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      • 인삼사포닌의 마우스 비장 임파구에 대한 면역활성

        정경수,이대우,양희정,이순철,김길현 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

        To ellucidate immunologic activities of ginseng saponin, the splenic leukocytes of BALB/c mice were incubated in RPMI medium for 48hr with or without ginseng total saponin(GTS), and then analyzed flow cytometrically. Upto the concentration of 100㎍/㎖, GTS exherted stimulatory effect on the immune cells most prominently on B cells: the percentage of the surface Immunoglobulin (sIg) -positive cells (B cells) of the control was 47.l%, while that of GTS treated was 58.9%. The percentages of monoclonal antibody(mAb) J1j-reactive cells (T cells) were 28.8% and 3l.3%, those of mAb Gkl.5-reactive cells (CD4' T cells) were l8.5% and l9.l%, and those of mAb M1/70-reactive cells (mostly macrophages) were 4.4 and 5.0% at the concentration of 0 and 100㎍/㎖, respectively. However, at the concentration of 300㎍/㎖ or higher, GTS was found to be toxic to the immune cells especially to B cells in that the flow rate of the cells (gated viable cells) significantly decreased and the percentage of B cells droped from 58.9% to 31.2% and 25.4% at l00, 300, and 1000㎍/㎖, respectivelly. These data strongly suggests that GTS would exhert B-cell mitogen-like activity at physiological concentrations of 100㎍/㎖ or lower.

      • 한국ㆍ대만ㆍ중국ㆍ조선 재무제표의 비교 연구

        정건영,최순희 계명대학교 산업경영연구소 2001 經營經濟 Vol.34 No.1

        This study is a comparative study of four different nations-Korea, Taiwan, China, DPRK-financial statements. It consists of financial statements preparing criteria and those forms and systems. This study found as follows. ①There are distinctive differences in financial statements between two different economic systems so called market economic system and planning economic system. ②Those differences include financial statements preparing criteria and those forms and systems along with elements and account classification of financial statements. ③There is also distinctive differences in elements and account classification of financial statements along with the difference of expense classification and revenue determination method between Chinese financial statements and DPRK's financial statements even if those nations have same socialist economic system as an accounting environment.

      • 킬레이트 수지에 의한 금속이온 흡착에 관한 연구

        정순량,양희태,오해범 又石大學校 1981 論文集 Vol.3 No.-

        The selective adsorption of metal ions by chelating agent-treated resin was studied in aqueous media. The resin was prepared by the treated conventional anion exchange resin, Dowex 1-X8(48 to 80 mesh) with chelating agent containing sulfonic groups such as 1, 2 -dihydroxy benzene- 3, 5 disulfonate(Tiron). The stability of the resin was markedly influenced by the pH of the media. The optimum conditions for the adsorption of metal ions were determined with respect to the pH, shaking time and mixture solution of metal ions. Under the optimum condition the other of the adsorption of metal ions is as follows: Fe(Ⅲ), Cu(Ⅱ), Ca(Ⅱ), Mg(Ⅱ), Ni(Ⅱ), and Zn(Ⅱ) are little. The total capacities of the resins in Fe(Ⅲ) were found 37. 98㎎/gr in the condition of pH 3 and in Cu 6.86㎎/gr in the condition of pH 3.5.

      • KCI등재

        골프장의 잔디 토양에서 살균제 Tolclofosmethyl의 분해

        정근욱,우선희,김흥태,사동민,김영기,홍순달,김재정,이재구 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3

        Tolclofosmethyl (TCFM) is heavily and annually applied to the turf soils of most golf courses in Gyeongju to control the fungi known to cause the disease brown patch. The soil samples used for the experiment was collected three weeks after the annual application at the end of May in the year 2002. The preliminary results obtained from this study demonstrated that the repeated field annual applications of TCFM to the turf soils of a golf course located in Gyeongju city in the southern area of Korea showed the enhanced degradation of the parent compound TCFM, especially in the surface (0~15 cm) soil rather than the shallow subsurface (15~30 cm) and deep subsurface (30~45 cm) soils, compared to the corresponding surface (0~15 cm) and shallow and deep subsurface (15~30 cm and 30~45 cm) soils of the untreated plot. It appears that microorganisms in the soil may be involved in the enhanced degradation of TCFM.

