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        Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

        Jiansheng Wei,Liangyan Liu,Xiaolong Yuan,Dong Wang,Xinyue Wang,Wei Bi,Yan Yang,Yi Wang 한국균학회 2023 Mycobiology Vol.51 No.5

        Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang. To analyzehispidin biosynthesis in S. sanghuang, the transcriptomes of hispidin-producing and non-producingS. sanghuang were determined by Illumina sequencing. Five PKSs were identifiedusing genome annotation. Comparative analysis with the reference transcriptome showedthat two PKSs (ShPKS3 and ShPKS4) had low expression levels in four types of media. Thegene expression pattern of only ShPKS1 was consistent with the yield variation of hispidin. The combined analyses of gene expression with qPCR and hispidin detection by liquid chromatography-mass spectrometry coupled with ion-trap and time-of-flight technologies(LCMS-IT-TOF) showed that ShPKS1 was involved in hispidin biosynthesis in S. sanghuang. ShPKS1 is a partially reducing PKS gene with extra AMP and ACP domains before the KSdomain. The domain architecture of ShPKS1 was AMP-ACP-KS-AT-DH-KR-ACP-ACP. Phylogenetic analysis shows that ShPKS1 and other PKS genes from Hymenochaetaceaeform a unique monophyletic clade closely related to the clade containing Agaricales hispidinsynthase. Taken together, our data indicate that ShPKS1 is a novel PKS of S. sanghuanginvolved in hispidin biosynthesis.

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        Corrosion Cracking Process of Reinforced Concrete under the Coupled Effects of Chloride and Fatigue Loading

        Jiansheng Shen,Junzhe Liu,Yidong Xu,Kanghao Jia,Fangyu Wu,Wei Chen,Dayong Zhu 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.9

        To study the corrosion cracking process of reinforced concrete under the combined effects of chloride and fatigue loading, the constan-current and dry-wet cycle accelerated corrosion method was used to corrosion the specimens under different stress levels for different time. The quality loss of reinforcement, the composition of corrosion products and the cracking of concrete are analyzed from the macro, micro and micro scales, and to obtain the spatial distribution as well as microscopic characteristics of corrosion products of the reinforcement bar under coupling conditions. Additionally, a model of steel rust cracking under the coupled action of chloride and fatigue loading is established. The results show that: under the same corrosion time, the concrete cracking and steel corrosion degree become more serious with the increase of stress level. The greater the stress level, the earlier corrosion occurs and the more corrosion products are, Moreover, due to the fatigue load, the concrete on the upper side of the steel bar has rust expansion cracks earlier than the lower side.

      • KCI등재

        Related-Key Differential Attacks on CHESS-64

        ( Wei Luo ),( Jiansheng Guo ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.9

        With limited computing and storage resources, many network applications of encryption algorithms require low power devices and fast computing components. CHESS-64 is designed by employing simple key scheduling and Data-Dependent operations (DDO) as main cryptographic components. Hardware performance for Field Programmable Gate Arrays (FPGA) and for Application Specific Integrated Circuits (ASIC) proves that CHESS-64 is a very flexible and powerful new cipher. In this paper, the security of CHESS-64 block cipher under related-key differential cryptanalysis is studied. Based on the differential properties of DDOs, we construct two types of related-key differential characteristics with one-bit difference in the master key. To recover 74 bits key, two key recovery algorithms are proposed based on the two types of related-key differential characteristics, and the corresponding data complexity is about 2<sup>42.9</sup> chosen-plaintexts, computing complexity is about 2<sup>42.9</sup> CHESS-64 encryptions, storage complexity is about 2<sup>26.6</sup> bits of storage resources. To break the cipher, an exhaustive attack is implemented to recover the rest 54 bits key. These works demonstrate an effective and general way to attack DDO-based ciphers.

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        Sustainable production of acetaldehyde from lactic acid over the carbon catalysts

        Cong-Ming Tang,Jiansheng Peng,Xin-Li Li,Zhanjie Zhai,Hejun Gao,Wei Bai,Ning Jiang,Yunwen Liao 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.1

        The synthesis of acetaldehyde from lactic acid over the carbon material catalysts was investigated. The carbon materials were characterized by scanning electron microscopy for morphologic features, by X-ray diffraction for crystal phases, by Fourier transform infrared spectroscopy for functional group structures, by N2 sorption for specific surface area and by ammonia temperature-programed desorption for acidity, respectively. Among the tested carbon catalysts, mesoporous carbon displayed the most excellent catalytic performance. By acidity analysis, the medium acidity is a crucial factor for catalytic performance: more medium acidity favored the formation of acetaldehyde from lactic acid. To verify, we compared the catalytic performance of fresh activated carbon with that of the activated carbon treated by nitric acid. Similarly, the modified activated carbon also displayed better activity due to a drastic increase of medium acidity amount. However, in contrast to fresh carbon nanotube, the treated sample displayed worse activity due to decrease of medium acidity amount. The effect of reaction temperature and time on stream on the catalytic performance was also investigated. Under the optimal reaction conditions, 100% lactic acid conversion and 91.6% acetaldehyde selectivity were achieved over the mesoporous carbon catalyst.

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