만성 간질환에서 예후인자로서의 혈청 Fibronectin

백승호(Seung Ho Baick),심창식(Chang Sik Shim),조성원(Sung Won Cho),심찬섭(Chan Sup Shim) 대한내과학회 1988 대한내과학회지 Vol.35 No.3

N/A Fibronectin is a recently characterized high molecular weight opsonic glycoprotein. It5 concentration in plasma has been shown to be reduced in the patients with decompensated liver cirrhosis and fulminant hepatic failure but increased in case of acute hepatitis, fatty liver and chronic hepatitis. We measured the concentration in serum fibronectin in various liver diseases and compared this with the of prothrombin time evel and serum albumin concentration. The results are as follows: 1) There was a statistically significant incrase in serum fibronectin in patients with acute hepatitis and chronic active hepatitis, but a decrease in patients with decompensated liver cirrhosis and hepatoma compared with normal controls. 2) The serum fibronectin concentration was significantly lower in patients with a prothrombin time<the control of 70% or serum albumin<3.0gm/dl than in patients with a prothrombin time>the control of 70% or serum albumin>3.0gm/dl. 3) The serum fibronectin level in patients with liver cirrhosis showed a tendency to decrease more in patients who died than in patients who alived during the period of hospitalization. Thus, we concluded that serum fibronectin concentration may be a good prognostic factor in chronic liver diseases.

위암에서 MDR1 유전자 산물 ( P - glycoprotein ) 및 c - erbB - 2 단백의 과표현과 임상경과와의 관계

김인호(In Ho Kim),원종호(Jong Ho Won),백승호(Seung Ho Baick),진소영(So Young Jin),홍대식(Dae Sik Hong),이동화(Dong Wha Lee),박희숙(Hee Sook Park) 대한내과학회 1996 대한내과학회지 Vol.50 No.3

N/A Objectives: Stomach cancer is the most common cause of death in Korea, The morphological criteria such as histological type, grade, and growth pattern have been used in the assessment of gastric cancer, but appear unable to predict which patients will have a more or less favourable clinical outcome. With the advent of molecular biology, newer methods of classification have become available which may help to overcome the problems associated with morphological parameters and also provide an insight into the underlying mechanisms involved in tumor developement. The aim of this study was to determine the overexpression of the MDR1 gene product(P-glycoprotein) and c-erbB-2 oncogene in the primary gastric cancer, and to analyze its association with clinical parameters and clinical outcome, Methods: The overexpressions of the P-glycoprotein and c-erbH-2 protein by an immunohistochemical staining were evaluated on 54 tissues of gastric cancer patients who were managed with curative gastric resection. Results: The overexpressions of P-glycoprotein and c-erbB-2 protein were observed in 23(43%) and 30(65%) out of 54 tumors. The overexpression of P-glycoprotein showed significant association with depth of invasion of tumor and the overexpression of c-erbH-2 protein showed significant association with histological type of tumor. In univariate analysis, patients with P-glycoprotein positive tumors had poorer survival rates than those with P-glycoprotein negative tumors(p<0.005). There was no relationship between the overexpression of c-erbH-2 protein and survival rate. Multivariate analysis showed that lymph node involvement and depth of invasion were the significant prognostic factor but P-glycoprotein and c-erbH-2 overexpression had no significance. Conclusion: In summary of above findings, the overexpressions of P-glycoprotein and c-erbH-2 protein could be seen in primary gastric cancer and The overexpression of P-glycoprotein showed significant association with depth of invasion of tumor and the overexpression of c-erbH-2 protein showed significant association with histological type of tumor.

재생불량성 빈혈의 병태생리에서 Fas 항원과 Apoptosis의 역할

원종호,이남수,김숙자,정희정,이규택,박성규,백승호,김성일,홍대식,박희숙,Won, Jong-Ho,Lee, Nam-Su,Kim, Sook-Ja,Cheong, Hee-Jeong,Lee, Kyu-Taeg,Park, Seung-Kyu,Baick, Seung-Ho,Kim, Sung-Il,Hong, Dae-Sik,Park, Hee-Sook 대한면역학회 2002 Immune Network Vol.2 No.1

