진행성 위암 환자의 위조직에서의 p16 유전자의 메틸화와 돌연변이

장희경,김영옥,공은희,신연명,최경현 고신대학교(의대) 고신대학교 의과대학 학술지 2004 고신대학교 의과대학 학술지 Vol.19 No.1

Background/Aims : p16 has been suggested to be a tumor suppressor gene and to be inactivated in many human cancers. The aberrant expression of this gene proteins may deregulate cell proliferation and may lead to tumor formation and progression. The aim of this study was to evaluate the patterns of p16 inactivation and its association with clinicopathological parameters in 16 cases of advanced gastric cancers having fresh and paraffin-embedded tissues. Material and Methods : p16 inactivation by immunohistochemistry, mutation by direct sequencing and methylation analysis by methylation-specific PCR in exon 1 and 2 in 16 cases of advanced gastric cancers. Results : Eight of 16 (50.0%) carcinoma tissues and 3 cases (18.8%) of non-neoplastic gastric tissues showed loss (inactivation) of p16 and 5 (31.3%) methylations, and 3 cases of non-tumor gastric mucosa showed 1 mutation (6.3%) and 2 methylations (12.5%). Interestingly, one of 3 mutations showed also same mutation in adjacent non-neoplastic tissue, while 2 methylations were identified in non-neoplastic mucosal tissues without methylation in corresponding cancer tissues. The parameters histologic types and differentiation of tumors, sex, age, invasion depth, and vascular invasion did not correlate with p16 gene alteration, while nodal metastasis and perineural invasion showed positive correlation. Conclusions : These results suggest that p16 inactivation may have effects on the carcinogenesis of gastric mucosa and may serve as a prognostic biomarkers for the progression of carcinoma.

니트 조직별 패턴의 맞음새 비교연구

장희경,윤혜준,안재상,김영숙 한국니트디자인학회 2014 패션과 니트 Vol.12 No.2

In this study, the knit is different depending on the degree of expansion, taking into account the characteristics of knitted fabrics and knitted apparel products to the most commonly used patch Plain knit, 0×0 Rib knit, 2×1 Rib knit considering the characteristics of the pattern design method according to the pullover knitwear to suggest the study. The design of the experiment, this experiment pullover knitwear garment sleeve knit organization in accordance with the form's overall silhouette and a laboratory coat armhole seams and around the area of the wrist to determine the difference between wearing a round neck line by selecting a basic sleeve design Plain knit, 0×0 Rib knit, 2×1 Rib knit was made​​. Wearing standard figure for experiment 20 subjects were selected to correspond to the physical measurement is performed, according to the organization's knitwear for a comparative analysis of patterns and silhouettes look twice using the method for sensory evaluation were evaluated Dressing.Modify the physical description of the second experiment, complemented by an organization patternmaking pullover knitwear patterns are presented. In this study, a higher rate of production in the clothing company's knitwear production in the largest stretch knit with organization-specific, reflecting the characteristics of knitwear patterns according to silhouettes and contribute to the utilization.

15년 전의 신의 투명 세포암에 속발하여 발생한 갑상선의 투명 세포암에 대한 조직화학 및 면역병리학적 검색 : A case report HIstochemical and Immunohistochemical Studies

장희경,김혜숙,허방,허만하 高神大學 醫學部 1988 高神大學醫學部 論文集 Vol.4 No.1

영남지방 돼지의 Bordetella bronchiseptica 감염상황 및 분리균의 생화학적 특성

장희경,김봉환,Jang, Hee-kyung,Kim, Bong Hwan 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1

The present study was conducted to investigate the Bordetella bronchiseptica infection in Youngnam swine herds during the period from August 1986 to July 1987 and some properties of the organisms isolated from these Korean swine. B. bronchiseptica was recovered from 25 of 70 (35.7%) glowing pigs of 4 to 10 weeks of age and from 12 of 13 (92.3%) herds. From 115 slaughter pigs, 58(50.4%) pigs were culture positive and the pigs from 13 of 14 (92.9%) herds were found to be infected with B. bronchiseptica. The majority of biochemical and cultural properties of B. bronchiseptica isolated from Korean swine were identical to those of the standard strain employed and some 97.6% of the isolates showed the characters of phase I organism on primary isolation.

영남지방 돼지에서 분리한 Bordetella bronchiseptica의 약제 감수성

장희경,김봉환,Jang, Hee-kyung,Kim, Bong Hwan 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1

The in vitro drug susceptibility of 83 strains of Bordetella bronchiseptica recovered from Korean pigs with atrophic rhinitis was investigated by the use of disk diffusion method. The majority of the organisms were highly resistant in order of prevalence to penicillin(98.7%), ampicillin(91.5%), streptomycin(90.3%), triple sulfa(83.1%), and trimethoprim/sulfamethoxazole(70.7%) while none of them were resistant to gentamicin, only 3.6% to colistin, chloramphenicol and kanamycin and 6.0% to tetracycline. The percentage of the organism resistant to bicozamycin, cephalothin and neomycin were 34.9%, 34.1% and 18.4%, respectively. A high prevalence of multiple drug resistance was observed and the 3 most common resistant patterns among 35 patterns noted were Am Pc Sm Sss Sxt(26.5%), Am Cf Pc Sm Sss Sxt(12.%) and Am Bm Pc Sm Sss Sxt(9.6%) patterns.

