Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells

Qian, Jun,Li, Jing,Jia, Jian-Guang,Jin, Xin,Yu, Da-Jun,Guo, Chen-Xu,Xie, Bo,Qian, Li-Yu Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Objectives: To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells. Materials and Methods: SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting. Results: It was found that the proliferation of SP was significantly faster than that of NSP (P<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (P<0.05). Conclusions: With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.

Net micromineral requirements for maintenance and growth of ewe lambs at the latter fattening period

Jin Ya Qian,Ding Na,Diao Xiao Gao,Yu Sheng Chen,Zhao Jun Xing,Zhang Jian Xin 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.9

Objective: The objective of this study was to investigate the net micromineral (Cu, Fe, Mn, Zn) requirements for maintenance and growth of Dorper×Jinzhong crossbred ewe lambs at their latter fattening period. Methods: Thirty 1/2 Dorper × 1/2 Jinzhong crossed F1 ewe lambs (35±0.5 kg of body weight [BW]) were used and divided into five groups in a randomized design for a comparative slaughter trial. At the beginning of the experiment, six lambs were randomly selected and slaughtered at 35 kg BW to determine their initial body composition. When their BWs reached 43 kg, another six lambs fed ad libitum were slaughtered to serve as an intermediate slaughter group. The retained eighteen lambs were randomly distributed into three groups and offered one type of feed at 100%, 65%, and 40% of the ad libitum intake. When the lambs fed ad libitum reached a BW of 50 kg, the three groups were slaughtered. The body composition (muscle, fat, bone, blood with viscera, skin, and wool) were weighted, ground, mixed, and subsampled for mineral content analysis. Results: The net maintenance requirements of Cu, Fe, Mn, and Zn were 0.017, 0.160, 0.004, and 0.067 mg/kg BW/d, respectively, and the net growth requirements per 100 grams of average daily gain ranged from 0.48 to 0.51 mg of Cu, 2.63 to 2.17 mg of Fe, 0.12 to 0.15 mg of Mn, and 2.07 to 2.00 mg of Zn, respectively, for Dorper × Jinzhong crossed ewes from 35 to 50 kg BW. Conclusion: Our results suggest that the micromineral requirements for both maintenance and growth of Dorper × Jinzhong crossbred ewe lambs were quite different from the recommendations of NRC (2007), except for Zn.

Structure and Properties of Low Dielectric Constant Polyetherimide Films Containing-CF3 and Cardo Groups

Jun Peng,Qian Wang,Jin Wang,Jun Yang,Taijun Jiang,Guangsheng Zeng 한국고분자학회 2022 Macromolecular Research Vol.30 No.11

Cardo containing phenoxy and -CF3 derivate diamine was synthesized, and further it was polymerized with 3,3',4,4'-biphenyl tetracarboxylic dianhydride to prepare polyetherimide containing Cardo group. The transmittance of the prepared polymer increased from 82% to 88% and the dielectric constant decreased from 2.91 to 2.83 with a slight decline in thermal and mechanical performance when -CF3 was introduced. The molecular simulation in terms of charge distribution, electric potential energy, chain stiffness and aggregation state revealed that -CF3 could restrain charge transfer and decrease the unit dipole moment and polarization for its larger volume. The optical and dielectric performance was significantly enhanced, but the thermal and mechanical performance decreased slightly when the -CF3 group was introduced. The glass transition temperature of the polymer declined from 329℃ to 311℃. The investigation conducted in this paper can offer valuable reference to the optimization and development of synthesis and comprehensive performance of polyimide.

Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus)

Mei Jin,Qian Cao,Ruilong Wang,Jun Piao,Fengqin Zhao,Jingai Piao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.3

Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness. Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization. Results: Bioinformatics analysis showed that KAP11.1 gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that KAP11.1 gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of KAP11.1 has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, KAP11.1 has a significantly higher expression in anagen than in catagen. Moreover, KAP11.1 gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb. Conclusion: We conclude that KAP11.1 gene may play an important role in regulating the fiber diameter.

