국내 젖소에서 Theileria buffeli 주요 표면 단백질 유전자의 다양성 분석

유도현,이영화,채준석,박진호,Yu, Do-Hyeon,Li, Ying-Hua,Chae, Joon-Seok,Park, Jin-Ho 대한수의학회 2011 大韓獸醫學會誌 Vol.51 No.2

Theileria (T.) buffeli (formerly T. sergenti/T. orientalis) is the major hemo-protozoan distributed in the Far East Asian countries such as Korea, China and Japan. It is responsible for the clinical symptoms of anorexia, ateliosis, anemia, fever and icterus. It also causes abortion and sudden death under severe cases, resulting in economic losses for many livestock farms. The objective of this study was to analyze the genetic diversity of the major surface protein (Msp) gene in T. buffeli in Holstein in Korea, and we characterized the association of the diversification of the Msp gene and its relationship with the pathogenicity of Theileria. For this, complete blood counts and Theileria PCR sequence analysis were performed from 57 Holstein in Jeju Island. A total of 26 PCR positive Holstein (16 anemic and 10 non-anemic) were then randomly selected based on 18s rRNA sequence typing of the Theileria Msp gene. The DNA sequence of the T. buffeli Msp gene in Holstein showed 99.0%, 99.2%, 99.9%, 99.5%, 98.7%, 98.4% and 98.4% homology with T. sergenti, Theileria spp., T. sergenti, Theileria spp., Theileria spp., Theileria spp. and Theileria spp., respectively. The result showed a genetic variation of 57.7% (type I), 3.8% (type II), 15.4% (type III), 7.7% (type IV), 13.5% (type V) and 1.9% (type VI). Type I is the most frequent type in both anemic and non-anemic Holstein while type II was found in only non-anemic Holstein. This results of our study help confirm the diversity of Msp gene types and demonstrate that the gene type distribution of Msp genes varies among Theileria-infected Holstein in Jeju Island.

FakeImage 판별기를 이용한 합성 영역 검출

박도현(Do-Hyeon Park),유도현(Do-Hyeon Yu),김덕원(Duk-Woen Kim),정승도(Seungdo Jeong) 대한전기학회 2023 대한전기학회 학술대회 논문집 Vol.2023 No.7

우리나라 중남부지역 젖소목장에서 이등유 발생 조사

정지영 ( Ji Young Jung ),유도현 ( Do Hyeon Yu ),신성식 ( Sung Shik Shin ),손창호 ( Chang Ho Son ),오기석 ( Ki Seok Oh ),허태영 ( Tai Young Hur ),정영훈 ( Young Hun Jung ),최창용 ( Chang Yong Choi ),서국현 ( Guk Hyun Suh ) 한국동물위생학회 2015 韓國家畜衛生學會誌 Vol.38 No.3

The incidence of second-grade milk production in 9 dairy farms of South Korea was investigated from May 2011 to March 2012, and the serum composition of cows producing first- and second-grade milk in 14 farms including the 9 farms was analyzed. The incidence rate of second-grade milk production of 402 cows in nine dairy farms located in the central and southwestern regions of Korea was 15.4% with the highest rate being 34.4%. Seasonal morbidity was higher during late winter (February) and early summer (June) with the highest rate observed in February (32.6%) followed by November (33.3%). Second-grade milk was most frequently found within one month postpartum (34.1%) while only 3.5% was found during the first 60∼90 days of lactating period (n=785, 5 herds). The morbidity increased thereafter (P<0.05) with the highest observed between 270∼300 days of lactation (36.1%). The acidity was not significantly different between second-grade (0.159±0.026%) and first-grade milk (0.158±0.027%). Blood serum analysis of 371 cows in the 14 dairy farms indicated that aspartate aminotransferase (AST) level was significantly higher (P<0.001) in cows producing second-grade milk while albumin was significantly lower (P<0.001) than cows producing first-grade milk. Total protein and triglyceride was also significantly low along with glucose, non-esterified fatty acid and blood urea nitrogen in cows producing second-grade milk. Statistical analysis including sensitivity, specificity and positive/negative prediction values showed that lactating cows with high AST, low albumin, total protein and triglyceride levels in the serum tended to produce second-grade milk. It was concluded that serological parameters, especially live functional and metabolic-related serum compositions (AST, albumin, total protein and triglyceride), were significantly influenced in cows producing second-grade milk.