Background: Clinical observations and laboratory studies have supported an immune basis for most acquired aplastic anemias, with the majority of patients responding to immunosuppressive therapy. Fas, a member of the tumor necrosis factor (TNF) receptor superfamily is a critical downregulator of cellular immune responses. Proinflammatory cytokines like interferon gamma (IFN-${\gamma}$) and TNF-${\alpha}$ can induce Fas expression and render hematopoietic progenitor cells susceptible to Fas-induced growth suppression and apoptosis. Methods: In order to investigate the involvement of apoptosis in the pathogenesis of aplastic anemia (AA), we measured the expression of Fas antigen and caspase-3 on bone marrow (BM) mononuclear cells (MNCs) of AA in the presence or absence of IFN-${\gamma}$, TNF-${\alpha}$, or macrophage inflammatory protein 1-${\alpha}$ (MIP-$1{\alpha}$). Results: We confirmed that AA BM MNCs were more apoptotic and highly expressed Fas antigen than normal donors. Stimulation by IFN-${\gamma}$, TNF-${\alpha}$, or MIP-$1{\alpha}$ increased Fas antigen and caspase-3 expression in AA BM MNCs than BM MNCs of normal donors. Anti-Fas monoclonal antibody enhanced IFN-${\gamma}$, TNF-${\alpha}$, or MIP$1{\alpha}$ mediated caspase-3 expression in BM MNCs of normal donors. Among these three cytokines, IFN-${\gamma}$ enhanced apoptosis most strongly via Fas-caspase-3 pathway. Conclusion: These results suggest that Fas signal pathway may play a role in the pathophysiology of aplastic anemia and negative hematopoietic regulators like IFN-${\gamma}$ can induce apoptosis of bone marrow progenitors in part by Fas induction.

생체 외 제대혈 배양에서 거대핵세포 조혈에 대한 Interleukin-11 (IL-11)의 효과

이국경,김찬규,이남수,김숙자,정희정,이규택,박성규,백승호,원종호,홍대식,박희숙,Lee, Kuk-Kyung,Kim, Chan-Kyu,Lee, Nam-Su,Kim, Sook-Ja,Cheong, Hee-Jeong,Lee, Kyu-Tack,Park, Sung-Kyu,Baick, Seung-Ho,Won, Jong-Ho,Hong, Dae-Sik,Park, Hee-So 대한면역학회 2003 Immune Network Vol.3 No.1

Background: The megakaryopoiesis and platelet production is regulated by several hematopoietc factors such as thrombopoietin (TPO), interleukin-11 (IL-11) and interleukin- 3 (IL-3). IL-11 is a potent stimulator of megakaryopoiesis in vivo, and acts primarily as a megakaryocyte maturation factor in vitro and it can act synergistically with IL-3 and TPO. We performed this study to investigate the effects of recombinant human IL-11 (rhIL-11) with other hematopoietic factors on megakaryocyte colony formation in vitro. Methods: CD34+ cells were separated from umbilical cord blood and megakaryocyte colonies using MegaCult Assay Kit were cultured with rhIL-11, recombinant human IL-3 (rhIL-3), and recombinant human TPO (rhTPO) for 7 and 14 days. The number and percentage of CD34+ and CD41a+ cells were determined by flowcytometry. Results: The number of CD41a+ cells were $0.54{\pm}0.05{\times}10^4$ (rhIL-11 100 ng/ml), $5.32{\pm}0.23{\times}10^4$ (rhIL-3 100 ng/ml), and $8.76{\pm}0.15{\times}10^4$ (rhTPO 50 ng/ml) of total expanded cells during the culture of the purified CD34+ cells in liquid phase for 7 days. The number of CD41a+ cells were increased to $7.47{\pm}0.69{\times}10^4$ (rhIL-3+ rhIL-11), $11.92{\pm}0.19{\times}10^4$ (rhTPO+rhIL-11) of total expanded cells, respectively, during the culture of the purified CD34+ cells in liquid phase for 7 days in the presence of rhIL-11 (100 ng/ml). When the purified CD34+ cells were cultured in semisolid mediaincluding various concentration of rhIL-11, the megakaryocyte colonies were not formed. When the purified CD34+ cells were cultured with rhIL-11 and rhTPO or with rhIL-11 and rhIL-3, the number of megakaryocyte colonies were increased compared with rhTPO or rhIL-3 alone. Conclusion: These results indicate that IL-11 exerts a potent proliferative activity to colony forming unit-megakaryocyte from human umbilical cord blood, and it acts with other hematopoietic factors synergistically.

