청고추, 쇠비름, 표고버섯의 물 추출물 및 당침액의 항산화 활성 및 ${\alpha}$-Glucosidase Inhibition 활성 비교

이성미,강윤환,김대중,김경곤,임준구,김태우,최면,Lee, Sung Mee,Kang, Yun Hwan,Kim, Dae Jung,Kim, Kyoung Kon,Lim, Jun Gu,Kim, Tae Woo,Choe, Myeon 동아시아식생활학회 2014 동아시아식생활학회지 Vol.24 No.1

본 연구에서는 최근 건강 관리를 위해 다양하게 상용되고 있는 당침액의 오 남용을 막기 위해 과학적으로 물 추출물과 당침액의 특성을 규명하고자 하였다. 그 결과, 일반 물 추출물과 비교하였을 때 당침액의 총 폴리페놀 및 플라보노이드 함량이 크게 감소하는 것을 확인하였으며, 그에 따라 항산화 활성 또한 감소한 것을 확인할 수 있었다. 이는 물 추출물과 비교하여 당침액의 건조 분말에는 설탕 또는 그 분해물들이 많은 부분을 차지하기 때문이라 판단된다. 그러나 혈당강하 효능의 target 효소로 연구되고 있는 ${\alpha}$-amylase 및 ${\alpha}$-glucosidase의 활성을 측정한 결과, 청고추, 쇠비름, 표고버섯의 물 추출물과 당침액은 ${\alpha}$-amylase 억제 활성을 나타내지 않았지만, ${\alpha}$-glucosidase 억제 활성은 의약품인 acarbose에는 미치지 못하였으나, 물 추출물에 비해 당침액이 농도 의존적으로 높은 억제 활성을 나타내었다. 또한 세포 독성을 HepG2 cell에서 확인한 결과, 물 추출물과 비교하여 당침액의 독성이 약 1/10 감소한 것을 확인할 수 있었다. 이와 같은 결과는 민간에서의 믿음처럼 당침액이 물 추출물과 비교하였을 때 모든 부분에서 우수한 기능을 가진다고 할 수 없으며, 활용할 용도에 따라 소재를 물 추출하거나 당침액을 제조하는 등의 가공 과정을 거치는 것이 목표한 기능을 효과적으로 확보하는 방법임을 의미한다. 따라서 민간에서 맹목적으로 믿는 당침액의 효능에 대한 과학적인 연구 접근법은 당침액의 적절한 사용 목적과 방법을 설정하는 기초 자료로 활용이 가능할 것이다. This study was conducted in order to compare the biological activities of water extracts and sugar immersion extracts of green pepper (Capsicum annuum L.), purslane (Portulaca oleracea L.) and shiitake (Lentinula edodes (Berk.) Pegler) by measuring total polyphenol and flavonoid contents, antioxidant activities and inhibitory effects on ${\alpha}$-amylase and ${\alpha}$-glucosidase. The contents of total polyphenols and flavonoids were higher in water extracts than in sugar immersion extracts. The anti-oxidative activities of water and sugar immersion extracts were measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity assay and reducing power assay. All extracts scavenged radicals in a concentration-dependent manner, and water extracts showed stronger radical scavenging activity and reducing power than sugar immersion extract. However, they all exhibited lower activities than ascorbic acid. Compared to the anti-diabetic drug acarbose, which was used as a positive control, the two types of extracts exhibited low ${\alpha}$-glucosidase inhibitory activities, although the activity of sugar immersion extracts were 2-fold higher than that of water extracts. ${\alpha}$-Amylase inhibitory action was not observed for any of the extracts. Finally, by cytotoxicity test, we confirmed that sugar immersion extracts were safer than water extracts. These results indicate that water extracts and sugar immersion extracts of green pepper, purslane and shiitake have different advantages in terms of their antioxidant and anti-diabetic effects, respectively.

Tetracycline 처리된 조직유도재생술용 차폐막의 약제유리양상 및 구조적 변화

이성미,정현주,Lee, Sung-Mi,Chung, Hyun-Ju 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.1