법제유황의 실용적 제조에 따른 물리 화학적 분석 및 독성, 항균 작용에 관한 연구

인동철 ( Dong Chul In ),유도현 ( Do Hyeon Yu ),박철 ( Chul Park ),박진호 ( Jin Ho Park ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.3

Despite of a long history of the sulfur on the disease healing effect, there were limited ways of applying sulfur to animal and human. We have developed the detoxified sulfur (non toxic sulfur) method to make it practical and mass production possible through laboring for many years. This study practiced scanning electron microscope (SEM), Energy dispersive X-ray spectrometer (EDS) and secondary ion mass spectrometry (SIMS) analysis to investigate the physicochemical aspect of detoxified sulfur. We also performed the oral toxicity experiment to mice, and anti-bacterial test of the detoxified sulfur. Based on the SEM, EDS and SIMS results, the united particles in the mass form with the similar component intensity with the raw sulfur were observed, and hydrogen sulfide ion (HS-) component which is regarded as a toxic matter, was decreased after detoxification. Indeed, toxicity test on the mice (10 males, 10 females) showed no clinical, histopathological changes with the 5 times amount (2,500 mg/kg) of the actual doses. However, the male-mice showed decreased in body weight by 23.6%, 24.3% in the 7th, 14th day, respectively, after detoxified sulfur. Moreover, the female-mice administered the detoxified sulfur showed decreased in body weight by 28.7% (P<0.05) than that in the control group on the 14th day. The result of antibacterial test on the detoxified sulfur showed antibacterial effect (27%) to inhibit the growth of Staphylococcus aureus. It is shown that detoxified sulfur can be used as feed additive and has an affect on the farm perfomance.

관악산에서 참진드기 조사 및 중증열성혈소판감소증후군 바이러스 검출

채정병,김태희,정지호,박윤지,박진호,최경성,유도현,박배근,채준석,Chae, Jeong-Byoung,Kim, Tae-Hee,Jung, Jee-Ho,Park, Yoon-Ji,Park, Jin-Ho,Choi, Kyoung-Seong,Yu, Do-Hyeon,Park, Bae-Keun,Chae, Joon-Seok 대한수의학회 2017 大韓獸醫學會誌 Vol.57 No.3

This study was performed to investigate the distribution of ticks and the rate of infection with severe fever with thrombocytopenia syndrome (SFTS) virus in ticks collected at Mt. Gwanak and the Seoul National University campus, Korea. Ticks (n = 273) were collected from May to October and included 76 Haemaphysalis longicornis (4 adult females, 72 nymphs), 49 Haemaphysalis flava (9 adult females, 3 adult males, 37 nymphs), and 148 Haemaphysalis spp. larvae. SFTS virus detection was performed by using one-step RT PCR and nested PCR. The SFTS virus was detected in 7 samples (1 Haemaphysalis longicornis nymph, 3 Haemaphysalis flava nymphs, and 3 Haemaphysalis spp. larva). The overall minimum field infection rate was 2.6%, whereas the minimum field infection rates of adult, nymphal, and larval ticks were 0%, 3.2%, and 2.0%, respectively. For a more accurate indication of the prevalence of SFTS virus in Korea, further in-depth investigations of tick species and SFTS virus occurrence over a larger area and longer period are needed.

