몇가지 항생제의 느타리버섯 Pseudomonas tolaasii에 의한 세균성갈색무늬병 방제 효과

서건식 외 한국버섯학회 2003 한국버섯학회지 Vol.1 No.1

느타리버섯 세균성갈색무늬병 방제 약제를 선발하기 위하여 33종의 항생제를 공시하여 P. tolaasii에 대한 항균활성을 조사하고 선발된 4종의 항생제를 이용하여 방제효과를 검정한 결과는 다음과 같다. Kanamycin등 19개의 항생제가 병원균 P. tolaasii에 대하여 항균활성을 나타내었으며, 그 중에서 tetracycline, streptomycin, kanamycin과 kasugamycin이 강한 항균활성을 나타내었다. Tetracycline과 kanamycin은 10ppm에서도 세균생장을 완전히 억제시켰고 Kasugamycin은 100ppm, streptomycin은 200ppm 이상의 농도에서 병원세균에 항균활성을 보였다. Tetracycline, kanamycin, kasugamycin, streptomycin을 이병균상에 처리한 결과 각각 72.9, 71.2, 68.1 그리고 62.7%의 방제가를 보였다. tetracycline 처리구의 느타리버섯 생산량은 무처리구보다 31.8% 이상의 증수효과를 보였다. 항생제 처리가 느타리버섯 균사생장에 미치는 영향을 조사한 결과 kanamycin을 처리한 배지에서는 항생제 처리의 영향을 전혀 받지 않았으며 오히려 균사생장을 촉진하는 것으로 밝혀졌다. Streptomycin을 처리한 배지에서는 항생제 무처리 배지에서와 동등한 수준의 균사생장을 보였다. Tetracyclin의 경우는 무처리에 비해 약 10에서 20%의 균사 생장 억제를 보였으나 Kasugamycin 100ppm 이상의 농도에서는 약 40%의 균사생장 억제를 보였다. Antibacterial activities of 33 antibiotics against Pseudomonas tolaasii causing the brown blotch disease on the edible mushroom Pleurotus ostreatus, were tested in vitro for the control of the disease. Tetracyclin, kanamycin, kasugamycin, and streptomycin showed strong antibacterial activity against P. tolaasii, having the minimal inhibitory concentration of 10,10,100 and 200ppm, respectively. These antibiotics showed similar control value of 72.9,71.2,68.1 and 62.7%, respectively when applied on the artificially infected mushroom beds. Mushroom yields in the tetracycline treated boxes were increased about 31.8% comparing to the control ones. Mycelial growth of P. ostreatus on the PDA supplemented with streptomycin and kanamycin were not affected, but were inhibited 10~20% and 40% with tetracyclin and kasugamycin treatment, respectively.

