염산/에탄올로 유도된 급성위염 동물모델에서 치자 에탄올 추출물의 보호 효과

김수현,이진아,이아름,신유옥,박해진,노성수 한국식품영양과학회 2019 한국식품영양과학회지 Vol.48 No.2

This study examined the protective effects of gardenia fruit 50% ethanol extract (GFE) on a 150 mM HCl/60% ethanol-induced acute gastric injury model in mice. The antioxidant activities were evaluated through radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). To evaluate the anti-gastritis effect of GFE, ICR mice were divided into 5 groups: Nor (normal group), Con (gastritis induced by HCl/ethanol with distilled water), SC treatment group (gastritis induced by HCl/ethanol treated with 10 mg/kg sucralfate), GL treatment group (gastritis induced by HCl/ethanol treated with 50 mg/kg GFE), and GH treatment group (gastritis induced by HCl/ethanol treated with 100 mg/kg GFE). The mice were pretreated with the extract (GL, GH) or drug (SC), and after 1 hour, 0.55 mL of 150 mM HCl/60% ethanol (v/v) mixture was administered orally. GFE improved the gastric lesions in a concentration-dependent manner. In addition, GFE inhibited the increased reactive oxygen species and ONOO- through the antioxidant pathway. The antioxidative biomarkers, including nuclear factor-erythroid 2-related factor 2, heme oxygenase-1, superoxide dismutase, catalase, and glutathione peroxidase-1/2, were increased significantly. In addition, the inflammatory related makers, nuclear factor-kappa B p65, inhibitor of nuclear factor kappa B alpha, cyclooxygenase-2, tumor necrosis factor-alpha, and interleukin-1 beta, were increased. GFE significantly inhibited inflammation through the antioxidant pathway. Therefore, GFE has potential for treating acute gastritis. 본 연구는 150 mM HCl/60% 에탄올로 유도된 급성위염 동물 모델에서 치자 50% 에탄올 추출물(GFE)의 보호 효과를 평가하기 위해 수행되었다. 치자 추출물은 DPPH, ABTS 자유라디칼 소거능 실험을 통해 평가한 결과, 높은 항산화 활성을 나타냈다. GFE의 항위염 효과를 평가하기 위해 ICR 마우스를 이용하였고, Nor(정상군), Con(대조군), SC투여군(sucralfate 10 mg/kg 농도), GL투여군(치자 추출물 50 mg/kg 농도), GH투여군(치자 추출물 100 mg/kg 농도) 총 5군으로 나누어 실험을 진행하였다. 치자 추출물 투여 후 위점막을 육안으로 관찰한 결과, 점막 손상을 현저히 감소시켰다. 위 조직에서 산화적 스트레스 바이오마커인 ROS와 ONOO-를 감소시켰다. 또한 위 조직을 western blot을 통해 분석한 결과, Nrf-2, HO-1, SOD, catalase 및 GPx-1/2 항산화 바이오마커들의 발현을 증가시켰으며, NF-κBp65, IκBα, COX-2, TNF-α 및 IL-1β 염증성 인자의 발현을 유의하게 억제하였다. 따라서 치자 50% 에탄올 추출물의 투여가 급성위염 동물실험 모델에서 위염 개선 효과가 있다고 사료된다.

염산/에탄올로 유도된 급성 위염 동물모델에서 십자화과 생즙 발효물의 위점막 보호 효과

박양규,조정휘,최진영,김영필,이상엽,박주헌,오홍근,Park, Yang-Gyu,Cho, Jeong-Hwi,Choi, Jinyoung,Kim, Youngpil,Lee, Sang-yeob,Park, Ju-Hun,Oh, Hong-Geun 한국식품영양학회 2021 韓國食品營養學會誌 Vol.34 No.5

In recent years, there has been an increase in the morbidity of gastritis in Korea due to lifestyle factors mostly changes in eating habits and stress. Gastritis is more likely to progress to gastric cancer, and therefore it is important to prevent and manage gastritis through lifestyle adjustment and treatment at an early stage. In this study, cabbage, which was found to be effective in gastritis, was mixed and fermented with other crucifer plants such as kale and broccoli to evaluate the overall efficacy of fermented brassica puree on alcoholic acute gastritis. Based on our results, fermented brassica puree alleviated gastric injury induced by 150 mM HCl/60% ethanol. In addition, it was confirmed that PGE₂, a gastric mucosal protective factor, was increased, and other positive effects such as an increase of MUC1 and regulation of PKC were observed. The results of this study suggest that fermented brassica puree can relieve acute alcoholic gastritis by regulating PGE and the expression of MUC1, a gene related to mucus secretion, and activating PKC, which is related to mucosal cell activity.

