1-Deoxynojirimycin의 급성독성 및 항균효과

백남수,김영만 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.6

Streptomyces exofoliatus SID9135 균주의 발효배양 여액으로부터 anion(Dowex 1X2-100 Cl^-) 및 cation(Dowex 50X4-100 H^+) 이온교환수지, Sephadex LH20 컬럼, TSK 겔 컬럼크로마토그래피 등으로 저해물질을 정제한 후, 최종적으로 HPLC를 사용하여 α-glucosidase 저해물질인 1-deoxynojirimycin을 순수 분리정제 하였다. IRC 마우스에서의 1-deoxynojirimycin의 급성독성 조사에서 200㎎/㎏의 dose로 경구 투여하여 10일간 관찰하였을 때 치사동물이 전혀 없었으며 체중증가는 대조군과 동일한 양상을 보였다. Agar 희석법으로 20종의 병원균에 대한 1-deoxynojirimycin과 AO-128 및 acarbose의 MIC한 결과 1-deoxynojirimycin과 대조약물인 AO-128 및 acarbose의 농도가 100㎍/ml 이상에서도 20종의 시험균주 모두에 대해 항균효과를 나타내지 않았다. 1-Deoxynojirimycin which is a potent intestinal α-glucosidase inhibitor was purified from the culture broth by ion exchange chromatography, Sephadex LH20 column chromatography, TSK gel chromatography and HPLC respectively. Acute toxcity of 1-deoxynojirimycin, which was loaded through the oral as dose of 200㎎/㎏, was investigated in IRC mouse. None of the tested IRC mice were not dead and increase of body weight showed also the same results in comparison with control mice. The antimicrobial susceptibility of 20 pathogenic strains against 3 antidiabetic compounds (1-deoxynojirimycin, AO-128, acarbose) were obtained by agar dilution method. All of the three antidiabetic compounds had very weak antimicrobial activity (MIC>100 ㎍/ml).

세선화 알고리즘에 기반한 숫자 특징 추출 및 숫자열 인식에 관한 연구

백남수,이은주 한국정보기술응용학회 2001 한국정보기술응용학회학술대회 Vol.2001 No.1

수도 계량기 검침 자동화 시스템 구현

백남수,이은주 大田産業大學校 2002 한밭대학교 논문집 Vol.19 No.-

In this paper, we proposed a new method for automatic inspection of water gauge, and implemented it. We decided meter area for inspection using intensity of input image, and noise removed by labeling and size of label. The numbers were overlapped or not, it was decided by characteristics of numbers, and recognition area was decided by size of area. Finally, we computed the similarity between area of numbers and reference pattern by double template matching, and the number that similarity is the highest with area of numbers was result of recognition.

Streptomyces sp. NS15 배양액에 의한 α-Glucosidase 저해

백남수,김영만 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.6

Streptomyces sp. NS15 균주로부터 비단백질성 α-glucosidase 저해제의 생산을 위해 초기 배지의 최적 pH와 질소원, 탄소원, 금속 이온, 교반속도 및 통기량 등의 효과에 대하여 조사하였다. 배지의 초기 최적 pH는 7.0이었으며 가장 효과적인 질소원과 탄소원은 각각 soybean meal 2.0%(w/v)와 glucose 1.6%(w/v)였으며 금속이온에서는 Fe^+2에서 저해활성에 있어서 약간의 상승효과를 나타낸 반면 Cu^+2은 오히려 활성저하를 보였다. Jar fermentor 배양에서의 교반속도와 통기량은 각각 400rpm과 1vvm에서 효과적이었으며 최적 배지와 최적조건 하에서 4일간 배양한 배양여액의 저해활성이 돼지 소장의 α-glucosidase에 대해 3,200units/ml로 기본배지(CYM)에서 보다 25배 높은 저해활동을 보였고 5일째부터는 저해활성이 점차 감소하는 양상을 보였다. For the production of nonprotein α-glucosidase inhibitor from the Streptomyces sp. NS15 strain, effects of initial optimum pH, nitrogen sources, carbon sources, cationic metal ions, agitation speed and aeration rate were investigated. Initial optimum pH of medium was 7.0. The most effective nitrogen and carbon source were soybean meal 2.90%(w/v) and glucose 1.6%(w/v), respectively. The cationic metal ions had no stimulating effect on inhibitory activity of α-glucosidase except Fe^2+. Agitation speed and aeration rate were effective at 400rpm and 1vvm, respectively. In the jar-fermenter cultivation for 4 days under optimal culture conditions, the culture broth showed the inhibitory activity of 3,200units/ml, which is 25 times higher than that of basic medium(CYM) for porcine intestinal α-glucosidase. The inhibitory activity of α-glucosidase reached about 3,200units/ml after 4 days of cultivation and decreased gradually for a further two days.