정신분열병 및 정신분열형 장애에 대한 올란자핀의 효과와 안전성

안용민,강대엽,권준수,김창윤,김철응,반건호,신영민,이기철,이동우,이중서,조현상,채정호,김용식 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : 본 다기관 공동임상연구는 국내 환자를 대상으로 새로운 항정신병약물인 올라자핀의 치료 효과와 안전성을 확인하고자 시행되었다. 방 법 : 1999년 7월부터 2000년 3월까지 국내 10개 병원에 방문한 정신분열병 및 정신분열형 장애 105명을 대상으로 비대조 개방 임상연구를 시행하였다. 중등도 이상의 정신병 증상을 지니거나, 부작용으로 약물교체가 필요한 경우 또는 기분장애 증상의 치료가 필요한 환자를 대상으로 하였다. 2∼7일간의 약물 배설기간 후에 1일 10mg의 올란자핀을 투여하였고, 이후로 3∼7일 간격으로 용량을 조절하여 총 8주간 투여하였다. 치료 효과는 PANSS(Positive and Negative Syndrome Scale), BPRS(Brief Psychiatric Rating Scale), CGI(Clinical Global Impression-Severity), MADRS(Montgomery-Asberg Depression Rating Scale), HAM-A(Hamilton Rating Scale for Anxiety)로 판정하였다. 안전성 평가는 자발적인 이상반응 보고, 활력징후와 혈액 검사 및 SAS(Simpson-Angus Scale), BARS(Barnes Akathisia Rating Scale), AIMS(Abnormal Involuntary Movement Scale)척도를 이용하였다. 결 과 : 105명중에서 85.7%인 90명이 8주간의 치료를 완료하였다. 평균 최빈 용량은 일일 16.1(±4.7)mg이었고, 종료시점에서의 BPRS전체 점수가 기저 상태에 비해 40%이상 감소된 반응군은 69.5%이었다. PANSS의 양성 증후군과 일반정신병리 뿐만 아니라 음성 증후군 소척도에서도 유의한 감소를 보였으며, 이 감소들은 치료 초기부터 나타나서 8주간 지속되었다. 43.8%의 피험자가 중등도 이상의 우울증상을 같이 지니고 있었으며, 올란자핀에 의해 MADRS와 HAM-A가 유의하게 감소하였다. 치료 기간 동안에 활력징후에는 뚜렷한 변화가 없었으나 체중은 8주간 지속적으로 증가하였다. SAS와 AIMS는 감소하였으며, 정좌불능증을 제외한 나머지 추체외로 증상의 발생비율도 낮았다. 임상적인 증상이나 징후를 동반하지 않는 ALT/SGPT의 상승을 보였지만 프로락틴을 포함하는 대부분의 혈액 검사상 뚜렷한 이상 변화는 없었다. 결 론 ; 비록 본 임상연구가 비대조 개방 임상연구로서 많은 제한점을 가지고 있지만, 올란자핀이 기존 약물에 내약성을 보이거나 기분증상을 동반하는 정신병에 치료 효과가 있었다. 그리고 기존 항정신병약물 치료시에 문제가 되었던 추체외로 증상과 고프르로락틴 혈증을 포함하는 대부분의 부작용면에서 안전성을 보여 주었다. Objective : This multicenter clinical trial was carried out to investigate the efficacy and the safety of olanzpine for the treatment of Korean patients. Mothod : 105 patients with schizophrenia and schizophreniform disorder, visited at 10 mental or university hospitals, had received an open and non-comparative treatment with olanzapine for 8 weeks. Patients had psychotic or depressive symptoms with the severity above moderate degree or intolerable side effects to previous antipsychotics. After a wash-out period of 2-7 days, 10mg olanzapine was prescribed initially to all the patients, and then the dosage could be adjusted within the range of 5-20mg/day of olanzapine by 3-7 days. Results : 90(85.7%) of 105 patients completed the 8-weeks trial and the mean modal dose of olanzapine was 16.1(±4.7%)mg/day. At the end of the trial, 73 patients(69.5%) were classified as responder, which was defined as 40% or more improvement in BPRS(Brief Psychiatric Rating Scale) score comparing to baseline. There was a significant reduction in the scores of PANSS(Positive and Negative Syndrome Scale)and subscales including negative symptom scores and CGI. Also weekly analysis showed that the reductions in scores were kept on for the whole period of the trial. 43.8% of all the patients had depressive symptoms at the baseline and total scores of MADRS(Montgomery-Asberg Depression Rating Scale) and HAM-A(Hamilton Rating Scale for Anxiety) were also reduced after the trials. Vital signs revealed no clinically significant changes but continuous weight gain was observed during the treatment with olanzapine. The scores of SAS(Simpson-Angus Scale) and AIMS(Abnormal Involuntary Movement Scale) for assessing the EPS(extrapyramidal symptoms)and tardive dyskinesia respectively were significantly decreased and only a few patients reported EPS as adverse events. Although mild and clinically non-significant of ALT/SGPT was observed, most laboratory parameters including plasma prolactin level showed to significant changes during the trial. Conclusions : Although this trial had many limitations because it was a non-comparative and open study, olanzapine showed high efficacy on the positive, negative and depressive symptoms in schizophrenia and schizophreniform disorder.In addition to that, olanzapine showed a substantially favorable safety profile, such as low incidence of EPS and hyperprolactinemia.