15년 전의 신의 투명 세포암에 속발하여 발생한 갑상선의 투명 세포암에 대한 조직화학 및 면역병리학적 검색

장희경,김혜숙,허방,허만하 고신대학교(의대) 고신대학교 의과대학 학술지 1988 고신대학교 의과대학 학술지 Vol.4 No.1

A case of metastatic clear cell carcinoma of the thyroid in a 60 year old Korean male, ensuing clear cell carcinoma of the kidney after 15 years, is reported, associated with a literature review, in view of its rarity and the method of surgicopathological access we adopted toward the final anatomic diagnosis. An emphasis is placed on the way of differential diagnosis of metastatic carcinoma from primary clear cell carcinoma of the thyroid andn secondary malignancy from primary parathyroid carcinoma. Not only gross and ordinary microscopic observations including special stains, and immunoperoxidase method is performed to confirm that the thyroid lesion is not of the thyroid cell origin, which reconfirms the immunopathological method is a useful tool in surgical pathology.

장형 위암에서,r公2, c-MYC,beta-catenin, APC 단백을 포함한 APC유전자경로의 역할

장희경 고신대학교(의대) 고신대학교 의과대학 학술지 2006 고신대학교 의과대학 학술지 Vol.21 No.1

Background: A proposal that in normal colorectal epithelial cells,the adenomatous polyposis coli gene (APC) might modulate directly c-MYC transcription through beta-catenin/Tcf-4 was reported in 1998. It was reported that intestinal trefoil factor (TTF) which is the same family of pS as a gastric - specific tumor suppressor gene is related to beta-catenin. Method and Material: To elucidate the applicability of APC pathway in stomach and relationship between this pathway and pS2, the expressions of pS2,c-MYC, and beta-catenin proteins wereevaluated by immunohistochemically in 30 cases of intestinal carcinoma of stomach which is morphologically close to colon cancer Restults; Their immunoreactivities were 5公7% for pS2,43.3% for c-MYC, and 86,7% for beta-catenin proteins, respectively. Interestingly, positive staining of beta-catenin showed heterogenous pattern according to the depth of invasion. Tumor cells in the invading or infiltrating edges showed strong positivity,while thetumor cells in mucosa showed loss of the expression even ib the same tumors. Conclusion: This results suggest that in intestinal carcinoma of stomach, beta-catenin involves in the tumor cell migration and invasion not as cell adhesion molecule but as tumor-suppressor molecule forming complex with APC. In addition, the expressions of c-MYC and pS2 was not correlated with the function of adhesion molecule of beta-catenin

급성 뇌졸중 입원 환자의 연하장애 유병률과 관련요인

장희경,윤숙경,길초롱 국제문화기술진흥원 2019 The Journal of the Convergence on Culture Technolo Vol.5 No.1

The aims of this study were to evaluate the prevalence of dysphagia in patients hospitalized with acute stroke and to identify factors associated with dysphagia. The study was conducted in a tertiary hospital and a total of 131 patients hospitalized with acute stroke (aged 34-92 years old, 58.0% male) were enrolled. The prevalence of dysphagia was 24.4% out of 131 participants and risk factors associated with dysphagia were age, neurological severity, oral health status, and dependence in activity of daily living of participants. Considering the long-term rehabilitation trajectory, it is necessary to apply the early screening and preventive nursing intervention for dysphagia during acute stage of stroke patient. 본 연구는 급성 뇌졸중 입원 환자의 연하장애 유병률과 그 관련요인을 탐색하기 위한 서술적 조사연구로, 일상급종합병원에 급성 뇌졸중으로 진단받은 입원 환자 131명(연령 범위 34-92세, 58.0% 남성)을 대상으로 수행되었다. 본 연구의 결과 급성 뇌졸중 입원 환자의 유병률은 24.4%였으며, 대상자의 나이, 뇌졸중 중증도, 구강상태, 일상활동의존도가 연하장애에 영향을 미치는 것으로 나타났다. 이러한 결과를 기반으로 뇌졸중 발병 직후부터 연하장애 조기발견을 위한 적극적인 간호사정과 예방적 중재 프로그램 개발과 적용이 필요하다.