A case of cytomegalovirus-negative M$\acute{e}$n$\acute{e}$trier's disease with eosinophilia in a child

Son, Keun-Hyung,Kwak, Jeong-Ja,Park, Jae-Ock The Korean Pediatric Society 2012 Clinical and Experimental Pediatrics (CEP) Vol.55 No.8

M$\acute{e}$n$\acute{e}$trier's disease is a rare form of acquired gastropathy characterized by giant rugal folds in the stomach and protein-losing gastropathy. Children with M$\acute{e}$n$\acute{e}$trier's disease tend to follow a benign self-limited course with symptoms typically completely resolving within 2 to 10 weeks in contrast to the chronic course in adults. A 9-year-old girl presented with a history of gradually worsening abdominal distension, increasing body weight, and abdominal pain for 2 weeks. Physical examination on admission indicated periorbital swelling, pitting edema in both the legs, and abdominal distension with mild diffuse tenderness and shifting dullness. Laboratory tests on admission showed hypoalbuminemia, hypoproteinemia, and peripheral eosinophilia. The test result for anti-cytomegalovirus immunoglobulin M was negative. Increased fecal alpha 1 anti-trypsin excretion was observed. Radiological findings showed massive ascites and pleural effusion in both the lungs. On gastroscopy, large gastric folds, erythema, erosion, and exudation were noted in the body and fundus of the stomach. Microscopic findings showed infiltration of eosinophils and neutrophils in the gastric mucosa. Her symptoms improved with conservative treatment from day 7 of hospitalization and resolved completely.

Cotoneaster mongolicus Pojark. 추출물의 HCl/ethanol로 유발된 위염 mice에 대한 보호효과

최정원,이진아,신미래,박해진,노성수,Choi, Jeong Won,Lee, Jin A,Shin, Mi-Rae,Park, Hae-jin,Roh, Seong-Soo 한국생약학회 2022 생약학회지 Vol.53 No.3

Cotoneaster mongolicus Pojark. (CM), in the family Rosaceae is an endemic plant to the Mongolian region (its name: Moнroл чapraй). In Mongolia, Cotoneaster species as a crude drug is mainly used for inflammatory diseases, diarrhea, and stomach indigestion. In this study, we evaluated the gastro-protective activity underlying mechanism of CM. For in vivo experiments, mice were divided into 5 groups; normal mice (Normal), gastritis mice (Control), gastritis mice treated with sucralfate 10 mg/kg (SC), gastritis mice treated with CM 100 mg/kg (CML), gastritis mice treated with CM 200 mg/kg (CMH). Gastritis was provoked by HCl/ethanol (60% ethanol in 150 mM HCl). After oral administration of each drug, HCl/ethanol was orally administered 90 mins later to induce gastritis. CM alleviated the damage to the gastric mucosa caused. As a result of confirming the expression of protein in gastric tissue through western blot, CM significantly reduced the expression of NF-κB activated due to gastritis. Also, it significantly modulated the Nrf2-Keap1 pathway. These results indicate that CM not only inhibits the nuclear metastasis of NF-𝛋B but also modulates the Nrf2-Keap1 pathway to relieve inflammation of the gastric mucosa.