Effect of concentrated carbon dioxide exposure on the mycelial growth and fruit body initiation of Ganoderma lucidum.

Geon Sik Seo,Akira Suzuki 한국버섯학회 2004 한국버섯학회지 Vol.2 No.2

The effect of CO2 concentration (500, 3,000, 6,000㎕/ℓ) on the mycelial growth and fruit body primordium formation of Ganoderma lucidum on nutrient agar medium was examined. Optimum CO2 concentration for vegetative growth was above 3,000㎕/ℓ. Fruit body initiation was accelerated at higher than 3,000㎕/ℓCO2 exposure but the maximum number and size of primordia, and primordium color were not influenced by CO2 concentrations. Whereas each atypical fruiting structure forming stock culture showed different fruiting time under each concentration of CO2 exposure.

Effect of light on fruit body primordium formation of Ganoderma Iucidum on nutrient agar medium

Seo, Geon-sik,Otani, Hiroshi,Yu, Seung-Hun,Keisuke, Kohmoto 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

Phospholipase A_2(PLA_2)는 생체막의 주요 구성성분인 인지질의 sn-2위치에서 지방산 ester 결합을 가수분해하는 효소로 염증과 관련된 질병에 있어서 매우 중요한 역할을 하는 것으로 알려졌다. 본 연구에서는 항염증 치료제의 개발을 목표로 PLA_2의 특이적 저해물질을 탐색하던 중 Umbilicaria esculenta(石耳)의 메탄을 추출물로부터 PLA_2 저해활성을 갖는 두 화합물 G2, G3을 분리하였다. 활성물질의 분리는 U. esculenta의 메탄올 추출물을 에틸아세테이트로 추출하고 실리카 젤 컬럼, 크로로포름 추출, preparative TLC 등에 의하여 정제하였다. 각종 스펙트럼 분석 및 ^1H, ^13C, DEPT 등의 NMR 실험에 의하여 G2 화합물은 분자량 124, 분자식 C_7H_8O_2의 5-methyl-1,3-benzendiol(orcinol), G3 화합물은 분자량 182, 분자식 C_9H_10O_4의 2,4-dihydroxy-6-methyl-benzoic acid methyl ester(methyl orsellinic acid) 페놀성 화합물로 동정하였다. 본 화합물의 PLA_2 저해활성은 IC_50값이 G2의 경우 0.22 mM, G3의 경우 0.26 mM이었다. 그후 이 두화합물의 in vivo 염증모델에서 그 효능을 검토할 예정이다. Two depsides, medicinal herb products isolated from the methanol extract of Umbilicaria esculenta, inhibited human synovial fluid Phospholipase A_2 (PLA_2) (IC_50 of 0.22 and 0.26 mM, respectively). In the course of screening for antiinflammatory compounds from natural products, we successfully isolated two depsides PLA_2 inhibitory compounds, Orcinol nad methyl orsellinic acid. The compounds were indentified as orcinol and methyl orsellinic acid on the basis of various NMR studies including ^1H, ^13C and DEPT experiments.

Morphological and molecular characterization of Hypocrea sp. isolated from oyster mushroom bed in Korea

Geon Sik Seo,Byung-Ryun Kim,Myung-Soo Park,Min-Kyung Kim,Seung-Hun Yu 한국버섯학회 2004 한국버섯학회 국제학술대회 Vol.3 No.-

느타리버섯 유해균 Hypocrea sp. 의 형태 및 URP - PCR 분석

서건식(Geon Sik Seo),김병련(Byung Ryun Kim),박명수(Myeung Soo Park),김민경(Min Kyung Kim),유승헌(Seung Hun Yu) 한국균학회 2002 韓國菌學會誌 Vol.30 No.2

N/A

이산화탄소 농도가 영지버섯균의 균사생장과 자실체원기 유도에 미치는 영향

서건식,鈴木彰 한국버섯학회 2004 한국버섯학회지 Vol.2 No.2

The effect of CO₂ concentration (500, 3,000, 6,000㎕/ℓ) on the mycelial growth and fruit body primordium formation of Ganoderma lucidum on nutrient agar medium was examined. Optimum CO₂ concentration for vegetative growth was above 3,000㎕/ℓ. Fruit body initiation was accelerated at higher than 3,000㎕/ℓ CO₂ exposure but the maximum number and size of primordia, and primordium color were not influenced by CO₂ concentrations. Whereas each atypical fruiting structure forming stock culture showed different fruiting time under each concentration of CO₂ exposure.

