Fusobacterium nucleatum의 테트라사이클린 내성여부를 검사하기 위한 세균배양법과 중합효소연쇄반응법의 비교

최재흥,성진효,국중기,김동기 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.4

The purposes of this study were to identify the detection and incidence of tetracycline resistance determinant tet(M) by PCR method and to evaluate the availability of PCR method guided for clinical use of antibiotics in dentistry. Subgingival plaque samples were collected from 172 sites of 29 patients. Both original sample and subculturd sample, PCR amplification was used to detect tet(M) in Fusobacterium nucleatum. The percentage of tet(M)-positive subjects and sites in original samples were 100%, 95.9%, respectively. And the percentage of tet(M)-positive subjects and sites in subcultured samples were 17.2%, 3.5%, respectively. In subcultured samples, probing pocket depth and clinical attachment level in tet(M)-positive sites were higher than in negative sites (p<0.05). The sensitivity and specificity of PCR method compared with broth dilution method as a gold standard were 0.91 and 0.86, respectively. The results suggested that tet(M) assay using PCR method was available to detect the tetracycline-resistant F. nucleatum.

그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력

최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1

An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.

Flavobacterium meningosepticum으로부터 카로테노이드 색소의 생산을 위한 최적 배양조건

진효상,최영순 전주대학교 자연과학종합연구소 2003 전주대학교 자연과학연구소 학술논문집 Vol.15 No.1

A carotenoid producing bacterium was selected from soil and identified as Flavobacterium meningosepticum. An optimum condition was seeked to produce the carotenoids from the selected bacterium. The carotenoid production was best when the bacterium was cultivated at 35 ℃ for 36 hour in the medium composed of 1.0% of tryptone and 0.1% each of MgSO_(4)·7H_(2)HPO_(4), and NaCl(pH 6.0) with shaked at 200 rpm on the 1:l0 volume ratio of medium to flask. The yield was 660 ㎎/ℓ at the above condition.

1~2기 본태성 고혈압 환자들에서 Imidapril 대 Enalapril의 항고혈압 효과를 평가하기 위한 무작위, 이중맹검 임상시험

최영진,손대원,김용진,이홍자,채인호,김효수,김철호,오병희,이명묵,박영배,채윤식,이영우 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

수술중 수혈환자의 임상적 고찰 (Ⅱ)

최세진,신효철 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

Over the past several decades, blood and blood components have been increasingly recognized as medication rather than innocuous fluids used to replace modest blood lose at operation. But blood transfusions have the potential for many harmful side effects. Therefore, modern hemotherapy practice focuses on the benefits and risks, along with profer dosage, items with which blood component transfusion is concerned, when blgod transfusions are indicated. only that fraction of the blood that the patient requires should be administered. Most blood donations are separated into components after collection : red blood cells, platelet concentrates, and/or cryoprecipitates and plasma. No longer is "fresh whole blood" an appropriate response to a hemotherapeutic problem. This study was undertaken to evaluate transfusion according to surgical department, sex, preoperative hemoglobin values, blood type, transfusion amounts and blood component therapy. 3847 transfused patients among the total 21371 surgical cases done at the Chung-Nam National University Hospital from 1985 to 1989 were reviewed. The results are summerized as follow : 1. The numbers of transfused patients among the 21371 operative cases were 3847 cases (18%). 2. The number of male transfused was greater than that of female and third decade patient was the greatest incidence among the transfused patients. 3. Mostly hemoglobin value in preoperative state was maintained more than 10g/dl. 4. The blood types of transfused blood were in the great order of A. B, O, AB type. 5. The percentage of one pint transfusion was gradualy decreased and 2-5 pint transfusion was greatest percentage namely 57.94% 6. As the amount of blood transfused was increased, the percentage of blood compoent therapy was increased, and mixed case of PRC and FFP was the greatest incidence. 7. The greatest percentage of blood component therapy was in the cases of the orthopedic surgical operation.

雜犬에서 Isoflurane 마취시 Epinephrine 용량에 의해 유발된 부정맥의 비교연구

최세진,최광희,신효철 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

Isoflurane has been known to reveal relatively stable hemodynamic effects on cardiovascular system. So many anesthesiologist recommand it as the anesthetic choice for cardiovascular surgery. Epinephrine injection for surgical hemostasis during general anesthesia especially exogenous epinephrine injection with halothane & cyclopropane induced the arrhythmias. The author performed the experimental study to evaluate the arrhythmias and cardiovascular effects during 1% isoflurane anesthesia, when diluted solution of epinephrine intravenous injected. Systolic Blood Pressure, Diastolic Blood Pressure, Mean Arterial Pressure, Heart Rate, and presence of arrhythmia were measured. 1. Arrhythmias were few and transient in diluted epinephrine concentration increase. 2. SBP, DBP, MAP were increased in diluted epinephrine concentration increase. 3. Heart rate were decrease at systolic pressure peak level.

