Epidemiological Investigation of Asymptomatic Dogs with Leishmania Infection in Southwestern China Where Visceral Leishmaniasis is Intractable

Gui-Hua Zhao,Kun Yin,Wei-Xia Zhong,Ting Xiao,Qing-Kuan Wei,Yong Cui,Gong-Zhen Liu,Chao Xu,Hong-Fa Wang 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.6

Heishui county, located in northwest Sichuan province, southwestern China, is an endemic area of zoonotic visceral leishmaniasis (VL) and is the most intractable area. VL is never destroyed in it. Asymptomatic dogs (Leishmania parasites have been diagnosed but clinically healthy) are considered to be a potential reservoir host in zoonotic VL area, and most can lead to infection of individuals, that is a new challenge for controlling VL in humans. The present study aimed to assess the Leishmania infection rate of asymptomatic dogs in Heishui county. Total 105 asymptomatic domestic dogs were gathered from 4 districts in Heishui county to investigate the infection rate with serological and molecular methods based on ELISA and kinetoplast minicircle DNA(kDNA) PCR, respectively. Out of 105 dogs, 44 (41.9%) were positive by more than 1 method; 21 (20.0%) were positive by ELISA, and 30 (28.6%) were positive by kDNA-PCR. Our study showed that Leishmania infection of domestic dogs which is clinically healthy is prevalent in the studied district, and the asymptomatic dogs infected by Leishmania may be the primary reason for the prevalence of visceral leishmaniasis in the area.

Analysis of Indoleamine 2-3 Dioxygenase (IDO) and EGFR Co-expression in Breast Cancer Tissue by Immunohistochemistry

Bi, Wei-Wei,Zhang, Wei-Hua,Yin, Gui-Hua,Luo, Hong,Wang, Shou-Qin,Wang, Hongran,Li, Chao,Yan, Wei-Qun,Nie, De-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: To determine the amount of co-expression of IDO and EGFR in breast cancer patients. Materials and Methods:In order to obtain the distribution of co-expression of IDO and EGFR in breast cancer, we tested 110 breast cancer paraffin tissue blocks with immunohistochemical methods. Then we investigated the relationship between the diagnostic and pathologic characteristics (tumor size, lymph node status, histologic grade, the gene expression of ER, PR, HER2, p53, Ki67 and PCNA) with the situation of co-expression of IDO and EGFR by reviewing the medical records of 32 breast cancer patients. Results: Among 110 breast cancers, 32 cases demonstrated IDO and EGFR co-expression (29.1%), IDO and EGFR synchronous co-expression being found in 19.1% and asynchronous in 10.0%. Conclusions: IDO and EGFR were co-expressed in breast cancer, including synchronous and asynchronous co-expression. The results suggest that considering IDO and EGFR as two indicators for breast cancer treatment or prognosis analysis provides a potential option of individual treatment for the portion of breast cancer patients with co-expression of IDO and EGFR.

Recent advances in the food application of electrospun nanofibers

Wei-Hua Han,Xiao Li,Gui-Feng Yu,Bin-Chang Wang,Lin-Peng Huang,Jingkai Wang,Yun-Ze Long 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

Electrospinning (e-spinning) has excellent advantages in producing functional and versatile nanofibersand electrospun (e-spun) nanofibers have a variety of potential applications, including in food industry. This paper reviews the recent progress of e-spinning and food-related e-spun nanofibers, covering ediblenanofibers (natural materials and food ingredients), active food packaging (antibacterial packaging,antioxidant packaging, high temperature and humidity resistant packaging), food filtration membranes,and food sensors. Additionally, we discussed the new trend of combining nanomaterials with food andthe challenges of applying e-spinning in food science. The results indicated that e-spinning is a promisingand efficient alternative for the production of daily and functional food.

The In-Cylinder Flow Field Measurement by PIV with Tracing Particle of Diesel Fuel and It`s visualization

( Gui Hua Liang ),( Xi Yan Gao ),( Xu He ),( Wei Guo Liu ),( Yan Pei Sun ) 한국액체미립화학회 2001 한국액체미립화학회 학술강연회 논문집 Vol.2001 No.-

A Piperidine Alkaloid and Limonoids from Arisaema decipiens, a Traditional Antitumor Herb Used by the Dong People

Fu-Wei Zhao,Min Luo,Yue-Hu Wang,Ma-Lin Li,Gui-Hua Tang,Chun-Lin Long 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.11

A new piperidine alkaloid and three known tetranortriterpenoids were isolated from the methanol extracts of the rhizomes of Arisaema decipiens Schott (Araceae) and their chemical structures were identified as (-)-(2R*,3S*,6S*)-N,2-dimethyl-3-hydroxy-6-(9-phenylnonyl) piperidine (1), 6-deacetylnimbin (2), 28-deoxonimbolide (3) and nimbin (4). The N-methylated derivative (1a) of 1 was synthesized. Compound 1 exhibited weak inhibitory activity against the MCF-7 cell line, while compound 1a showed potential inhibitory activity against the MCF-7 cell line with an IC50 value of 4.6 μM and weak inhibitory activity against K562 and SK-OV-3 cells. This plant in genus Arisaema is firstly reported as the source of limonoids that are considered a natural antitumor herbal medicine.

