Phospholipase A₂활성억제와 관련한 시호 물추출액 및 시호 약침액의 항염증효능에 관한 연구

문진영,남경수,임종국 동국대학교 경주대학 1997 東國論集 Vol.16 No.2

농도별 시호 약침액과 시호 물 추출액을 대상으로 phospholipase A_2(PLA_2)에 대한 시호의 영향을 알아보기 위하여, 1-stearoyl-2-[1-^14C]arachidonyl-sn-glycerophosphocholine을 기질로 하여 Ⅰ형 PLA_2(porcine pancreas) 및 Ⅱ형 PLA_2(Crotalus atrox)의 활성에 대한 효과를 측정하여 다음과 같은 결과를 얻었다. 1) PLA_2 효소의 활성발현에 최적 배양시간이 어떠한지를 알아보기위해 pH 7.0에서 37℃에서 반응시간을 0분에서 180분간의 범위까지 기질의 농도를 20n㏖로 하여 porcine pancreas 및 Crotalus atrox 유래의 PLA_2를 대상으로 활성의 변화를 관찰한 결과, 반응시간이 길어질수록 두 효소의 활성이 점차로 증가하였다. 2) Porcine pancreas 유래의 Ⅰ형 PLA_2의 활성에 미치는 시호의 영향을 관찰한 결과, 시호 약침액은 최고 92%, 시호 물 추출액에서는 최고 94%의 PLA_2 활성 저해효과를 보였으며, 희석배수가 증가할수록 활성 저해효과는 감소하였다. 3) Crotalus atrox 유래의 Ⅱ형 PLA_2의 활성에 미치는 시호의 영향을 관찰한 결과, 시호 약침액은 최고 60%, 시호 물 추출액에서는 최고 55%의 PLA_2 활성 저해효과를 보였다. 또한 시호 약침액 및 물 추출액의 원액에서 4배 희석액까지는 40% 이상의 억제효능을 보인 반면, 8배 이상의 희석액부터는 활성 억제효능이 급격히 감소하였다. 이러한 결과로 미루어 볼 때 시호 약침액 및 시호 물 추출액은 모두 PLA_2에 대한 강한 억제활성이 있음을 알 수 있었다. 또한 PLA_2 효소의 유형별로 볼 때, 시호는 Ⅱ형 PLA_2에 대해서 보다 Ⅰ형 PLA_2에 대한 억제효능이 훨씬 강함을 알 수 있었다. 그리고 시호 약침액과 시호 물 추출액을 비교해보면 Ⅰ형 PLA_2에 대해서는 시호 물 추출액이, Ⅱ형 PLA_2에 대해서는 시호 약침액이 상대적으로 다소 강한 억제효능이 있음을 알 수 있었다. 본 연구의 결과에서 시호는 그 효능적 측면에서 항 염증제, 특히 PLA_2 저해제로 개발될 수 있는 가능성이 제시된다. Phospholipase A_2(PLA_2) is a key enzyme that catalyze the hydrolysis of the ester at the sn-2 position of cell membrane phospholipid to produce a lysophospholipid and arachidonic acid. Lysophospholipid is the precursor of platelet-activating factor(PAF). And arachidonic acid is the substrate for cyclooxygenase and lipoxygenase in the arachidonic acid cascade generating various eiconanoids such as prostaglandins, leukotrienes and thromboxanes. Thus, PLA_2 plays an important role in the pathogenesis of inflammation. Recently, compounds that inhibit PLA_2 activity have been received considerable attention as potential therapeutic agents in the treatment of inflammation. Bupleury radix has been used as a natural drug for fever, inflammation and liver disease in traditional medicine. This study was performed in order to investigate the inhibitory effects of Bupleury radix aqueous extract(BRAE) and aqua-acupuncture solution(BRAA) on activities of two different types of PLA_2. BRAE showed about 94% inactivation of group Ⅰ PLA_2 from porcine pancreas, and 55% inactivation of group Ⅱ PLA_2 from Crotalus atrox ultimately. And BRAA exhibited about 92% inactivation of group Ⅰ PLA_2, and 60% inactivation of group Ⅱ PLA_2. On the basis of these results, it is suggested that Bupleury radix might play a anti-inflammatory role by a mechanism that probably involves inactivation of PLA_2.

