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키랄컬럼 쌍을 이용한 레보도파 제제 중의 l-도파와 d-도파의 HPLC 분석
주홍매,오은기,진건파,주반멘,이은주,이은실,강종성 충남대학교 약학대학 의약품개발연구소 2010 藥學論文集 Vol.25 No.-
Levodopa preparations are used for the treatment of Parkinson's disease, since it is capable of crossing the protective blood-brain barrier (BBB), whereas dopamine itself cannot. d-Dopa could be often contained to a pharmaceutical levodopa preparations as impurity, however, the Korean Pharmacopeia doesn't regulate the amount of d-dopa in levodopa preparations. A new, precise and simple HPLC method for determination of l-dopa and d-dopa from levodopa formulation was developed using a pair of chiral columns, SCA and RCA, for the purpose of quality control. The selected mobile phase was composed of methanol and water (80 : 20) containing 0.01% phosphoric acid. The flow rate was 1.0 mL/min and detection wavelength was 210 nm. The intraday precision of l-dopa and d-dopa analysed by developed method were 2.71~8.18% and 2.94~6.03%, respectively. Both dopa enantiomers were separated by SCA and RCA column, a pair of chiral columns. However, RCA column was useful for the analysis of l-dopa, while SCA column for d-dopa. The method was validated according to ICH Guidelines and the method was found to be simple, accurate, precise, economical and robust.
박기주,이은실,오은기,이은주,황귀서,신윤상,강종성 충남대학교 약학대학 의약품개발연구소 2008 藥學論文集 Vol.23 No.-
Atractylodes japonica Koidzumi, A. macrocephala Koidzumi and A. lancea D.C. are the main sources of Atractylodis Rhizome(Alba). The chromatographic pattern was evaluated as a method of quality evaluation by HPLC and TLC. The main evaluation criteria were arithmetic mean, weighted mean, similarity and matching ratio. The concentration of the components in fresh roots was higher than that of in preserved roots of A. japonica and A. macrocephala. The wild species showed higher concentration than the cultivated species of A. japonica. The smilarity of Atractylodis Rhizoma(A lancea) was 20%, while that of Atractylodis Rhizoma Alba(A. japonica, A. macrocephala) was higher than 55%, indicating that Atractylodis Rhizoma Albaand Atractylodis Rhizoma could be differentiated by this method.
최현수,진건파,오은기,이은주,이은실,박경래,강종성 충남대학교 약학대학 의약품개발연구소 2009 藥學論文集 Vol.24 No.-
Chirality plays an important role in biology, and many of the processes essential for life are stereospecific. Hence, the chiral chromatographic separation of drugs becomes more important for the pharmacologists and toxicologists to offer the appropriate tools to find the solution for many problems related with stereoisomers of drugs. This review discusses the clinical applications of chiral separation for meploquine salbutamol, omeprazole, ketamine and some fluorinated volatile inhalation anaesthetics. The chirality in clinical situations was illustrated by these drugs in the field of enantiospecific pharmacokinetics and therapeutic activities. The results indicated that the racemic switch to a single enantiomer for these drugs will promote the development of more effective and safe drugs.
강종성,황귀서,이현선,박기주,오은기,이은실,김영호 한국생약학회 2008 생약학회지 Vol.39 No.3
Three species, such as Rheum palmatum L., R. tanguticum Maxim. and R. officinale Baillon are recognized as the source plants of Rhei Rhizoma in Korean Pharmacopeia. However, other herbal sources such as R. undulatum L. and Rumex crispus L. have been often misused as Rhei Rhizoma. A pattern analysis method to discriminate Rhei Rhizoma in Korean Pharmacopeia from other herbal plants using HPLC and TLC chromatograms was developed. The multivariate peak data of the chromatograms of methanol extracts of Rhei Rhizoma were used for hierarchical clustering analysis, principal components analysis and similarity calculation. Besides of the statistic analysis, TLC patterns of samples could be used as criteria of the discrimination. The developed pattern analysis method was specific and could be readily utilized for comprehensive evaluation of Rhei Rhizoma.