Cofirmation on taxonomic status of Spatoglossum pacificum Yendo(Dictyotaceae, Phaeophyceae) based on morphology and plastid protein coding rbcL rbcS, psaA, and psb A gene sequences

황일기,김형섭,Wook Jae Lee 한국조류학회I 2004 ALGAE Vol.19 No.3

Phenological, morhological and molecular characteristics of Spatolossum pacificum Yendo are examined. S. pacificumhas an annual life cycle composed of sporophytes with an apparent absence of male and female gametophytes in Korea. The seasonal growth of this species explains that the annual growth is closely related to the monthly variation of water temperature. S. pacificum has protruding reproductive structures above the outmost cortical layer. Although thisobservation is restricted to several species, this reproductive organ on the thallus can make S. pacificum distinguishable from S. crassum and S. lactum. The morphogenesis of a midrib at the base of S. pacificum in this study is the same as those of Dictyopteris but different from those of S. crassum and S. lactum, suggesting that S. pacificum is closely related to Dictyopteris. In the comparison of plastid gene sequences among species of Spatoglossum and Dictyopteris, S. pacificum ismore similar to D. divaricata and D. undulata than those of S. crassum in rbcL, rbcS, psbA, and psaA. This resultis congruent with the anatomical characteristic of a midrib at the base of the thallus and the protrusion of reproductive organs on the thallus. The phylogenetic relationship based on these plastid genes also shows that S. pacificum is included in Dictyotpteris clade and separated from S. crassum. We transfer Spatoglossum pacificum Yendo to Dictyopteris Lamouroux based upon differences of anatomical characteristics of the midrib, the existence of reproductive organs on thallus and the molecular analyses.

Intraspecific variation of gene structure in the mitochondrial large subunit ribosomal RNA and cytochrome c oxidase subunit 1 of Pyropia yezoensis (Bangiales, Rhodophyta)

황일기,김승오,황미숙,박은정,하동수,이상래 한국조류학회I 2018 ALGAE Vol.33 No.1

Red algal mitochondrial genomes (mtDNAs) can provide useful information on species identification. mtDNAs of Pyropia / Porphyra (Bangiales, Rhodophyta) have shown diverse variation in their size and gene structure. In particular, the introns and intronic open reading frames found in the ribosomal RNA large subunit gene (rnl) and cytochrome c oxidase subunit 1 gene (cox1) significantly vary the mitochondrial genome size in Pyropia / Porphyra species. In this study, we examined the exon / intron structure of rnl and cox1 genes of Pyropia yezoensis at the intraspecific level. The combined data of rnl and cox1 genes exhibited 12 genotypes for 40 P. yezoensis strains, based on the existence of introns. These genotypes were more effective to identify P. yezoensis strains in comparison to the traditional DNA barcode cox1 marker (5 haplotypes). Therefore, the variation in gene structure of rnl and cox1 can be a novel molecular marker to discriminate the strains of Pyropia species.

Morphological characteristics of brown alga Spatoglossum crassum Tanaka(Dictyotaceae, Dictyotales), new to Korea

황일기,김형섭,Wook Jae Lee 한국조류학회I 2004 ALGAE Vol.19 No.3

The morphological and molecular characteristics of Pachydictyon coriaceum (Holmes) Okamura (1899) were described from Korea. Plants of this species were collected all year round and have maximum height from August to September. The monthly variability of thallus growth is in the way with that of the seawater temperature. Two types of thallus structures, thick cortical layer tallus type and thin cortical cell layer type, distinguished according to growing seasons. The habit of Korean plants is also classified into two thallus types, slender type and wide type,based on the length and the width of internodes, but this distinction between two types is not supported by either anatomical or molecular characteristics. P. coriaceum share typical morphology in branching pattern and morphogenetic processes with the other species of Dictyota: 1) have multi-cellular cortical and medullar layer in the partial of thallus, 2) have same the development of thallus from apical meristem cell, and 3) have the sub-lineage within Dictyotaspecies lineage in rbcL, psaA, and psbAgene sequences analyses. These characteristics lead to the conclusion that P. coriaceum should be transfer to the genus Dictyota.

Evidence for Taxonomic Status of Pachydictyon coriaceum(Holmes) Okamura(Dictyotales, Phaeophyceae) Based on Morphology and Plastid Protein Coding rbcL, psaA, and psbA Gene Sequences

황일기,김형섭,Wook Jae Lee 한국조류학회I 2004 ALGAE Vol.19 No.3

The morphological and molecular characteristics of Pachydictyon coriaceum (Holmes) Okamura (1899) are described. Plants are collected from Korea all year round and have maximum height from August to September. The monthly variability of thallus growth is in the way with that of the seawater temperature. Two types of thallus structures, thick cortical layer tallus type and thin cortical cell layer type, are distinguished according to growing seasons. The habit of Korean plants is also classified into two thallus types, slender type and wide type, based on the length and the width of internodes, but this distinction between two types is not supported by either anatomical or molecular characteristics. P. coriaceum shares typical morphology in branching pattern and morphogenetic processes with the other species of Dictyota: 1) multi-cellular cortical and medullar layer in the partial of thallus, 2) same development of thallus from apical meristem cell, and 3) sub-lineage within Dictyota species lineage in rbcL, psaA and psbA gene sequences analyses. These characteristics lead to propose the new combination of Dictyota coriacea (Homes) I.K. Hwang, H.S. Kim et W.J. Lee, comb. nov.

