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박미숙,이명수,정영자,김혜정,문성록,김용규 한국스포츠심리학회 2003 한국스포츠심리학회지 Vol.14 No.3
본 연구는 스트레스 반응을 측정하는 스트레스 반응 척도 설문지(Symptoms of Stress: SOS)를 이용한 횡단적 연구로서 심신수련의 하나인 기수련이 스트레스 반응에 미치는 효과를 살펴보았다. 72명의 건강한 정상인과 180명의 기수련자를 대상으로 하여 조사하였으며, 기수련자들은 수련기간에 따라 초급군(1-4개월; 64명), 중급군(5-12개월; 50명), 고급군(1년 이상; 66명)로 나누었다. 실험결과 1년 이상 수련한 기수련자들이 말초혈관 증상군, 심폐 증상군, 중추신경계 증상군, 위장계증상군, 습관적 행동 형태군, 우울 증상군, 불안증상군등의 스트레스 반응 하위척도에서 대조군에 비해 통계적으로 유의하게 감소하였다. 전체 스트레스 값은 대조군에 비해 기수련자 집단이 아주 낮았으며 수련기간이 길수록 감소하였다. 이러한 결과는 기수련이 스트레스 중재와 스트레스 증상을 줄이는데 효과가 있음을 나타낸다. We examined the effects of Qi-training, one of Korean traditional psychosomatic training, on stress coping ability by cross-sectional investigation with inventory of symptoms of stress (SOS). Four types of groups- normal healthy (n-74), three groups of Qi-trainees (group primary level: subjects with Qi-training for 1-4months, n=64; middle level: 5-12 months, n=50; high level: more than 12 months, n=66) participated in this study, Qi-trainees with over 1 year training had significantly lower scores in all SOS subscales compared to controls. Total stress scores of Qi-trainee groups were substantially lower than controls and further reduction with larger training periods. These results suggest Qi-training is effective in stress management and decreasing symptoms of stress
Moon, Hae-Jeong,Baek, Dong-Won,Lee, Bo-Young,D. Theertha Prasad,Lee, Sang-Yeol,Cho, Moo-Je,Lim, Chae Oh,Choi, Myung-Suk,Bahk, Jeong-Dong,Kim, Myeong-Ok,Hong, Jong-Chan,Yun, Dae-Jin Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-
Bax, a mammalian proapoptotic member of the Bcl-2 family, can induce cell death when expressed in yeast or plant cells. To identify plant Bax inhibitors, we cotransformed a soybean cDNA library and the Bax gene into yeast cells and screened for expressed genes that prevented Bax-induced apoptosis. From the Bax-inhibiting genes isolated, ascorbate peroxidase (aAPX) was selected for characterization. The transcription of aAPX in plants was specifically induced by oxidative stress. Moreover, overexpression of aAPX partially suppressed the H_(2)D_(2)-sensitive phenotype of yeast cytosolic catalase T (Δctt)- and thermosensitive phenotype of cytochrome c peroxidase (Δccp)-deleted mutant cells. Examination of reactive oxygen species (ROS) production using the fluorescence method of dihydrorhodamine 123 oxidation revealed that expression of Bax in yeast cells generated ROS, which was greatly reduced by coexpression with sAPX. Our results collectively suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Bax-induced cell death in yeast.
기(氣)수련이 노인과 청년의 호중구 기능에 미치는 영향
박미숙,이명수,문성록,김용규 圓光大學校 附設 體力科學硏究所 2003 體力科學硏究 Vol.26 No.1
The main objective of this study is to examine the effect of Qi-training on the immune system, especially neutrophil bactericidal function. Nine healthy young male subjects and 9 elderly were studied for the effects of one bout of Qi-training on superoxide (O_(2)) production and adhesion capacity of neutrophils at times immediately after (Post Ⅰ) and 2hours after the Qi-training (Post Ⅱ). The Qi-training enhanced the O_(2) production, reaction velocity and neutrophil adhesion capacity and there were significant differences at Post Ⅰ compared to before Qi-training (Pre) in both group. Therefore, Qi-training increase the resistance of trained individuals against common infection and inflammation.
Bioinspired tuning of glycol chitosan for 3D cell culture
Cho, Myeong Ok,Li, Zhengzheng,Shim, Hye-Eun,Cho, Ik-Sung,Nurunnabi, Md,Park, Honghyun,Lee, Kuen Yong,Moon, Sung-Hwan,Kim, Ki-Suk,Kang, Sun-Woong,Huh, Kang Moo Nature Publishing Group 2016 NPG Asia Materials Vol.8 No.-
<P>Three-dimensional (3D) cell culture systems have promising applications compared with conventional two-dimensional cell culture systems. Herein, we report a facile method for the formation of 3D spheroids using novel thermo-reversible polysaccharide-based hydrogels. A series of thermo-reversible hydrogels consisting of N-acyl glycol chitosans (NAGCs) are synthesized through a simple N-acylation reaction, and the degree of acylation is finely tuned to obtain adequate thermo-reversible properties and gel stability. Among the NAGCs, N-hexanoyl glycol chitosan is the most thermo-sensitive and is highly effective for forming multi-cellular spheroids when used to coat the surfaces of cell culture dishes. Cell spheroids are effectively formed at various cell concentrations, and their spheroid shape and cellular functions are well maintained for longer times. The hydrogel culture system is also useful for co-cultures that mimic a biological microenvironment. Our thermo-reversible hydrogels may offer a convenient method for the development of in vitro 3D cell culture systems to provide enhanced performance in tissue regeneration, organ-on-chips, drug screening research and other biomedical applications.</P>