백서와 기니픽의 대뇌피질에서 Opioid Kappa 수용체의 특성에 관한 연구

김기원(Kee-Won Kim),노혜원(Hye-Won Rho),김형일(Hyoung-Il Kim),은재순(Jae-Soon Eun),소수미(Soo-Mi Soh),조규박(Kyu-Park Cho) 대한약리학회 1994 대한약리학잡지 Vol.30 No.2

In this study, we tested the influences of several κ opioid ligands on the [³H]diprenorphine binding in rat and guinea pig cortex membrane preparations. Using paradigm to block μ and δ opioid receptors with DAMGO(1μM) and DPDPE(1μM), [³H]diprenorphine labeled κ sites. Competition analysis in both rat and guinea pig cortex has shown a single population of [³H]diprenorphine binding site with different Kd values, respectively. There is a significant difference in Ki values of (-) WIN44441 and (+)WIN44441 in both rat and guinea pig cortex. Bremazocine, (-)ethylketocyclazocine, (-)cyclazocine, nor-binaltorphimine effectively inhibited the [³H]diprenorphine binding with different Ki values in rat and guinea pig cortex. U-69,593, U-50,488H and dynorphine-A (1-8) did not inhibit the [³H]diprenorphine binding in rat but in guinea pig cortex. Nor-binaltorphimine was a ligand discriminate the κ₁, and κ₂ receptor most effectively. We, also, examined the influence of Na ion and GTPγS, a nonhydrolyzable guanine nucleotide analog, on the inhibition of [³H]diprenorphine binding by diprenorphine, (-)ethyl-ketocyclazocine, U-69,593 and bremazocine. By the replacement of NaCl with N-methy-D-glucamine or addition of GTPγS, Ki values of diprenorpnine were not changed and that of ethylketocyclazocine were changed significantly in both rat and guinea pig cortex. The Ki value of bremazocine was decreased by removal of Na ion, and increased by GTPγS, however, was not changed by any one of either. These results suggest that there are 2 kinds of subtypes of κ opioid receptor, κ₁, and κ₂, showing different Ki values for various κ opioid ligands, also, bremazocine possess the antagonistic property at κ₂ site which is dominant subtype of K receptor in rat cortex.

백서 대뇌피질절편에서 산소 및 포도당 결핍에 의한 [³H]Acetylcholine의 유리에 관한 연구

이정주,김기원,서만욱,김영현,김기원 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

권역심뇌혈관질환센터 사업의 비용-편익 분석

김병호 ( Byung Ho Kim ),감신 ( Sin Kam ),배상근 ( Sang Geun Bae ),김건엽 ( Keon-yeop Kim ),이원기 ( Won Kee Lee ),홍남수 ( Nam-soo Hong ),박형근 ( Hyeung-keun Park ),이혜진 ( Heyjean Lee ),조은정 ( Eun-jung Cho ),채성철 ( Shung C 한국보건정보통계학회(구 한국보건통계학회) 2017 보건정보통계학회지 Vol.42 No.1

Objectives: This study was conducted to investigate economic feasibility of operating Regional Cardiocerebrovascular Centers using cost-benefit analysis. Methods: The current study assessed cost and benefit data collected from nine hospitals which were designated as Regional Cardiocerebrovascular Centers between 2008 and 2010. Results: The operation of Regional Cardiocerebrovascular Centers reduced mortality rate and length of stay due to acute myocardial infarction and stroke and also decreased disability occurred after stroke. The total calculated cost of operating nine Regional Cardiocerebrovascular Centers was 19.85-22.30 billion won and the minimum benefit calculated was 23.98 billion won. Applying the maximum cost and the minimum benefit, the net benefit was 1.68 billion won, which is estimated to 190 million won per Regional Cardiocerebrovascular Center. Conclusions: The present study revealed that the operation of Regional Cardiocerebrovascular Centers was economically beneficial. The benefit would be larger if intangible benefit such as the long-term effects to community hospitals and enhancement of quality of life of patients and their families are considered.