      • 제왕절개 자궁적출수술의 임상적 고찰

        정갑인,남계현,이해혁,이순곤,이권해,김동희 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

        Objective: This study was evaluated twenty-six cases of emergency Cesarean hysterectomy to analyse incidence, age distribution, parity, indications, complications, amount of blood transfusion and fetal morbidity. Methods and Materials : This study was carried out on twenty-six cases who underwent emergency Cesarean hysterectomy at department of obstetrics and gynecology, Soonchunhyang University Hospital from January 1987 to November 1997. Results: 1. The incidence of Cesarean hysterectomy was perfomed in 26 cases among total 19,277 deliveries(0.1%). 2. The indications of Cesarean hysterectomy were uterine atony, placenta previa, adhesive placenta and bleeding tendency. 3. There was matenal death in one case and main cause of death was massive bleeding due to disseminated intrabascular coagulopathy. 4. The complications of Cesarean hysterectomy were disseminated intravascular coagulopathy, bladder injury, postoperative infection, wound disruption and postoperative hemoperitoneum. 5. Two cases of fetal and neonatal mortality were intrauterine fetal death and neonatal death. Conclusion : The incidence of Cesarean hysterectomy was increased by increasing parity and maternal age.

      • KCI등재

        성폭력 피해여성의 경험에 관한 연구

        정연강,권혜진,지순주,남선영,김경희 韓國學校保健學會 1996 韓國學校保健學會誌 Vol.9 No.1

        This studies designed to work out a theoretical framework on the experience of sexual abuse from the perspective of grounded theory in an effort to provide more practical and efficient nursing intervention for female victims. The subcategories identified were "sexual abuse", "threatening", "absent mindness", "embarrassment", "horripilation", "dizziness", "wondrousness", "filthiness", "sexual curiousity", "violence level", "victim's age", "neighbors response", "victims personality", "common experience", "sexual abuse information", "family relations", "level of familiarity", "hiding", "suppression", "self-torture", "self-protection", 'avoidance", "asking aid", "withdrawal", "hatred", "confusion", "dodging", "remmant", and "pursuing". The 29 subcategories given above were further integrated into 16 categories such as "victimizedness", "being astounded", "filthiness", "degree", "developmental stage", "response pattern", "personality", "rarity", "infromation availability", "family support", "cover-up", "escaping", "informing", "negative internalization", and "positive pursuit of change". The core categories linked to all the other categories turned out to be "being taken aback" and "filthiness" incorporation the relevant subcategories. A total of 23 theoretical hypothesis emerged in the process of analyzing data. 1. the grater sexual curiosity, the weaker the senses of being taken aback and filthiness. 2. The weaker sexual curiosity, the stronger the senses of being taken aback and filthness. 3. The stronger the level of violence, The more violent the senses of being taken aback and filthiness. 4. The lower the level of violence, the weaker the senses of being taken aback and filthiness. 5. The younger the victims, the stronger the senses of being taken aback and filthness. 6. The older the victims, The weaker the senses of being taken aback and filthness. 7. 'Escaping' will transpire regardless of the given circumstances. 8. The weaker the senses of being taken aback and filthness, the more probable 'informing' and 'escaping' transpire. 9. The stronger the senses of being taken aback and filthness, the more probable 'informing' and 'escaping' transpire. 10. The more protective the response from 'informing' and 'escaping' transpire around, the more likely the response to 'being taken aback' and 'filthiness' will be 'informing' and 'escaping'. 11. The more repelling the response from around, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 12. The more open minded the personality of subject, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 13. The more closed the personality of subject, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 14. The more frequent the experience of sexual abuse, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 15. The less frequent the experience of sexual abuse, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 16. The more available information concerning sexual abuse, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 17. The less available information concerning sexual abuse, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 18. The more cohesive the family of the subject, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 19. The less cohesive the family of the subject, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 20. The more familiar the subject is with the abuser, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 21.The less familiar the subjec is with the abuser, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 22. The more likely the response to 'being taken aback' and 'filthines' is 'informing' and 'escaping', the more positive changes the subject will pursue. 23. The more likely the response to 'being taken aback' and 'filthiness' is 'covering-up' and 'escaping', the more negative changes the subject will pursue. The following four hypothneses were conformed in the process of data analysis. 1) In case the level of violence is strong but 'being taken aback' and 'filthiness' in weak because of strong sexual curiosity and also if information concerning sexual abuse is not readily available and the frequency is low, negative internationalization marked by 'covering-up' and 'escaping' will take place despite the fact the subject is open-minded, the family is cohesive and the abuser is unfamiliar. 2) In case the level of violence is weak but 'being taken aback' and 'filthiness' is weak combined wit weak sexual curiosty and also if information concerning sexual abuse is readily available and the response from around is protective and the frequency is high, the subject will pursue positive changes to 'being taken aback' and 'filthiness' , further aided by the fact that the subject is open-minded, the family is cohesive and the abuser is unfamilar. 3) In case the level of violence is strong but 'being taken aback' and 'filthiness' is strong because of weak sexual curiosity and also if information concerning sexual abuse is reading available and the response from around is readily available and the response from around is protecvice and the frequency is low, the subject will persue positive changes marked by 'informing' and 'escaping' despit the fact that the family cohesion is weak and the abuser is familiar. 4) In case the level of violence is strong but 'being taken aback' and 'filthiness' is strong because of weak sexual curiosity and also if information concerning sexual abuse is not readily available and the response from around is respelling and the frequnecy is low negative internalization like 'covering-up' and 'escaping' will take place, further aggravated by the fact that the subject's personality is closed, family cohension is weak, and subject is familiar. On the basis of the above finding, it is recommended that nursing intervention should focus on promoting the milieu conductive to the victims pursuing positive changes along with the adequate aids from protection facilities as well as from the people around them.

