Efficacy and Safety of Pueraria lobata Extract in Gray Hair Prevention; A Randomized, Double-Blind, Placebo-Controlled Study

( Seong Jin Jo ),( Hyoseung Shin ),( Seung Hwan Paik ),( Sun Jae Na ),( Ying Ji Jin ),( Won Seok Park ),( Su Na Kim ),( Oh Sang Kwon ) 대한피부과학회 2013 Annals of Dermatology Vol.25 No.2

Graying of hair-a sign of aging-raises cosmetic concerns. Individuals with gray hair often look older than others their age; therefore, some dye their hair for aesthetic purposes. However, hair colorants can induce many problems including skin irritation, allergic reaction and hair-breakage. Objective: This randomized, double- blind clinical trial was performed in order to examine the effects of APHG-1001, a compound including an extract from Pueraria lobata, on graying hair. Methods: A total of 44 female subjects were randomly treated with either APHG-1001 or placebo twice daily for 24 weeks. Using the phototrichogram analysis, a count of newly developed gray hair was estimated. Investigator assessment and subject self-assessment were also performed in order to evaluate the efficacy of the compound. Results: The mean number of newly developed gray hair at 24 weeks was 6.3/cm2 in the APHG-1001 group and 11.4/cm2 in the placebo group; the difference was statistically significant (p<0.05). However, the investigator assessment and subject self-assessment did not show any significant change in the gross appearance of hair grayness by the end of the study. No severe adverse events in either group were observed. Moreover, the incidence of adverse events did not differ between the groups. Conclusion: This clinical trial revealed that APHG-1001, which contains an extract of P. lobata, could prevent the development of new gray hair without any remarkable adverse effects. Thus, it can be considered as a viable treatment option for the prevention of gray hair.

A Survey of the Awareness, Knowledge and Behavior of Hair Dye Use in a Korean Population with Gray Hair

( Jung Eun Kim ),( Hee Dam Jung ),( Hoon Kang ) 대한피부과학회 2012 Annals of Dermatology Vol.24 No.3

Background: Gray hair naturally develops in the process of human aging. Many people with gray hair periodically dye their hair. Hair dyeing products are widely used and they can cause adverse effects. Therefore, the user`s knowledge and recognition about hair dyeing and related side effects are important. Objective: The goal of this study was to lay the foundation for understanding, preventing and treating side effects caused by hair coloring products. Methods: We conducted a questionnaire survey for adult males and females aged over 20 who had gray hair. A total of 500 subjects were included in this study and statistical analysis was performed. Results: Large numbers of the people who had experience with hair dye (233 out of 319 people, 73.0%) did not know about the exact brand name of the hair dye product that they were using. Of 319 hair dye users, 23.8% (76 out of 319) people stated that they experienced side effects. Despite the occurrence of side effects from hair dyeing products, it seems they did not realize the seriousness of the side effects or the need for treatment. Conclusion: It is advisable to introduce a system that enables users to become aware of the ingredients and side effects of hair coloring products and give opportunities for users to become aware of the side effects of hair coloring through education, publicity and publication of an informational booklet.

흰 모발과 흑갈색 모발의 영구염모제 염색 전·후 투과전자현미경 관찰

이수비 ( Su Bee Lee ),장병수 ( Byungsoo Chang ),김영철 ( Young Chul Kim ) 대한미용학회 2021 대한미용학회지 Vol.17 No.4

The current study examined microstructural changes in gray and black-brown hairs before and after permanent hair dyeing using transmission electron microscopy (TEM). A close connection of cuticle cells to the cell membrane complex (CMC) was observed on TEM images of black-brown and gray hairs. A cuticle cell layer comprising the exocuticle and endocuticle was easily distinguished due to the difference in electron density. The cuticle CMC was also composed of two inner and outer β layers with low electron density and one δ layer with high electron density. The cortex was distinguished from the cuticle layer by cuticle CMC and the medulla was distinguished from the cortex. Holes varying in size were observed in the endocuticle of black-brown hair, however their presence in the gray hair was unlikely. The cortical cells of black-brown hair were filled with macrofibrils, melanin granules, and cell debris, with the presence of melanin granules in the intermacrofibrilar matrix and cell debris. The cortex of the gray hair was filled with macrofibrils, and no melanin granules were observed. Dyeing reaction materials were observed as particles with high electron density in the endocuticles, cuticle and cortex CMCs, intermacrofibrilar matrix, and cellular debris in dyed black-brown hair and dyed gray hair. Characteristically, an increase in the thickness of both cuticle and cortex CMCs was observed after dyeing (0.04 µm) compared with that before dyeing (0.02 µm). In addition, in cuticle CMC, the outer β layer was strikingly thickened compared with the inner β layer.