소화기 질환 동물모델에서 평위산(平胃散)의 염증 완화 효과

정세영 ( Seyoung Jung ),정성은 ( Sung Eun Jung ),김지혜 ( Ji Hye Kim ),함성호 ( Seong Ho Ham ),양웅모 ( Woong Mo Yang ),권보인 ( Bo-in Kwon ) 대한본초학회 2020 大韓本草學會誌 Vol.35 No.2

Objectives : Pyeongwi-san is widely used in Korean medicine for acute indigestion or gastrodynia. As a therapeutic agent for digestive diseases of modern people, in order to confirm the mechanism of Pyeongwi-san on digestive tract disease and the difference of therapeutic efficacy between its formulation, a comparative efficacy test was conducted on digestive tract disease animal model. Methods : For LPS enteritis animal model, male SD rats were intraorally treated with different formulation types of Pyeongwi-san, and then intraperitoneally administered LPS one hour later to induce enteritis. After 5 hours, blood was collected and TNFα, IL-1β, IL-6 and PGE2 were confirmed by ELISA. For acute gastritis animal model, male SD rats were intraorally treated with different formulation types of Pyeongwi-san according to the prescribed concentration, and then intraorally administered 60% ethanol and 150 mM HCl one hour later to induce acute gastritis. After 5 hours, blood was collected and TNFα ,IL-6 were confirmed by ELISA. Results : In the LPS-administered enteritis animal model, Pyeongwi-san decreased TNFα, IL-1β, PGE2 and especially IL-6. Pyeongwi-san also decreased IL-6 in acute gastritis animal model. In addition, there was no significant difference in efficacy between the two formulations when compared with inflammatory markers. Conclusions : The efficacy of Pyeongwi-san was confirmed in the inflammatory markers related to digestive inflammatory diseases, and the efficay between two formulations of Pyeongwi-san was relatively similar. Further studies are needed to investigate the new applicability of Pyeongwi-san on different inflammatory diseases that have similar inflammation markers identified in this experiment.

육군자탕의 정제 개발과 성분함량 및 약리효과 평가

김명진 ( Myoung-jin Kim ),최혜민 ( Hye-min Choi ),유병우 ( Byung-woo Yu ),홍영주 ( Young-ju Hong ),라채숙 ( Chae-suk Ra ),김민주 ( Min-ju Kim ),김정옥 ( Jung-ok Kim ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.1

Objective : Yukgunja-tang is one of the herbal prescriptions widely used for functional indigestion. In this study, we evaluated the pharmacological effect through the Yukgunja-tang formulation development. Methods : The RAW 264.7 cells were pretreated with Yukgunja-tang tablet (YGJT-T : 50, 100 and 200 ㎍/㎖) and then stimulated with lipopolysaccharide (LPS : 500 ng/㎖). Cell viability, inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) and inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured. Also, ICR mice induced acute gastritis by oral administration of 150 mM HCl in 60% ethanol. The YGJT-T (30 ㎎/㎏) was pretreated for 3 days, and 150 mM HCl in 60% ethanol was orally administered 1 hour after the last drug treatment. Mice were sacrificed 1 hour after oral administration of 150 mM HCl in 60% ethanol. The gastric mucosa was observed, and inflammatory cytokines in the gastric tissue were measured. Results : The marker components of YGJT-T were determined by simultaneous analysis using HPLC. In RAW 264.7 cells, pretreatment of YGJT-T was non-toxic and inhibited the production of inflammatory mediators such as NO and PGE2 and suppressed inflammatory cytokines. In addition, pretreatment of YGJT-T improved bleeding and edema due to gastric lesions caused by acute gastritis and suppressed inflammatory cytokines. Conclusion : In summary, our results confirmed that treatment with YGJT-T has anti-inflammatory and anti-gastritis effects in vitro and in vivo. Therefore, in this study, YGJT-T could support a potential strategy for the prevention and treatment of gastritis.

증숙 횟수에 따른 천마 추출물의 급성 위염 개선효과

이아름(Ah Reum Lee),권오준(O Jun Kwon),노정숙(Jeong Sook Noh),노성수(Seong-Soo Roh) 한국식품과학회 2016 한국식품과학회지 Vol.48 No.6

