Molecular Cloning, Tissue Distribution and Expression of Porcine y⁺L Amino Acid Transporter-1

Zhi, Ai-min,Zhou, Xiang-yan,Zuo, Jian-jun,Zou, Shi-geng,Huang, Zhi-yi,Wang, Xiao-lan,Tao, Lin,Feng, Ding-yuan Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human $y^{+}$LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $y^{+}$LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat $y^{+}$LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest $y^{+}$LAT1 mRNA abundance and the lung had the lowest $y^{+}$LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine $y^{+}$LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.

Prediction methods on tunnel-excavation induced surface settlement around adjacent building

Ding, Zhi,Wei, Xin-jiang,Wei, Gang Techno-Press 2017 Geomechanics & engineering Vol.12 No.2

With the rapid development of urban underground traffic, the study of soil deformation induced by subway tunnel construction and its settlement prediction are gradually of general concern in engineering circles. The law of soil displacement caused by shield tunnel construction of adjacent buildings is analyzed in this paper. The author holds that ground surface settlement based on the Gauss curve or Peck formula induced by tunnel excavation of adjacent buildings is not reasonable. Integrating existing research accomplishments, the paper proposed that surface settlement presents cork distribution curve characters, skewed distribution curve characteristics and normal distribution curve characteristics when the tunnel is respectively under buildings, within the scope of the disturbance and outside the scope of the disturbance. Calculation formulas and parameters on cork distribution curve and skewed distribution curve were put forward. The numerical simulation, experimental comparison and model test analysis show that it is reasonable for surface settlement to present cork distribution curve characters, skewed distribution curve characteristics and normal distribution curve characteristics within a certain range. The research findings can be used to make effective prediction of ground surface settlement caused by tunnel construction of adjacent buildings, and to provide theoretical guidance for the design and shield tunnelling.

Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b^0,+ Amino Acid Transporter in Lantang Pigs

Zhi, Ai-Min,Feng, Ding-Yuan,Zhou, Xiang-Yan,Zou, Shi-Geng,Huang, Zhi-Yi,Zuo, Jian-Jun,Ye, Hui,Zhang, Chang-Ming,Dong, Ze-Min,Liu, Zhun Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.8

Cationic amino acid transporter $b^{0,+}AT$ (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine $b^{0,+}AT$. Based on the sequence of porcine $b^{0,+}AT$ deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $b^{0,+}AT$ was isolated. The porcine $b^{0,+}AT$ cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse $b^{0,+}AT$, respectively. Real-time RT-PCR indicated porcine $b^{0,+}AT$ transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest $b^{0,+}AT$ mRNA abundance while the muscle had the lowest (p<0.05). Along the longitudinal axis, the ileum had the highest $b^{0,+}AT$ mRNA abundance while the colon had the lowest (p<0.05). The $b^{0,+}AT$ mRNA level was highest on day 7 and 90 in the duodenum (p<0.05). It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05). There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05). The strongest $b^{0,+}AT$ expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).

Research on Preparation of Sheath-Core Bicomponent Composite Ion Exchange Fibers and Absorption Properties to Metal Ion

Zhi Jia Ding,Lu Qi,Jian Zhong Ye 한국고분자학회 2008 Macromolecular Research Vol.16 No.1

Research on Preparation of Sheath-Core Bicomponent Composite Ion Exchange Fibers and Absorption Properties to Metal Ion

Ding, Zhi-Jia,Qi, Lu,Ye, Jian-Zhong The Polymer Society of Korea 2008 Macromolecular Research Vol.16 No.1

Based on the sheath-core bicomponent composite fibers with modified polystyrene (PS) and the modified polypropylene (PP), composite fibers obtained were further cross-linked and sulphonated with chlorosulphonic acid to produce strong acidic cation ion exchange fibers. The structures of the fibers obtained were characterized using Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC) etc. The optimal technology of the fibers obtained is discussed. The static absorption capacity of the sheath-core bicomponent composite cation exchange fibers for $Zn^{2+}$, $Cu^{2+}$ was determined. The absorption kinetics and major factors affecting the absorption capacities of $Zn^{2+}$, $Cu^{2+}$ were studied, and its chemical stability and regenerating properties were probed. The results suggest that cation exchange fibers with better mechanical properties and higher exchange capability were obtained. Moreover, this type of ion exchange fiber has good absorption properties and working stability to various metal ions. Hence, they have higher practicability.

The Analysis on the present situation and problems of augmented reality books for children

Ding Zhi Bo,Seung-keun Song,Hun Choi,Eunyoung Choi 한국콘텐츠학회 2018 한국콘텐츠학회 ICCC 논문집 Vol.2018 No.12

