Supercapacitors based on a nitrogen doped hierarchical porous carbon fabricated by self‑activation of biomass: excellent rate capability and cycle stability

Zhijian Zhang,Jingjing He,Xingchang Tang,Yuling Wang,Binbin Yang,Kunjie Wang,Deyi Zhang 한국탄소학회 2019 Carbon Letters Vol.29 No.6

Energy and environmental are always two major challenges for the sustainable development of the modern human being. For avoiding the serious environmental pollution caused in the fabrication process of porous carbon, a popular energy storage material, we reported a facile, green and activating agent free route hereby directly carbonizing a special biomass, Glebionis coronaria. A nitrogen doped hierarchical porous carbon with a specific surface area of up to 1007 m2 g−1 and a N doping content of up to 2.65 at.% was facilely fabricated by employing the above route. Benefiting from the peculiarly hierarchical porous morphology, enhanced wettability and improved conductivity, the obtained material exhibits superior capacitance performance, which capacitance reaches up to 205 F g−1 under two-electrode configuration, and no capacitance loss is observed after 5000 cycles. Meanwhile, the capacitance retention of the obtained material arrives up to 95.0% even under a high current density of 20 A g−1, illuminating its excellent rate capability. The fabricated nitrogen-doped hierarchical porous carbon with larger capacitance than commercial activated carbon, excellent rate capability and cycle stability is an ideal cost-efficient substitution of commercial activated carbon for supercapacitor application.

Supercapacitors based on a nitrogen doped hierarchical porous carbon fabricated by self-activation of biomass: excellent rate capability and cycle stability

Zhang Zhijian,He Jingjing,Tang Xingchang,Wang Yuling,Yang Binbin,Wang Kunjie,Zhang Deyi 한국탄소학회 2019 Carbon Letters Vol.29 No.6

Energy and environmental are always two major challenges for the sustainable development of the modern human being. For avoiding the serious environmental pollution caused in the fabrication process of porous carbon, a popular energy storage material, we reported a facile, green and activating agent free route hereby directly carbonizing a special biomass, Glebionis coronaria. A nitrogen doped hierarchical porous carbon with a specific surface area of up to 1007 m2 g−1 and a N doping content of up to 2.65 at.% was facilely fabricated by employing the above route. Benefiting from the peculiarly hierarchical porous morphology, enhanced wettability and improved conductivity, the obtained material exhibits superior capacitance performance, which capacitance reaches up to 205 F g−1 under two-electrode configuration, and no capacitance loss is observed after 5000 cycles. Meanwhile, the capacitance retention of the obtained material arrives up to 95.0% even under a high current density of 20 A g−1, illuminating its excellent rate capability. The fabricated nitrogen-doped hierarchical porous carbon with larger capacitance than commercial activated carbon, excellent rate capability and cycle stability is an ideal cost-efficient substitution of commercial activated carbon for supercapacitor application.

A Fibrin Matrix Promotes the Differentiation of EMSCs Isolated from Nasal Respiratory Mucosa to Myelinating Phenotypical Schwann-Like Cells

Zhijian Zhang,Qian Chen,Jinbo Liu,Qinghua He,Yuepeng Zhou,Genbao Shao,Xianglan Sun,Xudong Cao,Aihua Gong,Ping Jiang 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.3

Because Schwann cells perform the triple tasks of myelination, axon guidance and neurotrophin synthesis, they are candidates for cell transplantation that might cure some types of nervous-system degenerative diseases or injuries. However, Schwann cells are difficult to obtain. As another option, ectomesenchymal stem cells (EMSCs) can be easily harvested from the nasal respiratory mucosa. Whether fibrin, an important transplantation vehicle, can improve the differentiation of EMSCs into Schwann-like cells (SLCs) deserves further research. EMSCs were isolated from rat nasal respiratory mucosa and were purified using anti-CD133 magnetic cell sorting. The purified cells strongly expressed HNK-1, nestin, p75NTR, S-100, and vimentin. Using nuclear staining, the MTT assay and Western blotting analysis of the expression of cell-cycle markers, the proliferation rate of EMSCs on a fibrin matrix was found to be significantly higher than that of cells grown on a plastic surface but insignificantly lower than that of cells grown on fibronectin. Additionally, the EMSCs grown on the fibrin matrix expressed myelination-related molecules, including myelin basic protein (MBP), 2’,3’-cyclic nucleotide 3’-phosphodiesterase (CNPase) and galactocerebrosides (GalCer), more strongly than did those grown on fibronectin or a plastic surface. Furthermore, the EMSCs grown on the fibrin matrix synthesized more neurotrophins compared with those grown on fibronectin or a plastic surface. The expression level of integrin in EMSCs grown on fibrin was similar to that of cells grown on fibronectin but was higher than that of cells grown on a plastic surface. These results demonstrated that fibrin notonly promoted EMSC proliferation but also the differentiation of EMSCs into the SLCs. Our findings suggested that fibrin has great promise as a cell transplantation vehicle for the treatment of some types of nervous system diseases or injuries.

