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백창렬,이동건,최정현,정현화,조유경,박훈준,이승훈,박윤희,이교영,민우성,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4
As a result of the enlarging pool of unvaccinated children and young adults, there has been an increase in measles in our countries. In these situation, it has been reported that measles associated pneumoinia is easily complicated with fatal respiratory failure, espycially in immunocompromised patients. Herein we report the case of lethal measles pneumonia after allogenic hematopoietic stem cell transplantation in adults proven by autopsy. Recently, one case of measles was encountered in 39-year-old female patients after allogenic bone marrow transplanted case (chronic myelogenous leukemia), who progressed into interstitial pneumonia pattern, despite treatment including antibiotics, immunoglobulin. The patient died of giant cell pneumonia compatible with that of measles which was comfirmed in the section of necropsy lung specimen. (Korean J Infect Dis 33:301∼309, 2001)
균일 열유속 조건의 원기둥에 의한 자연대류에서 원기둥의 경사각이 열전달특성에 미치는 영향
芮龍澤,秋弘錄,方昌焄 慶一大學校 1997 論文集 Vol.14 No.2
Natural convection heat transfer of circular cylinder is investigated experimentally. The main purpose of this study is to investigate the effect of inclined angles of the cylinder on natural convection characteristics. The cylinder is made of aluminum, and air is used as the cooling fluid. In this experiments, local temperature measurements are made along the surface of the cylinder. In this study, results are obtained for heat flux of 150 W/m2∼600 W/m2, inclined angle of 0o∼90o. Results indicate that the local heat transfer coefficient is increased with the increasing of heat flux at the constant inclined angle. Also, at the same heat flux, the local heat transfer coefficient is increased with the increasing of inclined angle from the vertical position.
Jae-Sung Ryu,Jeong-Woong Lee,Dong Hoon Kwak,Ji-Su Kim,in-Hyoung Cho,So-Dam Lee,So-Hyun Lee,Ju-Taek Lee,Kyu-Tae Chang,Young-Kug Choo 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Gangliosides are complex glycosphingolipids that contain one or more sialic acids, the major components of cytoplasmic cell membranes. These are thought to play a role in the control of biological process including cell surface interaction, cell differentiation and transmemebrane signaling. In this study, we demonstrated that change of gangliosides pattern and glycosyltransferase gene in mouse induced pluripotent stem cells (miPSCs). The miPSCs were derived from OG2 mouse embryonic fibroblasts (mEFs) using retrovirus-mediated delivery and expression of Oct-4, Sox-2, Klf-4 and c-Myc. These cells were expressed alkaline phosphatase (AP), stage specific embryonic antigen-1 (SSEA-1) and mRNA such as Oct-4, Sox-2 and Nanog. In HPTLC and immunochemistry analysis, ganglioside GM3 and GD1a were expressed in mEFs, miPSCs and mESCs. However, only GM2 and GM1 were expressed in mEFs and mESCs. Ganglioside GM3 and GD3 expression level was low in miPSCs. Moreover, A2B5 antigen, c-series gangliosides such as GT3 and GQ1c, was only expressed in mESCs. To provide a better understanding of the metabolic basis of glycosyltransferase genes expressions, we analyzed expression of glycosyltransferase genes in mEFs, miPSCs and mESCs, and found that expression of sialyltransferase-I (ST-I), ST-II, ST-III, N-acetylgalactosamine transferase (GalNAcT), galactosyltransferase-II (Gal-II), ST-IV and ST-V. However, ST-II was not only expressed in mEFs. These results suggest that ganglioside GM3 and GD1a unique and powerful cell-surface biomarker to identify of mESCs and miPSCs, and change of ganglioside expression could be affected by gangliosides synthase activation. Therefore, gangliosides may play a role in the cell differentiation process.
