보조자극분자를 이용한 암치료

장미라,윤희령,성인옥,정상원,김용찬,손정희,이혜영,강광일,백상기,김영상 충남대학교 생물공학연구소 2002 생물공학연구지 Vol.8 No.1

Tumor cells express tumor-associated antigen which can be target to the cytotoxic Tlymphocytes. CTL precursor activation requires antigen recognition and cytokine help from Th cells. In this pathway, the cross-presentation mechanism, professional antigen presenting cells first pick up tumor antigen and present the antigen to Th cells. Another pathway is to activate CTL precursor by the direct priming. The tumor cells, however, lack costimulatory signal, which is required for full activation. The lymphocytes recognized specific antigen without costimulatory signal resulted in anergy. Tumor cells engineered to express the costimulatory molecules are better immunogen and induce antitumor immunity. We will discuss the recent progress in tumor therapy using the costimulatory molecules on the basis of the direct priming mechanism.

下體部의 動作에 따른 Slacks의 製作時 여유분량에 관한 硏究

金恩姬,咸玉相 啓明大學校生活科學硏究所 1992 科學論集 Vol.18 No.-

To establish a marginal part of slacks in accordance with motion and habitus, I hereby classified the married woman in their thirties by three habitus : thin, standard, and fat. Then affer selecting a standard classified by each habitus I studied the variation of body surface and the cross-cut method. I accordingly conducted a functional test by making a standard clothes of which the reasonable marginal parts-1cm, 1.5cm, 2cm, 2.5cm-had been established. The results of the test are as follows; 1. Concerning the variation of body surface, the variation rate increased when the habitus became bigger, and the width of variation did when the radius of the articutation movement became larger. The widthe of increase classified by parts showed a considerable increase in Hip Girth, Thigh Girth, and Midle Waist Girth-the horizontal items-of habitus A, B, C. In the vertical items, Back Line, Front Line and Center Back Line showed a considerable increase. But Later Line showed a constraction in all movements. When marking proper slacks, the establishment of marginal part of Hip Girth in accordance with the habitus should be considered. 2. Concerning the analysis of space variation of cross-cut, the space variation increased when the movement became larger and the habitus became bigger. 3. As a result of the comparison between the variation of body surface and the space variation in Cross-cut method, the variation was remarkable when the movement became larger, and the length of slacks became longer. But when putting on clothes, the Cross-cut method having a restriction on articulation movement showed a considerable variation in measures. In consideration of the a fore-mentioned results, when making clothes, the establishment of marginal part, which took the fact that there is an interrelation between movement and wearing clothes because the restriction on articulation causes the decrease in a radius of movement when wearing clothes into serious consideration, is necessary. 4. The result of functional test; Habitus A : The test clothes having marginal part of 1㎝ was rated good, getting 4.74marks in appearance test, 4.36marks in functional test. Habitus B : The test clothes having marginal part of 1.5cm was also rated good, getting 4.88marks in appearance test, 4.42marks in functional test. Habitus C : The test clothes having marginal part of 2cm was rated best, getting 4.70marks in appearance test. The test clothes having marginal part of 2.5cm got 4.30marks in functional test. In conclusion the establishment of marginal part of 0.5 to 1㎝ universally applicable in making a standard slacks causes the married women in their thirties to feel inconvenience on the parts of Center Front Line, Hip and Belly in functional and beautiful in silhouette as possible, the appropriate establishment of marginal parts assified by each habitus in essential.