Effects of Chicory Inulin on Serum Metabolites of Uric Acid, Lipids, Glucose, and Abdominal Fat Deposition in Quails Induced by Purine-Rich Diets

Zhijian Lin,Bing Zhang,Xiaoqing Liu,Rui Jin,Wenjing Zhu 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.11

Inulin, a group of dietary fibers, is reported to improve the metabolic disorders. In the present study, we investigated the effects of chicory inulin on serum metabolites of uric acid (UA), lipids, glucose, and abdominal fat deposition in quail model induced by a purine-rich diet. In this study, 60 male French quails were randomly allocated to five groups: CON (control group), MOD (model group), BEN (benzbromarone-treated group), CHI-H (high-dosage chicory inulin-treated group), and CHI-L (low-dosage chicory inulin-treated group). The serum UA level was significantly increased in the model group from days 7 to 28, as well as triglyceride (TG) and free fatty acid (FFA) increased later in the experimental period. The abdominal fat ratio was increased on day 28. Benzbromarone can decrease UA levels on days 14 and 28. The high and low dosage of chicory inulin also decreased serum UA levels on days 7, 14, and 28. The abdominal fat ratio, activity, and protein of acetyl-CoA carboxylase (ACC) were decreased in chicory inulin-treated groups. The activities of xanthine oxidase (XOD) and fatty acid synthase (FAS) were increased in the model group and decreased in the benzbromarone and chicory inulin groups. This study evaluated a quail model of induced hyperuricemia with other metabolic disorders caused by a high-purine diet. The results indicated that a purine-rich diet might contribute to the development of hyperuricemia, hypertriglyceridemia, and abdominal obesity. Chicory inulin decreased serum UA, TG, and abdominal fat deposition in a quail model of hyperuricemia by altering the ACC protein expression and FAS and XOD activities.

Circ_0074027 regulates the progression of non-small cell lung cancer via miR-1304-5p/GJB2 axis

Zhang Jie,Zhu Yangli,Lin Ya,Hu Yuli,Kuang Zhijian 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.1

Background Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors; circular RNA (circRNA) has been shown to be involved in the advance of NSCLC. However, the function of circ_0074027 in NSCLC has not been fully studied. Objective Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of circ_0074027, microRNA-1304-5p (miR-1304-5p) and gap junction protein beta 2 (GJB2) in NSCLC. Cell counting kit-8 assay, thymidine analog 5-ethynyl-2′-deoxyuridine (EdU) assay, transwell assay, and flow cytometry were designed to explore cell function. Western blot examined the protein levels of Bax, Bcl-2 and GJB2. The relationship between miR-1304-5p and circ_0074027 or GJB2 was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. The xenotransplantation model was established to study the role of circ_0074027 in vivo. Results circ_0074027 and GJB2 are significantly high-expressed in NSCLC tissues and cells. Silencing circ_0074027 reduced the proliferation, migration and invasion of NSCLC cells, and promoted cell apoptosis. MiR-1304-5p is a target of circ_0074027. Silencing circ_0074027 inhibited the malignant behavior of NSCLC cells by releasing miR-1304-5p. In addition, GJB2 is a target of miR-1304-5p. Overexpression of GJB2 partially restored the inhibitory effect of miR-1304-5p on cell malignant behavior. Animal experiments confirmed the anti-tumor effect of silence circ_0074027 in vivo. Conclusion Circ_0074027 regulates the expression of GJB2 by regulating miR-1304-5p, thereby promoting the development of NSCLC. Our study provides a novel targeted therapy for NSCLC.

An Improved MITM Attack Against NTRU

Zhijian Xiong,Jinshuang Wang,Yanbo Wang,Tao Zhang,Liang Chen 보안공학연구지원센터 2012 International Journal of Security and Its Applicat Vol.6 No.2

Establishment and evaluation of a murine anb3-integrinexpressing cell line with increased susceptibility to Foot-and-mouth disease virus

Wei Zhang,Kaiqi Lian,Fan Yang,Yang Yang,Zhijian Zhu,Zixiang Zhu,Weijun Cao,Ruoqing Mao,Ye Jin,Jijun He,Jianhong Guo,Xiangtao Liu,Haixue Zheng 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

Integrin anb3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and rolesof anb3 integrin, a stable CHO-677 cell line expressing the murine anb3 heterodimer (designated as “CHO-677-manb3” cells) wasestablished using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits an and b3 were detected at the gene andprotein levels by polymerase chain reaction (PCR) and indirect immunofluorescent assay (IFA), respectively, in the CHO-677-manb3 cellline at the 20th passage, implying that these genes were successfully introduced into the CHO-677 cells and expressed stably. A plaque-formingassay, 50% tissue culture infective dose (TCID50), real-time quantitative reverse transcription-PCR, and IFA were used to detect the replicationlevels of Foot-and-mouth disease virus (FMDV) in the CHO-677-manb3 cell line. After infection with FMDV/O/ZK/93, the cell line showeda significant increase in viral RNA and protein compared with CHO-677 cells. These findings suggest that we successfully established a stableanb3-receptor-expressing cell line with increased susceptibility to FMDV. This cell line will be very useful for further investigation of anb3integrin, and as a cell model for FMDV research.