Probiotics에 의한 해수양식어의 성장촉진 및 항균효과

백남수,임유범,김영만 한국산업미생물학회 2001 한국미생물·생명공학회지 Vol.29 No.1

해수양식어 전용의 probiotics를 개발하기 위하여 저온성 및 내염성의 특성을 갖는 균주를 김치로부터 분리하였다. 분리한 3종의 균주를 동정한 결과 MG19는 Lactobacillus brevis, MG89는 Enterococcus faecium, MG208는 Lactobacillus plantarum 으로 확인되었다. 분리된 3종 균주의 중화된 농축 배양여액을 사용하여 해수어 병원성세균인 Edwardsiella tarda, Vibrio cholerae, Pseudomonas fluorescens에 대한 항균력을 시험한 결과 대조약물과 비슷한 효과를 나타냈으며 각 분리균주와 해수어 병원성세균과의 혼합배양시 48시간 이후 병원균의 증식이 상당히 저해되었다. 해수어 병원균중 가장 피해를 많이 주는 Edwardsiella tarda와 3종의 분리균주를 동시에 혼합배양 하였을 경우 24시간 이후 Edwardsiella tarda의 증식을 완전히 저해하였다. 또한 3종의 분리균주를 동결건조한 균원료를 사료에 0.1% 첨가하여 넙치에 매일 투여한 후 50일이 경과한 다음 체중 증가를 조사한 결과 대조군에 비해 50%의 증체율을 보였다. 이러한 결과로부터 3종의 분리균주는 해수어 전용 probiotics로서 중요한 역할을 할 것으로 예상된다. For the development of probiotics in aquaculture of marine organisms, three strains having psychrophilic and salt tolerant characteristics were isolated from Kimchi. Among the isolated strains, MG19, MG89 and MG208 were identified as Lactobacillus brevis, Enterococcus faecium and Lactobacillus plantarum, respectively. The neutralized culture broth of isolated strains were tested in order to evaluate the antibacterial activity, which showed high antibacterial activity against Edwardsiella tarda, Vibrio cholerae and Pseudomonas fluorescens. In mixed culture of pathogens and isolated strain, pathogens were significantly inhibited after 2 days of cultivation but the isolated strains showed normal growth. When the Dewardsiellatarda was cultured with three isolated strains, its growth was completely inhibited after 24 hours of cultivation. The effect of isolated three strains as probiotics was investigated based on the changes in body weight of aquacultured flounder. After 50 days feeding trial, it was found that the mean body weight gain of the tested group fed freezedried isolated cells was significantly greater than that of the control group. These results suggest that these isolated strains can play an important role as probiotics in aquaculture.

수도 계량기 검침 자동화 시스템 구현

백남수,이은주 한국정보기술응용학회 2002 한국정보기술응용학회학술대회 Vol.2002 No.1

한국여성의 질에서 분리한 Lactobacillus plantarum MG989와 Lactobacillus fermentum MG901의 Gardnerella vaginalis와 Candida albicans 에 대한 억제효과 및 특성 규명

백남수(Nam-Soo Paek),이윤엽(Youn Yeop Lee),한설화(Seul Haw Han),강창호(Chang-Ho Kang),소재성(Jae-Seong So) 한국생물공학회 2016 KSBB Journal Vol.31 No.1

Vaginitis, also known as vaginal infection and vulvovaginitis, is an inflammation of the vagina and possibly vulva. The three main kinds of vaginitis are bacterial vaginosis, vaginal candidiasis, and trichomoniasis. The purpose of this study was to characterize Lactobacillus plantarum MG989 and L. fermentum MG901 isolated from the vaginas of healthy Korean women in terms of their inhibitory activity against the vaginitis associated pathogens such as Gardnerella vaginalis and Candida albicans. Co-culture experiments showed that the two Lactobacillus strains MG989 and MG901 significantly reduced the viability of G. vaginalis and C. albicans. Also, the two strains were resistant to bile acid up to 1% and their autoaggregation rates were as high as 83.33%. Further studies are underway to demonstrate that the two strains can be applied as pharmaceutical agents for recovering healthy vaginal ecosystem.