Protectin DX increases alveolar fluid clearance in rats with lipopolysaccharideinduced acute lung injury

Xiao-Jun Zhuo,Yu Hao,Fei Cao,Song-Fan Yan,Hui Li,Qian Wang,Bi-Huan Cheng,Bin-Yu Ying,Bin-Yu Ying,Sheng-Wei Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Acute respiratory distress syndrome is a life-threatening critical syndrome resulting largely from the accumulation of and the inability to clear pulmonary edema. Protectin DX, an endogenously produced lipid mediator, is believed to exert anti-inflammatory and pro-resolution effects. Protectin DX (5 μg/kg) was injected i.v. 8 h after LPS (14 mg/kg) administration, and alveolar fluid clearance was measured in live rats (n = 8). In primary rat ATII epithelial cells, protectin DX (3.605 × 10−3 mg/l) was added to the culture medium with LPS for 6 h. Protectin DX improved alveolar fluid clearance (9.65 ± 1.60 vs. 15.85 ± 1.49, p < 0.0001) and decreased pulmonary edema and lung injury in LPSinduced lung injury in rats. Protectin DX markedly regulated alveolar fluid clearance by upregulating sodium channel and Na, K-ATPase protein expression levels in vivo and in vitro. Protectin DX also increased the activity of Na, K-ATPase and upregulated P-Akt via inhibiting Nedd4–2 in vivo. In addition, protectin DX enhanced the subcellular distribution of sodium channels and Na, K-ATPase, which were specifically localized to the apical and basal membranes of primary rat ATII cells. Furthermore, BOC-2, Rp-cAMP, and LY294002 blocked the increased alveolar fluid clearance in response to protectin DX. Protectin DX stimulates alveolar fluid clearance through a mechanism partly dependent on alveolar epithelial sodium channel and Na, K-ATPase activation via the ALX/PI3K/Nedd4–2 signaling pathway.

<i>De novo</i> genetic variation associated with retrotransposon activation, genomic rearrangements and trait variation in a recombinant inbred line population of <i>Brassica napus</i> derived from interspecific hybridization with <i>Brassica rapa</i>

Zou, Jun,Fu, Donghui,Gong, Huihui,Qian, Wei,Xia, Wei,Pires, J. Chris,Li, RuiYuan,Long, Yan,Mason, Annaliese S.,Yang, Tae‐,Jin,Lim, Yong P.,Park, Beom S.,Meng, Jinling Blackwell Publishing Ltd 2011 The Plant journal Vol.68 No.2

<P><B>Summary</B></P><P>Interspecific hybridization is a significant evolutionary force as well as a powerful method for crop breeding. Partial substitution of the AA subgenome in <I>Brassica napus</I> (A<SUP>n</SUP>A<SUP>n</SUP>C<SUP>n</SUP>C<SUP>n</SUP>) with the <I>Brassica rapa</I> (A<SUP>r</SUP>A<SUP>r</SUP>) genome by two rounds of interspecific hybridization resulted in a new introgressed type of <I>B.?napus</I> (A<SUP>r</SUP>A<SUP>r</SUP>C<SUP>n</SUP>C<SUP>n</SUP>). In this study, we construct a population of recombinant inbred lines of the new introgressed type of <I>B.?napus</I>. Microsatellite, intron‐based and retrotransposon markers were used to characterize this experimental population with genetic mapping, genetic map comparison and specific marker cloning analysis. Yield‐related traits were also recorded for identification of quantitative trait loci (QTLs). A remarkable range of novel genomic alterations was observed in the population, including simple sequence repeat (SSR) mutations, chromosomal rearrangements and retrotransposon activations. Most of these changes occurred immediately after interspecific hybridization, in the early stages of genome stabilization and derivation of experimental lines. These novel genomic alterations affected yield‐related traits in the introgressed <I>B.?napus</I> to an even greater extent than the alleles alone that were introgressed from the A<SUP>r</SUP> subgenome of <I>B.?rapa</I>, suggesting that genomic changes induced by interspecific hybridization are highly significant in both genome evolution and crop improvement.</P>

Dynamic Frequency of Blood CD4+CD25+ Regulatory T Cells in Rats with Collagen-induced Arthritis

Tingyu Wang,Jun Li,Zhigui Jin,Feihua Wu,Yunwu Li,Xiaohua Wang,Hong Zhou,Qian Zhou 대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.2

CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs) have been shown to play a regulatory orsuppressive role in the immune response and are possibly relevant to the pathogenesis of autoimmunediseases. In the present study, we attempted to investigate the frequency of CD4+CD25+ Tregs inperipheral blood (PB) of collagen-induced arthritis (CIA) rats during the development of arthritis, todetermine whether their frequency is involved in the immunoregulation of this disease. The resultsshowed that normal rats had similar frequencies of CD4+CD25+ Tregs in PB during the experimenttime, expressed as a percentage of CD4+CD25+Foxp3+ T cells among the CD4+ T lymphocyte population. In contrast, the frequency of CD4+CD25+Foxp3+ T cells in CIA rats was found to change duringthe development of arthritis. In CIA rats, there is a significant negative correlation betweenthe frequency of CD4+CD25+Foxp3+ T cells and paw swelling (r=−0.786, p< 0.01). The relationshipbetween the frequency of CD4+CD25+Foxp3+ T and immune activation was not found in normal rats. During the time course, the frequency of CD4+CD25+Foxp3+ T was lower in CIA rats than in normalones. The data suggest that the frequency of PB CD4+CD25+ Tregs may be a promising marker forarthritis activity.

Preparation and Properties of Green Environment-Friendly Drilling Polymer Mud

Zhang, Feng-Jun,Sun, Xian-Yang,Li, Xuan,Kong, Cui,Liu, Jin,Chen, Qian-Bao,Oh, Won-Chun Materials Research Society of Korea 2019 한국재료학회지 Vol.29 No.11

In this paper, a water-based green polymer mud is synthesized by simple compounding method. Effects of different kinds of tackifiers, their molecular weight on the viscosity of polymer mud and the effects of different fluid loss additives on mud fluid loss are studied. The results show that when polystyrene and anionic polyacrylamide with molecular weight of 8 ~ 10 million are used as the main thickening ingredient, polymer mud with high viscosity and high stability can be obtained. When the prepared polymer mud is formulated as NPAM: PEO: Hydroxypropyl cellulose(HPC) : Water = 42:10:10:100000 (unit: kg), the viscosity can reach 20.6 s, the filtration loss in 7.5 min is 24 mL, and the sand content is only 0.1 %. Compared with traditional bentonite mud, the green environment-friendly polymer mud has the advantages of small amount of waste, low environmental pollution, and low pulping cost, and can meet the construction needs for most topography and geomorphology drilling engineering.

Halomonas alkalitolerans sp. nov., a Novel Moderately Halophilic Bacterium Isolated from Soda Meadow Saline Soil in Daqing, China

Shuang Wang,Qian Yang,Zhi-Hua Liu,Lei Sun,Dan Wei,Jun-Zheng Zhang,Jin-Zhu Song,Yun Wang,Jia Song,Jin-Xia Fan,Xian-Xin Meng,Wei Zhang 한국미생물학회 2011 The journal of microbiology Vol.49 No.1

A moderately halophilic bacterial strain 15-13^T, which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13^T were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13^T were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C18:1 ω7c (60.48%) and C16:0 (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13^T clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13^T was Halomonas pantelleriensis DSM 9661^T (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H. pantelleriensis DSM 9661^T was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13^T represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13^T (=CGMCC 1.9129^T =NBRC 106539^T).

Dynamic analysis of Ca^(2+) level during bovine oocytes maturation and early embryonic development

Su Li Liang,Qian Jun Zhao,Xiang Chen Li,Ya Ping Jin,Yi Peng Wang,Xiao Hua Su,Wei Jun Guan,Yue Hui Ma 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.2

Mammalian oocyte maturation and early embryo development processes are Ca^(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca^(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca^(2+) was distributed in the cortical ooplasm and throughout the oocytes from the MI to MII stage. In IVF embryos, Ca^(2+)was distributed in the cortical ooplasm before the formation of the pronucleus. In 4-8 cell embryos and morulas, Ca^(2+) was present throughout the blastomere. In PA embryos, Ca^(2+) was distributed throughout the blastomere at 48 h, similar to in the 4-cell and 8-cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca^(2+) in the SCNT embryos. However, Ca^(2+) was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca^(2+) showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca^(2+) location and distribution changes during oocyte maturation and early embryo development processes should facilitate a better understanding of the mechanisms involved in oocyte maturation, reconstructed embryo activation and development, ultimately improving the reconstructed embryo development rate.