도로주행시험 평가 기반 인적 요인과 운전능력 간 관계 연구

황순천,이동민,홍일기 한국ITS학회 2019 한국ITS학회 학술대회 Vol.2019 No.04

독도 해조상과 계통지리학적 특성

김형섭,황일기,김선미,권천중 한국자연보호학회 2008 한국자연보호학회지 Vol.2 No.1

The species composition and phylogeographical features of marine algal flora of Dok island are investigated. A total of 253 species, 26 green, 67 brown, 160 red, were counted cumulatively since first report by Kang and Park (1969) from Dok island. Among them Ectocarpus corticulatus Saunder, Antithamnion okiense Kajimura are first reported from Korea and Feldmannia irregularis, F. globifera, Spatoglossum crassum newly added by this study. And also, a candidated new species, Dictyota sp. (Dictyotaceae, Phaeophyceae) is collected in this study. A remarkable increasing of warm water brown algal species, eg. Nemacystus decipiens, Sphaerotrichia divaricata, Tinocladia crassa, Petrospongium rugosum, Sargassum yezoense, are observed recently studies. This phenomenon is presumed to be closely related with global warming. For phylogeographical study of marine algae of Dok island we selected two species, one red alga Griffithsia japonica Okamura and one brown alga Dictyota coriacae (Holmes) Hwang, Kim et Lee, which have low dispersal capacity by their Oogamious sexual reproduction. The Dok island's haplotype of G. japonica is clearly distinguished from Oki island haplotype but closely related to the Korean type's haplotypes which distributed in south and east coast of Korea. And also, the Dok island's haplotype of D. coreacea is a unique Korean type which discriminated from Japanese and Taiwanese type. From these results, we proposed the marine algae of Dok island, at least in case of warm water species, were originated from Korean coasts rather than western coast of Japan by the Korean warm current.

국제소비자보호 세미나

안태호,일기황재 한국식품산업협회 1976 좋은식품 Vol.32 No.-

홍조류 지네지누아리와 뼈지누아리의 계절생장

이주일,황일기,박정광,김형근 한국자연보호학회 2011 한국자연보호학회지 Vol.5 No.1

Grateloupia asiatica and G. divaricata are edibile marine red algae that commonly occur on the east coast of Korea: G. asiatica occurs in sheltered locations such as estuaries or tide pools, and G. divaricata usually grows at the depth of 2~5 meters under the sea where freshwater flows. We compared growth pattern of both species in Aninjin, Gyoam, Hosan, and Sacheon in order to find suitable location for mass culture of the Grateloupia species. In case of G. asiatica, vegetative and tetrasporangial thalli were found in Gyoam and Sacheon, while gametophytic thalli were found in Aninjin. Population from Aninjin was the largest on the average length of thalli (20.6 cm) among populations studied. Population of G. divaricata in Sacheon seems to be maintained mostly with sporophytes. However, Aninjin and Hosan populations consisted of gametophytes and sporophytes in summer. Population from Hosan was the largest on the average length of thalli (19.8 cm). Based on our results, G. asiatica and G. divaricata repeat a typical triphasic life history of red algae on the east coast of Korea.

Diving PAM을 이용한 해초류와 해조류의 광합성 효율 비교

이상용,김영식,황일기,하동수,공용근 대한조선학회 2011 대한조선학회 학술대회자료집 Vol.2011 No.6

Reinstatement of Gracilariopsis chorda (Gracilariaceae, Rhodophyta) Based on Plastid rbcL and Mitochondrial cox1 Sequences

김명숙,양은찬,김수연,황일기,부성민 한국조류학회I 2008 ALGAE Vol.23 No.3

Two different molecular markers, the plastid rbcL and mitochondrial cox1 genes, were used to define the taxonomic position of the northwest Pacific Ocean species currently named Gracilaria chorda. We analyzed both genes (1,222 bp for rbcL and 1,245 bp for cox1) from 18 specimens collected in Korea, Japan, and China. Phylogenetic reconstruction revealed that this organism should be classified in the genus Gracilariopsis, rather than in the Gracilaria. Thus, Gracilariopsis chorda (Holmes) Ohmi is the legitimate name for Gracilaria chorda Holmes. Within the species, the sequences differed by 8 bp (0.7%) in rbcL and 5 bp (0.4%) in cox1. Six haplotypes of cox1 tended to be geographically organized. Gp. chorda is characterized by coarse, elongate terete axes, short filiform branchlets usually at irregular intervals, an abrupt transition in cell size from medulla to cortex, cystocarps without tubular nutritive cells connecting the gonimoblast to the upper pericarp, and relatively large gonimoblast cells of the cystocarp in the specimens collected from Wando in southern Korea. Two different molecular markers, the plastid rbcL and mitochondrial cox1 genes, were used to define the taxonomic position of the northwest Pacific Ocean species currently named Gracilaria chorda. We analyzed both genes (1,222 bp for rbcL and 1,245 bp for cox1) from 18 specimens collected in Korea, Japan, and China. Phylogenetic reconstruction revealed that this organism should be classified in the genus Gracilariopsis, rather than in the Gracilaria. Thus, Gracilariopsis chorda (Holmes) Ohmi is the legitimate name for Gracilaria chorda Holmes. Within the species, the sequences differed by 8 bp (0.7%) in rbcL and 5 bp (0.4%) in cox1. Six haplotypes of cox1 tended to be geographically organized. Gp. chorda is characterized by coarse, elongate terete axes, short filiform branchlets usually at irregular intervals, an abrupt transition in cell size from medulla to cortex, cystocarps without tubular nutritive cells connecting the gonimoblast to the upper pericarp, and relatively large gonimoblast cells of the cystocarp in the specimens collected from Wando in southern Korea.