곰팡이에서 분리한 Helicobacter pylori 항균물질의 분리 및 구조규명

남궁준,연승우,백남수,김태한,김영호,김창진,김기원 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-

Helicobacter pylori is a Gram-negative bacterium which causes chronic gastritis and is associated with gastric ulcer, duodenal ulcer and gastric carcinoma. In the process of screening of antibacterial activities against H. pylori from soil microorgani는, fungus No. 60686 was isolated. After fementaion of No. 60686, the antibacterial compound was isolated, purified and followed by extraction of mycelium with orgainic solvents, acetone and ethyl acetate, through silica gel chromatography, LH-20 gel chromatography and HPLC. As a result of the structural analyses of the compound by IR, ^1H- and ^13C-NMR, FAB/Mass spectrophotomer, the compound having the antimicrobial activity was idenfitied as chaetoglobosin A (C_32H_36N_2O_5), a cytochalasan derivative. The antimicrobial activity of chaetoglobosin A was tested against Gram-positive and negative bacteria by paper disk method. Among the test strains of 9 Gram-positive bacteria and 18 Gram-negative bacteria containing 4 H. pylori strains, the growth of 4 H. pylori strains and 3 S. aureus strains (SG 511, 285 and 503) was only inhibited by chaetoglobosin A. Also it was shown that its growth inhibition against H. pylori strains was stronger than that against S. aureus strains at the treatment of the same concentration. Therefore it was concluded that chaetoglobosin A has a specific growth inhibition against H. pylori of the tested bacteria.

차량 간 무선 통신을 위한 거리기반 브로드캐스팅 프로토콜

김태환,김희철,홍원기 대구대학교 정보통신연구소 2006 情報通信硏究 Vol.4 No.1

株式에 대한 投資成果와 인플레이션에 관한 實證的 硏究

金元基 啓明大學校 産業經營硏究所 1980 經營經濟 Vol.12 No.1

The main purpose of this papose is to find out the relationship between stock returns and inflation. That is (1) to find out if stock returns hedges inflation in Korean Stock Market, (2) to see if stock returns are affected by time lead and time lag, (3) to seek how inflation hedges is affected by industry in Korean Stock Market, (4) to compare the U.S. Stock Market with Korean Stock Market in respect in respect of stock returns and inflation. This study concludes as follows: 1. A conclusion about inflation hedges in KSM can not be confirmed due to the low R²(0.0088) found in relationship between stock returns and inflation. Many other factors are involved besides the inflation factor. 2. In relationship between stock price and inflation, time lead allows better prediction than time lag. Especially R²was 14% with three or four month's time lead. 3. Industry surveys are as follows: R??=a+bI?+e? Industries a b t R² D.W Food 1.6515 0.3106 1.7930 0.445 1.6687x paper 0.9809 -0.4127 -1.1064 0.1064 0.0174 Pramarcy 1.3836 -0.4630 -0.7763 0.0087 1.9671x Cement 0.3480 0.9250 1.2934 0.0237 1.2109 Steel 0.6617 0.6135 1.25042 0.0222 1.7679x Eletronics 3.6184 0.3308 0.3577 0.0028 1.7156x Construction -0.5360 2.5299 2.0734* 0.072 1.2897 Trade -1.8712 2.3261 2.7925** 0.1477 1.7904x R??; Rate of return on Industry Market portfolio in month t I ; Rate of inflation in month t ** ; Significant at 1% level *,x ; Significant at 5% level

Mineral trioxide aggregate가 인간치수세포에서 유전자 발현에 미치는 영향

김용범,손원준,이우철,금기연,백승호,배광식 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.3

이 연구에서는 mineral trioxide aggregate (MTA)를 in vitro로 인간치수세포에 적용하였을 때 유전자들의 변화를 조사하였다. 실험군은 MTA를 teflon tube (직경 10 mm 길이 2 mm)에 담아 4시간 경화시킨 후HDPCs에 적용하였고, 대조군은 빈 tub만을 적용하였다. 6, 24, 72시간 후 total RNA를 추출하여 microarray를 이용하여 분석하여, 2배 이상 또는 절반 이하의 변화를 보이는 유전자 중 선택적으로 역전사 중합효소 연쇄반응(reverse transcriptase polymerase chain reaction)을 사용하여 발현을 확인하였다. 24,546개의 유전자 중에서 109개의 유전자가 2배 이상 up-regulation되었으며(예. FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, TUFT1) 69개의 유전자가 50%이하로 down-regulation되었다(예. SMAD6, DCN). MTA는 bio-inert한 재료라기 보다는 치수세포에 다양한 경로로 영향을 주는 재료로 사료된다. 특히 치수세포의 분화와 증식에 관여하는 유전자의 변화에 영향을 주며 석회화 과정에 관여하는 유전자의 변화에 직접적인 영향을 주리라 사료된다. This study investigated the changes in gene expression when mineral trioxide aggregate (MTA) was applied in vitro to human dental pulp cells (HDPCs). MTA in a teflon tube (diameter 10 mm, height 2mm) was applied to HDPCs. Empty tube-applied HDPCs were used as negative control. For microarray analysis, total RNA was extracted at 6, 24, and 72 hrs after MTA application. The results were confirmed selectively by performing reverse transcriptase polymerase chain reaction for genes that showed changes of more than two-fold or less than half. Of the 24,546 genes, 109 genes were up-regulated greater than twofold (e.g., FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, and TUFT1) and 69 genes were downregulated below 50% (e.g., SMAD6 and DCN). These results suggest that MTA, rather than being a bioinert material, may have potential to affect the proliferation and differentiation of pulp cells in various ways.