柴胡가 free radical에 의한 脂質過酸化物 生成에 미치는 效果

문진영,최미정,남경수,임종국 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

Antioxidant effects of aqueous Siho extract on lipid peroxidation were determined by TBA value and also by lipid peroxidation value during the autoxidation of Iinoleic acid. Siho extracts exhibited markedly antioxidant aCtivity, which inhibited 99% of linoleic acid peroxidation. And Siho extracts showed 42% scavenging effect on α,α-dlphenyl-β-pjcrylhydrazyl radical, also exhibited a 60-94% inhibitory effect on superoxide generation. Siho extracts dose-dependently protected the cell death induced by tert-butyl hydroperoxide (t-BHP) and significantly increased cell viability In the normal rat liver cell(Ac2F) . These results suggested that Siho might play a protective role in lipid peroxidation by free radicals. Key Words: lipid peroxidation ; Siho extracts; scavenging effect

백서에서 신 허혈성 손상에 미치는 칼슘 길항제의 효과

문철웅,정종훈,박천국,이승일,배학연,장경식,김만우,정춘해,홍순표,이병래,김호중 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Renal ischemia is one of the most common causes of acute renal failure. Four factors related to the pathogenesis of acute renal failure are vasoconstriction, decreased glomerular filtration rate, tubular back leak of filtrate, and intratubular obstruction. The cellular response to renal ischemic insults include decreased content of adenosine trihosphate, lipid peroxidation induced membrane degradation, alteration in cellular pH, and calcium or phospholipase induced mitochondrial dysfunction. Much attention has been given to the role of increased cellular calcium as a pathogenetic contributor to cell injury during ischemia. Author studied the protective effects of calcium antagonists on cellular injury during renal ischemia in rat. To investigate the protective role of these agents, author measured the amount of malondialdehyde(MDA) and the enzyme activities of free radical scarvengers-superoxide dismutase(SOD), catalase and glutathione peroxidase from non-pretreated group and calcium antagonists pretreated groups in control, ischemia and reflow subgroups. The results were summerized as follows: 1) The amount of MDA in non-pretreated group was higher in the reflow compared with the control(<p<0.01). But, in all pretreated groups, there was no statistically difference in the amount of MDA. 2) The SOD activity in non-pretreated group was lower in both the ischemia and the reflow compared with the control (P<0.05). But, in both verapamil and trifluoperazine-pretreated groups, there was no statistically difference in the SOD activity. 3) Both catalase and glutathione peroxidase activities in non-pretreated group were lower in both the ischemia and the reflow compared with the control (P<0.05). But in all pretreated groups, there was no statically difference in both catalase and glutathione peroxidase activities. These results suggest that free radical mediated ischemic injury by renal artery clamp in rat can be protected by intraperitoneal pretreatment with calcium antagonists. As trifluoperazine has a protective effect in renal ischemia, the calcium activated calmodulin dependent enzyme may play a role in renal ischemic injury.

간수, 기문혈의 당귀 약침자극이 acetaminophen으로 유발된 흰쥐의 손상간에 미치는 영향

박경미,문진영,안준철,최미정,남경수,임종국 동국대학교 한의학연구소 1996 東國韓醫學硏究所論文集 Vol.5 No.-

This study was done in order to investigate the protective effects of A.G.R.(Angelicae gigantis Radix) aqua-acupuncture on acetaminophen induced liver damage in rats. The liver damage was induced by acetaminophen (500mg/kg) injection into the peritoneum. The A.G.R. aqua-acupuncture solution was injected into the corresponding loci to Ganshu(BL_(18)) and Qimen(LR_(14)) of human body and a blank locus of the root of tail on four consecutive times at 0, 3, 6, and 12 hours after acetaminophen injection. And the serum GOT, GPT, LDH, ALP activities, total bilirubin, direct bilirubin levels were measured in the rats, The serum GOT, GPT, LDH ALP activities and bilirubin level were decreased comparing with that of a control group in case of A.G.R. aqua-acupuncture treated group, specially Ganshu and Qimen aqua-acupuncture treated groups showed an obvious significant decrease.