지렁이 자가분해물이 한국인 탈색모발의 회색계열 염색에 미치는 영향

박정아,조영재,강상모 한국미용학회 2018 한국미용학회지 Vol.24 No.3

In coloring gray hair in bleached hair for Korean people, it is hard to color and maintain hair due to hair damage. To solve this problem, the following samples were prepared: a control group with gray hair coloring, a pre-treated group in which hair was colored after earthworms autolysate treatment on bleached hair, mixed group in which hair was dyed with the mixture of earthworms autolysate and hair color . To examine color durability by shampoo frequency (once, 10 times, 20 times) after coloring the bleached hair once and 3 times, L*, a* and b* values were measured. L* and b* values were the highest in the pre-treated group until 20th shampooing after coloring the hair in dark gray (8.11) and bright gray (10.11) once and 3 times, followed by the mixed group and control group. Hair was visually inspected by experts after shampooing the colored hair up to 20 times. In terms of ‘coloring uniformity’, ‘brightness’, ‘glossiness’ and ‘smoothness’, the pre-treated group was the best in both one-time and 3-times bleached hair. In terms of ‘texture roughness’, the control group was the highest. Regarding ‘hair dye deodorization effects’, the mixed group was the greatest. Just like the mechanical measurement, the pre-treated group was the best at an expert group questionnaire. Since the earthworms autolysate treatment enhances hair coloration and color durability during gray hair dyeing, it is reasonable to say that it is commercially valuable.

영구염모제로 처리한 흰 모발과 흑갈색 모발의 구성 원소성분 변화

이수비,장병수,김영철 대한미용학회 2020 대한미용학회지 Vol.16 No.2

In the present study, morphological characteristics and elemental composition of a permanent hair dye mixture were examined using a scanning electron microscope and energy dispersive X-ray spectrometer, respectively. In addition, compositional changes in gray hair before and after permanent hair dyeing were analyzed and compared with those in black-brown hair. SEM micrographs showed the formation of spongy-type holes of various sizes on the surface of the permanent hair dye mixture. EDX results revealed that the permanent hair dye mixture consisted of C, O, N and S elements. Before hair dyeing, the sulfur content of gray hair (3.32%) was found to be lesser than that of the blackbrown hair (4.40%). After hair dyeing, the sulfur content decreased both in the gray and black-brown hairs.