Influence of APP Quality on the Success of Mobile Health App in China : Evaluating Moderation Effects of Familiarity

Jun-Wei Cao,Gui-Hua Zhang,Dae-Wan Kim 한국디지털융합학회 2021 IJICTDC Vol.6 No.2

The service quality of mobile health apps is a crucial factor affecting users’ satisfaction. For analyzing the influence of service quality of mobile health apps on users’ satisfaction in China, a hypothetical model with three dimensions and eight variables was established. Then this hypothetical model was combined with the model of mobile health service quality and hypotheses were proposed based on the actual situations according to the updated D&M information system success (UMDISS) theory. Questionnaires were issued to collect data and the SEM-PLS method was used to valid the hypothetical model and hypotheses. The reliability and security of the system quality of mobile health apps positively impacted the users’ satisfaction. The cooperation and personalization of service quality had a significant impact on the users’ willingness to use mobile health apps. Through multi-group analyses, it was found that the relationship between mobile health app system quality, users’ satisfaction, and the use of mobile health apps differed under different familiarities with mobile health apps. This study is expected to provide theoretical guidance for the marketing of mobile health apps.

Protective Effect of Ultra Low Molecular Weight Heparin on Glutamate-Induced Apoptosis in Cortical Cells

Tian-Gui Yu,Qing-Zhu Zhang,Zhi-Guo Zhang,Wei-Wei Wang,Sheng-Li Ji,Guan-Hua Du 연세대학교의과대학 2008 Yonsei medical journal Vol.49 No.3

Purpose: To investigate the effect of ultra low molecular weight heparin (ULMWH) on glutamate induced apoptosis in rat cortical cells and to explore the possible mechanisms. Materials and Methods: Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis was first analyzed with Hoechst 33258 and then confirmed by DNA fragmentation. The concentration of free intracellular calcium ([Ca²+]i) was determined with fura-2/AM fluorometry. The expression of Bcl-2 family protein and caspase-3 were evaluated with Western blot. Results: Typical apoptotic morphological change in rat cortical cells treated with 100μmol/L glutamate for 24h was detected by Hoechst 33258 staining, which was then confirmed by the DNA ladder of agarose gel electrophoresis. The apoptotic rate of the glutamate treated cells was up to 33.21%, and 24 h of treatment with glutamate increased [Ca²+]i, down-regulated Bcl-2 expression, up-regulated Bax expression, and increased caspase-3 activation in rat cortical cells. Our research demonstrated that ULMWH pretreatment can prevent the glutamate- induced apoptosis, attenuate the increase of [Ca²+]i not only in medium containing Ca²+ but also in Ca²+-free medium, up-regulate the expression of Bcl-2, down-regulate the expression of Bax, and decrease caspase-3 activation. Conclusion: ULMWH has neuroprotective capacity to antagonize glutamate-induced apoptosis in cortical cells, through decrease of Ca²+ release and modulation of apoptotic processes.

Research on a Mobile-aware Service Model in the Internet of Things

( An Jian ),( Gui Xiao-lin ),( Yang Jian-wei ),( Zhang Wen-dong ),( Jiang Jin-hua ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.5

Collaborative awareness between persons with various smart multimedia devices is a new trend in the Internet of Things (IoT). Because of the mobility, randomness, and complexity of persons, it is difficult to achieve complete data awareness and data transmission in IoT. Therefore, research must be conducted on mobile-aware service models. In this work, we first discuss and quantify the social relationships of mobile nodes from multiple perspectives based on a summary of social characteristics. We then define various decision factors (DFs). Next, we construct a directed and weighted community by analyzing the activity patterns of mobile nodes. Finally, a mobile-aware service routing algorithm (MSRA) is proposed to determine appropriate service nodes through a trusted chain and optimal path tree. The simulation results indicate that the model has superior dynamic adaptability and service discovery efficiency compared to the existing models. The mobile-aware service model could be used to improve date acquisition techniques and the quality of mobile-aware service in the IoT.

Clinical Outcomes of Basaloid Squamous Cell Carcinoma of the Esophagus: A Retrospective Analysis of 142 Cases

Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.

A Two-stage State Transition Algorithm for Constrained Engineering Optimization Problems

Jie Han,Chun-hua Yang,Xiaojun Zhou,Wei-Hua Gui 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.2

In this study, a state transition algorithm (STA) is investigated into constrained engineering design optimization problems. After an analysis of the advantages and disadvantages of two well-known constraint-handling techniques, penalty function method and feasibility preference method, a two-stage strategy is incorporated into STA, in which, the feasibility preference method is adopted in the early stage of an iteration process whilst it is changed to the penalty function method in the later stage. Then, the proposed STA is used to solve three benchmark problems in engineering design and an optimization problem in power-dispatching control system for the electrochemical process of zinc. The experimental results have shown that the optimal solutions obtained by the proposed method are all superior to those by typical approaches in the literature in terms of both convergency and precision.