돈지, 들깨유 및 달맞이꽃 종자유의 혼합급이가 흰쥐의 혈청 지질 성분에 미치는 영향

김성희,김한수,강정옥,박필숙,정승용 慶尙大學校 1992 論文集 Vol.31 No.2

돈지 및 들깨유와 달맞이꽃 종자유등 2종 식물 종자유아의 혼합비율을 달리한 유지의 급이가 흰쥐의 혈청 지질성분에 미치는 영향을 구명하기 위해 S·D계 숫쥐에게 돈지 2.5% 및 들깨유와 달맞이꽃 종자유의 혼합비율을 달리하여 4주간 급이 사육한 후 혈청의 지질 성분을 분석 검토한 결과는 다음과 같다. 혈청의 총콜레스테롤 농도는 대조군(A군 돈지10%)에 비해 B군(들깨유7.5%+돈지2.5%)이 유의적으로 낮았으며, HDL-콜레스테롤 농도는 대조군에 비해 C군(들깨유 5.0%+달맞이꽃 종자유2.5%+돈자2.5%), D군(들깨유 2.5%+달맞이꽃 종자유5.0%+돈지2.5%)및 E군(달맞이꽃 종자유7.5%+돈자2.5%)이 높았고, 총콜레스테롤에 대한 HDL-콜레스테롤의 농도비는 대조군에 비해 E군이 현저하게 높았다. 콜레스테롤를 급이군은 콜레스테롤 무급이군에 비해 전 군에 걸쳐 총콜레스테롤 농도는 월등히 높았고 HDL-콜레스테롤 농도는 낮았다. 콜레스테롤 에스테르의 농도는 대조군에 비해 B및 C군은 낮은 반면, D및 E군은 높았고 콜레스테롤 급이군에 비해서는 대조군에 비해 전군이 유의적으로 낮았다. LDL및 LDL-콜레스테롤 농도는 대조군에 비해 전 군이 유의적으로 낮았으며 B군은 특히 현저하게 낮은 경향을 나타내었고, 또한 콜레스테롤 급이군은 콜레스테롤를 무급이군과 유사한 경향을 보였으나 전반적으로 월등하게 높았다. 인지질 및 중성지질의 농도는 대조군에 비해 전 군이 유의적으로 낮게 나타났고, 콜레스테롤 급이군의 혈청 중 인지질 및 중성지질의 농도는 콜레스테롤 무급이군고 유사한 경향이였으나 전반적으로 보아 현저하게 낮았다. In order to observe the effects of the feeds mixed with the lard and two vegetable seeds oils on lipid components of serum in rats, the oils mixed with 2.5% lard and various level of perilla oil and evening primrose oil were administered to the male rats of the S.D. for 4 weeks. Total cholesterol concentrations of serum were significantly lower in the B group(perilla oil 7.5%+ lard 2.5%) than in the control group(A group ,lard 10%). HDL-cho-lesterol concentrations in serum were higher in the C(perilla oil 5.0%+evening primrose oil 2.5%+lard 2.5%), D(perilla oil 2.5%+evening primrose oil 5.0%+lard 2.5%) and E(evening primrose oil 7.5%+lard 2.5%) groups than in the control group. In the supplemeted cholesterol groups(SC groups), total cholesterol concentrations in serum were by far higher and HDL-cholesterol concentrations in serum were lower than in the nonsupplemented cholesterol groups(NSC groups). Cholesteryl ester concentrations in serum were lower in the B and C groups, but higher in the D and E groups than in the control group. In the SC groups, cholesteryl ester concentrations in serum were significantly lower in the other groups than in the control group. concentrations of LDL-cholesterol in serum were lower in the other groups and remarkably in SC groups were similar to the NSC groups, but those of serum were by far higher in all groups. Concentrations of phospholipid and triglyceride in serum were significantly lower in the other groups than in the control group, in the SC groups, concentrations of phospholipid and triglyceride were similar to the NSC groups, but those from serum were remarkably lower in all groups.