위선암 환자에서 혈장 Vascular Endothelial Growth Factor ( VEGF ) 의 임상적 가치

홍대식(Dae Sik Hong),이규택(Kyu Taek Lee),백승호(Seung Ho Baick),박성규(Sung Kyu Park),원종호(Jong Ho Won),박희숙(Hee Sook Park),이문수(Moon Soo Lee),송옥평(Ok Pyung Song),김창진(Chang Jin Kim) 대한내과학회 1999 대한내과학회지 Vol.56 No.3

N/A Objective : Angiogenesis is an essential step in growth and metastasis of solid tumors. Vascular endothelial growth factor(VEGF) is one of the most important mediators of angiogenesis. VEGF selectively stimulates endothelial cell proliferation and induces angiogenesis. Also VEGF was expressed by several human solid tumors and serum VEGF levels have previously been shown to be raised in patients with breast cancer, gastrointestinal cancer, renal cancer and melanoma. To evaluate the clinical value of plasma VEGF of patients with stomach cancer we studied correlations between plasma VEGF, tumor stage, tumor resection and microvessel invasion by tumor. Methods : VEGF level was measured by ELISA methods in plasmas from 88 patients and after surgical tumor resection from 48 patients with gastric carcinoma. Microvessel staining was done by immunohistochemical staining using anti-CD34 monoclonal antibody on paraffin embedded tissues. Results : The plasma VEGF levels were significantly higher in the stomach cancer patients than in normal controls(p<0.0001). In stomach cancer patients, plasma VEGF level was significantly increased according to stage progression(p<0.05). Moreover early cancer, T1, showed a significantly elevation of plasma VEGF level than that of controls. The level of plasma VEGF fell after surgical resection(n=48) of stomach cancer(p<0.05). Also the plasma VEGF levels were significantly higher in microvessel invasion by tumor(n=25) than in those without invasion(n=19)(p<0.05). Conclusion : In conclusion, plasma VEGF may be useful for predicting disease status and prognosis of patients with stomach cancer.

다발성 골수종 환자에서 kaposi`s sarcoma-associated herpesvirus(KSHV) 감염의 임상적 의의

김찬규(Chan Kyu Kim),홍대식(Dae Sik Hong),박성규(Sung Kyu Park),이규택(Gyu Taeg Lee),원종호(Jong Ho Won),백승호(Seung Ho Baick),이동화(Dong Wha Lee),박희숙(Hee Sook Park) 대한내과학회 2000 대한내과학회지 Vol.58 No.2

N/A Background : Kaposi's sarcoma-associated herpesvirus (KSHV) been shown to be associated with human diseases including Kaposi's sarcoma, pleural effusion lymphoma, multicentric Castleman's disease. The IL-6 may both stimulate myeloma growth and prevent apoptosis of malignant plasma cells. Interestingly, viral IL-6(vIL-6), homolog to human interleukin-6(IL-6) in KSHV genome retains biologic activity. Thus, oncogenic role of the KSHV has been proposed as a pathogenesis of the multiple myeloma. We used ISH to determine the frequency of patients with multiple myeloma and plasmacytosis associated with KSHV-infected BM cells in fresh core biopsies and to determine the correlation between KSHV infection and clinical characteristics. Methods : Bone marrow(BM) biopsy samples from 16 cases of multiple myeloma, 2 cases of monoclonal gammopathy of undetermined significance(MGUS) were obtained from the pathology division of Soon Chun Hyang University Hospital, Seoul, Korea. Biopsy sample of Kaposi's sarcoma for positive control and BM biopsy samples of myelodysplastic syndrome(MDS) and malignant lymphoma for negative control were obtained. Bitinylated probe to KSHV were prepared with the following sequences: 5' to 3' TGCAGCAGCTGTTGGTGTACCACATATCT. and in situ hybridization (ISH) was performed. Results : Among the 18 patients. Two patients were MGUS and among 16 patients with multiple myeloma, 1 in stage IB disease, 1 stage IIB disease, 8 stage IIIA disease, 4 stage IIIB diseases and 2 in variant of multiple myeloma, extramedullary plasmacytoma. Strong positive signal was detected in nuclei and cytoplasm of the malignant cells of biopsy sample from 1 cases of Kaposi's sarcoma by ISH(positive control). Signal was not detected in BM biopsy samples of 7 cases from MDS and malignant lymphoma(negative control). Among 16 patients with multiple myeloma, 15 demonstrated viral positive cells and 2 cases with MGUS also showed viral positive cells by ISH. Signal was detected in nuclei and cytoplasm of stromal cells. Signal was strongly detected in MGUS than multiple myeloma. Positivity of the KSHV was not related with stage of the patients with multiple myeloma. One patients with multiple myeloma was studied at diagnosis and after chemotherapy. After chemotherapy KSHV was not detected. Conclusion : In MGUS and multiple myeloma, KSHV infects the stromal cells of BM rather than malignant plasma cells. On the basis of these data, we have supposed KSHV to play a role in transformation from MGUS to multiple myeloma. Particularly, due to the fact that signal of ISH was strongly detected in MGUS and was not detected in one case with multiple myeloma, it was presumed that KSHV was not major role in already advanced multiple myeloma but statistic significance was not demonstrated because of small numbers of cases. Further studies to reveal the correlation of KSHV and pathogenesis of multiple myeloma are needed.(Korean J Med 58:213-220, 2000)