Kiyuk Chang,Youngkeun Ahn,Sungmin Lim,Jeong Hoon Yang,Kwan Yong Lee,Eun Ho Choo,Hyun Kuk Kim,Chang-Wook Nam,김원,Jin-Yong Hwang,Seung-Woon Rha,Hyo-Soo Kim,Myeong-Chan Cho,Yangsoo Jang,Myung Ho Jeong 대한심장학회 2021 Korean Circulation Journal Vol.51 No.4
Acute myocardial infarction (AMI) is a fatal manifestation of ischemic heart disease and remains a major public health concern worldwide despite advances in its diagnosis and management. The characteristics of patients with AMI, as well as its disease patterns, have gradually changed over time in Korea, and the outcomes of revascularization have improved dramatically. Several characteristics associated with the revascularization of Korean patients differ from those of patients in other countries. The sophisticated state of AMI revascularization in Korea has led to the need for a Korean expert consensus. The Task Force on Expert Consensus Document of the Korean Society of Myocardial Infarction has comprehensively reviewed the outcomes of large clinical trials and current practical guidelines, as well as studies on Korean patients with AMI. Based on these comprehensive reviews, the members of the task force summarize the major guidelines and recent publications, and propose an expert consensus for revascularization in patients with AMI.
Jin Hyoung Cho,Kyu Tae Chang,Young Choon Lee,Keon Bong Oh,Dong Hoon Kwak,Ji Su Kim,Jae Sung Ryu,So Dam Lee,So Hyun Lee,Ju Taek Lee,Young Kug Choo 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Gangliosides are ubiquitous component of the membranes of mammalian cells that have been suggested to play important roles in various cell functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. However, the role that gangliosides play in immune rejection response by xenotransplantation is not yet clearly understood. In this study, regulatory roles of human leukocyte in the ganglioside expression of the primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To study whether human leukocyte are related to expressional changes of gangliosides in PAECs we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with fetal bovine serum (FBS), as control, or with recombinant human tumor necrosis factor-α (TNF-α), or with human serum and FBS contained with human leukocyte. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS are contained GM3, GM1 and GD3 as major gangliosides. However, ganglioside GM1 significantly decreased in PAECs incubated for 5hrs with TNF-α (10 ng/ml), or with 10% human serum and FBS contained with human leukocytes. Taken together, these results suggest that human TNF-α secreted by human leukocyte against PAECs and possibly happened expressional pattern changes of ganglioside GM1 in PAECs by secreted TNF-α. The finding that human leukocyte promotes expressional pattern changes of ganglioside in PAECs depending on TNF-α secreation may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.
Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells
Jin-Hyoung Cho,Ji-Su Kim,Malg-Um Lim,Hyun-Ki Min,Dong-Hoon Kwak,Jae-Sung Ryu,Ju-Taek Lee,Sun-Uk Kim,Chang-Hwan Kim,Chang-Hyun Kim,Deog-Bon Koo,Kyu-Tae Chang,Young-Kug Choo 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.4
Gangliosides are ubiquitous components of the membranes of mammalian cells that are thought to play important roles in various cell functions such as cell-cell interaction, cell adhesion, cell differentiation, growth control, and signaling. However, the role that gangliosides play in the immune rejection response after xenotransplantation is not yet clearly understood. In this study, the regulatory effects of human leukocytes on ganglioside expression in primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To determine the impact of human leukocytes on the expression of gangliosides in PAECs, we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with FBS, FBS containing human leukocytes, human serum containing human leukocytes, and FBS containing TNF-α. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS predominantly express the gangliosides GM3, GM1, and GD3. However, the expression of GM1 significantly decreased in PAECs incubated for 5 h with TNF-α (10 ng/mL), 10% human serum containing human leukocytes, and 10% FBS containing human leukocytes. Taken together, these results suggest that human leukocytes induced changes in the expression profile of ganglioside GM1 similar to those seen upon treatment of PAECs with TNF-α. This finding may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.