한국판 간호사-환자 상호작용 측정도구의 신뢰도 및 타당도 검증

장희경,이지연,김미경,양은옥,길초롱 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.12

This study examined the reliability and validity of the Korean version of the Nurse-Patient Interaction Scale (K-NPIS) for the elderly, at long-term care hospitals in Korea. The original NPIS was developed to identify important characteristics of elderly patient experiences regarding nurse-patient interactions in nursing homes. Totally, 202 hospitalized elderly patients at 4 long-term hospitals in Jinju-si, Suncheon-si, and Namwon-si, were enrolled in the study and completed the questionnaire. For internal consistency, Cronbach's α of K-NPIS was 0.96 after item analysis. K-NPIS was validated using the confirmatory factor analysis and content validity. The content validity score was more than 0.80, establishing the appropriateness and readiness of the tool through group discussions between clinical experts and elderly patients. The K-NPIS is a 10-point scale comprising 12 questions. K-NPIS scores between different residential groups were significantly different, and showed no ceiling or floor effect. The internal consistency reliability, construct validity, and discriminative validity of K-NPIS was adequate. However, criterion validity requires further examination. Our findings suggest that K-NPIS serves as an appropriate measure of nurse-patient interaction when applying the nursing care intervention for elderly 본 연구는 노인환자가 인식하는 간호사와의 상호작용을 측정하기 위해 개발된 the Korean version of Nurse-Patient Interaction Scale (K-NPIS)을 한국 요양병원에 입원한 노인환자를 대상으로 간호사와의 상호작용을 측정의 신뢰도와 타당도를 검증하려는 목적으로 시행되었다. 진주시, 순천시, 남원시에 소재하는 4개의 요양병원에 입원한 노인환자 202명을 대상으로 신뢰도와 타당도 검증을 실시하였다. 문항분석과 내적일관성 검증을 통해 K-NPIS의 Cronbach α 값은 .96이었다. 전문가들을 통한 내용타당도 검증에서 내용타당도 지수가 0.8 이상으로 확인되었으며, 확인적 요인분석을 거쳐 최종 단일속성으로 구성된 12개 문항 각 10점 척도의 최종 도구가 도출되었으며, 재원기간에 따라 K-NPIS의 점수가 유의하게 차이가 나(p=.042) 판별타당도도 검증되었고, 천장효과나 바닥효과는 나타나지 않았다. 이와 같이 K-NPIS는 신뢰도와 타당도가 높은 도구임이 검증되어 요양병원에서 노인환자를 대상으로 간호사-환자의 상호작용을 측정하는 도구로 사용되는데 적절한 것으로 보이나 장차 준거타당도를 검증할 필요가 있다. K-NPIS는 노인환자가 인식하고 있는 간호사와의 상호작용을 자가보고 형식으로 측정하는 것이므로, 노인환자와의 상호작용을 기반으로 한 돌봄 중재의 성과를 평가하여 간호서비스 질 향상에 기여할 수 있을 것으로 기대된다.

미·중 경쟁 시대 독일의 지정학적 헤징 전략: 일대일로 vs. 인도-태평양

장희경 고려대학교 일민국제관계연구원 2023 국제관계연구 Vol.28 No.2

이 글은 미·중 경쟁 시대에 중견국으로서 독일의 지정학적 전략을 헤징 개념에 기반해서 분석한다. 미·중 경쟁 시대에 양 국가 사이에 끼인 국가들의 전략은 주로 아시아에 집중되어 왔다. 그러나 유럽, 그중에 독일도 안보는 미국, 경제는 중국에 의존하는 상태에서 미·중 경쟁에 따른 불확실성과 위기를 관리해야 할 필요가 있다. 강대국의 경쟁 구조에서 불확실성과 위기를 관리하는 전략으로서 헤징 개념은 다양한 전략적 행위를 포함한다. 지금까지 독일의 정책은 ‘회색지대’라는 표현처럼 모호성으로만 분석되어 왔지만, 독일도 국가의 이익에 따라 다양한 헤징 전략을 취하는 것으로 보인다. 특히 독일이 헤징 전략을 선택할 때 판단의 근거는 ‘다자주의’, ‘규범에 기반한 국제질서’라는 원칙과 규범이라고 본다. 이를 헤징의 다양한 전략 중 연성 균형의 한 형태로서 ‘제도적 균형’으로 설명하고자 한다. 사례연구로는 지정학적 미·중 경쟁의 대표 사례인 중국의 일대일로와 미국의 인도-태평양 전략에 대한 독일의 전략을 헤징 개념으로 분석한다. This article analyzes Germany’s geopolitical strategy as a middle power in the era of US-China competition based on the concept of hedging. In the era of US-China rivalry, the strategies of countries caught between the two countries have been mainly focused on Asia. However, Europe, especially Germany, needs to manage the uncertainties and crises caused by the US-China rivalry while relying on the US for security and China for economy. The concept of hedging as a strategy for managing uncertainty and risk in the context of great power competition encompasses a range of strategic behaviors. So far, Germany’s policy has been analyzed only in terms of ambiguity, such as the expression “gray zone,” but it seems that Germany also adopts various hedging strategies according to its national interests. In particular, the principles and norms of ‘multilateralism’ and ‘norms-based international order’ seem to be the basis for Germany's judgment when choosing a hedging strategy. It will explain this as ‘institutional balance’ as a form of soft balance among the various strategies of hedging. As a case study, this article analyze Germany’s strategy for China’s Belt and Road and the United States’ Indo-Pacific strategy, which are representative cases of geopolitical U.S.-China competition, using the concept of hedging.