연장 가골에 골 이식 대체물 투여가 골 경화에 미치는 영향 : 가토 경골에서의 방사선 및 골 밀도의 변화 Changes of Radiography & Bone Mineral Density in the Tibia of Rabbits

오창욱,김풍택,박병철,송해룡,박일형,백준호,박형진 경북대학교 병원 2003 경북대학교병원의학연구소논문집 Vol.7 No.1

목적 : 골 연장술 후 생기는 연장 가골에 황화 칼슘과 이종 이식골을 투여하여 골 경화 속도에 대한 영향을 알아보기 위한 실험이었다. 연구대상 및 방법 : 성장중인 뉴질랜드 화이트 토끼(2.0-2.5㎏)의 경골 간부에 골막을 보호하고, 절골술을 시행하고, 5일간의 휴지기후 외고정 장치를 이용하여 1주일 동안 7㎜가 연장될 때까지 하루 1㎜씩 2회의 리듬을 가지고 골 연장을 실시하였다. 제1 실험 군 7마리에는 연장이 끝난 직후 황화 칼슘(Osteoset^(ⓡ), Wright medical USA) 1정, 제 2 실험 군 7마리에는 이종 이식골의 일종인 Lubboc 5㎟을 각각 연장된 가골 부에 주입하고, 단순 연장한 대조군 7마리와 함께, 방사선 및 골 밀도비를 측정하고 추시하여, 골 경화의 진행속도를 비교하였다. 방사선 촬영은 전후면 및 측면사진을 1주 간격으로 촬영하였고, 골밀도 측정은 DEXA(dual energy X-ray absorptionmetry, Lunar^(ⓡ)) 장치를 2주일 간격으로 시행하였다. 결과 : 방사선 소견상, 제 1실험군의 연장가골의 완전 경화시간은 평균 14주, 제 2실험군은 154 주로 각각 대조군의 16.9주보다 단축되었으며, 골 밀도 비에서도, 두 실험군에서 최고치가 대조군의 값보다 높고, 최고치까지의 경과기간도 빨랐다. 결론 : 본 연구에서 연장 가골에 황화칼슘 및 이종 이식골의 투여가 골 경화의 기간을 단축시킬 수 있고, 외고정 장치의 빠른 제거에 도움이 될 수 있음을 알았다. Purpose : This study was designed to know the effect of calcium-sulfate and xenograft on the distracted callus after lengthening. Materials & Methods : We had operation of subperiosteal osteotomy and external fixation on the tibial diaphysis of young New Zealand White rabbits(2.0-2.5㎏); after 5 days of latency period, 7㎜(1㎜/day, 2times/day) of tibial lengthening was reached in a week. At 1 week after lengthening, the 1st experimental group of 7 rabbits received a pellet of calcium sulfate(Osteoset, Wright medical, USA) in the distraction gap, and the 2nd experimental group of 7 rabbits received 5㎜ 2 of xenogrfat(Lubboc) in the distraction gap. But, the control group of 7 rabbits did not receive any of above materials. We compared three groups with the changes of radiographic findings at every week and bone mineral ratio(DEXA) at every two weeks. Results : The time to complete consolidation of distraction callus of both experimental group(calcium sulfate;14 weeks, xenograft; 15.4 weeks) was shorter than that of control group(16.9 weeks) in radiographic findings. Maximum value of bone mineral ratio of distraction callus was higher and the time to reach the highest value was also shortened in the both experimental group compared to control group. Conclusion : By use of bone substitutes as like calcium sulfate or xenograft in the distraction callus with external fixator, it may be possible to shorten the consolidation period and the fixator-wearing period.