현진건 장편번역소설 「백발」 연구

황정현(Hwang Jung-hyun) 고려대학교 한국학연구소 2012 한국학연구 Vol.42 No.-

본 논문은 현진건의 장편번역소설 백발(1921) 의 원작과 저본을 밝히고, 이를 통해 현진건의 작가론 및 한국 근대번역번안문학 연구의 폭을 넓히고자 했다. 이 소설은 내용으로 미루어 보아 알렉상드르 뒤마의 소설 몽테 크리스토 백작 의 번안이라고 막연히 추정되어 왔으나, 이는 오류이다. 백발 의 원작은 영국 소설가 마리 코렐리의 복수(1886) 이고, 저본은 일본 작가 구로이와 루이코의 번안 백발귀(1893) 이다. 영국 빅토리아 시대 인기 작가였던 마리 코렐리의 작품을 일본의 구로이와 루이코가 번안했고, 당시 ≪조선일보≫의 기자였던 현진건이 구로이와 루이코의 일역본을 다시 우리말로 옮긴 것이다. 백발 의 원작과 저본의 확인은 현진건의 작품 연보를 수정 보완하고 문학 활동을 재조명함으로써, 현진건의 문학 세계를 보다 다각도에서 조망할 가능성을 제시한다.<br/> 또한 본 논문은 이 소설의 연재 및 출간을 둘러 싼 자료를 분석함으로써, 신문기자 현진건의 번역 활동이 소설가 현진건의 문학관과는 별개로 이루어졌음을 밝히는 한편, 이 소설이 지닌 독자 호응 요소에 대해서도 고찰했다. 현진건은 독자층의 범위와 작품의 성격에 따라 원작자명 및 역자명 표기, 작중인물명 설정을 달리하였다. 이는 번역가로서 현진건이 소설가와 신문기자라는 이중의 정체성을 지니고 있었음을 말해 준다. 신문기자의 역할을 극대화하여 번역한 백발 은 당대의 시대적 상황 및 독자들의 요구와 부합하여 인기 있는 대중소설로 자리매김할 수 있었다. This paper proposes to clarify the two sources of Hyun Jin-geon’s full-length translation novel Gray Hair, which are the original text and the adapted version from which Hyun’s novel was translated again, respectively. Accordingly this paper is expected to widen the breadth of research on Hyun’s literature and Korea’s translation/adaptation literature in the modern era. Gray Hair, judging from the content, has been vaguely estimated as adapted from the French novel Le Comte de Monte-Cristo. I argue, however, that the original text of Gray Hair is the British novelist Marie Corelli’s Vendetta (1886) and that Gray Hair was not the direct adaptation from the original but translated from another text that is the Japanese writer Ruiko Kuroiwa’s Gray Haired Ghost (1893). Ruiko Kuroiwa adapted the work of Marie Corelli the popular British writer in<br/> the Victorian era, and Hyun Jin-geon, a newspaper reporter for Chosun Ilbo at that time, translated the Ruiko Kuroiwa’s Japanese adaptation into Korean again.<br/> The clarification of the two sources of Hyun Jin-geon’s Gray Hair―the British original text and the Japanese adaptation as the second-handed source for Korean translation, respectively―suggests the possibility to correct or supplement the chronological listings of Hyun’s literary works and, therefore, overlook his literary world more fully and from different angles. In addition, by analyzing the records and documents surrounding the serial installments in newspaper and publication of Gray Hair, this paper, on one hand, examines that translation work of Hyun as a journalist was made independently of his literary career as a novelist. Concerning the translated text, we can understand that Hyun had different attitude to the target, which he translated, based on certain standards. He arranged different settings for the notation of author's name, translator's<br/> name, and character's names in the translated texts in accordance with the range readership and the artistry of the work. This tells us Hyun has dual identity as a novelist and a newspaper reporter. <br/> On the other hand, the contemporary reader’s response to serial translation novels in newspaper is also investigated in this paper. Such textural and contextual study on Gray Hair is expected to contribute to further efforts to investigate the nature of Korea’s translation and adaptation literature in the modern era.

백모 모구의 유전자 발현 프로파일링을 통한 백모화 기전 분석

김민정 ( Min Jung Kim ),김종란 ( Jong Ran Kim ),윤영민 ( Yeongmin Yoon ),안성관 ( Sung Kwan An ) 한국미용학회 2012 한국미용학회지 Vol.18 No.1

Hair bulb is composed of follicular melanocytes, keratinocytes, and dermal papilla fibroblasts and their relationship play an important role in the morphogenesis, growth and graying of the hair. To investigate the cause of graying hair in molecular levels, in this study, we analyzed changes of gene expression in hair bulb obtained from Korean male aged 20`s-30s`. We identified mRNA expression profiles in between graying and black hair bulb using DNA microarray analysis. We found 67 genes with more than 2-fold changes in their expression levels in graying hair bulb as compared with black hair bulb. Among them, 30 genes were up-regulated and 37 genes were down-regulated. The function of 67 genes turned out to be associated with melanogenesis, cell-cell interaction, regulation of calcium concentration, cytokine, keratin and serine type protease inhibition. These results indicate that graying is modulated by changes of the gene expression pattern in hair bulb, regulating various cellular functions.