유한요소법을 이용한 판 구조물의 동적거동 해석 연구

김영권,이기형,박태원,정인성 全北大學校 1995 論文集 Vol.39 No.-

This study discusses the finite element approximation and the choice of elements is dictated by the need to perform accuate for both thin and thick shells and by the complexity of the sensitivity calculations. A number of very accurate plate and shell elements exist for which the sensitivity computation become hopelessly complex. On the other hand, most very simple elements, which would lend themselves to compact and efficient sensitivity computation, do not posses sufficient accuracy for routine use.

이동환경에서 RFID 단말 시스템의 설계 및 구현

김태환,홍원기,이용두 대구대학교 정보통신연구소 2004 情報通信硏究 Vol.3 No.2

RFID는 태그와 리더간 전자기 유도를 통해 객체를 식별하는 무선 통신 기술이다. 이는 객체를 식별하기 위한 인식 거리나 위치 등에 있어서 바코드에 비해 매우 자유로우며 동시에 여러 객체를 인식 할 수 있기 때문에 향후 물류, 유통 산업에서 높은 기술적 가치를 갖는다. 일반적으로, 리더기를 통해 식별된 객체에 대한 상세한 정보를 얻어내기 위해 PML 서버로부터 해당하는 정보를 검색, 추출하기 위한 과정이 요구된다. 그러나 이것은 많은 연산과 서버와의 통신을 요구하기 때문에 자원 제한적인 리더기에는 상당한 부담이 될 수 있다. 본 논문에서는 제한적인 하드웨어 자원을 가진 리더기에서 PML 문서 검색, 추출을 위한 부하를 줄이기 위한 시스템을 제안한다. RFID (Radio Frequency IDdentification) is a wireless communication technology that identifies an object containing a tag through electromagnetic induction. Because it is not restricted in its position or the read range as much as the bar code technology which requires a line-of-sight to identify an object but also able to read several tags at the same time, it is considered to be one of the promising technologies used in the distribution industry in the future. Generally, a process of retrieving and acquiring a corresponding data should be needed to get a detail information about an object identified by a RFID reader. However, it gives a heavy burden on the resource-restricted reader because it requires significant computation and communication tasks. This paper proposes a 글로벌 RFID system that efficiently decreases the computation load to retrieve an acquire a PML document for the resource-restricted reader.

2段曝氣方式에 依한 製紙廢水處理 效果分析

金秀生,池基遠,朴出在 동아대학교 환경문제연구소 1990 硏究報告 Vol.13 No.1

Adapting two step aeration system to a waste water treatment of W-paper manufactory as Full-Scale Plants, we drew a following conclusion from its practical working. 1. Because BOD removal efficiency was 20% in A-Stage, 90% in B-Stage and total removal efficiency was 97%, it worked treatment plant well and was suitable for discharged water standard as well. Because COD removal efficiency was 42% in A-Stage, 71% in B-Stage and the total removal efficiency was 94%, COD control was possible in discharged water quality. 2. Treatment efficiency according to a load capacity was average 20% in 1.401 BOD㎏/㎥·d load of A-Stage and average 90% in 0.273BOD㎏/㎥·d load of B-Stage. 3. Treatment efficiency according to a ration of F/M was 2.657∼5.024㎏ BOD/㎏ MLSS·d in A-Stage and BOD removal efficiency was 16∼26% in the same stage. The ratio of F/M was 0.068∼0.094㎏BOD-㎏MLSS·d and BOD removal efficiency ratio was 85∼94%. Therefore treatment efficiency could be kept stably and volume of aeration tank could be reduced wholly. 4. Treatment efficiency according to MLSS appeared BOD 20%, COD 42%, in A-Stage and removal efficiency appeared BOD 90%, COD 71% in B-Stage. They were suitable for plan condition. 5. Because of working of complemented treatment plant by AB-Process. 20,000,000won a month was saved than the ordinary working cost. So, it was assumed that invested cost could be recollected in 19 months or so consequently.