식품 및 환경 , 기타 Probiotics 에 의한 해수양식어의 성장촉진 및 항균효과

백남수(Nam Soo Paek),임유범(Yoo Beum Lim),김영만(Young Man Kim) 한국미생물생명공학회 2001 Korean Journal of Applied Microbiology and Biotech Vol.29 No.1

N/A N/A

유포자성 유산균을 이용한 홍삼농축액 개발에 관한 연구

이병혁,백남수,김영만 한경대학교 2005 論文集 Vol.37 No.-

In order to develop the Concentrated Liquid of Red Ginseng with spore forming lactic acid bacteria powder as functional food, Lactobacillus sporogenes which has resistant spore against high temperature, low pH, and artificial bile acid was studied. Jar fermentor culture of Lactobacillus sporogenes under optimal condition showed that the number of spore reached about 20×10^(8)CFU/㎖ and sporulation rate was 97%. Lactobacillus sporogenes powder (3500×10^(8)CFU/㎖) was made from the process such as centrifuge, wash of distilled water and acetone treatment, drying of 2 hours at 55 ℃, pulverizing. The spore of Lactobacillus sporogenes within the Concentrated Liquid of Red Ginseng survived 65% after treatment for 20minute in boiling water. In the treatment of artificial gastric juice(pH 3.0) for 4 hours and artificial bile acid for 8 hours, the survival rate was 50% and 85% respectively. Its storage stability was 70% during the 12 months at room temperature.

생균제 개발을 위한 유산균의 선별 및 동결건조 보호제의 효과

임유범,백남수,김영만 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.5

Probiotics를 개발하기 위하여 저온성 및 내염성의 특성을 갖는 균주를 김치로부터 분리하였다. 분리한 3종의 균주를 동정한 결과 MGl9는 Lactobacillus brevis, MG89는 Enterococcus faecium, MG208은 Lactobacillus plantarum으로 확인되었다. 분리된 3종의 균주에 대한 동결건조 보호제 시험을 한 결과 MGl9는 10% skim milk + l% soluble starch에서 71.4%로 생존율이 가장 높았고 lactose와 mellezitose 에서도 60% 이상의 생존율을 나타냈다. MG89는 solbitol에서 76.2%로 생존율이 가장 높았고 sucrose, xylitol, mannitol 등에서도 66% 이상의 생존율을 나타내었다. MG208은 fructose에서 64.7%의 생존율을 보였다 인공위액에서의 균 생존율을 시험한 결과 lactose, mellezitose, hlycogen, mannose, xylose 등에서70% 정도의 우수한 생존율을 보였다. 이러한 결과로부터 본시험에 사용된 동결건조 보호제 가운데 몇몇은 유산균의 종류에 따라 다르지만 동결건조제품의 제조나 사료첨가제로 제조시 산업적인 이용이 가능할 것으로 사료된다. For the developement of probiotics, three strains haying psychorophilic and salt tolerant characteristics were isolated from Kimchi. Among the isolated strains, MGl9, MG89 and MG208 were identified as Lactobacillus brevis, Enterococcus faecium and Lactobacillus plantarum, respectively. The relationship between cryoprotectant and the viability of freeze dried strains has been studied. The most effective cryoprotectant for MGl9 was found to be 10% skim milk contained 1% soluble starch haying the survival rate of 71.4%. In MG89, 10% skim milk contained 1% sucrose and 10% skim milk contained l% fructose in MG208 were showed effective cryoprotectant having the survival rate 68.9% and 64.7%. respectively These results suggest that these isolated strains can play an important role as probiotics in aquaculture.