Tetracycline is known to be effective in eliminating periodontopathogens and have collagenolytic activity. This study was performed to observe the desorption kinetics and structural changes of tetracycline-treated barrier membranes for guided tissue regeneration. Four kinds of barrier membranes were tested : $Tefgen^{(R)}$(American Custom Medical, USA) and $Gore-Tex^{(R)}$(W.L. Gore & Associates Inc., USA) as nonresorbable membranes ; Resolut(polyglycolide & polylactide copolymer, W.L. Gore & Associates Inc., USA) and $Biomend^{(R)}$(collagen, Collatec Co., USA) as resorbable membranes. The membranes were cut into discs(diameter : 4mm) and were immersed in 5% tridodecylmethylammonium chloride(TIMAC) ethanol and air-dried. The membrane discs were absorbed with $100{\mu}g/ml tetracycline solution(pH8) for one minute and dried. For desorption kinetics, TC treated discs were immersed in phosphate buffered saline solution (PBS, pH 7.4). PBS was exchanged daily and TC concentration was measured by absorbance at 276nm on UV spectrophotometer. To measure remaining antibacterial activity, discs of 1 day to 4 weeks after desorption were placed on Mueller Hinton agar containing Bacillus cereus and incubated aerobically in $37^{\circ}C$ for twelve hours and the inhibition diameters were measured. To observe the structural change of membranes after TIMAC treatment or immersion in PBS, the membrane discs were examined under SEM. The results were as follows : 1. Total amounts of TC absorbed into membrane discs($0.7536mm^2$) were $2000{\mu}g$, $1800{\mu}g$, $2625{\mu}g$ and $2499{\mu}g$ for $Tefgen^{(R)}$, $Gore-Tex^{(R)}$, $Biomend^{(R)}$ and $Resolut^{(R)}$. 2. The concentration of TC released from barrier membrane discs was maintained over $4{\mu}g/ml$ until the fifth day in nonresorbable membranes and $Resolut^{(R)}$, but until the fourth day in $Biomend^{(R)}$, Until the ninth day in nonresorbable membranes and until the seventh day in resorbable membranes, the TC concentration was maintained over $1{mu}g/ml$. 3. The four membrane discs in the first day showed similar size of inhibition zone. One to four weeks later, the inhibition zone was much smaller in resorbable membrane discs than nonresorbable membrane discs. 4. Any structural change due to treatment of TIMAC was not observed on the nonresorbable membranes. $Resolut^{(R)}$ did not show any structural change except fibrillar loosening during immersion period, but Biomend showed destruction of membrane structure from the first week of immersion. This study indicates that tetracycline treated barrier membranes lead to the sustained release of tetracycline for over 7 days. This slow release pattern of tetracycline may contribute to the favorable clinical outcome of guided tissue regeneration.

보건관리대행사례47 - 제조업의 뇌심혈관질환 예방 사례

이성미,Lee, Seong-Mi 대한산업보건협회 2009 산업보건 Vol.250 No.-

주요 신문매체를 통해 본 아파트 주거문화 분석

이성미,이연숙,Lee Sung-Mi,Lee Yeon-Sook 한국주거학회 2005 한국주거학회 논문집 Vol.16 No.3

Daily newspapers are one of the most influential mass media and the news coverage can be used an indicator of social issues and needs. The purpose of this study was to investigate characteristics of physical environment in apartments, and to examine the underlying social ideas. In doing so, the contents of national daily newspapers in the past ten years were analyzed by using frequencies. The study found that sophistication, diversity, and differentiation were drawn as social values that lie in the physical environment of apartments, and the characteristics were related to housing types, space planning and complex layout. The finding shows that the coverage of the physical environment in apartments has been growing, and the increasing attention to the residential environment remains strong and significant.

여성결혼이민자 가족의 사회통합 지원대책

이성미,Lee, Seong-Mi 충남발전연구원 2006 열린충남 Vol.35 No.-

노인주택의 개념과 유형화 연구

이연숙(Lee, Yeunsook),이성미(Lee, Sungmi),김민수(Kim, Minsoo),이유진(Lee, Yoojin),이선민(Lee, Sunmin) 한국생태환경건축학회 2007 한국생태환경건축학회 논문집 Vol.7 No.1

