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새로운 LacZ Gene Fusion 및 Gene Cartridge Vector의 개발에 관한 연구
박교선,이영훈,박충웅,최창진 圓光大學校 基礎自然科學硏究所 1987 基礎科學硏究誌 Vol.6 No.2
In this study, a new lacZ gene vector, plasmid PGEM3-lacZ was constucted, which could be used for the study of the gene regulation and the function of proteins. Plasmid pMC1871 was digested with restriction endonuclease Pst I and Sma I to obtain a DNA fragment with the lacZ-gene(3091 bp). Recombinant plasmid pGEM3-lacZ was constructed by inserting the lacZ gene DNA(Pst I-Sma I cut DNA; 3091 bp) of pMC1871 into the Pst I and Hinc Ⅱ sites of plasmid pGEM3. After then, Recombinant plasmid pGEM3-lacZ DNA was transformated into E. coli HB 101. pGEM3-lacZ DNA/HB 101 was screened on the NZY plate with ampicillin and X-gal(5-Bromo-4-Chloro-3-Indolyl-β-D-Galactoside) by selecting blue colonies. The restriction map of pGEM.3-1acZ DNA was constructed by the following restriction enzymes :Bgl I, Xbal I, Bam HI, EcoR I, Hind Ⅲ, Kpn I, Pst I, Sma Ⅰ, Sal Ⅰ. From the restriction map, the lacZ DNA was confirmed to be present at the expected position in the plasmid pGEM3-lacZ. pGEM3-lacZ/HB101 gives about 10 times more lacZ DNA than pMC 1871/HB101.
비정상 질확대경검사 소견을 보이는 환자에서 환상투열요법의 직접적 적용에 관한 연구
우헌탁,이철민,박교훈,조용균,최훈,김복린,이홍균 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2
Objective: Large Loop Excision of the Transformation Zone (LLETZ) has been widely used for evaluating cervical dysplasia/cancer. We performed this study to evaluate if colposcopically suspected cervical high-grade dysplasia or cancer could be directly evaluated and treated with LLETZ. Methods: 93 women who were scheduled for colposcopic evaluation because of an abnormal cervical cytology report were included in the study in Sanggye Paik Hospital from Jan to Dec 2001. Mean age of the study population was 38.6±9.0. colposcopy directed punch biopsy was not performed and histologic diagnosis was made by LLETZ. Sensitivity and specificity of colposcopic evaluation was calculated and compared with those of cervical cytology. Results: On histopathological examination by LLETZ, 11(11.8%) patients had invasive cancers including 3(3.2%) adenocarcinomas. The sensitivity and specificity of colposcopy for the detection of cervical dysplasia or cancer was 71.7% and 72.3% respectively, wile those of cytology was 56.5% and 74.5% respectively. Conclusion: LLETZ may be adequately performed for the diagnosis and treatment of cervical dysplasia/cancer based on the results of pap smear and colposcopy without punch biopsy. Cost benefit might be anticipated while cost-effectiveness analysis is necessary in the future.
백창렬,이동건,최정현,정현화,조유경,박훈준,이승훈,박윤희,이교영,민우성,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4
As a result of the enlarging pool of unvaccinated children and young adults, there has been an increase in measles in our countries. In these situation, it has been reported that measles associated pneumoinia is easily complicated with fatal respiratory failure, espycially in immunocompromised patients. Herein we report the case of lethal measles pneumonia after allogenic hematopoietic stem cell transplantation in adults proven by autopsy. Recently, one case of measles was encountered in 39-year-old female patients after allogenic bone marrow transplanted case (chronic myelogenous leukemia), who progressed into interstitial pneumonia pattern, despite treatment including antibiotics, immunoglobulin. The patient died of giant cell pneumonia compatible with that of measles which was comfirmed in the section of necropsy lung specimen. (Korean J Infect Dis 33:301∼309, 2001)
경기도 북부지역 아파트의 지하주차장 실내공기질 변동 특성
박현구(Hyun-Ku Park),정연훈(Yeon-Hoon Jung),김병록( Byeong-Lok Kim),김진길(Jin-gil Kim),신형순(Hyung-Soon Shin),이상수(Sang-Soo Lee),오조교(Jo-Kyo Oh) 한국환경보건학회 2018 한국환경보건학회지 Vol.44 No.3
Objectives: The study examined indoor air quality during daytime and commute times in underground parking lots in five apartments located in northern Gyeonggi province. Methods: This study examined the temporal characteristics of PM 10 , CO 2 , CO, HCHO and VOCs in the indoor air quality of the underground parking lots in five apartments. concentration in daytime were ranged from 37.4 to 69.9 µg/m 3 which complied with the indoor air quality maintenance standard (200 µg/m 3 ) and Gyeonggi province ordinance standard (180 µg/m 3 ). However PM 10 concentrations in commuting time were in range of 447.3~944.0 µg/m 3 that exceeded for both criteria. The CO 2 , CO and HCHO concentrations complied with the standard. The VOCs indicated high tendency in the order of toluene, xylene, ethylbenzene and benzene. This tendency was more apparent in commuting time than daytime. Conclusion: All indoor parking lots of five apartments were equipped with ventilators. However the most apartment residents did not operate blowers to save electricity and the lack of interest for indoor air quality. In order to improve the indoor air quality of underground parking lots in apartment, operating ventilation system during commuting time is recommended.
Park, Seung-Taeck,Park, Yang-Kyu,Park, Jae-Hwang,Cho, Kwang-Ho,Ryu, Do-Gon,Jeon, Byung-Hoon,Shin, Min-Kyo,Han, Du-Seok,Cho, Nam-Su,Shin, Dong-Min Kyung Hee Oriental Medicine Research Center 2000 International journal of oriental medicine Vol.1 No.1
Effects of Rhizoma gastrodiae on glucose oxidase-induced neurotoxicity was investigated in cultured newborn mouse spinal dorsal root ganglion(DRG) neurons that were treated in the media with or without glucose oxidase. In addition, the protective effect of Rhizoma gastrodiae extract against glucose oxidase-induced neurotoxicity was examined. Cytotoxic values were expressed as a percentage of number of living cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In this paper, exposure of neurons to glucose oxidase resulted in a significant call death in a dose- and time-dependent manners in DRG neuron cultures. The decrease in cell viability induced by the glucose oxidase was blocked by Rhizoma gastrodiae extract. These results indicate that the neuroprotective effect of Rhizoma gastrodiae extract against glucose oxidase-induced neurotoxicity may result from a prevention or attenuation of oxidative damage induced by glucose oxidase.