標幽賦의 海穴에 對한 文獻的 考察

孫榮晙,文振榮,林鍾國 동국대학교 한의학연구소 1995 東國韓醫學硏究所論文集 Vol.4 No.-

The aim of this studies was to elucidate the meaning, theoretical propriety and use of the Sea points in PYOYOUBU. The results obtained were as follows ; We reached a conclusion that Sea points, that is to say Xuehai(SP_(10)), Xiaohai(Sl_(8)), Shaohai(HT₃), Zhaohai(KI_(6)), Qihai (CV_(6)) were gathering places of meridian energy. And the remedial function of Sea points against visera and bowel disease was closely connected with it's meridian pointological naming. Besides the remedial function of Sea points like this was closely connected with the cases in literature cited.

馬丹陽天星十二穴에 對한 文獻的 考察

朴慶美,文振榮,林種國 동국대학교 한의학연구소 1995 東國韓醫學硏究所論文集 Vol.4 No.-

The aim of this studies was to elucidate the derivation, meaning and use of the special points of MADANYANG. The results obtained were as follows : The remedial cases of spacial points of MADANYANG came within the category in that of literature cited. So, it was considerable that a method of selection of remedial points in MADANYANG was not creative but universal at that time. The special points of MADANYANG did not have a fixed organizing rule against it's medrian, visera and bowel in contrast to source points, connecting points, five element point etc. And it was only organized on the basis of remedial value.

산모혈과 제대혈에서 연농도에 관한 조사

이덕희,문종국,배강우,이용환,김진하,박강원 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.3

This study was conducted to evaluate the blood lead levels in 141 pregnant women who were not occupationally exposed to lead and their umbilical cord, and to identify some differences in blood lead levels by living area. The subjects were all delivered at St. Benedict Hospital in Pusan and the blood lead levels were measured by graphite furance atomic absorption spectrophotometer. The summarized results were as follows. 1. The geometric mean lead levels in maternal and cord blood were 8.0±1.8μg/dl, 5.0±0.6μg/dl respectively. 2. Lead levels in maternal(8.7±1.7μg/dl) and cord blood(5.4μ±0.7μg/dl) of industrial area(n=24) were higher than residential area(n=124) which were 7.9±1.7μg/dl and 5.0±0.6μg/dl respectively(p<0.05). 3. Maternal blood lead concentration was significantly correlated to cord blood lead concentration(r=0.6090, p<0.001), but other variables(maternal age, paternal smoking, birth weight, etc) were not correlated.

Distribution of chitinases and characterization of two chitinolytic enzymes from one-year-old Korean Ginseng (Panax ginseng C.A. Meyer) roots

( Jong Kook Moon ),( Beom Ku Han ),( T. Doohun Kim ),( Do Hyun Jo ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.11

We report the tissue-specific distribution of chitinolytic activity in Korean ginseng root and characterize two 31-kDa chitinolytic enzymes. These two enzymes (SBF1 and SBF2) were purified 70- and 81-fold with yields of 0.75 and 1.25%, respectively, and exhibited optimal pH and temperature ranges of 5.0-5.5 and 40-50℃. With [3H]-chitin as a substrate, Km and Vmax values of SBF1 were 4.6 mM and 220 mmol/mg-protein/h, respectively, while those of SBF2 were 7.14 mM and 287 mmol/mg-protein/h. The purified enzymes showed markedly less activity with p-nitrophenyl-N-acetylglucosaminide and fluorescent 4-methylumbelliferyl glycosides of D-N-acetylglucosamine oligomers than with [3H]-chitin. End-product inhibition of both enzymes demonstrated that both are endochitinases with different N-acetylglucosaminidase activity. Furthermore, the NH2-terminal sequence of SBF1 showed a high degree of homology with other plant chitinases whereas the NH2-terminal amino acid of SBF2 was blocked. [BMB reports 2010; 43(11): 726-731]

흰쥐에서 톨루엔이 요중 벤젠 대사 물질인 phenol과 S-phenylmercapturic acid 배설에 미치는 영향

문종국(Jong Kook Moon),김치년(Chi-Nyon Kim),노재훈(Jae Hoon Roh) 대한직업환경의학회 2002 대한직업환경의학회지 Vol.14 No.2

스마트 시티 도시 서비스의 수요와 만족도를 고려한 서비스 제공방안에 관한 기초연구

문종국(Moon jong-kook),조병호(Jo byung-ho),최봉문(Choi bong-moon) 한국콘텐츠학회 2017 한국콘텐츠학회 종합학술대회 논문집 Vol.2017 No.5