차례 : 사회생물학적 관점에서 본 부모의 욕구

이성미 ( Seong Mi Lee ) 한국교육철학회 2012 교육철학 Vol.46 No.-

Many modern parent-theories tacitly premise norms that parent-child relationship should be full of love, understanding, encouraging, and comforting. Even though they regard the conflicts between parent and child as their subject of the study, it is very difficult to find their basic questions such as why and from where the conflicts begins, Besides, even though they present how to be good parents, they do not show radical skepticism about ``what is the real parent-child relationship``. In fact, parental love for child, on which parental authority and responsibility are based, has been accepted as indemonstrable theme so far. But we need to ascertain the realities or facts before discussing about parental sacrifice, love, authority, and responsibility. This article focuses on the parent`s desires among many facts which explain parent-child relationship. Because parent`s desires based on his/her power cannot avoid the conflict with child`s independent freedom, it becomes the key reason that causes complications between parent and child. As the first step of philosophical thinking about parent-child relationship, with priority given to parent`ffs desires, I tried to infer parent`ffs biological desires from some sociobiological researches which analyze the cause of altruistic instinct. The most of relationships that we can find purely altruistic instinct are the ones between parent and child. Sociobiology finds the reason from gene, which has the nature of self-reproduction as the only survival principle. Through the child who inherits his/her gene, parent feels the life which survives transcending the limits of generations. On the other hand, parent has the desire to recreate him/herself through his/her child because of the self-centered and out-intentional consciousness. But child is not an extension of parent him/herself but an extension of the immortal life in parent. Child is another independent existence and the fate of parent is to help child become wholly independent from parent.

공간디자인의 감성에 대한 개념적 연구

이성미(Lee, Sungmi) 한국실내디자인학회 2008 한국실내디자인학회논문집 Vol.17 No.1

브랜드 인게이지먼트에 브랜드 앱이 미치는 영향에 관한 실증연구

이성미 ( Lee Sung-mi ) 커뮤니케이션디자인학회(구 시각디자인학회) 2018 커뮤니케이션 디자인학연구 Vol.63 No.-

브랜드 인게이지먼트 강화는 수많은 마케팅 콘텐츠가 난무하는 디지털 시대에 브랜드의 충성고객을 확보하기 위한 중요한 과제이다. 본 연구는 브랜드 인게이지먼트 개념을 보다 정확하게 이해하기 위해 전통적으로 교육학에서 연구되어왔던 인게이지먼트 이론의 3가지 측면(정서적, 인지적, 행동적)살펴보았고 기대일치이론을 통해 브랜드 앱에 대한 기대일치 수준 및 만족도와 브랜드 인게이지먼트의 관계에 대해 논의하였다. 연구 결과에 따르면 앱의 유용성과 앱의 기대일치 수준은 브랜드 인게이지먼트에 긍정적으로 영향을 주었고 앱에 대한 만족도는 앱의 유용한 가치 및 기대일치 수준과 브랜드 인게이지먼트 간의 관계를 조절하였다. 본 연구는 교육학의 전통 인게이지먼트 이론을 적용하여 향후 다양한 브랜드 인게이지먼트 관련 연구를 할 수 있는 학문적 기반을 마련하였다. 또한, 브랜드 앱을 소비자와 커뮤니케이션 목적으로 디자인 할 때 브랜드 앱을 통해 소비자와 정서적으로 소통하고 소비자의 인지양식에 영향을 주고 소비자의 특정 행동에 동기를 부여하기 위한 전략적 기반을 제공한다. This research explores the relationship between perceived value of branded applications(apps) and brand engagement. Also, this research investigated how consumer satisfaction with branded apps moderate the relationship between perceived value of apps and brand engagement. The findings of survey show that the utility of apps and the level of confirmed expectation are significant factors in predicting brand engagement. Also the results confirm that consumer satisfaction with branded apps moderates the relationship between perceived value of apps and brand engagement. Important implications of the study are discussed.