유두상 갑상선 암에서 p21, p53 및 증식세포 핵항원(PCNA)의 예후인자로서의 유용성

김보균,서성옥 고려대학교 의과대학 1997 고려대 의대 잡지 Vol.34 No.1

There were many studies t o show that p21. p53 and PCNA overexpression were known to be predictive factors for human cancer. The purpose of this study was to evaluate whether these molecular oncogenes were useful as prognostic factors in patient with papillary thyroid carcinoma. Using the monoclonal antibodies MAb Y13-259, PAb 1801 and PClO against each oncogene protein studied, the avidin-biotin peroxidase complex method provided immunohisto-chemical staining of bound oncogene protein for p21-ras, p53 and PCNA, respectively. Analysis were made on paraffin-embedded pathology tissues of 58 patients with papillary thyroid cancer who underwent thyroidectomy in Korea University Anam Hospital from January 1984 to December 1992. Forty-six patients were women (79.3%) and 12 were men (21.7%), with mean age 42.0±19.2 years. Patients were divided into groups based on postoperative tumor recurrence. Eleven patients had recurrence of disease ; 47 remained free of loco-regional or distant metastasis at mean follow-up of 74.3 months(range 36 142 months). The positive rates of p21. p53 and PCNA were 22.2% (13 of 56), 45.6% (26 of 57) and 70.9% (39 of 55) respectively. No significant differences were observed in the age and sex of patients with p21, p53 and PCX4 positive and negative tumors. Using stastical analysis, there was no significant difference in p21, p53 and PCNA stains between recurrent and non-recurrent groups (p>0.05). while AMES score (p=0.042). the tumor size (p=0.005). capsular invasion(p=0.003) and lymph node metastasis(p=0.002) were significantly correlated with tumor recurrence. Conclusion : This study shows that expression of p21, p53, PCNA are not useful as prognostic factors in papillary thyroid cancer.

대추 물추출물이 Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 영향

金尙玉 영남이공대학 1997 論文集 Vol.26 No.-

대추 물추풀물이 B(a)P에 의해 유도된 간 손상에 미치는 영향을 알아보기 위하여 흰쥐에게 1 주일간 대추 추출물을 1 일 1 회 경구투여하고 B(a)P을 대추 물추출물을 투여한 마지막 날에 1 회 복강주사하여 효소 활성도와 지질 함량 변화를 측정하고 MTT assay를 수행한 결과는 다음과 같다. In vitro 실험에서 B(a)P은 간세포에 대해 현저한 세포독성을 나타내었으며 대추 물추출물은 이러한 세포독성을 정상수준으로 유의성 있게 회복시켰다. 또한 in vitro 실험에서도 B(a)P의 투여는 효소 활성도와 지질 함량을 유의성있게 상승시켰고 대추 추출물은 이러한 상승을 유의성있게 감소시켰다. 따라서 대추 물추출물은 in vitro와 in vivo에서 공(共)히 B(a)P으로 인한 간손상에 뚜렷한 보호효과를 나타내었는데 이는 대추 추출물이 간의 저항력 및 간세포 기능을 유지시키므로써 보간작용을 한 것으로 사료된다. The protective effect of jujube water extract on beizo(a)pyrenc induced liver injury was studied in rats in vitro and in vivo. In vitro experiment, primary cultured hepatocytes(5×105 cells/㎖) were cultured for 20-24 hours after adding jujube water extract(625 ㎍/㎖) and B(a)P(50 μM) in culture medium. In vivo experiment, jujube water extract(0.25 g/㎏ body weight/day, per os) was administered for 7 days and B(a)P(0.1 ㎎/㎏ body weight, intraperitoneally) was given to the rats after the last administration of extract Jujube water extract significantly recovered enzyme activities(GOT, GPT, LDH and ALP) and lipid contents(total cholesterol, triglyceride and IIDL-cholesterol) changed by benzo(a)pyrene(B(a)P) in vivo. In vitro experiment, as a result of 3-(4,5-dimethylthiazol-2-yD-2,5-diphenyl tetrazolium brotnide(MTT) assay jujube water extract stimulated hcpatocyte proliferation. And it significantly recovered enzyme activities(GOT, GPT and LDH) in comparison to group Ⅱ administered B(a)P only. It was suggested that jujube water extract have a protective effect on liver injury by B(a)P.