본 연구는 증숙 횟수에 따른 천마 추출물의 경구 투여가 산화방지 효과를 통한 위 점막 손상 억제에 미치는 효과를 평가하는 실험이다. 증숙 횟수를 달리한 천마 추출물을 준비하여 DPPH, ABTS 라디칼 소거능, total phenol, flavonoid, gastrodin, gastrodigenin 성분 분석 결과, 1회 증숙 천마와 9회 증숙 천마 추출물간의 급성 위염 개선 효능 평가가 가능할 것으로 판단되었다. HCl/ethanol로 유발된 급성 위염 동물 모델에 GE1과 GE9 (100 mg/kg body weight)과 sucralfate (10 mg/kg body weight)를 HCl/ethanol 처리 전 경구 투여하였다. 그리고 이를 정상군과 대조군과 비교분석하였다. 급성 위염 개선 효능 실험에서 증숙 천마 추출물의 섭취는 HCl/ethanol로 유발된 위 점막 손상 마우스에서 위 조직의 육안적 손상을 감소시켰다. 9회 증숙 천마 추출물의 섭취는 위 점막 손상마우스에서 혈청의 ROS와 조직의 ONOO−를 감소시켰고, 위 조직에서 염증성 매개인자인 TNF-α 또한 감소시켰다. 결론적으로 9회 증숙한 천마의 경구투여는 1회 증숙한 천마에 비하여 효과적으로 위 점막 손상을 억제하였다. 따라서 9회 증숙 천마 추출물의 투여가 급성 위염 유발 마우스 모델에서 위 점막 손상 억제에 효과가 있다고 사료된다. This study aimed to explore the protective effect of Gastrodia elata (GE) in an HCl/ethanol induced acute gastritis model by differing the steaming time. The samples GE1 (GE by steaming for 1 time) and GE9 (GE by steaming for 9 times), were selected based on the results of HPLC analysis, free radical scavenging activities, and total phenol and flavonoid contents. To evaluate the anti-inflammatory effect of GE, ICR mice were divided into 5 groups; normal mice(Nor), gastritic mice with distilled water (Con), gastritic mice with 100 mg/kg GE1, gastritic mice with 100 mg/kg GE9 and gastritic mice with 10 mg/kg sucralfate (SC). HCl/ethanol-induced gastric mucosal injury was markedly improved by GE9 treatment as observed during histological evaluation. The increased reactive oxygen species levels in the serum were diminished by GE9 treatment. Furthermore, peroxynitrite levels of the stomach tissue were decreased in the GE9-treated group. The analyses of stomach proteins indicated that GE9 treatment effectively reduced inflammatory cytokine levels as compared to that by GE1 treatment. These results suggest that GE9 improves health during acute gastritis.

위령탕 혼합단미연조엑스의 항염증 효과

김명진,김세진,남원희,임현희,전영희,손수미,김정옥 대한한의학방제학회 2020 大韓韓醫學方劑學會誌 Vol.28 No.4

Objectives : Wiryeong-tang (WRT) is a traditional herbal medicine used to treat kidney-related diseases. However, the anti-inflammatory and anti-gastritis effect of Wiryeong-tang was not well known. Therefore, we experimented to confirmed the anti-inflammatory and anti-gastritis effects of Wiryeong-tang. Methods : The RAW 264.7 cells were pre treated with Wiryeong-tang mix soft extract (WRT-mse; 50, 100 and 200 ㎍/mL) for 1 hrs, and then incubated with lipopolysaccharide (LPS; 500 ng/mL). Cell viability was measured by the MTT method, and nitric oxide (NO) was measured with griess reagent. In addition, pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). For anti-gastritis effect in vivo, acute gastritis was induced using 150 mM HCl/60% ethanol used ICR mice. WRT-mse (133 mg/kg) was pre treated for 3 days and then treated with 150 mM HCl/60% ethanol 1 hrs later. Then gastritis was observed and inflammatory cytokines in the gastric tissue was measured. Results : The 8 marker components of the WRT-mse were determined by simultaneous analysis using HPLC. WRT-mse was not toxic and inhibited pro-inflammatory cytokines such as IL-1β, IL-6 and TNF-α at NO production, protein and mRNA levels. Also, it was confirmed that WRT-mse improved bleeding and edema in gastritis, and suppresses inflammatory cytokines. Conclusion : In summary, our results suggest that the treatment of the WRT-mse reduced and improved the 150 mM HCl/60% ethanol induced acute gastritis and the inflammation caused by LPS stimulation in RAW 264.7 cells. Therefore, this study may provide useful drug or clinical evidence for WRT-mse to prevent inflammation.