Thioacetamide로 유발된 간 손상에 대한 Taraxacum officinale (Dandelion) 추출물의 효과

조인영 ( In Young Cho ),마세령 ( Se Ryung Ma ),문선진 ( Sun Jin Moon ),유도현 ( Do Hyeon Yu ),신성식 ( Sung Shik Shin ),손창호 ( Chang Ho Son ),오기석 ( Ki Seok Oh ),허태영 ( Tai Young Hur ),정영훈 ( Young Hun Jung ),최창용 ( Chan 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4

The genus Taraxacum, known by the common name Dandelion, is a medicinal herb in the family Asteraceae. It has been traditionally used as a folk medicine for the treatment or prevention of various diseases due to its anti-inflammatory and anti-oxidative properties. In this study, we attempted to evaluate protective effects of Dandelion related with anti-oxidative activity to Thioacetamide (TAA)-induced liver damage. 36 rats were randomly assigned to six experimental groups:Control, Dandelion water extract (DWE), TAA, TAA&DWE 300, TAA&DWE 600, TAA&DWE 1,200 groups. Rats in DWE and TAA&DWE groups were pretreated with DWE (300, 600 or 1,200 mg/kg BW) by gavage for 7 days. All rats were treated intraperitoneally with TAA (200 mg/kg BW) or normal saline at 12 hours after last oral administration and sacrificed at 12 hours after last treatment. Levels of WBC and Neutrophil count were significantly decresed in TAA&DWE 1,200 group compared with that in TAA group (P <0.05). In TAA&DWE 600 and TAA&DWE 1,200 groups, serum AST, ALT, GGT levels were lower than TAA group (P<0.05). The serum TG level was significantly elevated in TAA&DWE groups compared with those in TAA group. Liver tissues from TAA group showed extensive histopathological changes, characterized by moderate or severe hepatocytes degeneration, inflammatory cell infiltration, and congestion. In the TAA&DWE group, The severity of histopathological lesions were decreased compared to those in the TAA group. The MDA concentration was significantly decreased and GSH content was significantly increased in the TAA&DWE 1,200 group compared to those in the TAA group. GR, CAT and GST activities in the TAA&DWE 1,200 group were significantly increased compared to those in the TAA group.

우리나라 유산양 알코올 양성유의 특징에 관한 연구

김혜라 ( Hye Ra Kim ),정지영 ( Ji Young Jung ),조인영 ( In Young Cho ),유도현 ( Do Hyeon Yu ),신성식 ( Sung Shik Shin ),손창호 ( Chang Ho Son ),오기석 ( Ki Seok Ok ),허태영 ( Tai Young Hur ),정영훈 ( Young Hun Jung ),최창용 ( Chan 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4

The objective of the present study was to determine the minimum alcohol (ethanol) concentration that gives rise to the coagulation of goat milk for the alcohol precipitation test, and to evaluate the physical parameters of goat milk which include alcohol and heat stability. A total of 1,295 udder-half milk samples from 648 lactating dairy goats were collected from seven farms in Jeonnam province, Republic of Korea, to determine the alcohol and heat stability. The majority (99.6%) of the samples were coagulated when 70% ethanol was added to the milk, while only 11.0% of the samples were precipitated by the addition of an equal volume of 45% ethanol. With the concentration of 65%, 60%, 55% and 50% aqueous ethanol, 99.2%, 96.8%, 81.0% and 52.8% of the milk samples were coagulated, respectively. Of 1,295 dairy goat milk samples tested for heat stability, 127 (9.8%) were coagulated by boiling. Among the 143 alcohol test-positive udder-half milk samples, 52 (4.0%) were unstable by heat test, while 1,032 (79.7%) of the 1,152 alcohol test-negative milk samples were stable by heat test. According to the results of boiling test, sensitivity and specificity of 45% alcohol precipitation test were 0.3023 (95% CI: 0.2346∼0.3772) and 0.9190 (95% CI: 0.9017∼0.9344), respectively. The contents of protein and the specific gravity were higher in the milk samples of 45% alcohol test-positive than in those of 45% alcohol test-negative. However, lower levels of lactose and milk urea nitrogen were observed in the milk samples of 45% alcohol test-positive compared to the alcohol test-negative milk samples. The lowest pH values (6.73±0.20) were shown in the 45% alcohol test-negative and heat-unstable milk samples, while the lowest values of somatic cell counts and bacterial counts were shown in the 45% alcohol test-negative and heat-stable milk samples. Results of this study suggest that the alcohol precipitation for dairy goat milk may have to be tested with ethanol concentration less than 45% for the determination of freshness and heat-stability.