어휘획득 전략 훈련프로그램이 초등학교 학습장애 학생의 어휘력 신장과 자기효능감에 미치는 영향

김성숙,김윤옥 국립특수교육원 2008 특수교육연구 Vol.15 No.2

본 연구는 어휘획득전략 훈련프로그램이 초등학교 학습장애학생의 어휘력 신장과 자기효능감에 미치는 효과를 검증하고자 실시되었다. 연구에 참여한 대상은 충북에 소재한 초등학교 6학년 학습장애학생 3명이었다. 연구자가 제작한 어휘력 검사지를 이용하여 학생들의 어휘 활용 능력을 측정하였으며, 자기효능감 수준도 함께 검사하였다. 본 연구에서는 어휘획득전략을 전략교수 8단계 과정을 통해 적용하였으며, 단일대상연구의 대상자간 중다 기초선 설계를 이용하여 전략 교수의 효과를 검증하였다. 연구 결과, 학습장애학생들이 어휘획득전략을 완전학습 할 수 있고 전략 프로그램이 어휘력 신장과 자기효능감을 향상시킬 수 있는 것으로 나타났다. 그리고 어휘력을 신장시킬 수 있도록 돕는 어휘획득전략이 전반적인 모든 교과에 파급되어 학업 성취도를 향상시키는데 필요한 자기효능감 기여 정도와 고학년 학습장애 뿐만 아니라 저학년 학습장애학생과 학습부진아 학생에게도 적용하여 어휘력을 신장시키는데 기여할 수 있는지에 대해 논의하였다. The purpose of this study was to verify the effects of a Vocabulary Acquisition Strategy, applied to the Elementary students with learning disabilities (LD) on the Vocabulary Expansion and Self-efficacy. The participants in these study were three children with LD in 6th Grade Elementary School, Goesan Chungbuk. This study was started on March 2th, 2007 until October 28th, 2007 with 28 to 32 sessions. The subjects were tested by a 'Vocabulary Acquisition Strategy' tests that developed by Youn Ock Kim(2006) to measure children's ability to utilize strategies on vocabulary acquisition. The Vocabulary Expansion and self-efficacy were also measured. The 'Vocabulary Acquisition Strategies' applied through 8 steps of strategy teaching, and the researcher examined the effects of the strategy using a multiple baseline design across the subjects. The followings were the results of this study: First, students with LD could reach 'Vocabulary Acquisition Strategies' learning up to the mastery level. The researcher guided these subjects to pass the Strategy learning processes step by step. He offered positive feedbacks and helped them, to correct their errors. So that, they could master the strategies. Second, the Vocabulary Acquisition Strategies improved the targeted students' using Vocabulary Expansion method. Third, the strategies improved the students self-efficacy. the students developed their ability to do what is supposed to do. The findings in this study showed that the Vocabulary Acquisition Strategies had positively influenced the Vocabulary Expansion and Self-efficacy of Elementary students with LD. It has some implications that, the future studies employing the Strategy should be designed with wider range of region and more diverse graders. Further, more it is needed to support students in connection with their home in order to maintain the effectiveness of the Strategy. Teachers need to collaborate the parents of the students with LD to help them generalize and use the Strategy continuously.

Spiroxazine유도체의 분자 광스위칭을 이용한 능동형 평판 광도파로 자외선센서

옥경식,김시홍,김성훈,김응수,고광락,강신원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

UV Sensor is widely used for various fields with the study on UV detector and UV related researches. Also due to the recent increasing of UV radiation by ozone depletion enforces the development of techniques on efficient UV detection. Thus, in our study, we proposed novel UV sensor using photochromic molecular device that has good UV sensitivity and easiness to manufacturing, and low cost. Furthermore, this molecular device is useful for the construction of a thin film active waveguide type device that has excellent sensitivity and feasibility to miniaturization.

