소화기 질환 동물모델에서 평위산(平胃散)의 염증 완화 효과 = Comparative Efficacy Study of Pyeongwi-san(Pingwei-san) of Digestive Tract Disease Model in Rat

다국어 초록 (Multilingual Abstract)

Objectives : Pyeongwi-san is widely used in Korean medicine for acute indigestion or gastrodynia. As a therapeutic agent for digestive diseases of modern people, in order to confirm the mechanism of Pyeongwi-san on digestive tract disease and the difference of therapeutic efficacy between its formulation, a comparative efficacy test was conducted on digestive tract disease animal model.
Methods : For LPS enteritis animal model, male SD rats were intraorally treated with different formulation types of Pyeongwi-san, and then intraperitoneally administered LPS one hour later to induce enteritis. After 5 hours, blood was collected and TNFα, IL-1β, IL-6 and PGE2 were confirmed by ELISA. For acute gastritis animal model, male SD rats were intraorally treated with different formulation types of Pyeongwi-san according to the prescribed concentration, and then intraorally administered 60% ethanol and 150 mM HCl one hour later to induce acute gastritis. After 5 hours, blood was collected and TNFα ,IL-6 were confirmed by ELISA.
Results : In the LPS-administered enteritis animal model, Pyeongwi-san decreased TNFα, IL-1β, PGE2 and especially IL-6. Pyeongwi-san also decreased IL-6 in acute gastritis animal model. In addition, there was no significant difference in efficacy between the two formulations when compared with inflammatory markers.
Conclusions : The efficacy of Pyeongwi-san was confirmed in the inflammatory markers related to digestive inflammatory diseases, and the efficay between two formulations of Pyeongwi-san was relatively similar. Further studies are needed to investigate the new applicability of Pyeongwi-san on different inflammatory diseases that have similar inflammation markers identified in this experiment.

참고문헌 (Reference)

1 이진아, "한약 처방 25종에 대한 항염증 효능 비교 연구" 대한한방부인과학회 23 (23): 101-111, 2010

2 박상현, "평위산과 그 구성약물 조합이 위장관, 간손상 및 혈압에 미치는 영향" 경희대학교 대학원 2000

3 하성룡, "평위산 변증 설문지와 생체신호의 상관성 연구" 경희대학교 대학원 2009

4 정혜경, "우리나라 소화기질환 환자의 질병비용 추계" 대한소화기학회 58 (58): 323-331, 2011

5 김신정, "염산-에탄올에 의해 유발된 흰쥐 위염에 대한 생강 및 법제생강 추출물의 억제작용" 한국식품영양과학회 41 (41): 1528-1533, 2012

6 이지선, "생강의 약물대사효소 CYP3A4 저해 성분" 대한약학회 48 (48): 2004

7 곽진숙, "생강 건조 분말의 임신 및 멀미에 의한 오심, 구토 개선 기능성에 대한 체계적 고찰" 한국영양학회 47 (47): 45-50, 2014

8 조소연, "감초성분 Carbenoxolone disodium의 항 위염 효과" 한국독성학회 23 (23): 165-172, 2007

9 이경진, "厚朴과 土厚朴의 소장운동에 미치는 영향에 대한 연구" 대한본초학회 26 (26): 75-81, 2011

10 Bujanda L, "The effects of alcohol consumption upon the gastrointestinal tract" 95 (95): 3374-3382, 2000

11 Bonamin F, "The effect of a minor constituent of essential oil from Citrus aurantium : the role of β-myrcene in preventing peptic ulcer disease" 212 : 11-19, 2014

12 National Institute of Food and Drug Safety Evaluation, "The Korean Herbal Pharmacopoeia" 1252-, 2017

13 Cho MR, "Targeting Interleukin-17 and Th17 in Immune Inflammatory Diseases" 33 : 17-26, 2013

14 Lee BJ, "Studies on the Efficacy of Machili Cortex in the Digestive System" 25 (25): 278-292, 1994

15 Hong SA, "Studies on Alkaloid of Glycyrrhiza glabra L. IV. -Anti-ulcer Activity of Licorice Alkaloid Fraction" 5 (5): 149-152, 1969

16 서민준, "Regulatory mechanism of IL-6 production by β-eudesmol, a constituent of Pyungweesan" 원광대학교 대학원 2009

17 Jeong SI, "Regulation of contractile activity by magnolol in the rat isolated gastrointestinal tracts" 59 (59): 183-188, 2009

18 Hwang DH, "Nephropathy bangyak happyeon" Young Rimsa 269-270, 2011

19 Hermann GE, "LPS-induced suppression of gastric motility relieved by TNFR : Fc construct in dorsal vagal complex" 283 (283): G634-G639, 2002