전남지방 산양유의 성분 및 체세포수의 계절적 변화

김혜라 ( Hye Ra Kim ),정지영 ( Ji Young Jung ),조인영 ( In Young Cho ),유도현 ( Do Hyeon Yu ),신성식 ( Sung Shik Shin ),손창호 ( Chang Ho Son ),오기석 ( Ki Seok Ok ),허태영 ( Tai Young Hur ),정영훈 ( Young Hun Jung ),최창용 ( Chan 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4

Consistent information on the chemical composition and its seasonal variation of goat udder half milk is limited in Korea. The objective of this study was to analyze the seasonal variation of the chemical composition of goat milk to take establish various parameters into consideration on the pricing of the goat milk. Variations in chemical composition, somatic cell count (SCC) and bacterial count of 1,038 udder half milk samples from 650 heads raised in 7 farms of Jeonnam province were determined by season. Fat, protein, lactose, non-fat solids, milk urea nitrogen (MUN), pH, SCC and bacterial counts were also analyzed. The average composition of the milk was: fat 3.80±1.36%, protein 3.23±0.80%, lactose 4.39±0.54%, total solids 12.18±1.80%, non-fat solids 8.38±0.80%, and milk urea nitrogen 28.44±5.00 mg/dL. The average pH was 6.81±0.24. The average of SCC and bacterial counts were 2.54±4.60×106 cells/mL and 1.25±3.76×105 CFU/mL, respectively. Chemical composition, pH, SCC and bacterial counts of dairy goat milk varied widely during the lactation period and by season. The fat concentration was the lowest in spring (3.39±1.53%) and the highest in autumn and winter (3.98±1.30% and 3.98±1.48%). Protein concentration was the lowest during summer (2.92±0.48%) and the highest in winter (3.81±0.95%). Lactose concentration was the lowest in autumn (4.24±0.41%) and the highest in spring (4.58±0.35%). The lowest total solid value was obtained in the spring season (11.75±1.80%) which was then increased in winter (12.85±1.96%). Non-fat solid concentration was the lowest in summer (8.07±0.64%) and the highest in autumn (8.94±0.82%). MUN concentration was the highest in summer (8.07±0.64%), and the pH concentration was the highest in spring at 6.93±0.27%. Seasonal variation of SCC and bacterial count were the lowest in spring (0.94±1.54×106 cells/mL and 0.22±0.61×105 CFU/mL, respectively) and was the highest in winter (3.95±7.14×106 cells/mL and 2.23±5.54×104 CFU/mL, respectively).

국내 송아지 분변 내 설사병 병원체 검출과 설사 발병율 조사

김수희 ( Suhee Kim ),강진희 ( Jin-hee Kang ),이창준 ( Chang-joon Lee ),이영성 ( Young-sung Lee ),채정병 ( Jeong-byoung Chae ),강성우 ( Sung-woo Kang ),정숙한 ( Suck-han Jeong ),유도현 ( Do-hyeon Yu ),조아라 ( Ara Jo ),류재규 ( Jae 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.3

Diarrhea is reported as one of the most common diseases in calves. It is thought to be a major cause of productivity and economic loss to cattle producers. The aim of this study is to provide a better under-standing of well-known diarrheagenic pathogens and incidence of diarrhea in Korean calves. In this study, the relationship of calf diarrhea and pathogens were investigated from calves under 60 days of age in five areas of Korea from April to July, 2016. Of examined fecal samples, 38.3% was positive for any pathogens, and Giardia was the most common pathogen (25.5%). The incidence of diarrhea was 31% from pathogen-negative fecal samples whereas 61.1% from pathogen-positive fecal samples, sug-gesting high correlation between pathogenic factor and diarrhea. In addition, 80% of E. coli (K99) pos-itive calves showed diarrhea, suggesting E. coli (K99) could be highly pathogenic. The incidence of diarrhea and infection rate increased with age. Rotavirus was revealed as a major pathogen in calves under 20 days of age, and the infection rate of Giardia increased rapidly in calves 20 to 39 days of age. The information on interconnections between clinical diarrhea and pathogens would contribute to developing strategies for treatment of calf diarrhea.