축전용량 측정법에 의한 ZnO-O₂경계면에 대한 연구

오형식,전동철 木浦大學校基礎科學硏究所 1988 基礎科學硏究誌 Vol.6 No.-

반도체와 기체 사이에서의 거동을 조사 하는데 축전 용량 측정법이 전도도 측정을 대신할 수 있게 되었다. 산화아연 ?? 에 산소가 흡착 될때 형성되는 공간 전하 영역의 길이 ??를 축전용량 법을 이용하여 조사 하였고 흡착 과정에서 고갈층이 형성 됨을 알 수 있었다. 시료를 773K에서 1시간 배기 시킨후 흡착 온도(298K∼673K)에 따른 ??의 변화를 조사한 결과 온도가 증가할 수록 ??가 증가(112Å∼331Å)함을 알 수 있었다. 이러한 결과들로 부터 산소는 산화아연 표면에 받게 형태로 흡착되고 흡착 과정에서 표면에 에너지 장벽이 형성 됨을 알 수 있었다. Capacitance measurement was presented for studing semiconductor-gas interface. The depth of the space charge region ??, which has developed in the chemisorption of oxygen on ZnO single crystal, has been estimated from the capacitance measurements. After the sample was evacuated at 773K for 1 hr, the value of ?? appeared largely when adsorbed temperature was increased. From the results, it was suggested that oxgen was adsorbed as an acceptor on ZnO single crystal and an energy barrier at the surface was formed during the adsorption process.

유동층을 이용한 탈황제의 마모특성에 관한 연구

오광중,김형국,최은화,조기철 부산대학교 환경문제연구소 1999 環境硏究報 Vol.17 No.1

For the efficient energy use and the protection of environmental pollution, attrition characteristics, depending on the component and the condition of sorbent for the purification of fuel gas, were analyzed by a fluidized-bed tester. Results of these studies can be summarized follows. 1) When Mn-based sorbent(M, MT, MFT) prepared with varying addictives and induration conditions was compared in terms of particle size distribution and AI(Attrition Index), MT and MFT possessing TiO2 and Fe203 had much higher attrition resistance than M. Attrition resistance of M, MT and MFT increased as bentonite contents and induration temperature increased, when bentonite contents induration temperature were changed from 2 to 5% and from 1000 to 1100℃, respectively. Therefore when powdery catalysts are prepared, the attrition rate can be considerably affected by the controls of addictive, binder and induration temperature. 2) Attrition tests using MT1100-5 were performed under the conditions that particle size distribution was uniform and had much more over +270mesh and under -325mesh. AI due to attrition was 8.83, 21.54 and 1.67%, respectively. Thus, initial particle size distribution showed a great influence on both produced fines distribution and attrition loss. Therefore, the size distribution of particles injected initially and the particle size that carried over showed be considered to reduce the loss of particulate materials and the replacement cost due to attrition. 3) An experiment using MT1100-5 sorbent was also carried out to predict AI as a function of time and particle size distribution during 23hr. AI of prepared sorbent was 4.54, 7.73, 9.08, 11.35 and 12.48 after 1.5, 3, 5, 9 and 23hr. respectively. Thus, it was shown that the amount of produced fines during the same time was shorten with the increase of time. The operating condition of dust capturing equipment and the rate of powder exchange needs to be considered because most of the fines due to fluidization of particulate material was generated at initial operation.

아세톤-물 혼합용매내에서 1_1 전해질의 전기전도도에 대한 연구

오형식,김종현,전동철 木浦大學校基礎科學硏究所 1988 基礎科學硏究誌 Vol.6 No.-

여러가지 조성의 아세톤-물 혼합용매중에서 LiC1, LiBr 및 Lil의 희박용액 전도도를 25℃에서 측정하였다. 그 결과 각 전해질들의 여러 농도에 따르는 당량전도도의 변화가 Fuoss-Onsager's semiem-pirical 식에 대해서 좋은 일치를 보여 주었다. 또한 아세톤-물 혼합용매의 몰 분율이 증가함에 따라 극한당량전도도??값이 LiC1, LiI는 0.3 몰 분율에서, LiBr는 0.2 몰 분율에서 가장 작은 값을 나타내었다. Walden Product??는 LiC1와 LiBr가 0.1 몰 분율에서, LiI는 0.0 몰 분율에서 최대치를 보여 주었고, 그 후로는 아세톤 몰 분율의 증가에 따라 계속 감소하였다. The equivalent conductance of LiC1, LiBr and LiI have been measured in Acetone-Water mixtures of various composition (0.0∼0.6 mole fraction) at 25℃. The value of equivalent conductance agreed well with the Fuoss-Onsager's semiempirical equation. The minimum limiting equivalent conductance was found in 0.3 mole fraction Acetone for LiC1, LiI and LiBr was found in 0.2 mole fraction Acetone. The maximum Limiting Walden Products?? of LiC1 and LiBr was found in 0.0 mole fraction Acetone, and LiI was found in 0.0 mole fraction Acetone. And also, after 0.1 mole fraction and 0.0 mole fraction, decreased with increasing of mole fraction Acetone.