20 Liu Y, "Knockdown of GRP78 promotes apoptosis in pancreatic acinar cells and attenuates the severity of cerulein and LPS induced pancreatic inflammation" 9 (9): e92389-, 2014

21 Chen G, "Isoliquiritigenin, a flavonoid from licorice, plays a dual role in regulating gastrointestinal motility in vitro and in vivo" 23 (23): 498-506, 2009

22 Atreya R, "Involvement of IL-6 in the pathogenesis of inflammatory bowel disease and colon cancer" 28 (28): 187-196, 2005

23 Satoh K, "Interaction of betaeudesmol with Na+, K(+)-ATPase : inhibition of K(+)-pNPPase activity" 44 (44): 993-995, 1992

24 Satoh K, "Inhibition of H+, K+-ATPase by hinesol, a major component of so-jutsu, by interaction with enzyme in the E1 state" 59 (59): 881-886, 2000

25 Stallmach A, "Induction and modulation of gastrointestinal inflammation" 19 (19): 438-441, 1998

26 Tsutsui H, "Importance of Kupffer cells in the development of acute liver injuries in mice" 15 (15): 7711-7730, 2014

27 Kidd M, "IL1beta-and LPS-induced serotonin secretion is increased in EC cells derived from Crohn's disease" 21 (21): 439-450, 2009

28 Tanaka T, "IL-6 in Inflammation, Immunity, and Disease" 6 (6): 3-, 2014

29 Thiago Mello Moraes, "Healing Actions of Essential Oils from Citrus aurantium and d-Limonene in the Gastric Mucosa: The Roles of VEGF, PCNA, and COX-2 in Cell Proliferation" 한국식품영양과학회 16 (16): 1162-1167, 2013

30 Marx W, "Ginger-Mechanism of Action in Chemotherapy-induced Nausea and Vomiting : A Review" 57 (57): 141-146, 2017

31 Beynon AL, "Ghrelin inhibits LPSinduced release of IL-6 from mouse dopaminergic neurones" 10 : 40-, 2013

32 Rozza AL, "Gastroprotective mechanisms of Citrus lemon (Rutaceae) essential oil and its majority compounds limonene and β-pinene: involvement of heat-shock protein-70, vasoactive intestinal peptide, glutathione, sulfhydryl compounds, nitric oxide and prostaglandin E" 189 (189): 82-89, 2011

33 Ernst E, "Efficacy of ginger for nausea and vomiting : a systematic review of randomized clinical trials" 84 (84): 367-371, 2000

34 Lee HI, "Effetcs of administration pyung yoe san to the secreting cell in the gastric mucosa of the rat" 1 (1): 20-24, 1976

35 Moraes TM, "Effects of limonene and essential oil from Citrus aurantium on gastric mucosa : role of prostaglandins and gastric mucus secretion" 180 (180): 499-505, 2009

36 Saberi F, "Effect of ginger on relieving nausea and vomiting in pregnancy : a randomized, placebocontrolled trial" 3 (3): e11841-, 2014

37 Huh J, "Donguibogam. 1: Internal External side" Yeo Gangchulpansa 336-337, 2005

38 Sun J, "D-Limonene : safety and clinical applications" 12 (12): 259-264, 2007

39 Laiakis EC, "Comparison of mouse urinary metabolic profiles after exposure to the inflammatory stressors γ radiation and lipopolysaccharide" 177 (177): 187-199, 2012

40 Lee SR, "Comparison of Pharmacological Effects of Magnoliae officinalis , Magnolia, and Machilus Barks" 17 (17): 199-205, 1986

41 Ashizawa T, "Clinical significance of interleukin-6 (IL-6) in the spread of gastric cancer: role of IL-6 as a prognostic factor" 8 (8): 124-131, 2005

42 Giacosa A, "Can nausea and vomiting be treated with ginger extract?" 19 (19): 1291-1296, 2015

43 Marx W, "Can ginger ameliorate chemotherapy-induced nausea? Protocol of a randomized double blind, placebocontrolled trial" 14 : 134-, 2014

44 Bribi N, "Antinociceptive and Anti-Inflammatory Effects of Total Alkaloid Extract from Fumaria capreolata" 2015 : 736895-, 2015

45 Ramakrishna YG, "Aegle marmelos fruit extract attenuates Helicobacter pylori Lipopolysaccharide induced oxidative stress in Sprague Dawley rats" 15 : 375-, 2015

46 National Health Insurance Service Health Insurance Policy Institute, "2013 Health Insurance Statistical Yearbook" 2014