A Fibrin Matrix Promotes the Differentiation of EMSCs Isolated from Nasal Respiratory Mucosa to Myelinating Phenotypical Schwann-Like Cells

Chen, Qian,Zhang, Zhijian,Liu, Jinbo,He, Qinghua,Zhou, Yuepeng,Shao, Genbao,Sun, Xianglan,Cao, Xudong,Gong, Aihua,Jiang, Ping Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.3

Because Schwann cells perform the triple tasks of myelination, axon guidance and neurotrophin synthesis, they are candidates for cell transplantation that might cure some types of nervous-system degenerative diseases or injuries. However, Schwann cells are difficult to obtain. As another option, ectomesenchymal stem cells (EMSCs) can be easily harvested from the nasal respiratory mucosa. Whether fibrin, an important transplantation vehicle, can improve the differentiation of EMSCs into Schwann-like cells (SLCs) deserves further research. EMSCs were isolated from rat nasal respiratory mucosa and were purified using anti-CD133 magnetic cell sorting. The purified cells strongly expressed HNK-1, nestin, $p75^{NTR}$, S-100, and vimentin. Using nuclear staining, the MTT assay and Western blotting analysis of the expression of cell-cycle markers, the proliferation rate of EMSCs on a fibrin matrix was found to be significantly higher than that of cells grown on a plastic surface but insignificantly lower than that of cells grown on fibronectin. Additionally, the EMSCs grown on the fibrin matrix expressed myelination-related molecules, including myelin basic protein (MBP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and galactocerebrosides (GalCer), more strongly than did those grown on fibronectin or a plastic surface. Furthermore, the EMSCs grown on the fibrin matrix synthesized more neurotrophins compared with those grown on fibronectin or a plastic surface. The expression level of integrin in EMSCs grown on fibrin was similar to that of cells grown on fibronectin but was higher than that of cells grown on a plastic surface. These results demonstrated that fibrin not only promoted EMSC proliferation but also the differentiation of EMSCs into the SLCs. Our findings suggested that fibrin has great promise as a cell transplantation vehicle for the treatment of some types of nervous system diseases or injuries.

In Vitro Activity of the Novel Tetracyclines, Tigecycline, Eravacycline, and Omadacycline, Against Moraxella catarrhalis

Sun Xiang,Zhang Bo,Xu Guangjian,Chen Junwen,Shang Yongpeng,Lin Zhiwei,Yu Zhijian,Zheng Jinxin,Bai Bing 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.3

Background: Tigecycline, eravacycline, and omadacycline are recently developed tetracyclines. Susceptibility of microbes to these tetracyclines and their molecular mechanisms have not been well elucidated. We investigated the susceptibility of Moraxella catarrhalis to tigecycline, eravacycline, and omadacycline and its resistance mechanisms against these tetracyclines. Methods: A total of 207 non-duplicate M. catarrhalis isolates were collected from different inpatients. The minimum inhibitory concentrations (MICs) of the tetracyclines were determined by broth microdilution. Tigecycline-, eravacycline-, or omadacycline-resistant isolates were induced under in vitro pressure. The tet genes and mutations in the 16S rRNA was detected by PCR and sequencing. Results: Eravacycline had a lower MIC50 (0.06 mg/L) than tigecycline (0.125 mg/L) or omadacycline (0.125 mg/L) against M. catarrhalis isolates. We found that 136 isolates (65.7%) had the tetB gene, and 15 (7.2%) isolates were positive for tetL; however, their presence was not correlated with high tigecycline, eravacycline, or omadacycline (≥1 mg/L) MICs. Compared with the initial MIC after 160 days of induction, the MICs of tigecycline or eravacycline against three M. catarrhalis isolates increased ≥eight-fold, while those of omadacycline against two M. catarrhalis isolates increased 64-fold. Mutations in the 16S rRNA genes (C1036T and/or G460A) were observed in omadacycline-induced resistant isolates, and increased RR (the genes encoding 16SrRNA (four copies, RR1-RR4) copy number of 16S rRNA genes with mutations was associated with increased resistance to omadacycline. Conclusions: Tigecycline, eravacycline, and omadacycline exhibited robust antimicrobial effects against M. catarrhalis. Mutations in the 16S rRNA genes contributed to omadacycline resistance in M. catarrhalis.

Design on a new oil well test shock absorber under impact load

Wang, Yuanxun,Zhang, Peng,Cui, Zhijian,Chen, Chuanyao Techno-Press 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.28 No.3

Continuous operation of test and measurement is a new operating technique in the petroleum exploitation, which combines perforation with test and measurement effectively. In order to measure the original pressure of stratum layer exactly and prevent testing instrument from being impaired or damaged, a suitable shock absorber is urgently necessary to research. Based on the attempt on the FEM analysis and experiment research, a new shock absorber is designed and discussed in this paper. 3D finite element model is established and simulated accurately by LS-DYNA, the effect and the dynamic character of the shock absorber impact by half sinusoidal pulse force under the main lobe frequency are discussed both on theoretics and experiment. It is shown that the new designed shock absorber system has good capability of shock absorption for the impact load.