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RHEE, KI-HYEONG 한국미생물 · 생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.6
The actinomycete strain KH-6 14 possessed strong antifungal activity, especially antagonistic to the rice blast fungus, Pyricularia orvzae. Diaminopimelic acid (DAP) type and morphological and physiological characteristics, examined by scanning electron microscopy (SEM). indicated that KH- 6 14 belonged to the genus Streptornyc'es. Antifungal agent produced by this strain was found to be most active. when the strain was cultured in the presence of glucose, polypeptone, and yeast extract (PY) medium for 6 days at 27℃. Rased on the spectral report data, MS and NMR, the antifungal agent was identified as cyclo(L-leucyl-L-prolyl). According to the antimicrobial activity test measured by minimal inhibitory concentration (MIC), the cyclo(leu-pro) exhibited the activity against Cutzdidu dbiciins 1AM 4905. Mucor rmzunnianus IAM62 18, Rhizoctoriia solani IF0 62 18. Asper~ilii.s,fiimigntu.s ATCC 42202. Glnrnerella citz~ulatn IF0 9767. Trichophtori mentagrophytes ATCC 1 8749. and Trichophytorz nihri'im ATCC 44766. the order of MIC values were 50, 12.5, 5. 50, 25. 5, 5 kg/ml, respectively. Specifically, cyclo(leu-pro) was one of the most effective elements against Pyriculariti oryzae IF0 5994 with the MIC value of 2.5 pg/ml, thus indicating that cyclo(1eu-pro) is a potential antifungal agent.
Protective Effects of Fucoidan against γ-Radiation-induced Damage of Blood Cells
Ki Hyeong Rhee,Keyong Ho Lee 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4
Fucoidan, a sulfated polysaccharide purified from brown algae including Fucus vesiculosus and Laminaria japonica, has a variety of biological activities, including antioxidant and antitumor activities. Here, we investigated the radioprotective effects of fucoidan on human monoblastic leukemia cell line U937. Further, animal tests were carried out using Balb/c mice in order to determine the radiation-induced changes in the counts of blood cells, including thrombocytes, erythrocytes, leukocytes and hematocrit. Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wherein fucoidan (1, 10, and 100 μg/mL) was observed to improve recovery from damage caused by 8-Gy radiation in a dose dependent manner. The viability of U937 cells pre-treated with fucoidan also increased in a dose dependent manner. Furthermore, fucoidan at 100 mg/kg was found to protect against changes in the counts of blood cells as follows: on day 28 after irradiation, the thrombocyte count in the irradiated controls decreased to 45% compared with the non-irradiated controls, while that in the fucoidan-treated group was 60%. The hematocrit in the fucoidan-treated group recovered to 75% on day 28, while that in the irradiated control was 68%. The erythrocyte count in the irradiated controls consistently ranged from 64% to 67% throughout the experiment, but that in the fucoidan-treated group increased gradually, ranging from 75% to 80%. The mean number of survival days and 50-day actuarial survival rate increased dose dependently in the fucoidan-treated group. The mean number of survival days and the 50-day actuarial survival rate in this group was 16, 21, and 29 days and 12%, 20%, and 30% at fucoidan doses of 1, 10, and 100 mg/kg. The values of these parameters in the control group were 9 days and 0%, although the difference between the test and control groups was not statistically significant. Our results may prove valuable in the field of radioprotection.
RHEE, KI-HYEONG 한국미생물 · 생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.6
Cyclo(L-prolyl-L-phenylalanyl) [cyclo(pro-phe)] was isolated from Streptomyces sp. AMLK-335 and found to inhibit DNA topoisomerase Ⅰ activity. In a DNA relaxation assay using supercoiled pBR322 DNA, cyclo(pro-phe) inhibited the DNA topoisomerase activity more strongly than camptothecin, a known topoisomerase inhibitor. However, at a concentration of 10 ㎛, cyclo(pro-phe) produced a lower degree of DNA relaxation than camptothecin, therefore, the inhibition topoisomerase Ⅰ activity by cyclo(pro-phe) was also found to be dose dependent. Accordingly, the current results suggest that cyclo(pro-phe) may be a novel inhibitor of topoisomerase Ⅰ.