Ganoderma lucidum(Fr.) Karst의 培養的 特性에 關한 硏究

徐健植,申寬澈,朴鍾聲 충남대학교 농업과학연구소 1988 農業技術硏究報告 Vol.15 No.1

In order to elucidate the systematic taxonomy and genetic characters of Canoderma lucidum, cultural characteristics of the fungus were investigated. Mycelial growth of Ganoderma lucidum were favorable on oat meal agar medium, and optimum temperature and pH of the medium for mycelial growth were 30℃ and 5.5-6.0 respectively. Irradiation of white fluorescent lamp inhibited mycelial growth and critical time for inhibition of mycelial growth was 4-8 hours. Concentric zones and mycelial strands of Ganoderma lucidum was induced by irradiation of white fluorescent lamp and formation of mycelial sectors was influenced by nutrient source of media and irradiation of white fluorescent lamp. These characters were different among the isolates, but no relationship was observed between these characters and the fruiting body type of the fungus. Basidiospores were formed directly from the mycelium cultured on artificial media without producing fruit body.

韓國産 自生 靈芝 버섯(Ganoderma lucidum)의 培養的 特性에 關한 硏究

徐健植 충남대학교 대학원 1989 論文集 Vol.10 No.-

In order to elucidate the systematic taxonomy and genetic characters of Ganoderma lucidum, cultural characteristics of the fungus were invstigated. Mycelial growth of Ganoderma lucidum were favorable on oat meal agar medium, and optimum temperature and pH of the medium for mycelial growth were 30℃ and 5.5-6.0 respectively. All practical carbon sources for mycelial growth of Ganoderma lucidum were effective. Ammonium nitrate, ammonium tartrate and sodium nitrate were suitable as nitrogen sources. The mycelial growth of Ganoderma lucidum on the media with hystidine were good but variation among the isolates was very extensive. Light exposure to Ganoderma lucidum inhibited the mycelial growth and its effect was different by lighting time and the fungus isolates. The longer time and lighting, the higher the inhibitory effect on mycelial growth. The limit time of growth inhibition was 4 to 8 hours light expoure dairly at 500-4,000lux. The mycelial growth of Ganoderma lucidum showed no difference by the light intensity. The mycelial growth of the fungus was strongly retarded by N. U. V. but red light affected very low.

백색자실체를 형성하는 영지 균주의 비정형 자실체 구조의 형성

서건식 한국균학회 1999 韓國菌學會誌 Vol.27 No.5

Five white fruitbodies of Ganoderma lucidum found from two different mushroom farms, and the characteristics of atypical fruiting structure formation of these strains were described. The white fruitbodies were spontaneously generated on Quercus-log during the cultivation. They did not differentiate to the normal fruitbodies with pileus, hymenium, stipe and coloration, and fruitbodies remained non-laccateed even after 3 months. Dikaryotic mycelia isolated from the five white fruitbodies differed from wild-type strains in the mycelial growth rate, colony color, and the capacity of atypical fruiting structure (AFS) formation on agar media. These white mutants readily induced brown colored AFSs on the colonies under ventilation and illumination conditions. Both isolates Gl-010 and Gl-011 that were obtained from a normal and white fruitbody, respectively, did not form AFSs in the dark and/or under black light blue (BLB) light illumination, but induced under the visible light. They required dim light for the AFS formation, and the AFS formation was inhibited up to 0.5 μ㏖ m^(-2) s^(-1) in light intensity. However, the other four isolates induced AFSs even in the dark and BLB illumination, although their parent strain, isolate Gl-030, did not form AFSs under any light conditions. The monokaryotic mycelia derived from basidiospores of the AFSs of the white mutants were compatible with the original culture (dikaryon) on a dual culture.