서비스디자인 개선을 위한 사례분석

딩즈보(Zhi-Bo Ding),송승근(Seung-Keun Song) 한국콘텐츠학회 2018 한국콘텐츠학회논문지 Vol.18 No.11

본 연구는 해외 관광객들의 문화욕구를 분석하고 감천문화마을에서 제공할 수 있는 문화 서비스 요소를 조사하여 문화 서비스를 개선하는 구체적인 방책을 제시하고 궁극적으로 감천문화마을을 우수한 국제 관광 문화 브랜드를 육성하는 것을 목적으로 한다. 외국관광객들의 문화욕구가 무엇인지 알아보기 위해 도시 재생이 진행된 곳 가운데 감천문화마을을 대상으로 사례 연구를 수행하였다. 연구수행 기간은 4개월이었으며 6개국 외국관광객을 대상으로 문헌조사, 인터뷰, 참여관찰을 통해 기초자료를 수집하였다. 자료 분석은 도시재생, 문화욕구, 서비스 디자인 이론을 토대로 내용분석을 실시하였다. 본 연구는 감천문화마을의 5가지 문화 서비스 목표를 제시하였다. 첫째, 현지 특색문화의 전시 및 홍보, 둘째, 문화상품의 개발과 판매, 셋째, 좋은 관광이미지 부각, 넷째, 특이한 먹거리 제공, 다섯째, 문화체험 최적화이다. 본 연구를 통해 감천문화마을에 대한 문화서비스 향상에 도움을 줄 것으로 기대된다. The purpose of this study is to analyze the cultural needs of foreign tourists, to investigate the cultural service elements that can be provided in ‘Gamcheon Culture Village’, to suggest concrete policy to improve cultural services, and ultimately to cultivate excellent international tourism culture brands in ‘Gamcheon Culture Village’. In order to find out the cultural needs of foreign tourists, we conducted a case study on ‘Gamcheon Culture Village’ among the places where the urban regeneration proceeded. The study period was four months, and basic data were collected from foreign tourists from six countries through literature reviews, interviews and participation observation. Analysis of data was based on urban regeneration, cultural desire and service design theory, and we conducted content analysis. This study presented five cultural service goals of ‘Gamcheon Culture Village’. First, it displays and promotes local characteristic culture. Second, it develops and sells cultural products. Third, it brings up a good tourist image. Fourth, it provides an unusual food. Fifth, cultural experience optimization. Through this study, it is expected to help improve the cultural service of ‘Gamcheon Culture Village’.

Deduction and exploration of the evolution and function of vertebrate GFPT family

Wei Si-ang,Xu Ran,Ji Yu-yao,Ding Zhi-wen,Ding Zhi-wen 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Glutamine-fructose-6-phosphate aminotransferase (GFPT) is a key factor in the hexosamine metabolism pathway. It regulates the downstream factor O-GlcNAc to change cell function and plays an important role in the metabolism and immune process of tissues and organs. However, the evolutionary relationship of GFPT family proteins in vertebrates has not been elucidated. Objective: To deduce and explore the evolution and function of vertebrate GFPT family. Methods: 18 GFPT sequences were obtained from Homo sapiens (H. sapiens), Trachypithecus francoisi (T. francoisi), Mus musculus (M. musculus), Rattus norvegicus (R. norvegicus), Gallus gallus (G. gallus), Zootoca vivipara (Z. vivipara), Xenopus tropicalis (X. tropicalis), Danio rerio (D. rerio), Rhincodon typus (R. typus), Plasmodium relictum from National Center for Biotechnology Information (NCBI). The physical and chemical characteristics and molecular evolution of GFPT family proteins and nucleic acid sequences were analyzed by ClustalX2, Gene Doc, MEGA-X, SMART, Datamonkey, R etc. RESULTS: Based on the neighbor-joining (NJ) phylogenetic tree and evolution fingerprints, GFPT family members of vertebrates can be divided into two groups: the GFPT1 group and the GFPT2 group. Seven positive selection sites were identified by IFEL and integrated methods mixed effects model of evolution (MEME) and fixed effects likelihood (REL). Finally, we predicted 28 phosphorylation sites and 18 ubiquitousness sites in the human GFPT1 sequence, 10 phosphorylation sites, and five ubiquitousness sites in GFPT2. Gene ontology (GO) analyzes the protein molecules and KEGG signaling pathways of vertebrates interacting with GFPT family proteins. Conclusions: Our work confirmed that higher animals GFPT family may have differentiated GFPT1 and GFPT2, which meets their own functional needs. This knowledge answers the question what the origin and evolution of GFPT family in vertebrates and provided the basis for disease treatment and function research of GFPT protein.

Fault Classification Method for Inverter Based on Hybrid Support Vector Machines and Wavelet Analysis

Zhi-kun Hu,Wei-hua Gui,Chun-hua Yang,Peng-cheng Deng,Steven X. Ding 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.4

A new classification method for fault waveform is proposed based on discrete orthogonal wavelet transform (DOWT) and hybrid support vector machine (hybrid SVM) for fault type of a three-phase voltage inverter. The waveforms of output voltage obtained from the faulty inverter are decomposed by DOWT into wavelet coefficient matrices, through which we can obtain singular value vectors acted as features of time-series periodic waveforms. And then a multi-classes classification method based on a new Huffman Tree structure is presented to realize 1-v-r SVM strategy. The extracted features are applied to hybrid SVM for determining fault type. Compared to employing the structure based on ordinary binary tree, the superiority of the proposed SVM method is shown in the success of fault diagnosis because the average Loo-correctness of the SVM based on Huffman tree structure exceed the general SVM 3.65%, and the correctness reaches 99.6%.

Full-scale testing and modeling of the mechanical behavior of shield TBM tunnel joints

Ding, Wen-Qi,Peng, Yi-Cheng,Yan, Zhi-Guo,Shen, Bi-Wei,Zhu, He-Hua,Wei, Xin-Xin Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.45 No.3

For shield TBM (Tunnel Boring Machine) tunnel lining, the segment joint is the most critical component for determining the mechanical response of the complete lining ring. To investigate the mechanical behavior of the segment joint in a water conveyance tunnel, which is different from the vehicle tunnel because of the external loads and the high internal water pressure during the tunnel's service life, full-scale joint tests were conducted. The main advantage of the joint tests over previous ones was the definiteness of the loads applied to the joints using a unique testing facility and the acquisition of the mechanical behavior of actual joints. Furthermore, based on the test results and the theoretical analysis, a mechanical model of segment joints has been proposed, which consists of all important influencing factors, including the elastic-plastic behavior of concrete, the pre-tightening force of the bolts and the deformations of all joint components, i.e., concrete blocks, bolts and cast iron panels. Finally, the proposed mechanical model of segment joints has been verified by the aforementioned full-scale joint tests.