영구형 항서맥 심박조율기 환자의 장기추적관찰

최현석,이무용,최영진,최성준,김효수,손대원,오병희,이명묵,박영배,최윤식,이영우,이명용 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

Flavobacterium meningosepticum이 생산하는 Carotenoids의 동정 및 생리활성

진효상,최영순 전주대학교 자연과학종합연구소 2003 전주대학교 자연과학연구소 학술논문집 Vol.15 No.1

Some carotenoids isolated from F. meningosepticum showed 3 different bands on thin layer chromatogram, one of which was the dominant. The dominant band had 455 nm of λmax, showed negative result on epoxide test, 20 nm of basochromic shift on ketone test, and absorption band characteristic of hydroxyl group on IR spectrum, which means that the main component is hydroxyechinenone. Also, the carotenoids had ROS scavenging activity and antioxidant activity. The carotenoids showed no cytotoxic activity. So it was found out that this carotenoids could be used in industry.

석탄회 펠렛의 미연탄소 연소 현상 분석

崔尙丁,李孝眞,吳明淑 弘益大學校 科學技術硏究所 2001 科學技術硏究論文集 Vol.12 No.-

As the number of coal fired power plants in the domestic power industry continues to increases, the amount of fly ash produced is also increasing, making the reutilization of fly ash a vital issue. Various methods to reutilize the fly ash from coal power plants have been investigated, and one of such areas with the potential to grow is the usage as light weight sintered construction materials. Sintered fly ash aggregates are produced in two steps; the first is the pellet formation step, during which the fly ash particles containing unburnt carbons were made into a pellet using caking components, and the second the pellet sintering by the combustion heat of the carbons. During the sintering process, the combustion rate of the carbon affects the characteristics of sintered pellets. The combustion of carbon occurs via adsorption of O₂ on the active sites, and desorption of CO/CO₂. The parameters which affect the rate of adsorption, desorption, and transfer of O₂ and CO/CO₂ include the O₂ partial pressure and flow rate, the pellet size, the porosity, the pore tortuosity, the pore size distribution, the carbon content and distribution. This study investigated the combustion of carbon during the production of sintered fly ash through modeling. A simple combustion model using Langmuir isotherm is used to calculate the pellet temperature. The oxygen diffusion through the pores is ignored. At the temperatures of sintering, the desoption process is a rate limiting process, making the overall rate sensitive to the desoption kinetic parameters. In addition, it was found that the carbon content and the heat capacity of the pellet affected both the rate of the reaction and the final pellet temperature, while the area of active sites only affect the reaction rate. The future work will include the combustion modeling with external and internal diffusion of O₂, and the model confirmation through experimentation.

OB-63 : Positive effects of Teratogenic risk counseling in pregnant women inadvertently exposed to medications

( Hyo Yeon Gwak ),( Jung Eun Lee ),( Ju Young Choi ),( So Yeon Kim ),( Song Mi Park ),( June Seek Choi1,),( Hyun Kyong Ahn ),( Min Hyung Kim ),( Jin Hoon Chung ),( Moon Young Kim ),( Hyun Mee Ryu ),( 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: To evaluate the positive effects of teratogenic risk counseling in pregnant women inadvertently exposed to medication in early pregnancy. 방법: In a prospective cohort study, 250 pregnant women exposed to medications in early pregnancy were recruited after teratogenic risk counseling at Korean MotherSafe Counsling Center, which has given information about teratogenic risk of medication to pregnant women. Positive effects are evaluated by visual analogue scale(VAS) for perception on teratogenic risk and percent prefering termination of pregnancy after medication exposures in pregnancy. 결과: Mean age of participants is 31.8±5.3years old. Mean gravidity is 2.4±2.1. Medications exposed in early pregnancy include antibiotics, analgesics, anti-inflammatory drugs, antacid and so on. Mean perceptive teratogen risk before and after the counseling is 33.9±1.6% and 15.8±1.6%, respectively. And mean percent prefering termination of pregnancy (≥5) before and after the counseling is 31.1±1.9% and 13.5±1.7%, respectively. 결론: Our data suggest that information on teratogenic risk for pregnant women inadvertently exposed to medication may have positive effects for reassuring pregnant women and preventing termination of pregnancy.