      • Pseubomonas aeruginosa의 Temperature Sensitive 突然變異體의 유도와 분리

        유관희,정기흥,정순정,이형환 건국대학교 1983 論文集 Vol.16 No.1

        Sixty-one temperature-sensitive mutants of Pseudomonas aeruginosa have been isolated from the mutagenized culture with N-methyl-N'-nitro-Nitrosoguanidine. The mutagen was treated at 36℃ and the ts-mutants were isolated at the non-permissive temperature of 42℃ by size of colony and growth. Twenty-five mutants were smaller in the size of colonies at 42℃ than those of 36℃, and thirty-sin mutants were not growed at 42℃.

      • sfs1 유전자의 cAMP-cAMP receptor protein에 의한 발현 조절

        이희영,이승진,유주순,최용락,정수열 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

        crp* 유전자가 도입된 대장균 MK2001(crp, cya::km)을 숙주로 사용하여 mal 유전자 발현을 촉진시키는 유전자의 하나인 sfs1(sugar fermentation stimulation)의 구조해석 결과에 의하면, 잠정적인 sfs1의 promoter영역에는 CRP단백질과의 결합영역으로 보이는 염기배열이 존재하였다. 본 실험에서는 sfs1 유전자의 cAMP-CRP에 의한 발현 조절을 확인하고자, lacZ와의 융합 유전자를 작성하였다. 작성된 융합 유전자는 cya결손주인 TP2010에서 cAMP의 첨가에 의해 B-galactosidase 활성이 크게 증가하였으며, Western blotting의 실험에서도 같은 결과를 나타냈다. in vivo에서 발현이 확인된 전사산물은 cAMP에 의해 전사 촉진이 일어났으며, CRP의 결합부위로 예상되는 DNA영역은 cAMP가 존재하면 CRP단백질과 결합하는 특성을 나타내었다. 이상의 결과로 보아, sfs1 유전자의 발현은 cAMP-CRP에 의한 전사촉진 현상을 받는 것으로 나타났다. We have cloned several E. coli sfs genes which stimulate mal gene expression with ??. One the sfs genes(pPVC2) was sequenced and potential CRP binding site is located in the upstream of the putative promoter in the regulatry region. In order to investigate the regulation of the sfs1 gene by the cAMP-CRP complex, we have constructed the sfs-lacZ fusion gene in this research. The overall transcriptional stimulations of sfs1 gene in the presence cAMP were confirmed by B-galactosidase activity and Western blot analysis of sfs1-lacZ fusion gene. Transcriptional regulation by cAMP-CRP was also confirmed by Northern blot analysis. End-labelled DNA of the DNA fragement in sfs1 regulatory region were used for gel retardation assay to examine the CRP-DNA complex in the presence of cAMP. Results here indicate that CRP binding site in the regulatory region of sfs1 gene is positive regulator for the expression of sfs1 gene.

      • 간호기록분석에 의한 성인환자의 간호진단 확인연구

        김복순,이정희 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.2

        The purpose of this study is to diagnose the nursing problem by analizing nursing records and to give the qualified nursing service with scientific approach to the hospitalized medical patients. The subject for this paper were 507 out of 1805 who bad one or more admission history a, medical department, from CAU hospital, locating in Seoul, from Jan. 1. 1983 to Dec. 31. 1983. For the study, it is used the Nursing Diagnoses edited by Mi Ja Kim et al. and divided nursing need into several parts with the nursing diagnosis catalog The results of this study are as follows: 1. The number of nursing diagnosis from the discharge records are 3314 and the average number of nursing diagnosis per patient is 6.5 2. Determined nursing diagnosis from 507 discharge records is(Comfort, Alteration in) 91.2%, (Powerlessness) 59%, (Nutrition, Alterations in: Less than body requirment) 38.3%, (Breathing patterns, Ineffective) 34.9%, (Anxiety) 31.6%. and(Sleep pattern Disturbances) 29%. 3. Applying the division of Nursing Diagnosis Catalog to Maslow's hierachy of Need Majorityof Nursing Diagnoses belongs to Physiological need 71%, next is Safety need 11%, Belonging and Love need 6.6% and Esteem and Self actualization need 11.4%. Maslow's each stage by Nursing Diagnoses shows high; (Comfort, Alteration in) to Physiological need stage, (Anxiety) to Safety need stage, (Coping, Ineffective Individual)to Belonging and Love need stage, (Diversional Activity, Deficit) to Esteem and Self actualization need stage.

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