제주 우수 육상경기 선수들의 년중 훈련효과 분석 및 처방

김철원,임상용,김승곤,오만원,김성찬,이창준,남사웅,이세형,류재청 濟州大學敎 體育科學硏究所 1996 체육과학연구 Vol.2 No.-

The study was undertaken to analysis, evaluate, and prescribe t h e annual effect of training of periodically anaerobic and aerobic motor ability for Che-Ju representive team of elementary, middle and high school athletes. first. the performance can be obtained when it was prescribed proper training after analysis of physical fitness by each events. second, individually fitness. scientific analysis. prescription. proper management of athlete will be necessary to apply. In addition to physiology aspect, it is essential to consider sport biomechanics. sport psychology when we prescribe specific events and necessary approach in demension coach-athlete-researcher for individual.

제주 우수 수영 선수들의 년중 훈련효과 분석 및 처방

김철원,임상용,김승곤,오만원,김성찬,이창준,남사웅,이세형,류재청 濟州大學敎 體育科學硏究所 1996 체육과학연구 Vol.2 No.-

The study was undertaken to analysis, evaluate, and prescribe the annual effect of training of periodically anaerobic and erobic motor ability for Che-Ju representive team of elementary, middle and high school athletes, first the performance can be obtained when it was prescribed proper training after analysis of physical fitness by each events, second individually fitness scientific analysis. prescription. proper management of athlete will be necessary to apply.

도석중의 불순물제거에 관한 연구

한오형,마동철 조선대학교 동력자원연구소 1995 動力資源硏究所誌 Vol.17 No.1

In order to remove impurities which are pyrite particles mainly contained in pottery stone, mineralogical characteristics, particle size, pH, flotation time and the effection of collector amounts was examined. The raw minerals were analyzed by microscopy and X-ray diffraction, and the products of the flotation were analyzed by atomic absorption spectroscopy. The results obtained from these experiments are as follows; 1. Pottery stone mainly consists of quartz, montmorillonite, and pyrite. 2. Liberation effect was increased in smaller than large particle size because of ratio of surface area increasing. Suitable condition of seperated particle size was 60×100mesh. 3. The depressing effect of pottery stone was increased in acid region, but flotation effect of pyrite was much in effect at pH7. 4. Flotation rate of pyrite was over 97% at 5 min., and flotation rate was not changed over 5min.. Thus, the optimum flotation time was less than 5 min.. 5. The 96.15 percents of pyrite could be recovered with yield of pottery stone of about 90 percents by using 200g/t of KAX. Therefore, the optimum concentration of collector was appeared to be about 200g/t.

다환방향족 탄화수소에 오염된 토양에서 분리한 Pseudomonas sp. KK1의 생리학적 특성

윤병준,강형일,오덕철 제주대학교 생명과학연구소 2001 제주생명과학연구 Vol.4 No.-

Strain KK1 was isolated from soil contaminated with polycyclic aromatic hydrocarbons and able to mineralize anthracene, naphthalene, and phenanthrene. In this study, strain KK1 was tested to evaluate carbazole-degrading potential using radiorespirometry. When KK1 was pre-grown on phenanthrene the cells were able to mineralize carbazole much more rapidly, suggesting a possible close linkage between the pathways for catabolism of carbazole and phenanthrene. Such an increase in carbazole degradation was not appreciable when KK1 was pre-grown on naphthalene. Strain KK1 was identified as the genus Pseudomonas with over 90 % confidence based on BIOLOG system and FAME analysis. Analysis of PLFAs extracted from kk1 cells on carbazole medium revealed that lipids 10:0 3OH, 17:0 cyclo, and 18:0 were representatives produced or significantly increased in response to carbazole. Tests for antibiotics provided the data that strain KK1 was resistant to antibiotic ampicillin, but susceptibile to chloramphenicol, gentamycin, kanamycin, streptomycin, and tetracycline. Strain KK1 demonstrated strong resistance to most heavy metals such as Ba, Cd, Fe, Hg, Pb used in this study.