다제내성 Acinetobacter baumannii의 생장을 억제하는 항생물질을 생산하는 방선균의 분리,동정 및 항균효과
이기형 ( Ki Hyeong Rhee ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.1
계룡산 지역에서 토양시료를 채취하여 항생제 다제내성균을 효과적으로 제어할 수 있는 항생물질 생산균주를 분리, 탐색하는 과정에서 Streptomyces sp. KH223균주를 선별하였다. 분리균주의 생화학적 특징과 16S ribosomal DNA 유전자 염기서열 분석 결과, 분리균주가 Streptomyces galbus에 속함이 확인되었다. 분리균주의 배양 상등액은 49종의 Acinetobacter baumannii 외 다양한 세균과 진균 등에 항균활성을 가지고 있었으며 특히 분리균주의 부탄올, 에틸아세테이트 분획물은 10여종의 A. baumannii에 최소생육저해농도 범위 0.8 μg/mL에서 5.0 μg/mL 으로 강한 항균력을 보였으며 기 분리된 cyclo(trp-trp)의 최소생육저해농도 범위 12.5 μg/mL 보다도 강한 항균활성을 나타내었다. 또한 각각의 유기용매 분획물과 cyclo(trp-trp)은 Candida albicans IFO 6258, Bacillus subtilis IAM 1069, Micrococcus luteus JCM 1464, Aspergillus niger ATCC 9642, Streptomyces murinus JCM 4333 등 5종의 세균과 진균에 대해 2.5 μg/mL에서 50μg/mL 범위의 비교적 강한 항균력을 나타내었다. 본 실험을 통하여 얻은 결과는 분리균주의 유용성과 앞으로 추가적인 정제, 물질동정 실험을 통하여 새로운 항생물질을 탐색하는 기초를 제공할 수 있을 것으로 판단된다. I isolated the actinomycete strain KH223 from soil samples collected from the Kye Ryong mountain area. This strain is antagonistic to the multidrug-resistant Acinetobacter baumannii. KH223 was confirmed as belonging to the genus Streptomyces based on the scanning electronmicroscopy(SEM) observations of the diaminopimelicacid(DAP) type and morphological and physiological characteristics. Comparison of the 16S rDNA nucleotide sequences revealed that KH223 has a relationship with Streptomyces galbus. Production of antibiotics by KH223 was most favorable when cultured on a glucose, polypeptone, and yeast extract(PY) medium for 6 days at 27oC. The supernatant was found to exhibit an antimicrobial effect on various kinds of bacteria and fungi. Particularly, butanol and ethylacetate extracts of KH223 and cyclo(trp-trp) exhibited significant activity against A. baumannii at concentration ranges of 0.8-12.5 μg/mL, 5.0-25 μg/mL and 12.5→100 μg/mL, respectively. Moreover, in contrast to cyclo(trp-trp) had shown to activity against Micrococcus luteus JCM 1464 at the concentration of 12.5 μg/mL, the butanol extract of KH223 showed significant activity against Bacillus subtilis IAM 1069 and Micrococcus luteus JCM 1464 at the concentration of 0.4 and 0.8 μg/mL, respectively. These results suggest that KH223 may have a great potential in the production of new antibiotics to combat multidrug-resistant pathogens and further studies may be warranted for the same.
두개강내로 침범한 두개골막에서 발생한 악성 섬유성조직구종 : 증례보고 A Case Report
박형근,이상평,최기환,박재복,여행태,이정길 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.3
최근 본원에서 신경외과 영역에서는 비교적 치험하기 힘든 종양인 MFH가 두개골막에서 발병하여 두개골 및 뇌경막을 침범하여 두개강내로 파급된 예를 치험하고 문헌 고찰과 함께 보고하였다. A case of malignant fibrous histiocytoma(MFH) arising from the pericranium which extend to the calvaria and the dura mater in a 32-year-old man is reported. This tumor presented as scalp induration and headache without any other neurologic abnormalities. Radical excision presented a surgical challenge because of the extensiveness of the lesion. In this %case radical excision of pericranial and calvarial lesions were possible, however it is impossible to remove the lesion in the dura mater radically because of its extensiveness. Thus the radiation therapy was necessary after operation. Histologically, pericranial, calvarial and dura mater tumor tissues were same as that of the typical malignant fibrous histiocytoma of the soft tissue. The authors review the literatures of the MFH involving central nerve systems and discuss the management and the radiologic findings of this rare tumor.