긍정적 청소년 개발 개념에 근거한 청소년 여가시설의 개선점 연구

이성미 ( Sung Mi Lee ) 한국공간디자인학회 2016 한국공간디자인학회논문집 Vol.11 No.1

(연구배경 및 목적)청소년기는 아동에서 성인으로 이행되는 매우 중요한 시기로서 육체적 성숙 뿐만 아니라 자아정체성이나 사회성, 가치관 등이 형성되는 매우 중요한 시기이다. 그런데 우리나라의 청소년들은 학업이나 입시위주의 교육환경으로 인해균형 있는 성장의 기회를 충분히 가지지 못하고 있다. 청소년기의 가치관이나 도덕성, 직업역량 등의 적극적인 개발은 국가의 미래가 됨에도 불구하고 사회적으로 제공되는 청소년 개발 프로그램은 문제 중심이나 예방적 차원을 벗어나지 못하고 있다. 긍정적인 관점에서의 청소년 개발이란 청소년을 무한한 발전가능성을 가진 존재로 보고, 청소년들에게 다양한 체험이나 활동 등을할 수 있는 기회를 제공함으로서 성인으로 성장하는데 필요한 신체적 정서적 인지적, 사회적, 시민적, 직업적 측면에서의 역량들을 발달시켜 나가는 것이다. 이러한 역량 개발은 청소년의 자발적이고 비구조적이며 지속적인 여가활동에 의하여 가능하며, 이를 위해 사회는 청소년들을 위해 안전하고, 다양하며, 지속적인 발달을 도울 수 있는 청소년 전용의 여가환경을 개발하고 제공할필요가 있다. (연구방법)본 연구에서는 청소년의 긍정적 발달 개념에 대하여 알아보고 이러한 발달을 돕기 위한 청소년의 여가활동과 여가환경의 특성에 대한 요구를 문헌고찰을 통해 알아보았다. 또한 청소년들의 여가문화와 여가시설에 대한 선행연구를 통해 종류 및 현황, 문제점을 고찰하였다. 또한 긍정적 청소년 발달을 위해서는 생활권의 여가시설이 더욱 중요하다고 보고 생활권 여가시설의 현황과 개선점을 알아보기 위해 사례조사를 하였다. (결과)청소년 여가시설로는 청소년 활동진흥법에 근거한 수련시설과 이용시설이 있으며 위치에 따라 자연권과 생활권으로 분류할 수 있다. 청소년여가시설은 대부분 자연권에 주로 분포되어 있어 접근성이 떨어지고 시설수도 부족하다. 생활권의 여가시설인 청소년 문화의 집은 프로그램이 한정되어 있어 다양한 청소년 여가활동 욕구를 채워 줄 수가 없으며 청소년에게 인지도가 낮아 이용도가 떨어진다. 특히 장비나 공간의 크기, 수, 설비 등의 개선이 요구되고 있다. 청소년 여가시설은 접근성(생활권/자연권), 운영주체(공설/사설), 경제성(무료/유료, 상업적/비상업적), 서비스(개별형/연계형/복합형), 프로그램(일회형/지속형), 활동목적(휴식및놀이형/사회문화활동형/학습및자기계발형) 참여특성(구조적/비구조적) 등의 기준을 검토하여 다양하고도 합목적적인 제공이 이루어지도록 요구된다. (결론)청소년의 긍정적발달을 위한 여가문화의 특성 연구가 필요하다. 청소년들의 실제적인 여가환경 요구도에 대한 심층적인 조사가 필요하며, 생활권의 청소년 여가시설의 개발, 운영, 연계에 대한 다각적인 연구를 통해 청소년의 여가문화를 질적으로 개선할 수 있는 방안이 요구된다. (Background and Purpose)Adolescence is a very important period for transition from children to adults, wherein not only physical maturation takes place, but self-identity, sociability, and values are developed. However, youth in South Korea do not have adequate opportunities for balanced growth due to learning or entrance examination oriented educational environment. Even though active development of values, morality, and vocational competence in adolescence enriches the future of the country, socially supported youth programs are focused on only problem oriented or preventive dimensions. Youth development means considering youth as beings that have infinite possibility for development, and providing opportunities for diverse experiences or activities to develop their physical, emotional, cognitive, social, civil, and vocational capabilities necessary for them to grow into adults. These capabilities can be developed through youths`` voluntary, unstructured, and incessant leisure activities. To this end, society should develop and provide safe and diverse leisure environments dedicated to youth that can facilitate their continuous development. (Method) In the present study, concept of positive development of youth, requirements for youths`` leisure activities, and leisure environments necessary to facilitate such development were examined through literature review. In addition, the kinds, present situation, and problems of youths`` leisure cultures and leisure facilities were contemplated through review of previous studies. In addition, presuming that leisure facilities in living zones are more important for positive development of youth, case studies were conducted to examine the present situation and areas of improvement in leisure facilities in living zones. (Results) Youths`` leisure facilities include training facilities and use facilities based on the Juvenile Activity Promotion Act and their locations can be divided into natural zones and living zones. Youths`` leisure facilities are mostly distributed in natural zones with poor accessibility and the number of such facilities is not sufficient. Youth Cultural Centers, which are leisure facilities in living zones, cannot satisfy youths`` demands for diverse leisure activities because of their limited programs, and these centers are not frequently used because awareness of these centers among youths is low. In particular, they need to be improved with respect to their size, number of equipment, space, and facilities provided. Youths`` leisure facilities should be diversely available and serve their purpose through review of standards for accessibility (living zone/natural zone), operators (public/private), economic feasibility (free/charged, commercial/non-commercial), services (individual/linked/composite), programs (one-shot/continuous), activity purposes (rest and play/social or cultural activities/learning and self-development), and participation characteristics (structured/unstructured). (Conclusions) The characteristics of leisure cultures should be studied for positive development of youth. Youths`` needs of actual leisure environments should be examined comprehensively and measures to qualitatively improve youths`` leisure cultures should be developed through multilateral studies of development, operation, and nexus of youths`` leisure facilities in living zones.