김치숙성과 품질에 미치는 건어물의 첨가 효과

김상옥,이윤경 영남이공대학 산업기술연구소 2000 産業技術硏究 Vol.12 No.-

Dried fish extracts on the fermentation and quality of kimchi was investigated. Dried pollack, squid, octopus, seafish, filefish, shrimp, cod and anchovy were extracted with boiled water. The extracts supplemented 2% as a basis of dried fish in kimchi. pH, acidity, sensory quality and texture as quality indicator were investigated. The decrease in pH and increase in acidity during fermentation were lower in the dried fish extracts-supplemented kimchi than in control products. Sensory qualtiy of the kimchi with shrimp extract-supplemented kimchi among the dried fish extracts, showed the highest scores in sour taste, crispness and overall taste. Hardness of kimchi was also higher in the kimchi with shrimp extract than in control products.

오미자 클로로포름 추출액이 흰쥐에 있어서 Benzo(a)pyrene에 의해 유도된 간장해에 미치는 영향

김상옥 영남이공대학 1995 論文集 Vol.24 No.-

The protective effect of omija chloroform extract on benzo(a)pyrene-induced liver injury was studied in rats in vitro and in vivo. In vitro experiment, primary cultured hepatocytes(5x10^(5) cells/㎖) were cultured for 20-24 hours after adding omija chloroform extract(5.1 ㎍/㎖) and B(a)P(50 μ/M) in culture medium. In vivo experiment, omija chloroform extract(0.1 g/㎏ body weight/day, per os) was administered for 7 days and B(a)P(0.1 ㎎/㎏ body weight, intraoeritoneally) was given to the rats after the last administration of extract. Omija chloroform extract significantly recovered enzyme activities(GOT, GPT, LDH and ALP) and lipid contents(total cholesterol, triglyceride and HDL-cholesterol) changed by benzo ( a ) pyrene ( B ( a) P )in vivo. In vitro experiment omija chloroform extract didn't stimulate hepatocyte proliferation but significantly recovered enzyme activities(GOT, GPT and LDH) in comparison to group Ⅱ administered B(a)P only. It was suggested that omija chloroform extract have a protective effect on liver injury by B(a)P.

고로슬래그를 굵은골재로 사용한 콘크리트강도에 관한 연구

김영인,고창옥,이채규,양승현 조선대학교 국토개발연구소 1994 국토개발연구 Vol.14 No.2

Blast furnace, by-product of iron industry has widely used as reclamation materials for pavement or harbour construction. The studies which make blast furnace slag use as aggregates for concrete have being actived owing to natural aggregates insufficiency. In order to use air cooled blast furnace slag as a coarse aggregate for concrete which was fixed to 440kg, 400kg, 360kg, 320kg, 280kg, 240kg of cement content were performed. This study is aimed to ascertain the feasibility of utilizing the concreate with crushed slag aggregates and to find specific properties such as slump air content, compresive strength, tensile strength, bending strength. The results of this study are summarised as follows; 1. On the base of 320kg of unit cement weight the strength according to each dry day are similar in the condition of 45% of w/c whereas in the condition of 50% of w/c the strength of 7-days are similar, but the strength of 28-days are different. 2. In the relation of δ25 - c/w, the strength of slag concrete is greater than that of crushed storn concrete in condition of epual c/w except 400kg/㎥. 3. Slag of concrete as aggregate is destoryed with mortar. 4. The relation of δ28 and tensile strength is very good(γ = 0.8), the equation of regression analysis is δt= 0.63+0.00598 × 28. 5. The reation of bend strength and w/c is liner(γ = 0.72) the equation of regression analysis is δb=80-0.69w/c. 6. It must be care to abtain equal workability to natual aggregate concrete because the change of water absorption is greate. The water content of slag concrete is more than that of crushed storn concrete to obtain the same workability. It must be careful to use blast furnace slag as a coarse aggregate for concrete. And after investgating the quality of demended concrete carefully, real mixing work must be done.