Anti-Graying Effect of Pueraria Lobata Root Extract on Stress-Induced Hair Graying

홍민정,박병철,홍용덕,김수나 사단법인 대한화장품학회 2022 대한화장품학회지 Vol.48 No.3

Gray hair is a representative sign of aging. Intrinsic aging, stress, and the external environment cause hair graying. Stress is known to be a major factor in the early onset of hair graying. We previously found that Pueraia lobata root extract (PLRE) can prevent hair graying by promoting melanin formation. However, it remains unknown whether PLRE can prevent hair graying induced by conditions of stress. In this study, we confirmed the effect of PLRE on stress-induced hair graying. A reporter cell line was newly constructed to confirm the expression of microphthalamia-associated transcription factor (MITF), the main transcription factor for melanin production. MITF expression and melanin pigmentation were reduced in human hair follicle tissue treated with the stress hormone cortisol or H2O2 to induce oxidative stress. PLRE treatment restored MITF expression and increased the amount of melanin pigment in the hair follicle. The expression of Tyrosinase related proteins-2 (TRP-2), a melanin synthesis enzyme in the hair follicle, also increased. In conclusion, PLRE can effectively prevent the inhibition of melanin synthesis by stress hormones and oxidative stress.

Management of Greying of Hairs (Sheeb) and Use of Hair Dyes (Khizaab) in Unani Medicine

Rani, Seema Cellmed Orthocellular Medicine and Pharmaceutical 2018 셀메드 (CellMed) Vol.8 No.2

Greying of hairs or Canities (Sheeb) is a hair disorder characterized by premature graying of scalp hair, beard, moustache etc. In Unani system of medicine (USM), the causes of premature greying of hairs, concept behind graying of hairs, principles of treatment, preparations that prevent premature greying of hairs, regimen, systemic and local treatment to stimulate pigmentation and the most interesting point is the use of different type of hair dyes (khizabat) is all illustrated. Classical texts described black, red, blond and white hair dye preparations with formulae and method of use. Initially for the hair graying management, utilizing simple and complex substances from plants, minerals, metals and mixture of these was the main method, which satisfied the desire to change the colour of the hair. With the advancement in chemical science, dyes formulaes, method of formation and application are changed. But due to the awareness about demerits of chemical dyes, people are looking back towards the natural ways to combat hair greying and herbal hair dye is an alternative. This paper is an overview of Unani drugs of local and internal use for hair greying with special attention towards herbal dyes. Most commonly used herbs in khizaab with their actions and constituents has been summarized. This is an effort to globalize the benefits of Unani herbs in hair greying problem. In short, International demand for hair dyes has been steadily growing and there is a wide scope for exploring different aspects of hair greying treatment and dyes in USM.

A case of premature hair graying treated with oral zinc oxide

( Jun Suk Hong ),( Dong Won Lee ),( Moo Kyu Suh ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Premature graying of hair, known as premature canities, is defined as the occurrence of the hair graying before the 25 in Asians. Many pathologic conditions have been discussed about the association with hypothyroidism, vitamin B12 deficiency, vitiligo, progeroid syndromes and medications. We report a case of premature graying hair in a 7-year-old girl. She presented with slowly graying of hair on the vertex of her scalp with a 1-year history. The physical examination revealed a lot of gray colored hair mixed with normal colored hair on the scalp. She didn’t have any family history of premature hair graying and autoimmune diseases including alopecia areata. vitiligo, autoimmune thyroid disease, pernicious anemia and some related rare premature syndrome. The initial laboratory findings were within normal range on serum vitamin B12(763.4pg/ml; normal, 197-771), ferritin(98.32ng/ml; normal, 13-150), iron(67㎍/dl; normal, 33-193) and cooper(129.33㎍/dl; normal, 64-134). Although serum zinc level was lower limit of normal(71.27㎍/dl; normal, 70-120), hair mineral test was showed slight deficiency of zinc level. So zinc supplements(oral zinc oxide 8.5mg/day) applied for six months. After 6 months of treatment with only oral zinc oxide medication, her hair have recovered normal black colored hair.