흑삼 열수 추출물의 항산화 효과를 통한 염산/에탄올로 유발된 위염 억제 작용

김민영(Min Yeong Kim),권오준(O Jun Kwon),노정숙(Jeong Sook Noh),노성수(Seong-Soo Roh) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.9

백삼을 9회 증숙 및 건조 과정 후 제조한 흑삼의 항산화 효과 급성위염 유발 모델에서 점막 보호 효과를 확인하기 위하여 증숙 횟수별 추출한 시료를 통해 실험을 진행하였다. 백삼과 흑삼의 증숙 횟수에 따른 추출물의 성분을 분석한 결과 백삼과 비교하여 흑삼 추출물에서 모두 유효성분의 양이 증가하는 것을 확인하였고, 특히 9회 반복 과정을 거친 BG군에서 가장 높은 성분 함량을 보였다. 또한, 항산화능을 확인하기 위하여 실시된 DPPH, ABTS free radical 소거 활성에서도 BG군의 억제율이 가장 높게 나타났으며, 총페놀과 총플라보노이드의 함량도 가장 높게 나타났다. 이러한 결과를 토대로 백삼과 9번 증숙 과정을 거친 흑삼을 HCl/에탄올로 유도한 급성위염 마우스에 투여한 결과 BG군에서 위 점막의 손상은 대조군과 비교하여 유의한 감소를 보였으며, 또한 백삼과 비교하여 9회 증숙한 흑삼에서 유의한 감소를 보였다. 혈액에서 측정한 활성산소종의 수치에서도 대조군과 비교하여 9회 증숙 흑삼에서 유의한 감소를 보였고, 백삼과 비교하여 뛰어난 항산화 효과를 나타내었다. 산화적 스트레스의 증가로 발생한 사이토카인의 발현을 확인한 결과에서도 COX-2, TNF-α와 IL-6의 경우 대조군보다 유의한 감소를 보였고, 백삼과 비교하여도 유의한 감소를 보였다. 그러므로 9회 증숙한 흑삼에서는 백삼과 비교하여 유효성분 함량의 증가가 발생하여 뛰어난 항산화력을 보이고, 이에 따라 염증성 사이토카인의 발현을 억제시키므로 9회 증숙한 흑삼은 항산화 효과에 따른 급성위염 유발모델에서 점막 보호 효과가 있다고 생각한다. Black ginseng (BG) obtained by a 9-fold steaming process of Panax ginseng has been reported to have anti-oxidative, anti-obesity, and anti-diabetes effects. The current study evaluated the protective effect of BG by steaming time in an HCl/ethanol-induced acute gastritis model. BG was divided into four samples according to steaming-drying processing (Gin1, Gin3, Gin6, and BG). High performance liquid chromatography analysis, free radical scavenging activity, and total phenol and flavonoid contents were examined in ginseng and four BG samples. Compared with ginseng, BG showed a stronger radical scavenging effect and higher contents of total phenol and flavonoids. To evaluate the anti-gastritic effect of BG, mice were distributed into five groups: normal mice (N), acute gastritic mice with distilled water (CON), acute gastritic mice with 100 mg/kg of ginseng (Gin0), acute gastritic mice with 100 mg/kg of BG (BG), and acute gastritic mice with 10 mg/kg of sucralfate (SC). After 1 hour of pre-treatment with water, extracts (Gin0 and BG), or drug (SC), experimental groups except for N were orally administered 0.5 mL of 150 mM HCl/60% ethanol (v/v) mixture. Blood was collected 1 hour later from the heart, and gastric tissue was harvested. Reactive oxygen species (ROS) levels were measured in serum, and related protein expression was examined by Western blot assay. In HCl/ethanol-induced acute gastritic mice, treatment with ginseng or BG improved mucosal damage in the histological evaluation. The serum ROS level significantly decreased in the BG-treated group compared with the CON group. Furthermore, expression of inflammatory cytokines significantly decreased in the BG-treated group compared with the CON group. Based on these results, antioxidant and anti-gastritic activities of ginseng were enhanced by streaming-drying processing, in part due to an increase in biological active compounds.