Choo, Eun Ho,Han, Eun-Ji,Kim, Chan Joon,Kim, Sung-Hoon,O, Joo-Hyun,Chang, Kiyuk,Seung, Ki-Bae The Korean Society of Cardiology 2018 Korean Circulation Journal Vol.48 No.7
<P><B>Background and Objectives</B></P><P>Non-statin therapy plus lower intensity statin might be an alternative in patients with coronary artery disease (CAD). A recent data suggested an anti-inflammatory therapy can reduce recurrent cardiovascular events and pioglitazone is also an intriguing inflammatory-modulating agent. However, limited data exist on whether pioglitazone on top of statins further attenuates plaque inflammation.</P><P><B>Methods</B></P><P>Statin-naïve patients with stable CAD and carotid plaques of ≥3 mm were randomly prescribed moderate dose atorvastatin (20 mg/day), or moderate dose atorvastatin plus pioglitazone (30 mg/day) for 3 months. The primary endpoint was the change in the arterial inflammation of the carotid artery measured by <SUP>18</SUP>F-fluorodeoxyglucose positron emission tomography/computed tomography (<SUP>18</SUP>F-FDG-PET/CT) during 3 months.</P><P><B>Results</B></P><P>Of the 41 randomized patients, 33 underwent an evaluation by fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT; 17 atorvastatin plus pioglitazone and 16 atorvastatin patients). The addition of pioglitazone significantly improved the insulin sensitivity and increased the high-density lipoprotein cholesterol after 3 months. Although a reduction in the (FDG) uptake by pioglitazone on top of atorvastatin in carotid arteries with plaque showed marginally statistical significance in the entire patient group (atorvastatin plus pioglitazone; −0.10±0.07 and atorvastatin −0.06±0.04, p=0.058), pioglitazone showed a further reduction of the fluorodeoxyglucose (FDG) uptake among patients who had a baseline FDG uptake above the median (atorvastatin plus pioglitazone; −0.14±0.04 and atorvastatin −0.03±0.03, p<0.001).</P><P><B>Conclusions</B></P><P>Pioglitazone demonstrated marginally significant anti-inflammatory effects in addition to moderate dose atorvastatin. This may have been due to the lack of power of the study. However, pioglitazone may have an anti-inflammatory effect in those patients with high plaque inflammation (Trial registry at ClinicalTrials.gov, NCT01341730).</P>
Choo, Eun Jeong,Rhee, Yun-Hee,Jeong, Soo-Jin,Lee, Hyo-Jung,Kim, Hyun Seok,Ko, Hyun Suk,Kim, Ji-Hyun,Kwon, Tae-Rin,Jung, Ji Hoon,Kim, Jin Hyoung,Lee, Hyo-Jeong,Lee, Eun-Ok,Kim, Dae Keun,Chen, Chang-Yan Pharmaceutical Society of Japan 2011 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.34 No.1
<P>Anethole is known to possess anti-inflammatory and anti-tumor activities and to be a main constituent of fennel, anise, and camphor. In the present study, we evaluated anti-metastatic and apoptotic effects of anethole on highly-metastatic HT-1080 human fibrosarcoma tumor cells. Despite weak cytotoxicity against HT-1080 cells, anethole inhibited the adhesion to Matrigel and invasion of HT-1080 cells in a dose-dependent manner. Anethole was also able to down-regulate the expression of matrix metalloproteinase (MMP)-2 and -9 and up-regulate the gene expression of tissue inhibitor of metalloproteinase (TIMP)-1. The similar inhibitory effect of anethole on MMP-2 and -9 activities was confirmed by zymography assay. Furthermore, anethole significantly decreased mRNA expression of urokinase plasminogen activator (uPA), but not uPA receptor (uPAR). In addition, anethole suppressed the phosphorylation of AKT, extracellular signal-regulated kinase (ERK), p38 and nuclear transcription factor kappa B (NF-κB) in HT-1080 cells. Taken together, our findings indicate that anethole is a potent anti-metastatic drug that functions through inhibiting MMP-2/9 and AKT/mitogen-activated protein kinase (MAPK)/NF-κB signal transducers.</P>