만성 특발성혈소판감소성자반증에서 부분적 비장색전술의 치료효과

백승호,이규택,이남수,박성규,원종호,홍대식,박희숙,조원수 大韓血液學會 2001 大韓血液學會誌 Vol.36 No.4

비장의 원발성 악성임파종 1예

백승호,백진기,김진오,황성규,조용욱,오도연,양승하 大韓血液學會 1992 大韓血液學會誌 Vol.27 No.2

악성림프종 환자에서 복합화학요법으로 유발된 백혈구 감소증에 대한 인형 재조합 과립구 집락형성-촉진인자(rhG-CSF: Neutrogin)의 효과

박성규,전진우,기신영,유병우,김홍수,백승호,원종호,홍대식,박희숙 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Clinical effects of rhG-CSF were investigated in 17 patients receiving chemotherapy for non-Hodgkin's lymphoma. Patients were given G-CSF 2㎍/㎏/day after 2nd cycle chemotherapy for least 14 consecutive days, and clinical and laboratory results were compared to results of the first cycle. Treatment with G-CSF resulted in increase of mean WBC count(952.9±152.2㎜³vs 1882.4±350.4/㎜³) and neutrophil count(212.9±53.0/㎜³vs 841.5±238.0/㎜³), reduced the duration of leukocytopenic days(12.4±1.3 days vs 5.5±1.0 days, WBC count less than 4000/㎜³) and neutropenic days(4.5±0.9 days vs 2.3±0.7 days, neutrophil count less than 500/㎜³), and reduced the duration of neutropenic fever(5.8±1.1 days vs 0.9±0.5 days). The side effects of G-CSF were not so significant. These results demonstrated that G-CSF is safe and useful for the treatment of neutropenia induced by anticancer chemotherapy of non-Hodgkin's lymphoma and reducing the incidence of febrile periods and duration of antibiotic administration.

폐동정맥루 1예

이충석,백승호,조용욱,오도연,김현태,김용훈,김선주 대한내과학회 1993 대한내과학회지 Vol.44 No.1

순천향 의과대학 천안병원에서 최근 아주 드물게 볼 수 있는 폐동정맥루 1예를 단순 흉부 x선 소견에서 우연히 관찰되어 혈관 조영술로 확진한 폐동정맥루 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Pulmonary arteriovenous fistula (AVF) is a rare congenital condition originated from malformation of capillary development. Dyspnea, hemoptysis, cyuanosis, hemothorax, and severe neurologic complaints may occur because of the dircet entry of unoxygenated, desaturated arterial blood in to the pulmonary venous system, But, most of patients are asymptomatic. The pathogenesis of AVF is incomplete formation of the vascular septae that separate the arterial and venous plexus that anastomose during fetal life. It can occur either as an isolated process or in association with similar abnormalitities of the skin, mucous membrane, hereditary hemorrhagic telangiectasia (Osler-Weber-Rendu syndrome). Recently we experienced a case of the pulmonary arteriovenous fistula which was detected on simple chest x-ray in 56-year-old asymptomatic woman. There was no abnormal connection between fistula and systemic circulation. The confirmative diagnosis of pulmonary AVF was established by pulmonary angiography. Our case was not performed operation due to symptom free and no change of hemodynamic changes.