심첨부 비후 심근증에서 심근 내 미세 관상동맥의 혈류 예비력 평가 : 경흉부 도플러 심초음파를 이용한 연구 Study Using Transthoracic Doppler Echocardiography

윤호중,박철수,신재원,임상현,조은주,정해억,전희경,오용석,정욱성,김재형,최규보,홍순조 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.3

광합성 세균 Rhodospirillum rubrum S1이 생성한 Catalase-Peroxidase의 부분 정제 및 특성 규명

김영미,이동헌,강형일,오덕철 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

호기적으로 배양한 광합성 세균 Rhodospirillum rubrum SI은 5 가지의 catalase를 생성함을 알 수 있었다. 그 중 peroxidase의 기능도 동시에 갖는 catalase-peroxidase(Cat-3)를 부분 정제하고 그 특성을 조사하였다. 부분정제된 catalase-peroxidase의 수율은 catalase가 1.6% 그리고 peroxidasse가 5.1% 였으며, 정제 배수가 4.6배와 14배로 증가한 효소를 얻을 수 있었는데, catalase보다 peroxidase의 정제배수가 더 높았다. pH에 대한 영향은 catalase활성은 pH6에서, peroxidase활성은 pH5에서 가장 높게 나타났다. 온도에 대한 영향은 catalase와 peroxidase 활성이 모두 30℃에서 최고의 활성을 보이는 것으로 나타났으며, 온도에 대한 효소의 안정성은 50℃에서 catalase가 peroxidase보다 더 안정함을 알 수 있었다. 부분정제된 catalase-peroxidase에 유기용매와 10mM 3-amino-1,2,4-triazole을 처리한 결과, 유기용매에 대해 catalase의 활성은 79%, peroxidase의 활성은 85%까지 억제됨을 알 수 있었으며, 10mM 3-amino-1,2,4-triazole에 대한 각 효소의 활성은 그대로 유지되고 있었다. 전형적인 heme단백질 효소들의 저해제로 알려진 NaCN, NaN3, NH20H를 농도별로 처리한 결과, NaCN인 경우 catalase는 8.72×10-s M의 농도에서, peroxidase는 5.1×10-s M의 농도에서, NaN3에서 catalase는 4.2×10-7 M, peroxidase는 3.2×10-7 M, NH20H에서는 catalase인 경우는 2.0×10-7 M, peroxidase인 경우는 2.5×10-7M 농도에서 억제되었으며, 이들 저해제들은 catalase활성과 peroxidase활성을 동시에 저해하는것으로 나타났다. Five different catalases have been already found in the aerobically grown photosynthetic bacterium Rhodospirillum rubrum S1. Among them, a bifunctional catalase-peroxidase, designated Cat-3 was partially purified and characterized. The enzyme was purified through four steps in 1.6% yield for catalatic activity and 5.1% yield for peroxidatic activity. On the basis of catalatic activity, the protein purification increased nearly 4.6-fold, whereas for peroxidatic activity, an approximately 14-fold purification was achieved. The optimum pHs of the catalatic and peroxidatic activities were 6 and 5, respectively, and the optimum temperature for both activities was 30℃. The catalatic activity of the enzyme maintained at 50℃ for lh was more stably than peroxidatic activity. The catalatic and peroxidatic activities were inhibited about 79 % and 85 % by exposure to organic solvent(ethanol/chloroform), respectively. Both enzymatic activities were not neally inhibited by lOmM 3-amino-1,2,4-triazole. Fifty percent enzyme inhibition of the catalatic activity was reached with 2.0×10^(-7)M, hydroxylamine, 4.2×10^(-7)M azide and 8.7×10^(-6)M cyanide, and that of the peroxidatic activity was obtained with 2.5×10^(-7)M hydroxylamine, 3.2×10^(-7)M azide and 5.1×10^(-6)M cyanide.