully regenerated testis-like tissue with sustained testosteronesecreting function in vivo, which was supported by the enhanced serum testosterone level in castrated rats. All these results indicated that the differential adhesion method could efficiently isolate and purify LC lineage and that EM system could efficiently promote proliferation and functional maintenance of LC lineage, providing a good cell source for testes-like tissue regeneration. Tissue engineering approach provides a hopeful strategy for reconstructing testis testosterone-secreting functions. However, limited source and low proliferative activity in vitro of Leydig cells (LCs, the main testosteroneproducing cells) makes testis-like tissue regeneration difficult to be achieved. This study explored the feasibility of in vitro expanding LCs and their potential application in testis-like tissue regeneration. LC lineage cells were isolated from Sprague-Dawley (SD) rats by differential adhesion method and cell composition was identified by expressions of 3β-HSD, LHR, LIFR, and c-kit. A modified expansion medium (EM) system was used to test the feasibility of in vitro expanding LC lineage. The results showed that the attached cells reached a high purification of LC lineage (>90%, indicated by positive expression of 3β-HSD) and that EM significantly enhanced proliferation of LC lineage compared to regular medium, which was testified to be related to the presence of stem LCs that was implied by positive expressions of LIFR and c-kit as well as the transition of 3β-HSD expression from negative to positive in partial cells. Importantly, the proliferated LCs showed relatively sustained testosterone-secreting ability in vitro and thesecells combined with biodegradable scaffolds successfully regenerated testis-like tissue with sustained testosteronesecreting function in vivo, which was supported by the enhanced serum testosterone level in castrated rats. All these results indicated that the differential adhesion method could efficiently isolate and purify LC lineage and that EM system could efficiently promote proliferation and functional maintenance of LC lineage, providing a good cell source for testes-like tissue regeneration.

Universal Scale Tipping towards Balance-Applying the MFN Clauses in China-related Investment Arbitration: A New Haven School Reading

Yuanchao Bi,Wei Shen 이준국제법연구원 2020 China and WTO Review Vol.6 No.2